Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is trpS

Identifier: 16078207

GI number: 16078207

Start: 1218113

End: 1219105

Strand: Reverse

Name: trpS

Synonym: BSU11420

Alternate gene names: 16078207

Gene position: 1219105-1218113 (Counterclockwise)

Preceding gene: 16078216

Following gene: 16078197

Centisome position: 28.92

GC content: 45.32

Gene sequence:

>993_bases
ATGAAACAAACGATTTTTTCAGGCATTCAGCCAAGCGGCTCAGTGACGCTCGGCAACTATATCGGTGCAATGAAGCAGTT
TGTCGAACTGCAGCATGATTATAACAGCTATTTTTGCATCGTCGATCAGCATGCGATAACTGTTCCTCAAGACCGGCTTG
AGCTTAGAAAGAATATCCGCAATCTCGCGGCGCTTTACTTAGCTGTCGGACTTGATCCAGAAAAAGCAACATTGTTTATT
CAGTCAGAGGTCCCCGCACATGCGCAGGCCGGATGGATGATGCAGTGTGTCGCCTATATCGGCGAGCTTGAGCGGATGAC
TCAATTTAAGGACAAATCCAAAGGCAATGAAGCTGTCGTCTCCGGCCTGTTAACATATCCGCCGCTGATGGCCGCTGATA
TTCTGCTGTACGGAACGGATCTTGTACCTGTCGGCGAGGATCAAAAGCAGCACCTTGAGCTGACGCGGAATCTTGCAGAA
CGCTTCAACAAAAAATACAACGACATCTTTACGATTCCGGAAGTGAAAATTCCAAAAGTCGGTGCACGTATCATGTCTCT
GAATGATCCGCTGAAGAAAATGAGCAAATCTGATCCGAATCAGAAAGCTTATATTACATTGCTGGATGAGCCGAAGCAGC
TTGAAAAGAAAATCAAAAGCGCAGTAACGGATTCTGAAGGCATTGTCAAATTTGATAAGGAAAACAAACCGGGCGTTTCC
AACCTTCTTACAATTTATTCAATCCTCGGCAATACGACAATTGAAGAGCTTGAAGCAAAGTACGAAGGAAAAGGCTACGG
CGAGTTTAAAGGTGATTTGGCAGAAGTCGTAGTGAACGCATTAAAACCGATCCAGGACCGCTATTACGAGCTGATAGAAT
CTGAAGAATTAGACCGGATTCTTGATGAAGGCGCGGAACGAGCGAATCGGACAGCAAACAAAATGCTGAAAAAAATGGAG
AATGCCATGGGTCTTGGAAGAAAAAGACGCTAA

Upstream 100 bases:

>100_bases
ATGGAATAATCAGGGTGGTACCACGGTTCATTCGTCCCTTTTTTACAGGGGAAGAATGAGCCTTTTTTATTATGTTTTAA
GAAATGAGGTTGATGTTTTC

Downstream 100 bases:

>100_bases
TCAAAAAACCGCTCTTTGCAAAGAGCGGTTTTTTTCAGTTGACCTTTGATTCGTTTTCCATTTCCCAAAGCTTTTCGAAA
AAAGGCTGTCCTTTTATTAG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKQTIFSGIQPSGSVTLGNYIGAMKQFVELQHDYNSYFCIVDQHAITVPQDRLELRKNIRNLAALYLAVGLDPEKATLFI
QSEVPAHAQAGWMMQCVAYIGELERMTQFKDKSKGNEAVVSGLLTYPPLMAADILLYGTDLVPVGEDQKQHLELTRNLAE
RFNKKYNDIFTIPEVKIPKVGARIMSLNDPLKKMSKSDPNQKAYITLLDEPKQLEKKIKSAVTDSEGIVKFDKENKPGVS
NLLTIYSILGNTTIEELEAKYEGKGYGEFKGDLAEVVVNALKPIQDRYYELIESEELDRILDEGAERANRTANKMLKKME
NAMGLGRKRR

Sequences:

