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The map label for this gene is sigM

Identifier: 16078017

GI number: 16078017

Start: 1029577

End: 1030068

Strand: Reverse

Name: sigM

Synonym: BSU09520

Alternate gene names: 16078017

Gene position: 1030068-1029577 (Counterclockwise)

Preceding gene: 16078020

Following gene: 16078016

Centisome position: 24.43

GC content: 39.63

Gene sequence:

>492_bases
GTGACGATCGATGAAATTTACCAAATGTACATGAATGATGTTTACAGGTTCCTGCTCTCCATGACAAAAGACAAGCATCT
TGCTGAAGACTTACTGCAGGAAACCTTTATGCGGGCATACATACACATTCACTCCTATGATCACAGCAAAGTAAAGCCCT
GGCTTTTTCAAGTGGCGCGAAACGCCTTCATTGATTACGTCAGAAAGCATAAGAAAGAAGTAACCATTTCTGATGACTTA
ATCGGAAGCCTCTTTCAAAATGCTGTTCAAAGTCCTGCCCATCAGGTAGAGATAAAGGAAGTGCTGACTGGTTATATGTC
CGAGCTCCCCGATAATTATCGGGAGGCCTTAACGCTATATTATTTAAAAGAGTTAAACTACAAAGAAGCATCCCATATTA
TGAATATTTCAGAGGCGAATTTTAAAAGTGTTTTATTTCGTGCCAGACAGCGGCTGAAAGCACTTTATAATAGAGGTGTT
AATGATGAATGA

Upstream 100 bases:

>100_bases
ATAGTAATGGTGTTTGATTAGGTATAAAAAAGCCGTTTGCATGTAATGTGCAACTTTAAACCTTTCTTATGCGTGTATAA
CATAGAGGGGAGAAAAGGCA

Downstream 100 bases:

>100_bases
AGAATTTAAAAAGCGTTTTGATCAATATAAAAATGGGGAAATGAGCGACCAGGAAATGACTGCATTTGAGGAAGAACTCG
AGAAGCTTGAAGTATATCAG

Product: RNA polymerase sigma factor SigM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVARNAFIDYVRKHKKEVTISDDL
IGSLFQNAVQSPAHQVEIKEVLTGYMSELPDNYREALTLYYLKELNYKEASHIMNISEANFKSVLFRARQRLKALYNRGV
NDE

Sequences:

>Translated_163_residues
MTIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVARNAFIDYVRKHKKEVTISDDL
IGSLFQNAVQSPAHQVEIKEVLTGYMSELPDNYREALTLYYLKELNYKEASHIMNISEANFKSVLFRARQRLKALYNRGV
NDE
>Mature_162_residues
TIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVARNAFIDYVRKHKKEVTISDDLI
GSLFQNAVQSPAHQVEIKEVLTGYMSELPDNYREALTLYYLKELNYKEASHIMNISEANFKSVLFRARQRLKALYNRGVN
DE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor may be involved in the maintenance of membrane and cell wall integrity in response to salt, acid and e

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): SIGM_BACSU (O07582)

Other databases:

- EMBL:   Y14082
- EMBL:   AL009126
- PIR:   C69826
- RefSeq:   NP_388833.1
- ProteinModelPortal:   O07582
- SMR:   O07582
- IntAct:   O07582
- EnsemblBacteria:   EBBACT00000003103
- GeneID:   936267
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09520
- NMPDR:   fig|224308.1.peg.952
- GenoList:   BSU09520
- GeneTree:   EBGT00070000031727
- HOGENOM:   HBG753452
- OMA:   PDNYREA
- ProtClustDB:   PRK09644
- BioCyc:   BSUB:BSU09520-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014296
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR02950
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 19407; Mature: 19276

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVAR
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NAFIDYVRKHKKEVTISDDLIGSLFQNAVQSPAHQVEIKEVLTGYMSELPDNYREALTLY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YLKELNYKEASHIMNISEANFKSVLFRARQRLKALYNRGVNDE
HHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVAR
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NAFIDYVRKHKKEVTISDDLIGSLFQNAVQSPAHQVEIKEVLTGYMSELPDNYREALTLY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YLKELNYKEASHIMNISEANFKSVLFRARQRLKALYNRGVNDE
HHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 12775685; 10216858