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The map label for this gene is sigD

Identifier: 16078710

GI number: 16078710

Start: 1716493

End: 1717257

Strand: Direct

Name: sigD

Synonym: BSU16470

Alternate gene names: 16078710

Gene position: 1716493-1717257 (Clockwise)

Preceding gene: 16078709

Following gene: 16078711

Centisome position: 40.72

GC content: 42.22

Gene sequence:

>765_bases
ATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGACTT
AATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACG
ATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCTTGAAAAATTTGACCCCAGCCGGGACTTAAAATTTGATACC
TACGCCTCGTTTAGAATTCGCGGCGCAATCATAGACGGGCTTCGTAAAGAAGATTGGCTGCCCAGAACCTCGCGCGAAAA
AACAAAAAAGGTTGAAGCAGCAATTGAAAAGCTTGAACAGCGGTATCTTCGGAATGTATCGCCCGCGGAAATTGCAGAGG
AACTCGGAATGACGGTACAGGATGTCGTGTCAACAATGAATGAAGGTTTTTTTGCAAATCTGCTGTCAATTGATGAAAAG
CTCCATGATCAAGATGACGGGGAAAACATTCAAGTCATGATCAGAGATGACAAAAATGTTCCGCCTGAAGAAAAGATTAT
GAAGGATGAACTGATTGCACAGCTTGCGGAAAAAATTCACGAACTCTCTGAAAAAGAACAGCTGGTTGTCAGTTTGTTCT
ACAAAGAGGAGTTGACACTGACAGAAATCGGACAAGTATTAAATCTTTCTACGTCCCGCATATCTCAGATCCATTCAAAG
GCATTATTTAAATTAAAGAATCTGCTGGAAAAAGTGATACAATAA

Upstream 100 bases:

>100_bases
GGCCGGACGATAGAATTTGAACCGAAGTCCTGCATGCTGCATATTCGAACTGTTAAACAAGGTGAAAAAACGATTTAATT
AAGGTATTAGGGGGATAACA

Downstream 100 bases:

>100_bases
TGAATTTCATGGTTAGCGAGTGAAAAACATGTCAACACTATTATGGCTTTTAAGCTTTATGCTCCACGGCGTTCTTCTGT
ACGCTGTCATTATCCTGTAT

Product: RNA polymerase sigma factor SigD

Products: NA

Alternate protein names: Sigma-28

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MQSLNYEDQVLWTRWKEWKDPKAGDDLMRRYMPLVTYHVGRISVGLPKSVHKDDLMSLGMLGLYDALEKFDPSRDLKFDT
YASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQRYLRNVSPAEIAEELGMTVQDVVSTMNEGFFANLLSIDEK
LHDQDDGENIQVMIRDDKNVPPEEKIMKDELIAQLAEKIHELSEKEQLVVSLFYKEELTLTEIGQVLNLSTSRISQIHSK
ALFKLKNLLEKVIQ

Sequences:

>Translated_254_residues
MQSLNYEDQVLWTRWKEWKDPKAGDDLMRRYMPLVTYHVGRISVGLPKSVHKDDLMSLGMLGLYDALEKFDPSRDLKFDT
YASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQRYLRNVSPAEIAEELGMTVQDVVSTMNEGFFANLLSIDEK
LHDQDDGENIQVMIRDDKNVPPEEKIMKDELIAQLAEKIHELSEKEQLVVSLFYKEELTLTEIGQVLNLSTSRISQIHSK
ALFKLKNLLEKVIQ
>Mature_254_residues
MQSLNYEDQVLWTRWKEWKDPKAGDDLMRRYMPLVTYHVGRISVGLPKSVHKDDLMSLGMLGLYDALEKFDPSRDLKFDT
YASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQRYLRNVSPAEIAEELGMTVQDVVSTMNEGFFANLLSIDEK
LHDQDDGENIQVMIRDDKNVPPEEKIMKDELIAQLAEKIHELSEKEQLVVSLFYKEELTLTEIGQVLNLSTSRISQIHSK
ALFKLKNLLEKVIQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1788231, Length=226, Percent_Identity=39.3805309734513, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1789098, Length=212, Percent_Identity=28.3018867924528, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): RPSD_BACSU (P10726)

Other databases:

- EMBL:   M20144
- EMBL:   AL009126
- PIR:   C55216
- RefSeq:   NP_389529.1
- ProteinModelPortal:   P10726
- SMR:   P10726
- EnsemblBacteria:   EBBACT00000003837
- GeneID:   938482
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16470
- NMPDR:   fig|224308.1.peg.1650
- GenoList:   BSU16470
- GeneTree:   EBGT00070000031774
- HOGENOM:   HBG745096
- OMA:   LYYYEGL
- PhylomeDB:   P10726
- ProtClustDB:   PRK07670
- BioCyc:   BSUB:BSU16470-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02479
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 29468; Mature: 29468

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLNYEDQVLWTRWKEWKDPKAGDDLMRRYMPLVTYHVGRISVGLPKSVHKDDLMSLGM
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHH
LGLYDALEKFDPSRDLKFDTYASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RYLRNVSPAEIAEELGMTVQDVVSTMNEGFFANLLSIDEKLHDQDDGENIQVMIRDDKNV
HHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
PPEEKIMKDELIAQLAEKIHELSEKEQLVVSLFYKEELTLTEIGQVLNLSTSRISQIHSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
ALFKLKNLLEKVIQ
HHHHHHHHHHHHCC
>Mature Secondary Structure
MQSLNYEDQVLWTRWKEWKDPKAGDDLMRRYMPLVTYHVGRISVGLPKSVHKDDLMSLGM
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHH
LGLYDALEKFDPSRDLKFDTYASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RYLRNVSPAEIAEELGMTVQDVVSTMNEGFFANLLSIDEKLHDQDDGENIQVMIRDDKNV
HHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
PPEEKIMKDELIAQLAEKIHELSEKEQLVVSLFYKEELTLTEIGQVLNLSTSRISQIHSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
ALFKLKNLLEKVIQ
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612