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The map label for this gene is murE

Identifier: 16078582

GI number: 16078582

Start: 1586330

End: 1587814

Strand: Direct

Name: murE

Synonym: BSU15180

Alternate gene names: 16078582

Gene position: 1586330-1587814 (Clockwise)

Preceding gene: 255767347

Following gene: 255767348

Centisome position: 37.63

GC content: 46.13

Gene sequence:

>1485_bases
ATGAAACTTACAAAGCTGCTTACATACTTAACAACCGAACCTTCTGTAAACGACTCACAAGACCCTGAAATAACTTCAAT
TGAGATGGACTCCAGGGAAGTAAAGAAAGGGAGTCTTTTTGTATGTGTAAAAGGGTATACGGTGGATGGCCACGATTTTG
CGCAAAAAGCAGTAGAAAACGGTGCAGCGGCTATTGTGGCAGAAAGAGAGGTCGATGTGAATGTTCCTGTTATTATTGTC
CGGCAGTCACTTCGCGCGCTCAGCGTTTTGTCTGATGCGTTTTACGGACAGCCGACGAAGAAGCTTCAGCTGATTGGGAT
AACAGGGACAAACGGCAAAACATCAACGACCCACATGGTCGATGAGATTCTGAAAAAAGCGGGAAAACGCACAGGCTTGA
TCGGTACAATGTACATGAAAATCGGAGACGAAACACTTCCTGTTAAAAACACGACTCCGGAAAGCGTCACGCTGCAGAAA
ACGTTTAAGAAAATGAATGACAAGCACGTTGACACTGCAATCATGGAGGTTTCTTCCCACGCTTTGTCTTTAGGACGCGT
CCATGGCTGTGATTATGATATTGCCGTGTTTACGAATTTAACACAGGACCATCTGGACTATCATAAAACGATGGATGAGT
ACCGGCATGCAAAAAGCTTGCTCTTTTCCCAACTTGGCGGGGCTTTCAACCATGAACATCCAAAGCGTGCCGTCCTGAAT
GCTGACGATGAGGCGAGCGCTTATTTTGAAAAGGTAACAGCTGCGCATATCTCTACATACGGAATTAAAAACGATGCTGA
TGTGATGGCGAAGAACATCAGTATCACTGCACAGGGCACAAGCTTTGATCTCGTGACAAACAAAGGCACGAAGCACATCA
CGATGTCTCTCGTCGGACAATTTAATGTGTATAACGTGCTGGCCGCTGTTGCGACATGTATTGCAGCAGGCATACCGTTT
GAAATCATCACTGAGGCTGTTGAAGAATTGCATGGTGTAAGAGGCCGTTTTGAATTGGTCAATCAGCAGCAGGAGTTTCC
TGTCATTGTAGACTACGCGCACACACCGGACAGCCTTGAAAATGTGCTGGAGACATGCAGAGACATGACGGAAGGAAAGC
TATTTGTCGTTGTCGGCTGCGGAGGAGACAGAGATAAGACGAAGCGCCCGAAAATGGCTAAAATTGCTGTGGAGCTGGCT
GACGAGCCGATTTTCACGTCAGATAACCCGAGAAGCGAAGATCCTCGCGCGATTTTAAGAGATATGGAAGCCGGAGTAGA
GAACGCATACTATCACAGTATTGCAAACCGCGAGCAGGCGATCTTTTTCGCAATTGCCAATGCCAAAAAAGGCGACGTTG
TCCTCATTGCCGGAAAAGGACATGAAACCTATCAGCAGATCGGAAATGAAACTTTCGATTTTGATGATGCTGAAGTAGCG
GCGAGAGCCATTGTTGAACTCAATAAGAATAAGACGAATTCATAG

Upstream 100 bases:

>100_bases
ACGCTAAAATATGAACTGTATGCTTAAAATCATTACCACATCATTTGATGTGACTTCAATGATAAAAAGAACGAATTAGA
ATGTTGAGAGGTTTGATACG

Downstream 100 bases:

>100_bases
GAAAGAGATGTTACTGAATAATGGAGCTTGAAAAGATTTTTCATATGGAAAAAAATCATGAATATGATAACTACGTATGT
CGTTTGAAAGAAACAGGAGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKLTKLLTYLTTEPSVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVENGAAAIVAEREVDVNVPVIIV
RQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKTSTTHMVDEILKKAGKRTGLIGTMYMKIGDETLPVKNTTPESVTLQK
TFKKMNDKHVDTAIMEVSSHALSLGRVHGCDYDIAVFTNLTQDHLDYHKTMDEYRHAKSLLFSQLGGAFNHEHPKRAVLN
ADDEASAYFEKVTAAHISTYGIKNDADVMAKNISITAQGTSFDLVTNKGTKHITMSLVGQFNVYNVLAAVATCIAAGIPF
EIITEAVEELHGVRGRFELVNQQQEFPVIVDYAHTPDSLENVLETCRDMTEGKLFVVVGCGGDRDKTKRPKMAKIAVELA
DEPIFTSDNPRSEDPRAILRDMEAGVENAYYHSIANREQAIFFAIANAKKGDVVLIAGKGHETYQQIGNETFDFDDAEVA
ARAIVELNKNKTNS

Sequences:

