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The map label for this gene is hemY

Identifier: 16078078

GI number: 16078078

Start: 1088197

End: 1089609

Strand: Direct

Name: hemY

Synonym: BSU10140

Alternate gene names: 16078078

Gene position: 1088197-1089609 (Clockwise)

Preceding gene: 16078077

Following gene: 16078079

Centisome position: 25.81

GC content: 48.48

Gene sequence:

>1413_bases
ATGAGTGACGGCAAAAAACATGTAGTCATCATCGGCGGCGGCATTACCGGTTTAGCCGCCGCCTTCTATATGGAAAAAGA
AATCAAAGAAAAGAATCTGCCGCTTGAGCTGACACTTGTTGAGGCAAGTCCGAGAGTCGGCGGGAAAATCCAGACTGTCA
AGAAAGACGGCTATATCATCGAAAGAGGGCCAGACTCATTTCTGGAACGAAAGAAAAGCGCCCCGCAGCTTGTTAAAGAC
TTAGGTCTTGAGCATTTGCTTGTCAACAATGCGACCGGGCAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCC
GAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGA
GAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTC
GGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCAGGGATCTACGCAGGCGACATTGACAAGCTCAGCCTGAT
GTCGACATTTCCGCAATTTTATCAGACGGAACAAAAGCATAGAAGCCTGATTCTCGGCATGAAAAAAACAAGGCCTCAAG
GCTCAGGCCAGCAGCTGACGGCAAAAAAACAAGGGCAGTTCCAAACTCTGTCAACCGGTTTGCAGACCCTTGTAGAAGAG
ATCGAAAAGCAGTTAAAGCTGACGAAGGTGTATAAAGGCACAAAAGTGACCAAACTTAGCCATAGCGGCTCTTGCTATTC
GCTCGAACTGGATAACGGCGTCACACTTGATGCTGATTCAGTAATTGTGACTGCTCCGCATAAAGCGGCTGCGGGAATGC
TTTCTGAGCTTCCTGCCATTTCTCATTTGAAAAATATGCACTCCACATCCGTGGCAAACGTCGCTTTAGGTTTCCCTGAA
GGCTCCGTCCAAATGGAGCATGAGGGCACGGGTTTTGTCATTTCAAGAAACAGTGACTTTGCGATCACAGCCTGTACGTG
GACGAATAAAAAATGGCCGCACGCAGCGCCGGAAGGCAAAACGCTGCTTCGGGCATATGTCGGAAAAGCCGGAGACGAAT
CCATTGTCGATCTATCAGATAATGACATTATCAACATTGTGTTAGAAGACTTAAAGAAAGTCATGAACATAAACGGCGAG
CCGGAAATGACATGTGTAACCCGATGGCATGAAAGCATGCCGCAGTACCATGTCGGCCATAAGCAGCGTATCAAGGAGCT
GCGTGAAGCACTTGCATCTGCGTATCCGGGTGTTTATATGACAGGCGCTTCTTTCGAAGGTGTCGGCATTCCCGACTGCA
TTGATCAAGGAAAAGCTGCCGTGTCTGACGCGCTTACCTATTTATTCAGCTAA

Upstream 100 bases:

>100_bases
GCTATTACCGGCCGGAAATGCCAAATGCCAAGCCTGAATTTATTGATGCTTTGGCAACAGTCGTATTAAAAAAATTAGGA
CGTTAAAGAAGGCGATGAAC

Downstream 100 bases:

>100_bases
AACCTCCGCTTTATCGCGGAGGTTTTTTTGATGTGCAATGGCAATATTGTGTAAAAAATGATTGAATTGAACAGCAGGTT
ATGATACATTTGCATATGTA

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: PPO

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSDGKKHVVIIGGGITGLAAAFYMEKEIKEKNLPLELTLVEASPRVGGKIQTVKKDGYIIERGPDSFLERKKSAPQLVKD
LGLEHLLVNNATGQSYVLVNRTLHPMPKGAVMGIPTKIAPFVSTGLFSLSGKARAAMDFILPASKTKDDQSLGEFFRRRV
GDEVVENLIEPLLSGIYAGDIDKLSLMSTFPQFYQTEQKHRSLILGMKKTRPQGSGQQLTAKKQGQFQTLSTGLQTLVEE
IEKQLKLTKVYKGTKVTKLSHSGSCYSLELDNGVTLDADSVIVTAPHKAAAGMLSELPAISHLKNMHSTSVANVALGFPE
GSVQMEHEGTGFVISRNSDFAITACTWTNKKWPHAAPEGKTLLRAYVGKAGDESIVDLSDNDIINIVLEDLKKVMNINGE
PEMTCVTRWHESMPQYHVGHKQRIKELREALASAYPGVYMTGASFEGVGIPDCIDQGKAAVSDALTYLFS

Sequences:

>Translated_470_residues
MSDGKKHVVIIGGGITGLAAAFYMEKEIKEKNLPLELTLVEASPRVGGKIQTVKKDGYIIERGPDSFLERKKSAPQLVKD
LGLEHLLVNNATGQSYVLVNRTLHPMPKGAVMGIPTKIAPFVSTGLFSLSGKARAAMDFILPASKTKDDQSLGEFFRRRV
GDEVVENLIEPLLSGIYAGDIDKLSLMSTFPQFYQTEQKHRSLILGMKKTRPQGSGQQLTAKKQGQFQTLSTGLQTLVEE
IEKQLKLTKVYKGTKVTKLSHSGSCYSLELDNGVTLDADSVIVTAPHKAAAGMLSELPAISHLKNMHSTSVANVALGFPE
GSVQMEHEGTGFVISRNSDFAITACTWTNKKWPHAAPEGKTLLRAYVGKAGDESIVDLSDNDIINIVLEDLKKVMNINGE
PEMTCVTRWHESMPQYHVGHKQRIKELREALASAYPGVYMTGASFEGVGIPDCIDQGKAAVSDALTYLFS
>Mature_469_residues
SDGKKHVVIIGGGITGLAAAFYMEKEIKEKNLPLELTLVEASPRVGGKIQTVKKDGYIIERGPDSFLERKKSAPQLVKDL
GLEHLLVNNATGQSYVLVNRTLHPMPKGAVMGIPTKIAPFVSTGLFSLSGKARAAMDFILPASKTKDDQSLGEFFRRRVG
DEVVENLIEPLLSGIYAGDIDKLSLMSTFPQFYQTEQKHRSLILGMKKTRPQGSGQQLTAKKQGQFQTLSTGLQTLVEEI
EKQLKLTKVYKGTKVTKLSHSGSCYSLELDNGVTLDADSVIVTAPHKAAAGMLSELPAISHLKNMHSTSVANVALGFPEG
SVQMEHEGTGFVISRNSDFAITACTWTNKKWPHAAPEGKTLLRAYVGKAGDESIVDLSDNDIINIVLEDLKKVMNINGEP
EMTCVTRWHESMPQYHVGHKQRIKELREALASAYPGVYMTGASFEGVGIPDCIDQGKAAVSDALTYLFS

Specific function: Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Also oxidizes the pathway intermediate coproporphyrinogen-III

COG id: COG1232

COG function: function code H; Protoporphyrinogen oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protoporphyrinogen oxidase family

Homologues:

Organism=Homo sapiens, GI170650615, Length=492, Percent_Identity=28.2520325203252, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI4506001, Length=492, Percent_Identity=28.2520325203252, Blast_Score=139, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24649745, Length=467, Percent_Identity=25.6959314775161, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPOX_BACSU (P32397)

Other databases:

- EMBL:   M97208
- EMBL:   Y14083
- EMBL:   AL009126
- PIR:   D47045
- RefSeq:   NP_388895.1
- PDB:   3I6D
- PDBsum:   3I6D
- ProteinModelPortal:   P32397
- SMR:   P32397
- EnsemblBacteria:   EBBACT00000003009
- GeneID:   936311
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10140
- NMPDR:   fig|224308.1.peg.1014
- GenoList:   BSU10140
- GeneTree:   EBGT00050000001429
- HOGENOM:   HBG450176
- OMA:   EGSERLG
- ProtClustDB:   PRK11883
- BioCyc:   BSUB:BSU10140-MONOMER
- BRENDA:   1.3.3.4
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR002937
- InterPro:   IPR016040
- InterPro:   IPR004572
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00562

Pfam domain/function: PF01593 Amino_oxidase

EC number: =1.3.3.4

Molecular weight: Translated: 51204; Mature: 51072

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGKKHVVIIGGGITGLAAAFYMEKEIKEKNLPLELTLVEASPRVGGKIQTVKKDGYII
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCEEE
ERGPDSFLERKKSAPQLVKDLGLEHLLVNNATGQSYVLVNRTLHPMPKGAVMGIPTKIAP
ECCCHHHHHHHCCCHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCEECCCCHHHH
FVSTGLFSLSGKARAAMDFILPASKTKDDQSLGEFFRRRVGDEVVENLIEPLLSGIYAGD
HHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDKLSLMSTFPQFYQTEQKHRSLILGMKKTRPQGSGQQLTAKKQGQFQTLSTGLQTLVEE
CHHHHHHHHHHHHHHHHHHHHHHEECHHHCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHH
IEKQLKLTKVYKGTKVTKLSHSGSCYSLELDNGVTLDADSVIVTAPHKAAAGMLSELPAI
HHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCEEECCCCEEEECCCHHHHHHHHHCHHH
SHLKNMHSTSVANVALGFPEGSVQMEHEGTGFVISRNSDFAITACTWTNKKWPHAAPEGK
HHHHHHHCCCHHEEEEECCCCCEEEEECCCEEEEECCCCEEEEEEEECCCCCCCCCCCHH
TLLRAYVGKAGDESIVDLSDNDIINIVLEDLKKVMNINGEPEMTCVTRWHESMPQYHVGH
HHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCH
KQRIKELREALASAYPGVYMTGASFEGVGIPDCIDQGKAAVSDALTYLFS
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SDGKKHVVIIGGGITGLAAAFYMEKEIKEKNLPLELTLVEASPRVGGKIQTVKKDGYII
CCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCEEE
ERGPDSFLERKKSAPQLVKDLGLEHLLVNNATGQSYVLVNRTLHPMPKGAVMGIPTKIAP
ECCCHHHHHHHCCCHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCEECCCCHHHH
FVSTGLFSLSGKARAAMDFILPASKTKDDQSLGEFFRRRVGDEVVENLIEPLLSGIYAGD
HHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDKLSLMSTFPQFYQTEQKHRSLILGMKKTRPQGSGQQLTAKKQGQFQTLSTGLQTLVEE
CHHHHHHHHHHHHHHHHHHHHHHEECHHHCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHH
IEKQLKLTKVYKGTKVTKLSHSGSCYSLELDNGVTLDADSVIVTAPHKAAAGMLSELPAI
HHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCEEECCCCEEEECCCHHHHHHHHHCHHH
SHLKNMHSTSVANVALGFPEGSVQMEHEGTGFVISRNSDFAITACTWTNKKWPHAAPEGK
HHHHHHHCCCHHEEEEECCCCCEEEEECCCEEEEECCCCEEEEEEEECCCCCCCCCCCHH
TLLRAYVGKAGDESIVDLSDNDIINIVLEDLKKVMNINGEPEMTCVTRWHESMPQYHVGH
HHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCH
KQRIKELREALASAYPGVYMTGASFEGVGIPDCIDQGKAAVSDALTYLFS
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1459957; 9579061; 9384377; 8288631