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The map label for this gene is cheD

Identifier: 16078709

GI number: 16078709

Start: 1715970

End: 1716470

Strand: Direct

Name: cheD

Synonym: BSU16460

Alternate gene names: 16078709

Gene position: 1715970-1716470 (Clockwise)

Preceding gene: 16078708

Following gene: 16078710

Centisome position: 40.71

GC content: 45.31

Gene sequence:

>501_bases
ATGAGTACAACTGAGGCTGTTGTTATAAAGGTTGGGATTGCTGACGTGAAGATCGCCCGCTTCCCGGATACCATCCGGAC
CTCTGGTTTGGGCTCATGTGTGGGGCTGGTGCTTTATGATAAAGAAAAGCAAACGGCGGGTCTTGTTCATGTCATGCTTC
CGGATTCGACGTTATCGAAAACTGCCGAGCTCAACCGGGCTAAGTACGCTGACACCGCCGTACAGACCACGATTGATATG
CTGATAGAAGCGGGATGCCGGAAATTTGCATTAAAAGCAAAGCTGGCCGGCGGATCAGAGATGTTTAAATTTAAATCGAC
AAATGATTTGATGAAGATCGGACCGAGAAATGTATTAGCGATAAAAGAACAGCTGTCTTTATTTAATATTCCTATTATTA
GTGAAGATACGGGCGGCTCAAGCGGCCGGACGATAGAATTTGAACCGAAGTCCTGCATGCTGCATATTCGAACTGTTAAA
CAAGGTGAAAAAACGATTTAA

Upstream 100 bases:

>100_bases
TTTTTGACCAAAGCCATCAGCAGGAGCTGAAAGCGCATATGTTTATGCTGCCGGACTATGATTCATTTGAAAAGCTCTTT
GTCGCCTTAGGTGCATCATT

Downstream 100 bases:

>100_bases
TTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAG
ATCCTAAAGCCGGTGACGAC

Product: sequence specific deamidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSTTEAVVIKVGIADVKIARFPDTIRTSGLGSCVGLVLYDKEKQTAGLVHVMLPDSTLSKTAELNRAKYADTAVQTTIDM
LIEAGCRKFALKAKLAGGSEMFKFKSTNDLMKIGPRNVLAIKEQLSLFNIPIISEDTGGSSGRTIEFEPKSCMLHIRTVK
QGEKTI

Sequences:

>Translated_166_residues
MSTTEAVVIKVGIADVKIARFPDTIRTSGLGSCVGLVLYDKEKQTAGLVHVMLPDSTLSKTAELNRAKYADTAVQTTIDM
LIEAGCRKFALKAKLAGGSEMFKFKSTNDLMKIGPRNVLAIKEQLSLFNIPIISEDTGGSSGRTIEFEPKSCMLHIRTVK
QGEKTI
>Mature_165_residues
STTEAVVIKVGIADVKIARFPDTIRTSGLGSCVGLVLYDKEKQTAGLVHVMLPDSTLSKTAELNRAKYADTAVQTTIDML
IEAGCRKFALKAKLAGGSEMFKFKSTNDLMKIGPRNVLAIKEQLSLFNIPIISEDTGGSSGRTIEFEPKSCMLHIRTVKQ
GEKTI

Specific function: Deamidates 'Gln-593' and 'Gln-594' of the chemoreceptor McpA. In addition, deamidates other chemoreceptors, including McpB and McpC. CheD-mediated MCP (methyl-accepting chemotaxis proteins) deamidation is required for productive communication of the confo

COG id: COG1871

COG function: function code NT; Chemotaxis protein; stimulates methylation of MCP proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CheD family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHED_BACSU (P40404)

Other databases:

- EMBL:   M20144
- EMBL:   AL009126
- PIR:   B55216
- RefSeq:   NP_389528.1
- ProteinModelPortal:   P40404
- SMR:   P40404
- IntAct:   P40404
- EnsemblBacteria:   EBBACT00000002995
- GeneID:   939959
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16460
- NMPDR:   fig|224308.1.peg.1649
- GenoList:   BSU16460
- GeneTree:   EBGT00050000001785
- HOGENOM:   HBG531149
- OMA:   IGMIHIM
- ProtClustDB:   CLSK2392229
- BioCyc:   BSUB:BSU16460-MONOMER
- BRENDA:   3.5.1.44
- GO:   GO:0006935
- HAMAP:   MF_01440
- InterPro:   IPR005659

Pfam domain/function: PF03975 CheD

EC number: =3.5.1.44

Molecular weight: Translated: 18008; Mature: 17877

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTEAVVIKVGIADVKIARFPDTIRTSGLGSCVGLVLYDKEKQTAGLVHVMLPDSTLSK
CCCCCEEEEEEECCEEEEECCCCHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCHHHH
TAELNRAKYADTAVQTTIDMLIEAGCRKFALKAKLAGGSEMFKFKSTNDLMKIGPRNVLA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCEEEECCCCCHHHCCCCCEEE
IKEQLSLFNIPIISEDTGGSSGRTIEFEPKSCMLHIRTVKQGEKTI
HHHHHHEECCEEEECCCCCCCCCEEEECCHHEEEEEEEECCCCCCC
>Mature Secondary Structure 
STTEAVVIKVGIADVKIARFPDTIRTSGLGSCVGLVLYDKEKQTAGLVHVMLPDSTLSK
CCCCEEEEEEECCEEEEECCCCHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCHHHH
TAELNRAKYADTAVQTTIDMLIEAGCRKFALKAKLAGGSEMFKFKSTNDLMKIGPRNVLA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCEEEECCCCCHHHCCCCCEEE
IKEQLSLFNIPIISEDTGGSSGRTIEFEPKSCMLHIRTVKQGEKTI
HHHHHHEECCEEEECCCCCCCCCEEEECCHHEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377