>Translated_494_residues
MKLTKLLTYLTTEPSVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVENGAAAIVAEREVDVNVPVIIV
RQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKTSTTHMVDEILKKAGKRTGLIGTMYMKIGDETLPVKNTTPESVTLQK
TFKKMNDKHVDTAIMEVSSHALSLGRVHGCDYDIAVFTNLTQDHLDYHKTMDEYRHAKSLLFSQLGGAFNHEHPKRAVLN
ADDEASAYFEKVTAAHISTYGIKNDADVMAKNISITAQGTSFDLVTNKGTKHITMSLVGQFNVYNVLAAVATCIAAGIPF
EIITEAVEELHGVRGRFELVNQQQEFPVIVDYAHTPDSLENVLETCRDMTEGKLFVVVGCGGDRDKTKRPKMAKIAVELA
DEPIFTSDNPRSEDPRAILRDMEAGVENAYYHSIANREQAIFFAIANAKKGDVVLIAGKGHETYQQIGNETFDFDDAEVA
ARAIVELNKNKTNS
>Mature_494_residues
MKLTKLLTYLTTEPSVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVENGAAAIVAEREVDVNVPVIIV
RQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKTSTTHMVDEILKKAGKRTGLIGTMYMKIGDETLPVKNTTPESVTLQK
TFKKMNDKHVDTAIMEVSSHALSLGRVHGCDYDIAVFTNLTQDHLDYHKTMDEYRHAKSLLFSQLGGAFNHEHPKRAVLN
ADDEASAYFEKVTAAHISTYGIKNDADVMAKNISITAQGTSFDLVTNKGTKHITMSLVGQFNVYNVLAAVATCIAAGIPF
EIITEAVEELHGVRGRFELVNQQQEFPVIVDYAHTPDSLENVLETCRDMTEGKLFVVVGCGGDRDKTKRPKMAKIAVELA
DEPIFTSDNPRSEDPRAILRDMEAGVENAYYHSIANREQAIFFAIANAKKGDVVLIAGKGHETYQQIGNETFDFDDAEVA
ARAIVELNKNKTNS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=490, Percent_Identity=38.1632653061225, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1786274, Length=374, Percent_Identity=26.7379679144385, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_BACSU (Q03523)

Other databases:

- EMBL:   Z15056
- EMBL:   AL009126
- EMBL:   Z25865
- PIR:   B47691
- RefSeq:   NP_389401.1
- ProteinModelPortal:   Q03523
- SMR:   Q03523
- EnsemblBacteria:   EBBACT00000002053
- GeneID:   937567
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15180
- NMPDR:   fig|224308.1.peg.1520
- GenoList:   BSU15180
- GeneTree:   EBGT00050000000629
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- PhylomeDB:   Q03523
- ProtClustDB:   PRK00139
- BioCyc:   BSUB:BSU15180-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54326; Mature: 54326

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: BINDING 31-31 BINDING 150-150 BINDING 178-178 BINDING 186-186 BINDING 384-384 BINDING 458-458 BINDING 462-462

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKLLTYLTTEPSVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVEN
CCHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHCCCCCEEEEEECEEECCHHHHHHHHHC
GAAAIVAEREVDVNVPVIIVRQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKTSTTHMV
CCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHH
DEILKKAGKRTGLIGTMYMKIGDETLPVKNTTPESVTLQKTFKKMNDKHVDTAIMEVSSH
HHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
ALSLGRVHGCDYDIAVFTNLTQDHLDYHKTMDEYRHAKSLLFSQLGGAFNHEHPKRAVLN
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
ADDEASAYFEKVTAAHISTYGIKNDADVMAKNISITAQGTSFDLVTNKGTKHITMSLVGQ
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCEEEEEECCCCCEEEEEEHHC
FNVYNVLAAVATCIAAGIPFEIITEAVEELHGVRGRFELVNQQQEFPVIVDYAHTPDSLE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCHHHH
NVLETCRDMTEGKLFVVVGCGGDRDKTKRPKMAKIAVELADEPIFTSDNPRSEDPRAILR
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCHHHHHH
DMEAGVENAYYHSIANREQAIFFAIANAKKGDVVLIAGKGHETYQQIGNETFDFDDAEVA
HHHHCHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHH
ARAIVELNKNKTNS
HHHHHHHCCCCCCC
>Mature Secondary Structure
MKLTKLLTYLTTEPSVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVEN
CCHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHCCCCCEEEEEECEEECCHHHHHHHHHC
GAAAIVAEREVDVNVPVIIVRQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKTSTTHMV
CCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHH
DEILKKAGKRTGLIGTMYMKIGDETLPVKNTTPESVTLQKTFKKMNDKHVDTAIMEVSSH
HHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
ALSLGRVHGCDYDIAVFTNLTQDHLDYHKTMDEYRHAKSLLFSQLGGAFNHEHPKRAVLN
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
ADDEASAYFEKVTAAHISTYGIKNDADVMAKNISITAQGTSFDLVTNKGTKHITMSLVGQ
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCEEEEEECCCCCEEEEEEHHC
FNVYNVLAAVATCIAAGIPFEIITEAVEELHGVRGRFELVNQQQEFPVIVDYAHTPDSLE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCHHHH
NVLETCRDMTEGKLFVVVGCGGDRDKTKRPKMAKIAVELADEPIFTSDNPRSEDPRAILR
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCHHHHHH
DMEAGVENAYYHSIANREQAIFFAIANAKKGDVVLIAGKGHETYQQIGNETFDFDDAEVA
HHHHCHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHH
ARAIVELNKNKTNS
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436954; 9384377; 8289242