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The map label for this gene is recG

Identifier: 16078650

GI number: 16078650

Start: 1659810

End: 1661858

Strand: Direct

Name: recG

Synonym: BSU15870

Alternate gene names: 16078650

Gene position: 1659810-1661858 (Clockwise)

Preceding gene: 16078649

Following gene: 16078651

Centisome position: 39.37

GC content: 45.39

Gene sequence:

>2049_bases
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGAAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCATGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGTTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTATTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAT
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGATCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTAAATGTTCCGAATGCAACAATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Upstream 100 bases:

>100_bases
ATGCCAACTGCACTTAGAGAAACAGGCCAGGGCGGTTTAGCGGCAACACCGACAGGAAGAGAATTAGAGAAAAAAATTTT
CGGAGGAGCGCTAGGTTCAC

Downstream 100 bases:

>100_bases
GTATCAGAAGTTTTTGGTGTTCAGCCAGAAACAATCGTTGCATTCCAATCTATGAAATTATATACTACTATTAGTACCTA
GTCTTAATTGTCCGGATGGT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Sequences:

>Translated_682_residues
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS
>Mature_682_residues
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367254, Length=658, Percent_Identity=39.209726443769, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1787357, Length=403, Percent_Identity=36.2282878411911, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECG_BACSU (O34942)

Other databases:

- EMBL:   Y13937
- EMBL:   AL009126
- PIR:   H69879
- RefSeq:   NP_389469.1
- ProteinModelPortal:   O34942
- SMR:   O34942
- EnsemblBacteria:   EBBACT00000002888
- GeneID:   936730
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15870
- NMPDR:   fig|224308.1.peg.1589
- GenoList:   BSU15870
- GeneTree:   EBGT00050000000887
- HOGENOM:   HBG747311
- OMA:   ETYWVKH
- PhylomeDB:   O34942
- ProtClustDB:   PRK10917
- BioCyc:   BSUB:BSU15870-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00643

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; SSF50249 Nucleic_acid_OB

EC number: =3.6.4.12

Molecular weight: Translated: 78142; Mature: 78142

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVT
CCCCHHCCHHHCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCEEE
VEGKVHSEPSLTYYGKKRNRLTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDK
EECEECCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCH
HRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFIQQALTQYADSLPDPLPEKLR
HHHCEEHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
AILSGYQGALMVPTEILAEQHADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEI
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCHHHHHHHHHHHCCCE
DILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRNKGQDPDVLFMTATPIPRTLA
EEEECCHHHHHHHHCEEEEEEEEECCHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCEEE
ITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
EEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVG
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEC
VNVPNATIMVIYDADRFGLSQLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETN
CCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
DGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRALETARQDAANLVASDAFWKE
CCCEECCCCEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEYAVLRDELLKSGVMDGEKLS
CCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVT
CCCCHHCCHHHCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCEEE
VEGKVHSEPSLTYYGKKRNRLTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDK
EECEECCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCH
HRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFIQQALTQYADSLPDPLPEKLR
HHHCEEHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
AILSGYQGALMVPTEILAEQHADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEI
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCHHHHHHHHHHHCCCE
DILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRNKGQDPDVLFMTATPIPRTLA
EEEECCHHHHHHHHCEEEEEEEEECCHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCEEE
ITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
EEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVG
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEC
VNVPNATIMVIYDADRFGLSQLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETN
CCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
DGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRALETARQDAANLVASDAFWKE
CCCEECCCCEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEYAVLRDELLKSGVMDGEKLS
CCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377