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The map label for this gene is murD

Identifier: 16078584

GI number: 16078584

Start: 1588901

End: 1590256

Strand: Direct

Name: murD

Synonym: BSU15200

Alternate gene names: 16078584

Gene position: 1588901-1590256 (Clockwise)

Preceding gene: 255767348

Following gene: 16078585

Centisome position: 37.69

GC content: 45.06

Gene sequence:

>1356_bases
GTGGAAAATGATCAATTTTTACAGAAACAGCATTTTTTAATCCTTGGGCTTGCGAAAAGCGGATATGCGGCGGCCTCCAT
TCTTCATGAAAAAGGCATTTATGTTGCAGTCAATGATCAAAAGCCTTTTGAAGAAAATGAACCGGCACAAAAGCTTTCTG
AAAAAGGAATCGAAGTTGTTTGCGGAGAGCATCCAGTTTCTCTGTTCGATCAGCATCAAATCACGATTCTGATCAAAAAC
CCGGGAATTCCTTATGAAAATATCATGGTTCAGGAAGCAGAGAAACGAGGTATTCCGGTTTGGACGGAAATTGAGCTCGC
TTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAA
TGCTCAAGGCTGACTCGCAAAAAGCACTGATTGCCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGAT
GGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGCCTGAT
TTTAAATGTATTTGACGCCCATCTGGATTATCACCATACGCGCGAAAACTATGAAAAAGCGAAACAGAAAGTGTACCTTC
ATCAGACTGCTTCAGATAAAGCTATTGTCAACCAGGATGATGAAACCGTCGTGCGTCTTGCTGAAGCCGGAAAAGCCGAA
ATCGTCCCATTTTCAGTCAGCAAAACGCTTGAACAGGGCGCATATGTAAAAGACAGTATGATTATGTTTAACGGCGAAGC
CATTCTTCCTCTGGAAGAGGTTGTACTGCCCGGTGCTCATAACCTGGAGAACATTTTAGCAGCTATTGCAGTTGTGAAAA
CGGCAGGAGCATCCAATGAGGCGGTTAAAAAGGTGCTGACAAGCTTTACTGGTGTGAAGCACCGCCTCCAATATGTCACA
ACTGTGAATGGCCGCAAATTTTATAACGACAGTAAAGCAACAAACATTCTGGCGACAAGCAAGGCGCTTTCAGCTTTTGA
TAAGCCGGTCATTCTGCTTGCGGGCGGACTGGATCGCGGAAATGGTTTTGATGACTTGAAACCTTATATGAAGCATGTCA
AAGCAGTGCTTACATTTGGCCAGACGGCGCCAAAACTCGAGAAACTCGGCAATGAACTGGGAATACAACATGTCAAACGT
GTCGATAATGTTGAACAAGCAGTATCTGCGGCGTTTGCGCTCTCAAATGAAGGAGATGTCATCCTGCTGTCACCCGCGTG
TGCGAGCTGGGATCAATTCAAAACATTTGAAGAACGCGGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAA

Upstream 100 bases:

>100_bases
TGAGCTTGTCGGCTGGTCTGAATGGAGAGTAGTCGTGACGTTTTGGGCTGCGGGACTTTTGCTTGCCGTTTTAGGAATTT
ACATCGAGGTGTGGTTATAA

Downstream 100 bases:

>100_bases
GGGCTTGTCTTGAAGTAAATCGGCAGCCCTAATGACTTTAAGCTTGGGGTGTCGATTGCTTTGACTACTAAAAAAACATC
GCCTGATTTGTTATTGGTCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MENDQFLQKQHFLILGLAKSGYAAASILHEKGIYVAVNDQKPFEENEPAQKLSEKGIEVVCGEHPVSLFDQHQITILIKN
PGIPYENIMVQEAEKRGIPVWTEIELAYYLTSAKFIGITGSNGKTTTTTLIYEMLKADSQKALIAGNIGTVASEVAYHAD
GDEWIVTELSSFQLMGTHAFRPEISLILNVFDAHLDYHHTRENYEKAKQKVYLHQTASDKAIVNQDDETVVRLAEAGKAE
IVPFSVSKTLEQGAYVKDSMIMFNGEAILPLEEVVLPGAHNLENILAAIAVVKTAGASNEAVKKVLTSFTGVKHRLQYVT
TVNGRKFYNDSKATNILATSKALSAFDKPVILLAGGLDRGNGFDDLKPYMKHVKAVLTFGQTAPKLEKLGNELGIQHVKR
VDNVEQAVSAAFALSNEGDVILLSPACASWDQFKTFEERGDMFIDAVHMLK

Sequences:

