Click here to switch to the map view.

The map label for this gene is prsA

Identifier: 16078059

GI number: 16078059

Start: 1070364

End: 1071242

Strand: Reverse

Name: prsA

Synonym: BSU09950

Alternate gene names: 16078059

Gene position: 1071242-1070364 (Counterclockwise)

Preceding gene: 255767232

Following gene: 255767228

Centisome position: 25.41

GC content: 39.93

Gene sequence:

>879_bases
ATGAAGAAAATCGCAATAGCAGCTATCACTGCTACAAGCATCCTCGCTCTCAGTGCTTGCAGCAGCGGCGACAAAGAAGT
TATCGCAAAAACAGACGCAGGCGATGTCACAAAAGGCGAGCTTTACACAAACATGAAGAAAACAGCTGGCGCAAGCGTAC
TGACACAGCTAGTGCAAGAAAAAGTATTGGACAAGAAGTATAAAGTTTCGGATAAAGAAATTGACAACAAGCTGAAAGAA
TACAAAACGCAGCTTGGCGATCAATATACTGCCCTCGAAAAGCAATATGGCAAAGATTACCTGAAAGAACAAGTAAAATA
TGAATTGCTGACACAAAAAGCGGCTAAAGATAACATCAAAGTAACAGACGCCGATATCAAAGAGTACTGGGAAGGCTTAA
AAGGCAAAATCCGTGCAAGCCACATCCTTGTTGCTGATAAAAAGACAGCTGAAGAAGTAGAGAAAAAGCTGAAAAAAGGC
GAGAAGTTTGAAGACCTTGCGAAAGAATACTCAACAGACAGCTCTGCTTCAAAAGGCGGGGATCTTGGCTGGTTCGCAAA
AGAAGGCCAAATGGACGAAACATTCAGCAAAGCTGCATTCAAATTAAAAACAGGTGAAGTCAGTGATCCTGTCAAAACGC
AATACGGCTACCATATCATTAAAAAGACAGAAGAACGCGGCAAATATGATGATATGAAAAAAGAACTGAAATCTGAAGTG
CTTGAACAAAAATTAAATGACAACGCAGCTGTTCAGGAAGCTGTTCAAAAAGTCATGAAGAAGGCTGACATCGAAGTAAA
AGATAAAGATCTGAAAGACACATTTAATACATCTTCAACAAGCAACAGCACTTCTTCATCTTCAAGCAATTCTAAATAA

Upstream 100 bases:

>100_bases
GACGAATGCCCTTTATTTAAAATCTTTTTACAGAAAGCCACAATATGTTATGATACGCATAGGTAATTTAATTTGGAATG
ATTAGGAGTGTTTGAAAACA

Downstream 100 bases:

>100_bases
TAAAAAAAGCTGTGCGGCTCATTGAGCCGCACAGCTTTTTTTATGCGATGGAATGGTTTTGATTTTCTTCTTCATGCTGC
TGAGAACTGCGGCGTTCTCG

Product: molecular chaperone lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKKIAIAAITATSILALSACSSGDKEVIAKTDAGDVTKGELYTNMKKTAGASVLTQLVQEKVLDKKYKVSDKEIDNKLKE
YKTQLGDQYTALEKQYGKDYLKEQVKYELLTQKAAKDNIKVTDADIKEYWEGLKGKIRASHILVADKKTAEEVEKKLKKG
EKFEDLAKEYSTDSSASKGGDLGWFAKEGQMDETFSKAAFKLKTGEVSDPVKTQYGYHIIKKTEERGKYDDMKKELKSEV
LEQKLNDNAAVQEAVQKVMKKADIEVKDKDLKDTFNTSSTSNSTSSSSSNSK

Sequences:

>Translated_292_residues
MKKIAIAAITATSILALSACSSGDKEVIAKTDAGDVTKGELYTNMKKTAGASVLTQLVQEKVLDKKYKVSDKEIDNKLKE
YKTQLGDQYTALEKQYGKDYLKEQVKYELLTQKAAKDNIKVTDADIKEYWEGLKGKIRASHILVADKKTAEEVEKKLKKG
EKFEDLAKEYSTDSSASKGGDLGWFAKEGQMDETFSKAAFKLKTGEVSDPVKTQYGYHIIKKTEERGKYDDMKKELKSEV
LEQKLNDNAAVQEAVQKVMKKADIEVKDKDLKDTFNTSSTSNSTSSSSSNSK
>Mature_292_residues
MKKIAIAAITATSILALSACSSGDKEVIAKTDAGDVTKGELYTNMKKTAGASVLTQLVQEKVLDKKYKVSDKEIDNKLKE
YKTQLGDQYTALEKQYGKDYLKEQVKYELLTQKAAKDNIKVTDADIKEYWEGLKGKIRASHILVADKKTAEEVEKKLKKG
EKFEDLAKEYSTDSSASKGGDLGWFAKEGQMDETFSKAAFKLKTGEVSDPVKTQYGYHIIKKTEERGKYDDMKKELKSEV
LEQKLNDNAAVQEAVQKVMKKADIEVKDKDLKDTFNTSSTSNSTSSSSSNSK

Specific function: Essential protein that plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Has PPIase activity but it is not essential for its function in vivo

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain

Homologues:

Organism=Homo sapiens, GI5453898, Length=116, Percent_Identity=39.6551724137931, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790211, Length=83, Percent_Identity=44.578313253012, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537235, Length=90, Percent_Identity=41.1111111111111, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322478, Length=65, Percent_Identity=53.8461538461538, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRSA_BACSU (P24327)

Other databases:

- EMBL:   X57271
- EMBL:   Y14077
- EMBL:   AL009126
- EMBL:   M22909
- PIR:   S15269
- RefSeq:   NP_388876.1
- PDB:   1ZK6
- PDBsum:   1ZK6
- ProteinModelPortal:   P24327
- SMR:   P24327
- EnsemblBacteria:   EBBACT00000003644
- GeneID:   939294
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09950
- NMPDR:   fig|224308.1.peg.995
- GenoList:   BSU09950
- GeneTree:   EBGT00070000032266
- HOGENOM:   HBG712792
- OMA:   MLVEYAV
- PhylomeDB:   P24327
- ProtClustDB:   CLSK886987
- BioCyc:   BSUB:BSU09950-MONOMER
- BRENDA:   5.2.1.8
- HAMAP:   MF_01145
- InterPro:   IPR023059
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880

Pfam domain/function: PF00639 Rotamase; PF05698 Trigger_C

EC number: =5.2.1.8

Molecular weight: Translated: 32510; Mature: 32510

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS01096 PPIC_PPIASE_1; PS50198 PPIC_PPIASE_2; PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIAAITATSILALSACSSGDKEVIAKTDAGDVTKGELYTNMKKTAGASVLTQLVQE
CCCCEEHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KVLDKKYKVSDKEIDNKLKEYKTQLGDQYTALEKQYGKDYLKEQVKYELLTQKAAKDNIK
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VTDADIKEYWEGLKGKIRASHILVADKKTAEEVEKKLKKGEKFEDLAKEYSTDSSASKGG
EECHHHHHHHHHHCCCHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
DLGWFAKEGQMDETFSKAAFKLKTGEVSDPVKTQYGYHIIKKTEERGKYDDMKKELKSEV
CCCCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LEQKLNDNAAVQEAVQKVMKKADIEVKDKDLKDTFNTSSTSNSTSSSSSNSK
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIAIAAITATSILALSACSSGDKEVIAKTDAGDVTKGELYTNMKKTAGASVLTQLVQE
CCCCEEHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KVLDKKYKVSDKEIDNKLKEYKTQLGDQYTALEKQYGKDYLKEQVKYELLTQKAAKDNIK
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VTDADIKEYWEGLKGKIRASHILVADKKTAEEVEKKLKKGEKFEDLAKEYSTDSSASKGG
EECHHHHHHHHHHCCCHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
DLGWFAKEGQMDETFSKAAFKLKTGEVSDPVKTQYGYHIIKKTEERGKYDDMKKELKSEV
CCCCCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LEQKLNDNAAVQEAVQKVMKKADIEVKDKDLKDTFNTSSTSNSTSSSSSNSK
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1956302; 9579061; 9384377; 3145906