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The map label for this gene is ykwC

Identifier: 16078460

GI number: 16078460

Start: 1465733

End: 1466599

Strand: Direct

Name: ykwC

Synonym: BSU13960

Alternate gene names: 16078460

Gene position: 1465733-1466599 (Clockwise)

Preceding gene: 255767323

Following gene: 16078462

Centisome position: 34.77

GC content: 43.83

Gene sequence:

>867_bases
TTGAAAAAAACAATTGGTTTTATCGGTCTTGGAGTAATGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCC
TGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAG
ATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAAT
GGAATTATTGAAAACGCAAAAGAAGGTGCATATCTTATTGATATGACGACATCTAAGCCTTCGCTTGCGAAGAAGATTGC
TGAAGCGGCCAAAGAAAAAGCATTGTTCGCTTTAGACGCTCCTGTTTCCGGTGGAGACATCGGTGCACAAAACGGAACAC
TCGCGATTATGGTCGGTGGAGAAAAAGAAGCTTTTGAAGCCTGCATGCCGATTTTCTCTTTAATGGGAGAGAATATTCAG
TATCAAGGACCGGCTGGAAGCGGACAGCATACCAAGATGTGCAATCAGATTGCAATTGCAGCCGGTATGATCGGTGTTGC
TGAAGCGATGGCTTATGCCCAAAAATCAGGCCTAGAGCCGGAAAATGTTCTGAAAAGCATCACAACGGGCGCAGCCGGAA
GCTGGTCATTGTCAAATCTTGCTCCCCGTATGCTGCAAGGCAACTTTGAACCGGGATTTTATGTGAAGCATTTTATTAAG
GATATGGGAATTGCACTAGAGGAAGCAGAATTAATGGGGGAAGAAATGCCAGGCTTGTCGCTCGCAAAATCTTTATATGA
CAAGCTAGCTGCTCAAGGTGAAGAAAACAGCGGAACACAGAGTATATATAAGCTTTGGGTGAAATAA

Upstream 100 bases:

>100_bases
TTTTTATTTTATAAGATGAATAGATATGGTAGTCTAGAAAATGAAGGAAAAATGATAAATTTTAACTAAAATCTAAACGA
AACATCTGGGAGGAATGACC

Downstream 100 bases:

>100_bases
AAAAAGATTGCCTGTACGAAACAGGCAATCTTTTTTTATTATTTTCCGATGAATTGTTGTGTCCAGATGCTTCCAGACTC
AACGTAACCAACACCGATGT

Product: beta-hydroxyacid dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIITMVGYPSDVEEVYFGSN
GIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQ
YQGPAGSGQHTKMCNQIAIAAGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIK
DMGIALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSIYKLWVK

Sequences:

>Translated_288_residues
MKKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIITMVGYPSDVEEVYFGSN
GIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQ
YQGPAGSGQHTKMCNQIAIAAGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIK
DMGIALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSIYKLWVK
>Mature_288_residues
MKKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIITMVGYPSDVEEVYFGSN
GIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQ
YQGPAGSGQHTKMCNQIAIAAGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIK
DMGIALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSIYKLWVK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=32.9896907216495, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=27.0175438596491, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI145693186, Length=269, Percent_Identity=38.6617100371747, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786719, Length=269, Percent_Identity=37.9182156133829, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1790315, Length=272, Percent_Identity=31.9852941176471, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=27.6223776223776, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=30.4029304029304, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=32.7702702702703, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=32.7702702702703, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=26.7605633802817, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YKWC_BACSU (O34948)

Other databases:

- EMBL:   AJ222587
- EMBL:   AL009126
- PIR:   B69870
- RefSeq:   NP_389279.1
- ProteinModelPortal:   O34948
- SMR:   O34948
- EnsemblBacteria:   EBBACT00000002607
- GeneID:   939723
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13960
- NMPDR:   fig|224308.1.peg.1398
- GenoList:   BSU13960
- GeneTree:   EBGT00050000001992
- HOGENOM:   HBG729179
- OMA:   AEAMAYA
- PhylomeDB:   O34948
- ProtClustDB:   CLSK2393382
- BioCyc:   BSUB:BSU13960-MONOMER
- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981
- PRINTS:   PR00076

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: 1.1.1.31

Molecular weight: Translated: 30712; Mature: 30712

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 172-172 BINDING 97-97 BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEAD
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCC
VIITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDA
EEEEEECCCCCHHHHEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEECC
PVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHH
AGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
DMGIALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSIYKLWVK
HHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEAD
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCC
VIITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDA
EEEEEECCCCCHHHHEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEECC
PVSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHH
AGMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
DMGIALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSIYKLWVK
HHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377