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The map label for this gene is thiN

Identifier: 16078643

GI number: 16078643

Start: 1654730

End: 1655374

Strand: Direct

Name: thiN

Synonym: BSU15800

Alternate gene names: 16078643

Gene position: 1654730-1655374 (Clockwise)

Preceding gene: 16078642

Following gene: 16078644

Centisome position: 39.25

GC content: 40.93

Gene sequence:

>645_bases
ATGAAAACAATTAATATCGTTGCGGGAGGCCCGAAAAATCTCATTCCCGATCTAACCGGCTATACGGATGAACACACGCT
TTGGATCGGTGTTGACAAAGGCACCGTCACTCTCTTAGATGCCGGGATCATTCCTGTTGAAGCCTTCGGAGATTTTGACA
GCATAACGGAGCAAGAACGCCGGCGAATAGAAAAAGCCGCTCCCGCCCTTCATGTGTATCAAGCAGAAAAAGATCAAACA
GATTTAGACCTCGCCCTTGATTGGGCGCTGGAAAAGCAGCCGGATATTATTCAGATTTTCGGCATTACAGGCGGCAGAGC
TGATCATTTTTTAGGAAACATTCAGCTTCTGTATAAAGGTGTAAAAACGAACATAAAAATTAGGCTGATAGACAAACAAA
ATCATATTCAAATGTTCCCTCCTGGTGAATATGATATTGAGAAGGATGAAAATAAGCGATATATCTCCTTCATACCGTTT
TCCGAAGACATACATGAGCTGACCCTGACCGGTTTTAAATATCCTCTAAATAATTGTCATATTACGCTCGGTTCAACACT
ATGTATTAGTAACGAACTCATTCATTCACGAGGTACTTTTTCGTTTGCAAAAGGCATATTAATAATGATAAGAAGCACGG
ATTAA

Upstream 100 bases:

>100_bases
AATTTCTGAAATCAGAGGAAGTAAATAAGCAGCTTTAGAAAAGAGCTGGGCATACAATAGCCTAAGCTCTTTTTCCTGTA
TGCAGAGAAAGGAATCAGAG

Downstream 100 bases:

>100_bases
GCATGTGCTTTCATTCAATTGCTGAAAACAATGAACAAGGCGGTTACGGGACGTTATAGGAGGGGACAAAAATGAAATTT
TACACCATTAAATTGCCGAA

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKTINIVAGGPKNLIPDLTGYTDEHTLWIGVDKGTVTLLDAGIIPVEAFGDFDSITEQERRRIEKAAPALHVYQAEKDQT
DLDLALDWALEKQPDIIQIFGITGGRADHFLGNIQLLYKGVKTNIKIRLIDKQNHIQMFPPGEYDIEKDENKRYISFIPF
SEDIHELTLTGFKYPLNNCHITLGSTLCISNELIHSRGTFSFAKGILIMIRSTD

Sequences:

>Translated_214_residues
MKTINIVAGGPKNLIPDLTGYTDEHTLWIGVDKGTVTLLDAGIIPVEAFGDFDSITEQERRRIEKAAPALHVYQAEKDQT
DLDLALDWALEKQPDIIQIFGITGGRADHFLGNIQLLYKGVKTNIKIRLIDKQNHIQMFPPGEYDIEKDENKRYISFIPF
SEDIHELTLTGFKYPLNNCHITLGSTLCISNELIHSRGTFSFAKGILIMIRSTD
>Mature_214_residues
MKTINIVAGGPKNLIPDLTGYTDEHTLWIGVDKGTVTLLDAGIIPVEAFGDFDSITEQERRRIEKAAPALHVYQAEKDQT
DLDLALDWALEKQPDIIQIFGITGGRADHFLGNIQLLYKGVKTNIKIRLIDKQNHIQMFPPGEYDIEKDENKRYISFIPF
SEDIHELTLTGFKYPLNNCHITLGSTLCISNELIHSRGTFSFAKGILIMIRSTD

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family

Homologues:

Organism=Drosophila melanogaster, GI28571654, Length=177, Percent_Identity=28.8135593220339, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIN_BACSU (O34664)

Other databases:

- EMBL:   Y13937
- EMBL:   AL009126
- PIR:   C69879
- RefSeq:   NP_389462.1
- PDB:   3LM8
- PDBsum:   3LM8
- ProteinModelPortal:   O34664
- SMR:   O34664
- EnsemblBacteria:   EBBACT00000002977
- GeneID:   937714
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15800
- NMPDR:   fig|224308.1.peg.1582
- GenoList:   BSU15800
- GeneTree:   EBGT00050000001518
- HOGENOM:   HBG615989
- OMA:   QVTIFGA
- ProtClustDB:   CLSK887291
- BioCyc:   BSUB:BSU15800-MONOMER
- BRENDA:   2.7.6.2
- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371
- Gene3D:   G3DSA:3.40.50.10240
- TIGRFAMs:   TIGR01378

Pfam domain/function: PF04263 TPK_catalytic; SSF63862 TPK_B1_bd; SSF63999 TPK_catalytic

EC number: =2.7.6.2

Molecular weight: Translated: 24099; Mature: 24099

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTINIVAGGPKNLIPDLTGYTDEHTLWIGVDKGTVTLLDAGIIPVEAFGDFDSITEQER
CCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHH
RRIEKAAPALHVYQAEKDQTDLDLALDWALEKQPDIIQIFGITGGRADHFLGNIQLLYKG
HHHHHHCCEEEEEECCCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHCEEEEEEC
VKTNIKIRLIDKQNHIQMFPPGEYDIEKDENKRYISFIPFSEDIHELTLTGFKYPLNNCH
CCCCEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCHHEEEECEECCCCCEE
ITLGSTLCISNELIHSRGTFSFAKGILIMIRSTD
EEECCEEEECCHHHHCCCCHHCCCEEEEEEEECC
>Mature Secondary Structure
MKTINIVAGGPKNLIPDLTGYTDEHTLWIGVDKGTVTLLDAGIIPVEAFGDFDSITEQER
CCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHH
RRIEKAAPALHVYQAEKDQTDLDLALDWALEKQPDIIQIFGITGGRADHFLGNIQLLYKG
HHHHHHCCEEEEEECCCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHCEEEEEEC
VKTNIKIRLIDKQNHIQMFPPGEYDIEKDENKRYISFIPFSEDIHELTLTGFKYPLNNCH
CCCCEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCHHEEEECEECCCCCEE
ITLGSTLCISNELIHSRGTFSFAKGILIMIRSTD
EEECCEEEECCHHHHCCCCHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377