Click here to switch to the map view.

The map label for this gene is ykpB

Identifier: 16078508

GI number: 16078508

Start: 1514052

End: 1514963

Strand: Direct

Name: ykpB

Synonym: BSU14440

Alternate gene names: 16078508

Gene position: 1514052-1514963 (Clockwise)

Preceding gene: 16078507

Following gene: 16078512

Centisome position: 35.92

GC content: 45.29

Gene sequence:

>912_bases
ATGAAATTTTTGGTTGTCGGAGCAGGTGGAGTAGGCGGGTATATTGGCGGACGGCTTTCGGAGAAAGGAAATGATGTGAC
ATTTCTCGTGCGCCAAAAACGAGCTGAGCAGCTTAAAAAAACCGGGCTTGTCATCCATAGTGAAAAAGGGAATGTATCAT
TTCAGCCCGAACTAATCAGTGCCGGAGAAACAGGGCAATTTGATGTCGTTATCATTGCTTCTAAAGCATACTCGCTTGGT
CAAGTGATAGAGGATGTCAAACCATTTATCCATCAAGAATCTGTCATTATCCCTTTTTTAAATGGGTACCGCCACTATGA
GCAGCTATTTGCGGCATTTTCAAAAGAACAGGTGCTGGGCGGCCTGTGTTTTATAGAAAGTGCTTTAGACAACAAAGGAG
AAATTCATCATACGAGCGCATCGCATCGTTTTGTATTTGGAGAATGGAACGGCGAGCGTACGGAGCGGATAAGAGCGCTT
GAAGAGGCATTTTCAGGTGTGAAGGCTGAAGTCATCATTAGCGGGCATATCGAGAAGGACATTTGGAAAAAGTATCTCTT
TATTGCAGCGCAAGCGGGGATCACAACGTTATTTCAACGACCGCTTGGCCCAATCCTCGCCACAGAAGCCGGACGTCACA
CGGCCCAAACTCTTATTGGGGAAATTTGCACTGTTTTACGAAAAGAAGGTGTTCCGGCTGATCCGGCTCTTGAGGAAGAG
AGCTTTCGTACGATGACCAGCATGTCTTACCATATGAAGTCCTCCATGCTTCGGGATATGGAAAACGGCCAAACGACAGA
AGGCGACCACCTGCATGGATTTTTGATTGAAAAAGCAAAACGTTTATCTCTCGCTGCACCAGTATTAGAAACCGTTTATG
CGAATCTGCAAATGTATGAAGCAGAAAAATAA

Upstream 100 bases:

>100_bases
ATGCTGATGTGCAGAAAAAATTGACTGAACTATACGCCGAATAAAAAAGCAGAGATTTCTCTGCTTTTTTTGATACCTAA
ATGTGAAAGGAGATCACAAC

Downstream 100 bases:

>100_bases
AAAAAGGAGGCGGAAAAGCCTCCTTTTATTTACTTAAAAAGCCCAATTTCCGTTTCTGAAGATAGGCTCTCTTTTCCCGT
CTGCCGTAATTCCGTCAATA

Product: 2-dehydropantoate 2-reductase

Products: Nicotinamide adenine dinucleotide phosphate; (R)-Pantoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKFLVVGAGGVGGYIGGRLSEKGNDVTFLVRQKRAEQLKKTGLVIHSEKGNVSFQPELISAGETGQFDVVIIASKAYSLG
QVIEDVKPFIHQESVIIPFLNGYRHYEQLFAAFSKEQVLGGLCFIESALDNKGEIHHTSASHRFVFGEWNGERTERIRAL
EEAFSGVKAEVIISGHIEKDIWKKYLFIAAQAGITTLFQRPLGPILATEAGRHTAQTLIGEICTVLRKEGVPADPALEEE
SFRTMTSMSYHMKSSMLRDMENGQTTEGDHLHGFLIEKAKRLSLAAPVLETVYANLQMYEAEK

Sequences:

