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The map label for this gene is nprE

Identifier: 16078534

GI number: 16078534

Start: 1540036

End: 1541601

Strand: Reverse

Name: nprE

Synonym: BSU14700

Alternate gene names: 16078534

Gene position: 1541601-1540036 (Counterclockwise)

Preceding gene: 16078539

Following gene: 16078533

Centisome position: 36.57

GC content: 44.83

Gene sequence:

>1566_bases
GTGGGTTTAGGTAAGAAATTGTCTGTTGCTGTCGCTGCTTCGTTTATGAGTTTATCAATCAGCCTGCCAGGTGTTCAGGC
TGCTGAAGGTCATCAGCTTAAAGAGAATCAAACAAATTTCCTCTCCAAAAACGCGATTGCGCAATCAGAACTCTCTGCAC
CAAATGACAAGGCTGTCAAGCAGTTTTTGAAAAAGAACAGCAACATTTTTAAAGGTGACCCTTCCAAAAGGCTGAAGCTT
GTTGAAAGCACGACTGATGCCCTTGGATACAAGCACTTTCGATATGCGCCTGTCGTTAACGGAGTGCCAATTAAAGATTC
GCAAGTGATCGTTCACGTCGATAAATCCGATAATGTCTATGCGGTCAATGGTGAATTACACAATCAATCTGCTGCAAAAA
CAGATAACAGCCAAAAAGTCTCTTCTGAAAAAGCGCTGGCACTCGCTTTCAAAGCTATCGGCAAATCACCAGACGCTGTT
TCTAACGGAGCGGCCAAAAACAGCAATAAAGCCGAATTAAAAGCGATAGAAACAAAAGACGGCAGCTATCGTCTTGCTTA
CGACGTGACGATTCGCTATGTCGAGCCTGAACCTGCAAACTGGGAAGTCTTAGTTGACGCCGAAACAGGCAGCATTTTAA
AACAGCAAAATAAAGTAGAACATGCCGCCGCCACTGGAAGCGGAACAACGCTAAAGGGCGCAACTGTTCCTTTGAACATC
TCTTATGAAGGCGGAAAATATGTTCTAAGAGATCTTTCAAAACCAACAGGCACCCAAATCATCACATATGATTTGCAAAA
CAGACAAAGCCGCCTTCCGGGCACGCTTGTCTCAAGCACAACGAAAACATTTACATCTTCATCACAGCGGGCAGCCGTTG
ACGCACACTATAACCTCGGTAAAGTGTACGATTATTTTTATTCAAACTTTAAACGAAACAGCTATGATAACAAAGGCAGT
AAAATCGTTTCTTCCGTTCACTACGGCACTCAATACAATAACGCTGCATGGACAGGAGACCAGATGATTTACGGTGATGG
CGACGGTTCATTCTTCTCTCCGCTTTCCGGCTCATTAGATGTGACAGCGCATGAAATGACACATGGCGTCACCCAAGAAA
CAGCCAACTTGATTTATGAAAATCAGCCAGGTGCATTAAACGAGTCTTTCTCTGACGTATTCGGGTATTTTAACGATACA
GAAGACTGGGACATCGGTGAAGACATTACGGTCAGCCAGCCTGCTCTTCGCAGCCTGTCCAACCCTACAAAATACAACCA
GCCTGACAATTACGCCAATTACCGAAACCTTCCAAACACAGATGAAGGCGATTATGGCGGTGTACACACAAACAGCGGAA
TTCCAAACAAAGCCGCTTACAACACCATCACAAAACTTGGTGTATCTAAATCACAGCAAATCTATTACCGTGCGTTAACA
ACGTACCTCACGCCTTCTTCCACGTTCAAAGATGCCAAGGCAGCTCTCATTCAGTCTGCCCGTGACCTCTACGGCTCAAC
TGATGCCGCTAAAGTTGAAGCAGCCTGGAATGCTGTTGGATTGTAA

Upstream 100 bases:

>100_bases
CAATTTTGTCTAGTTATGTTAGTTTTTGTTGAGTATTCCAGAATGCTAGTTTAATATAACAATATAAAGTTTTCAGTATT
TTCAAAAAGGGGGATTTATT

Downstream 100 bases:

>100_bases
TATTAGGAAAAGCCTGAGATCCCTCAGGCTTTTATTGTTACATATCTTGATTTCTCTCTCAGCTGAAACGACGAAAAGAT
GCTGCCATGAGACAGAAAAC

Product: extracellular neutral metalloprotease

Products: NA

Alternate protein names: MCP 76; Neutral protease

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MGLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKNAIAQSELSAPNDKAVKQFLKKNSNIFKGDPSKRLKL
VESTTDALGYKHFRYAPVVNGVPIKDSQVIVHVDKSDNVYAVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAV
SNGAAKNSNKAELKAIETKDGSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNKVEHAAATGSGTTLKGATVPLNI
SYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGS
KIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDT
EDWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALT
TYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL

Sequences:

>Translated_521_residues
MGLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKNAIAQSELSAPNDKAVKQFLKKNSNIFKGDPSKRLKL
VESTTDALGYKHFRYAPVVNGVPIKDSQVIVHVDKSDNVYAVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAV
SNGAAKNSNKAELKAIETKDGSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNKVEHAAATGSGTTLKGATVPLNI
SYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGS
KIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDT
EDWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALT
TYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL
>Mature_520_residues
GLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKNAIAQSELSAPNDKAVKQFLKKNSNIFKGDPSKRLKLV
ESTTDALGYKHFRYAPVVNGVPIKDSQVIVHVDKSDNVYAVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAVS
NGAAKNSNKAELKAIETKDGSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNKVEHAAATGSGTTLKGATVPLNIS
YEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGSK
IVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDTE
DWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALTT
YLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL

Specific function: Extracellular zinc metalloprotease

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPRE_BACSA (P68735)

Other databases:

- EMBL:   D10773
- ProteinModelPortal:   P68735
- SMR:   P68735
- BRENDA:   3.4.24.28
- GO:   GO:0006508
- InterPro:   IPR005075
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096
- Gene3D:   G3DSA:3.10.170.10
- Gene3D:   G3DSA:1.10.390.10
- PRINTS:   PR00730

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C

EC number: =3.4.24.28

Molecular weight: Translated: 56522; Mature: 56391

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 365-365 ACT_SITE 449-449

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKNAIAQSELSAPNDKAVK
CCCCCHHHHHHHEEEHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
QFLKKNSNIFKGDPSKRLKLVESTTDALGYKHFRYAPVVNGVPIKDSQVIVHVDKSDNVY
HHHHCCCCEECCCCHHHEEEHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEECCCCCEE
AVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAVSNGAAKNSNKAELKAIETKD
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEEECC
GSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNKVEHAAATGSGTTLKGATVPLNI
CCEEEEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEE
SYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLG
EECCCHHHHHHCCCCCCCEEEEEECCCHHHCCCCHHHHCCHHHHCCCCCCEEEECCCCHH
KVYDYFYSNFKRNSYDNKGSKIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLD
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCE
VTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDTEDWDIGEDITVSQPALRSLS
EEHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCC
NPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALT
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCC
>Mature Secondary Structure 
GLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKNAIAQSELSAPNDKAVK
CCCCHHHHHHHEEEHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
QFLKKNSNIFKGDPSKRLKLVESTTDALGYKHFRYAPVVNGVPIKDSQVIVHVDKSDNVY
HHHHCCCCEECCCCHHHEEEHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEECCCCCEE
AVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAVSNGAAKNSNKAELKAIETKD
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEEECC
GSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNKVEHAAATGSGTTLKGATVPLNI
CCEEEEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEE
SYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLG
EECCCHHHHHHCCCCCCCEEEEEECCCHHHCCCCHHHHCCHHHHCCCCCCEEEECCCCHH
KVYDYFYSNFKRNSYDNKGSKIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLD
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCE
VTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDTEDWDIGEDITVSQPALRSLS
EEHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCC
NPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALT
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA