Click here to switch to the map view.

The map label for this gene is yfhQ

Identifier: 16077929

GI number: 16077929

Start: 935656

End: 936765

Strand: Direct

Name: yfhQ

Synonym: BSU08630

Alternate gene names: 16077929

Gene position: 935656-936765 (Clockwise)

Preceding gene: 255767195

Following gene: 16077931

Centisome position: 22.2

GC content: 45.77

Gene sequence:

>1110_bases
ATGAACGTACTTGAAGACAAATTAAAACAAAAAGACATACAGCAATTTCGCGATGATTTAATCTCATGGTTTGAACGAGA
GCAGCGCGTTTTGCCGTGGAGAGAGGACCAAGATCCATATAAGGTGTGGGTATCTGAAGTGATGCTGCAGCAAACGAGAG
TAGAGACAGTGATTCCTTACTTTCTCCGGTTTGTAGAACAATTCCCGACAGTGGAAGCGCTCGCTGATGCTGATGAAGAA
AAGGTGCTGAAAGCATGGGAAGGGCTCGGCTATTATTCGCGAGTCAGAAACCTGCAAAGCGCGGTAAAAGAAGTGAAACA
GGAATACGGCGGCATTGTTCCTCCAGACGAGAAAGATTTTGGCGGGTTAAAAGGCGTCGGCCCTTATACAAAGGGAGCGG
TGCTCAGCATTGCCTACAATAAACCCATTCCCGCGGTTGACGGAAATGTGATGCGTGTCATGTCCAGGATTCTATCTATT
TGGGATGATATTGCCAAACCAAAAACAAGAACGATCTTTGAAGACGCCATACGAGCTTTTATTTCCAAAGAAAAACCATC
TGAATTTAATCAGGGCTTGATGGAGCTTGGAGCCTTGATCTGTACGCCAAAATCACCCTCATGCCTGCTTTGCCCGGTTC
AGCAGCATTGCTCAGCATTTGAAGAAGGCACTGAACGGGAGCTTCCGGTAAAAAGCAAAAAGAAAAAGCCCGGGATCAAA
ACCATGGCCGCGATCGTACTGACTGATGAAGACGGACAAGTGTATATCCACAAACGGCCGTCTAAAGGTTTGCTCGCCAA
CCTGTGGGAATTCCCGAATCTTGAAACACAAAAAGGGATCAAAACTGAGCGTGAACAGCTCATTGCTTTCTTAGAAAACG
AATATGGCATACAAGCTGATATCAGTGATCTGCAAGGTGTAGTCGAGCATGTTTTCACCCACCTGGTATGGAATATTTCA
GTGTTTTTCGGTAAAGTAAAACAAGTGTCGGATACCAGCAAGCTGAAAAAAGTAACGAAAGAAGAGCTCGAACAATTTGC
GTTCCCGGTGTCACATCAAAAAATCTGGAAGATGGCAGGAGAAGCAGCCGCCATCTCGGCTGCTCCGTAA

Upstream 100 bases:

>100_bases
ATTACTATTTAATGTAACATATTATCGTACTGTGCCCTTAGTGTACAACAAAAAACAGTCTAAAGAAAAGCGGGGCGCGA
AAGACTGGGGAGAAACACAT

Downstream 100 bases:

>100_bases
ACCATTCTTAATCGTAAGAGACGCGCGTGCCGTGGCTGTAGTCCGCTTCATTGCGCTTCATTCCGCCGCGCGCTTCAATT
TCTTGCGTTAATTCCCGGTA

Product: A/G-specific adenine glycosylase or DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNVLEDKLKQKDIQQFRDDLISWFEREQRVLPWREDQDPYKVWVSEVMLQQTRVETVIPYFLRFVEQFPTVEALADADEE
KVLKAWEGLGYYSRVRNLQSAVKEVKQEYGGIVPPDEKDFGGLKGVGPYTKGAVLSIAYNKPIPAVDGNVMRVMSRILSI
WDDIAKPKTRTIFEDAIRAFISKEKPSEFNQGLMELGALICTPKSPSCLLCPVQQHCSAFEEGTERELPVKSKKKKPGIK
TMAAIVLTDEDGQVYIHKRPSKGLLANLWEFPNLETQKGIKTEREQLIAFLENEYGIQADISDLQGVVEHVFTHLVWNIS
VFFGKVKQVSDTSKLKKVTKEELEQFAFPVSHQKIWKMAGEAAAISAAP

Sequences:

>Translated_369_residues
MNVLEDKLKQKDIQQFRDDLISWFEREQRVLPWREDQDPYKVWVSEVMLQQTRVETVIPYFLRFVEQFPTVEALADADEE
KVLKAWEGLGYYSRVRNLQSAVKEVKQEYGGIVPPDEKDFGGLKGVGPYTKGAVLSIAYNKPIPAVDGNVMRVMSRILSI
WDDIAKPKTRTIFEDAIRAFISKEKPSEFNQGLMELGALICTPKSPSCLLCPVQQHCSAFEEGTERELPVKSKKKKPGIK
TMAAIVLTDEDGQVYIHKRPSKGLLANLWEFPNLETQKGIKTEREQLIAFLENEYGIQADISDLQGVVEHVFTHLVWNIS
VFFGKVKQVSDTSKLKKVTKEELEQFAFPVSHQKIWKMAGEAAAISAAP
>Mature_369_residues
MNVLEDKLKQKDIQQFRDDLISWFEREQRVLPWREDQDPYKVWVSEVMLQQTRVETVIPYFLRFVEQFPTVEALADADEE
KVLKAWEGLGYYSRVRNLQSAVKEVKQEYGGIVPPDEKDFGGLKGVGPYTKGAVLSIAYNKPIPAVDGNVMRVMSRILSI
WDDIAKPKTRTIFEDAIRAFISKEKPSEFNQGLMELGALICTPKSPSCLLCPVQQHCSAFEEGTERELPVKSKKKKPGIK
TMAAIVLTDEDGQVYIHKRPSKGLLANLWEFPNLETQKGIKTEREQLIAFLENEYGIQADISDLQGVVEHVFTHLVWNIS
VFFGKVKQVSDTSKLKKVTKEELEQFAFPVSHQKIWKMAGEAAAISAAP

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8- oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal effici

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HhH domain

Homologues:

Organism=Homo sapiens, GI115298648, Length=405, Percent_Identity=31.8518518518519, Blast_Score=208, Evalue=8e-54,
Organism=Homo sapiens, GI190358497, Length=405, Percent_Identity=31.8518518518519, Blast_Score=208, Evalue=8e-54,
Organism=Homo sapiens, GI6912520, Length=405, Percent_Identity=31.8518518518519, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI115298654, Length=405, Percent_Identity=31.8518518518519, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI115298652, Length=405, Percent_Identity=31.8518518518519, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI115298650, Length=405, Percent_Identity=31.8518518518519, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1789331, Length=310, Percent_Identity=37.741935483871, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFHQ_BACSU (O31584)

Other databases:

- EMBL:   D85082
- EMBL:   AL009126
- PIR:   A69802
- RefSeq:   NP_388743.1
- HSSP:   P17802
- ProteinModelPortal:   O31584
- SMR:   O31584
- EnsemblBacteria:   EBBACT00000002377
- GeneID:   939222
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08630
- NMPDR:   fig|224308.1.peg.862
- GenoList:   BSU08630
- GeneTree:   EBGT00050000000495
- HOGENOM:   HBG464473
- OMA:   FHLDIQP
- PhylomeDB:   O31584
- ProtClustDB:   CLSK2393372
- BioCyc:   BSUB:BSU08630-MONOMER
- GO:   GO:0005622
- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- Gene3D:   G3DSA:3.90.79.10
- SMART:   SM00478
- SMART:   SM00525
- TIGRFAMs:   TIGR01084

Pfam domain/function: PF00730 HhH-GPD; SSF48150 DNA_glycsylse; SSF55811 NUDIX_hydrolase

EC number: 3.2.2.-

Molecular weight: Translated: 41964; Mature: 41964

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00764 ENDONUCLEASE_III_1; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLEDKLKQKDIQQFRDDLISWFEREQRVLPWREDQDPYKVWVSEVMLQQTRVETVIPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLRFVEQFPTVEALADADEEKVLKAWEGLGYYSRVRNLQSAVKEVKQEYGGIVPPDEKDF
HHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GGLKGVGPYTKGAVLSIAYNKPIPAVDGNVMRVMSRILSIWDDIAKPKTRTIFEDAIRAF
CCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ISKEKPSEFNQGLMELGALICTPKSPSCLLCPVQQHCSAFEEGTERELPVKSKKKKPGIK
HCCCCCCHHHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCHH
TMAAIVLTDEDGQVYIHKRPSKGLLANLWEFPNLETQKGIKTEREQLIAFLENEYGIQAD
HEEEEEEECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ISDLQGVVEHVFTHLVWNISVFFGKVKQVSDTSKLKKVTKEELEQFAFPVSHQKIWKMAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
EAAAISAAP
CCCCCCCCC
>Mature Secondary Structure
MNVLEDKLKQKDIQQFRDDLISWFEREQRVLPWREDQDPYKVWVSEVMLQQTRVETVIPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLRFVEQFPTVEALADADEEKVLKAWEGLGYYSRVRNLQSAVKEVKQEYGGIVPPDEKDF
HHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GGLKGVGPYTKGAVLSIAYNKPIPAVDGNVMRVMSRILSIWDDIAKPKTRTIFEDAIRAF
CCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ISKEKPSEFNQGLMELGALICTPKSPSCLLCPVQQHCSAFEEGTERELPVKSKKKKPGIK
HCCCCCCHHHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCHH
TMAAIVLTDEDGQVYIHKRPSKGLLANLWEFPNLETQKGIKTEREQLIAFLENEYGIQAD
HEEEEEEECCCCEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ISDLQGVVEHVFTHLVWNISVFFGKVKQVSDTSKLKKVTKEELEQFAFPVSHQKIWKMAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
EAAAISAAP
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377