>Translated_303_residues
MKFLVVGAGGVGGYIGGRLSEKGNDVTFLVRQKRAEQLKKTGLVIHSEKGNVSFQPELISAGETGQFDVVIIASKAYSLG
QVIEDVKPFIHQESVIIPFLNGYRHYEQLFAAFSKEQVLGGLCFIESALDNKGEIHHTSASHRFVFGEWNGERTERIRAL
EEAFSGVKAEVIISGHIEKDIWKKYLFIAAQAGITTLFQRPLGPILATEAGRHTAQTLIGEICTVLRKEGVPADPALEEE
SFRTMTSMSYHMKSSMLRDMENGQTTEGDHLHGFLIEKAKRLSLAAPVLETVYANLQMYEAEK
>Mature_303_residues
MKFLVVGAGGVGGYIGGRLSEKGNDVTFLVRQKRAEQLKKTGLVIHSEKGNVSFQPELISAGETGQFDVVIIASKAYSLG
QVIEDVKPFIHQESVIIPFLNGYRHYEQLFAAFSKEQVLGGLCFIESALDNKGEIHHTSASHRFVFGEWNGERTERIRAL
EEAFSGVKAEVIISGHIEKDIWKKYLFIAAQAGITTLFQRPLGPILATEAGRHTAQTLIGEICTVLRKEGVPADPALEEE
SFRTMTSMSYHMKSSMLRDMENGQTTEGDHLHGFLIEKAKRLSLAAPVLETVYANLQMYEAEK

Specific function: Catalyzes The NADPH-Dependent Reduction Of Ketopantoate Into Pantoic Acid. [C]

COG id: COG1893

COG function: function code H; Ketopantoate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ketopantoate reductase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YKPB_BACSU (O31717)

Other databases:

- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   F69861
- RefSeq:   NP_389327.1
- ProteinModelPortal:   O31717
- SMR:   O31717
- EnsemblBacteria:   EBBACT00000003243
- GeneID:   938752
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14440
- NMPDR:   fig|224308.1.peg.1446
- GenoList:   BSU14440
- GeneTree:   EBGT00050000001655
- HOGENOM:   HBG668370
- OMA:   CKAFDLD
- ProtClustDB:   PRK12921
- BioCyc:   BSUB:BSU14440-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008927
- InterPro:   IPR003710
- InterPro:   IPR013752
- InterPro:   IPR013332
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR21708:SF21
- TIGRFAMs:   TIGR00745

Pfam domain/function: PF02558 ApbA; PF08546 ApbA_C; SSF48179 6DGDH_C_like

EC number: 1.1.1.169 [C]

Molecular weight: Translated: 33573; Mature: 33573

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: ACT_SITE 184-184 BINDING 101-101 BINDING 267-267

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLVVGAGGVGGYIGGRLSEKGNDVTFLVRQKRAEQLKKTGLVIHSEKGNVSFQPELIS
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCEEECCCCCEEECCHHCC
AGETGQFDVVIIASKAYSLGQVIEDVKPFIHQESVIIPFLNGYRHYEQLFAAFSKEQVLG
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
GLCFIESALDNKGEIHHTSASHRFVFGEWNGERTERIRALEEAFSGVKAEVIISGHIEKD
HHHHHHHHHCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHH
IWKKYLFIAAQAGITTLFQRPLGPILATEAGRHTAQTLIGEICTVLRKEGVPADPALEEE
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SFRTMTSMSYHMKSSMLRDMENGQTTEGDHLHGFLIEKAKRLSLAAPVLETVYANLQMYE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
AEK
CCC
>Mature Secondary Structure
MKFLVVGAGGVGGYIGGRLSEKGNDVTFLVRQKRAEQLKKTGLVIHSEKGNVSFQPELIS
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCEEECCCCCEEECCHHCC
AGETGQFDVVIIASKAYSLGQVIEDVKPFIHQESVIIPFLNGYRHYEQLFAAFSKEQVLG
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
GLCFIESALDNKGEIHHTSASHRFVFGEWNGERTERIRALEEAFSGVKAEVIISGHIEKD
HHHHHHHHHCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHH
IWKKYLFIAAQAGITTLFQRPLGPILATEAGRHTAQTLIGEICTVLRKEGVPADPALEEE
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SFRTMTSMSYHMKSSMLRDMENGQTTEGDHLHGFLIEKAKRLSLAAPVLETVYANLQMYE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
AEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.0 {NADPH}} [C]

Substrates: 2-Dehydropantoate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 2-Dehydropantoate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> Nicotinamide adenine dinucleotide phosphate + (R)-Pantoate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377