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The map label for this gene is splB

Identifier: 16078457

GI number: 16078457

Start: 1461770

End: 1462798

Strand: Direct

Name: splB

Synonym: BSU13930

Alternate gene names: 16078457

Gene position: 1461770-1462798 (Clockwise)

Preceding gene: 16078456

Following gene: 255767323

Centisome position: 34.68

GC content: 41.89

Gene sequence:

>1029_bases
ATGCAGAACCCATTTGTTCCGCAGCTTGTGTATATAGAACCGAGGGCGCTGGAATATCCGCTGGGCCAAGAATTACAAGA
TAAATTTGAGAATATGGGTATTGAAATCAGAGAAACGACATCCCACAATCAGGTGCGTAATATCCCTGGGAAAAATCATC
TTCAGCAATATCGCAATGCGAAATCAACTTTAGTTATCGGTGTCCGGAAAACATTAAAGTTTGATTCATCCAAACCCTCG
GCCGAATATGCCATTCCGTTTGCAACAGGCTGCATGGGGCATTGTCATTACTGCTACCTGCAAACAACCATGGGATCAAA
GCCGTATATCAGAACTTATGTAAACGTCGAAGAGATTTTAGATCAGGCAGATAAGTATATGAAGGAGCGCGCACCAGAGT
TCACAAGGTTCGAAGCATCATGTACGTCAGACATTGTAGGAATTGATCATCTGACACACACGCTGAAGCGCGCCATTGAA
CATTTTGGCCAAAGTGATCTCGGAAAGCTCCGATTTGTAACGAAATTTCATCATGTCGATCACCTATTAGACGCAAAGCA
TAACGGGAAAACGAGATTCAGATTCAGTATTAATGCCGACTATGTGATTAAAAACTTTGAGCCGGGAACTTCACCTCTTG
ATAAGCGGATAGAAGCGGCAGTAAAAGTTGCAAAAGCAGGCTACCCGCTAGGCTTTATTGTTGCTCCGATTTATATTCAT
GAAGGCTGGGAAGAAGGATACAGACATCTGTTTGAAAAGCTAGATGCTGCTTTGCCGCAGGACGTTAGACATGACATTAC
GTTTGAATTAATTCAACACCGTTTTACAAAACCGGCCAAACGAGTGATAGAGAAAAATTATCCGAAGACGAAGCTCGAAT
TAGATGAAGAAAAGCGCCGTTATAAATGGGGCCGTTACGGGATCGGAAAATATATTTATCAGAAAGATGAAGAGCATGCA
CTTCGAGAGGCACTTGAATCCTATATTGATACCTTTTTCCCTAACGCAAAAATTGAATATTTCACTTAA

Upstream 100 bases:

>100_bases
TTGATATTGATCCGTATGAGTAACAAGGAAATCATTACAACTCATATCCTTTCCGCCTAGTGAGAAAAGTAACGTTAGTA
AAGGAAAAGGATGTGGCATC

Downstream 100 bases:

>100_bases
ACGGGCTGTTGTGATCAACAGCTCGCTTTTTTATAAAAAACATTTTTCCATTCCATCAAAACGCCGTAATTCAATGATTC
TTTATCCTCCAAATAATTGC

Product: spore photoproduct (thymine dimer) lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MQNPFVPQLVYIEPRALEYPLGQELQDKFENMGIEIRETTSHNQVRNIPGKNHLQQYRNAKSTLVIGVRKTLKFDSSKPS
AEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNVEEILDQADKYMKERAPEFTRFEASCTSDIVGIDHLTHTLKRAIE
HFGQSDLGKLRFVTKFHHVDHLLDAKHNGKTRFRFSINADYVIKNFEPGTSPLDKRIEAAVKVAKAGYPLGFIVAPIYIH
EGWEEGYRHLFEKLDAALPQDVRHDITFELIQHRFTKPAKRVIEKNYPKTKLELDEEKRRYKWGRYGIGKYIYQKDEEHA
LREALESYIDTFFPNAKIEYFT

Sequences:

>Translated_342_residues
MQNPFVPQLVYIEPRALEYPLGQELQDKFENMGIEIRETTSHNQVRNIPGKNHLQQYRNAKSTLVIGVRKTLKFDSSKPS
AEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNVEEILDQADKYMKERAPEFTRFEASCTSDIVGIDHLTHTLKRAIE
HFGQSDLGKLRFVTKFHHVDHLLDAKHNGKTRFRFSINADYVIKNFEPGTSPLDKRIEAAVKVAKAGYPLGFIVAPIYIH
EGWEEGYRHLFEKLDAALPQDVRHDITFELIQHRFTKPAKRVIEKNYPKTKLELDEEKRRYKWGRYGIGKYIYQKDEEHA
LREALESYIDTFFPNAKIEYFT
>Mature_342_residues
MQNPFVPQLVYIEPRALEYPLGQELQDKFENMGIEIRETTSHNQVRNIPGKNHLQQYRNAKSTLVIGVRKTLKFDSSKPS
AEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNVEEILDQADKYMKERAPEFTRFEASCTSDIVGIDHLTHTLKRAIE
HFGQSDLGKLRFVTKFHHVDHLLDAKHNGKTRFRFSINADYVIKNFEPGTSPLDKRIEAAVKVAKAGYPLGFIVAPIYIH
EGWEEGYRHLFEKLDAALPQDVRHDITFELIQHRFTKPAKRVIEKNYPKTKLELDEEKRRYKWGRYGIGKYIYQKDEEHA
LREALESYIDTFFPNAKIEYFT

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPL_BACSU (P37956)

Other databases:

- EMBL:   L08809
- EMBL:   AL009126
- EMBL:   X97385
- PIR:   C47084
- RefSeq:   NP_389276.1
- ProteinModelPortal:   P37956
- EnsemblBacteria:   EBBACT00000003923
- GeneID:   939248
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13930
- NMPDR:   fig|224308.1.peg.1395
- GenoList:   BSU13930
- GeneTree:   EBGT00050000002267
- HOGENOM:   HBG305559
- OMA:   FEASCTS
- ProtClustDB:   CLSK873435
- BioCyc:   BSUB:BSU13930-MONOMER
- BioCyc:   MetaCyc:MONOMER-15020
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR00620

Pfam domain/function: NA

EC number: =4.1.99.14

Molecular weight: Translated: 39943; Mature: 39943

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPFVPQLVYIEPRALEYPLGQELQDKFENMGIEIRETTSHNQVRNIPGKNHLQQYRNA
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCCHHHCCCCHHHHHHHHCC
KSTLVIGVRKTLKFDSSKPSAEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNVEEIL
CCEEEEEEHHHHCCCCCCCCCCEECCHHHCCCCCCEEEEEEECCCCCCCEEEECCHHHHH
DQADKYMKERAPEFTRFEASCTSDIVGIDHLTHTLKRAIEHFGQSDLGKLRFVTKFHHVD
HHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
HLLDAKHNGKTRFRFSINADYVIKNFEPGTSPLDKRIEAAVKVAKAGYPLGFIVAPIYIH
HHHCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
EGWEEGYRHLFEKLDAALPQDVRHDITFELIQHRFTKPAKRVIEKNYPKTKLELDEEKRR
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHH
YKWGRYGIGKYIYQKDEEHALREALESYIDTFFPNAKIEYFT
HCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MQNPFVPQLVYIEPRALEYPLGQELQDKFENMGIEIRETTSHNQVRNIPGKNHLQQYRNA
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCCHHHCCCCHHHHHHHHCC
KSTLVIGVRKTLKFDSSKPSAEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNVEEIL
CCEEEEEEHHHHCCCCCCCCCCEECCHHHCCCCCCEEEEEEECCCCCCCEEEECCHHHHH
DQADKYMKERAPEFTRFEASCTSDIVGIDHLTHTLKRAIEHFGQSDLGKLRFVTKFHHVD
HHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
HLLDAKHNGKTRFRFSINADYVIKNFEPGTSPLDKRIEAAVKVAKAGYPLGFIVAPIYIH
HHHCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
EGWEEGYRHLFEKLDAALPQDVRHDITFELIQHRFTKPAKRVIEKNYPKTKLELDEEKRR
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHH
YKWGRYGIGKYIYQKDEEHALREALESYIDTFFPNAKIEYFT
HCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924