>Translated_451_residues
MENDQFLQKQHFLILGLAKSGYAAASILHEKGIYVAVNDQKPFEENEPAQKLSEKGIEVVCGEHPVSLFDQHQITILIKN
PGIPYENIMVQEAEKRGIPVWTEIELAYYLTSAKFIGITGSNGKTTTTTLIYEMLKADSQKALIAGNIGTVASEVAYHAD
GDEWIVTELSSFQLMGTHAFRPEISLILNVFDAHLDYHHTRENYEKAKQKVYLHQTASDKAIVNQDDETVVRLAEAGKAE
IVPFSVSKTLEQGAYVKDSMIMFNGEAILPLEEVVLPGAHNLENILAAIAVVKTAGASNEAVKKVLTSFTGVKHRLQYVT
TVNGRKFYNDSKATNILATSKALSAFDKPVILLAGGLDRGNGFDDLKPYMKHVKAVLTFGQTAPKLEKLGNELGIQHVKR
VDNVEQAVSAAFALSNEGDVILLSPACASWDQFKTFEERGDMFIDAVHMLK
>Mature_451_residues
MENDQFLQKQHFLILGLAKSGYAAASILHEKGIYVAVNDQKPFEENEPAQKLSEKGIEVVCGEHPVSLFDQHQITILIKN
PGIPYENIMVQEAEKRGIPVWTEIELAYYLTSAKFIGITGSNGKTTTTTLIYEMLKADSQKALIAGNIGTVASEVAYHAD
GDEWIVTELSSFQLMGTHAFRPEISLILNVFDAHLDYHHTRENYEKAKQKVYLHQTASDKAIVNQDDETVVRLAEAGKAE
IVPFSVSKTLEQGAYVKDSMIMFNGEAILPLEEVVLPGAHNLENILAAIAVVKTAGASNEAVKKVLTSFTGVKHRLQYVT
TVNGRKFYNDSKATNILATSKALSAFDKPVILLAGGLDRGNGFDDLKPYMKHVKAVLTFGQTAPKLEKLGNELGIQHVKR
VDNVEQAVSAAFALSNEGDVILLSPACASWDQFKTFEERGDMFIDAVHMLK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=447, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BACSU (Q03522)

Other databases:

- EMBL:   Z15056
- EMBL:   AL009126
- EMBL:   X64258
- PIR:   D47691
- RefSeq:   NP_389403.1
- ProteinModelPortal:   Q03522
- SMR:   Q03522
- EnsemblBacteria:   EBBACT00000001305
- GeneID:   935950
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15200
- NMPDR:   fig|224308.1.peg.1522
- GenoList:   BSU15200
- GeneTree:   EBGT00070000032130
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q03522
- ProtClustDB:   PRK02472
- BioCyc:   BSUB:BSU15200-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49651; Mature: 49651

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDQFLQKQHFLILGLAKSGYAAASILHEKGIYVAVNDQKPFEENEPAQKLSEKGIEVV
CCCHHHHHHCCEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCCEEE
CGEHPVSLFDQHQITILIKNPGIPYENIMVQEAEKRGIPVWTEIELAYYLTSAKFIGITG
ECCCCCCCCCCCEEEEEEECCCCCHHHHHEEHHHHCCCCEEEEEEEEEEEECCEEEEEEC
SNGKTTTTTLIYEMLKADSQKALIAGNIGTVASEVAYHADGDEWIVTELSSFQLMGTHAF
CCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCEEEEECCCEEEEECCCC
RPEISLILNVFDAHLDYHHTRENYEKAKQKVYLHQTASDKAIVNQDDETVVRLAEAGKAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCHHHHHHHHHCCCCE
IVPFSVSKTLEQGAYVKDSMIMFNGEAILPLEEVVLPGAHNLENILAAIAVVKTAGASNE
EEEEHHHHHHHCCCEEECCEEEECCCEECCHHHHHCCCCCCHHHHHHHHHHHHCCCCCHH
AVKKVLTSFTGVKHRLQYVTTVNGRKFYNDSKATNILATSKALSAFDKPVILLAGGLDRG
HHHHHHHHHHHHHHHEEEEEEECCCEEECCCCCCEEEEHHHHHHHCCCCEEEEECCCCCC
NGFDDLKPYMKHVKAVLTFGQTAPKLEKLGNELGIQHVKRVDNVEQAVSAAFALSNEGDV
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCE
ILLSPACASWDQFKTFEERGDMFIDAVHMLK
EEECCCCCCHHHHHHHHHHCCEEEHHHHHCC
>Mature Secondary Structure
MENDQFLQKQHFLILGLAKSGYAAASILHEKGIYVAVNDQKPFEENEPAQKLSEKGIEVV
CCCHHHHHHCCEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCCEEE
CGEHPVSLFDQHQITILIKNPGIPYENIMVQEAEKRGIPVWTEIELAYYLTSAKFIGITG
ECCCCCCCCCCCEEEEEEECCCCCHHHHHEEHHHHCCCCEEEEEEEEEEEECCEEEEEEC
SNGKTTTTTLIYEMLKADSQKALIAGNIGTVASEVAYHADGDEWIVTELSSFQLMGTHAF
CCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCEEEEECCCEEEEECCCC
RPEISLILNVFDAHLDYHHTRENYEKAKQKVYLHQTASDKAIVNQDDETVVRLAEAGKAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCHHHHHHHHHCCCCE
IVPFSVSKTLEQGAYVKDSMIMFNGEAILPLEEVVLPGAHNLENILAAIAVVKTAGASNE
EEEEHHHHHHHCCCEEECCEEEECCCEECCHHHHHCCCCCCHHHHHHHHHHHHCCCCCHH
AVKKVLTSFTGVKHRLQYVTTVNGRKFYNDSKATNILATSKALSAFDKPVILLAGGLDRG
HHHHHHHHHHHHHHHEEEEEEECCCEEECCCCCCEEEEHHHHHHHCCCCEEEEECCCCCC
NGFDDLKPYMKHVKAVLTFGQTAPKLEKLGNELGIQHVKRVDNVEQAVSAAFALSNEGDV
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCE
ILLSPACASWDQFKTFEERGDMFIDAVHMLK
EEECCCCCCHHHHHHHHHHCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436954; 9384377; 1391053