>Translated_330_residues
MKQTIFSGIQPSGSVTLGNYIGAMKQFVELQHDYNSYFCIVDQHAITVPQDRLELRKNIRNLAALYLAVGLDPEKATLFI
QSEVPAHAQAGWMMQCVAYIGELERMTQFKDKSKGNEAVVSGLLTYPPLMAADILLYGTDLVPVGEDQKQHLELTRNLAE
RFNKKYNDIFTIPEVKIPKVGARIMSLNDPLKKMSKSDPNQKAYITLLDEPKQLEKKIKSAVTDSEGIVKFDKENKPGVS
NLLTIYSILGNTTIEELEAKYEGKGYGEFKGDLAEVVVNALKPIQDRYYELIESEELDRILDEGAERANRTANKMLKKME
NAMGLGRKRR
>Mature_330_residues
MKQTIFSGIQPSGSVTLGNYIGAMKQFVELQHDYNSYFCIVDQHAITVPQDRLELRKNIRNLAALYLAVGLDPEKATLFI
QSEVPAHAQAGWMMQCVAYIGELERMTQFKDKSKGNEAVVSGLLTYPPLMAADILLYGTDLVPVGEDQKQHLELTRNLAE
RFNKKYNDIFTIPEVKIPKVGARIMSLNDPLKKMSKSDPNQKAYITLLDEPKQLEKKIKSAVTDSEGIVKFDKENKPGVS
NLLTIYSILGNTTIEELEAKYEGKGYGEFKGDLAEVVVNALKPIQDRYYELIESEELDRILDEGAERANRTANKMLKKME
NAMGLGRKRR

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=327, Percent_Identity=39.4495412844037, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI41352700, Length=175, Percent_Identity=50.2857142857143, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=54.8780487804878, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71982800, Length=330, Percent_Identity=39.3939393939394, Blast_Score=218, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI71982793, Length=330, Percent_Identity=39.3939393939394, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6320474, Length=344, Percent_Identity=40.6976744186046, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24666151, Length=325, Percent_Identity=40, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_BACSU (P21656)

Other databases:

- EMBL:   M24068
- EMBL:   AL009126
- PIR:   JT0481
- RefSeq:   NP_389024.1
- ProteinModelPortal:   P21656
- SMR:   P21656
- EnsemblBacteria:   EBBACT00000001677
- GeneID:   939361
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11420
- NMPDR:   fig|224308.1.peg.1143
- GenoList:   BSU11420
- GeneTree:   EBGT00050000002240
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- PhylomeDB:   P21656
- ProtClustDB:   PRK00927
- BioCyc:   BSUB:BSU11420-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37197; Mature: 37197

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKQTIFSGIQPSGSVTLGNYIGAMKQFVELQHDYNSYFCIVDQHAITVPQDRLELRKNIR
CCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCEECCCHHHHHHHHHHH
NLAALYLAVGLDPEKATLFIQSEVPAHAQAGWMMQCVAYIGELERMTQFKDKSKGNEAVV
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SGLLTYPPLMAADILLYGTDLVPVGEDQKQHLELTRNLAERFNKKYNDIFTIPEVKIPKV
HHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHH
GARIMSLNDPLKKMSKSDPNQKAYITLLDEPKQLEKKIKSAVTDSEGIVKFDKENKPGVS
HHHHHHCCHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
NLLTIYSILGNTTIEELEAKYEGKGYGEFKGDLAEVVVNALKPIQDRYYELIESEELDRI
HHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEGAERANRTANKMLKKMENAMGLGRKRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQTIFSGIQPSGSVTLGNYIGAMKQFVELQHDYNSYFCIVDQHAITVPQDRLELRKNIR
CCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCEECCCHHHHHHHHHHH
NLAALYLAVGLDPEKATLFIQSEVPAHAQAGWMMQCVAYIGELERMTQFKDKSKGNEAVV
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SGLLTYPPLMAADILLYGTDLVPVGEDQKQHLELTRNLAERFNKKYNDIFTIPEVKIPKV
HHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHH
GARIMSLNDPLKKMSKSDPNQKAYITLLDEPKQLEKKIKSAVTDSEGIVKFDKENKPGVS
HHHHHHCCHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
NLLTIYSILGNTTIEELEAKYEGKGYGEFKGDLAEVVVNALKPIQDRYYELIESEELDRI
HHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEGAERANRTANKMLKKMENAMGLGRKRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3149612; 9384377; 2494170