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The map label for this gene is yfjO

Identifier: 16077869

GI number: 16077869

Start: 873402

End: 874802

Strand: Direct

Name: yfjO

Synonym: BSU08020

Alternate gene names: 16077869

Gene position: 873402-874802 (Clockwise)

Preceding gene: 16077868

Following gene: 255767191

Centisome position: 20.72

GC content: 49.68

Gene sequence:

>1401_bases
GTGAACCAACAAAAGAAACAGGCGCCCGTCGAACTGAAGGTCGGACAAACTTTTCCGCTGACGATTAAGCGTCTCGGCAT
TAACGGAGAAGGTGTTGGCTATTTTAAGAAAAAAGTCGTCTTCGTGCCCGGCGCGCTTCCTGGCGAAGAAGTTGTTGTGC
AGGCCACAAAGGTCCAGCCGAAGTTTTCGGAGGGCCGCATTAAGAAAATCCGCAAGGCATCTGAGCACCGGGTGGCACCG
CCTTGCCCTGTTTATGAACAATGCGGCGGCTGTCAGCTTCAGCACCTTGCATACAGCCAGCAGCTTCGGGAAAAACGTGA
TATCGTCATTCAGTCTCTTGAACGCCATACGAAGTTTAAAGTTGAAAACATGGAAATCAAAGAAACGATTGGCATGGACA
ACCCGTGGAATTACCGCAATAAAAGCCAATTCCAGATCGGGCGCTCGCAAAGCGGCAGCATCATCGCCGGCTTATACGGC
CTTGATTCTCATGATATCGTGCCGATTAAAGACTGTATCGTTCAGCATCCGGCAACGAACAAAACAACAGGCATCGTCCG
CCGCATTTTAGAGGACTTCAATGTGTCTGTTTATAATGAAAGAAAACGAAAAGGCGACGTTCGCACGATCGTGACGAGAG
TCGGCTTTGAAACAGGCGAAGTGCAGGTCGTGCTTGTAACCGCAAAAGAAACGCTTCCGCACAAGGAAGAAATCGTCAAA
GCGATTCAAAAGCGATTGCCAGAGGTCAAATCCATTATTCAGAATGTAAACGGGGCGAAAACCTCAGTGATTTTTGGTGA
GAAAACAAAACAGCTCGCCGGAAAAACAGTGATTCAAGAAGTGCTCGGCGACGTATCTTTTGAATTGAGCGCCCGCGCTT
TCTTCCAGCTCAATCCGGAACAGACCGTCAAGCTGTATGATGAAGTGAAAAAAGCGGCGCAGCTGACCGGAAAAGAAAAA
GTCGTTGATGCGTATTGCGGCGTCGGCACTATCGGCATGTGGGTTGCGGACGGAGCGAAGGAAGTCCGCGGCATGGACGT
CATCAAGGAATCAATTGACGATGCTAAGAAAAACGCCAAAAAACACGGCATGGCAAATGCCACATACGTAACAGGAACAG
CCGAGCACTGGCTCCCGAAATGGACGAAAGAAGGCTTCCGCCCGGACGTCGTCATTGTAGACCCGCCAAGAACGGGCTGT
GACAGCACGTTCTTGGACACGATCAAAAAAGTCAAACCGAAACGCTTTGTCTACGTTTCCTGCAATCCATCCACGCTTGC
GAAAGATTTGCAGACGCTGTCGAAGGACTATCGCGTGGACTATATTCAGCCTGTCGATATGTTTCCGCAGACGGCGCATG
TTGAAGCAGTGGCGAGGCTTGTATTAAAATCGTCTAACTAA

Upstream 100 bases:

>100_bases
GAAGGAAATCCTGCAAACGTGTTATACTAGAAAGATGTGAAAAAACCATTTCACTTTCTGCCATCCGCAGAAAGCTTCAT
ATAGAAACGGAGAGAACAAC

Downstream 100 bases:

>100_bases
AAAGAACATTTCCCAGTCATAAAATAGTTTTCCTAATAAGACCTGGATTTCGGTAAAATAAACAATTCCGATTTCCGGGT
CTTTTTCGTGCGCAGCTCTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNQQKKQAPVELKVGQTFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVQATKVQPKFSEGRIKKIRKASEHRVAP
PCPVYEQCGGCQLQHLAYSQQLREKRDIVIQSLERHTKFKVENMEIKETIGMDNPWNYRNKSQFQIGRSQSGSIIAGLYG
LDSHDIVPIKDCIVQHPATNKTTGIVRRILEDFNVSVYNERKRKGDVRTIVTRVGFETGEVQVVLVTAKETLPHKEEIVK
AIQKRLPEVKSIIQNVNGAKTSVIFGEKTKQLAGKTVIQEVLGDVSFELSARAFFQLNPEQTVKLYDEVKKAAQLTGKEK
VVDAYCGVGTIGMWVADGAKEVRGMDVIKESIDDAKKNAKKHGMANATYVTGTAEHWLPKWTKEGFRPDVVIVDPPRTGC
DSTFLDTIKKVKPKRFVYVSCNPSTLAKDLQTLSKDYRVDYIQPVDMFPQTAHVEAVARLVLKSSN

Sequences:

>Translated_466_residues
MNQQKKQAPVELKVGQTFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVQATKVQPKFSEGRIKKIRKASEHRVAP
PCPVYEQCGGCQLQHLAYSQQLREKRDIVIQSLERHTKFKVENMEIKETIGMDNPWNYRNKSQFQIGRSQSGSIIAGLYG
LDSHDIVPIKDCIVQHPATNKTTGIVRRILEDFNVSVYNERKRKGDVRTIVTRVGFETGEVQVVLVTAKETLPHKEEIVK
AIQKRLPEVKSIIQNVNGAKTSVIFGEKTKQLAGKTVIQEVLGDVSFELSARAFFQLNPEQTVKLYDEVKKAAQLTGKEK
VVDAYCGVGTIGMWVADGAKEVRGMDVIKESIDDAKKNAKKHGMANATYVTGTAEHWLPKWTKEGFRPDVVIVDPPRTGC
DSTFLDTIKKVKPKRFVYVSCNPSTLAKDLQTLSKDYRVDYIQPVDMFPQTAHVEAVARLVLKSSN
>Mature_466_residues
MNQQKKQAPVELKVGQTFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVQATKVQPKFSEGRIKKIRKASEHRVAP
PCPVYEQCGGCQLQHLAYSQQLREKRDIVIQSLERHTKFKVENMEIKETIGMDNPWNYRNKSQFQIGRSQSGSIIAGLYG
LDSHDIVPIKDCIVQHPATNKTTGIVRRILEDFNVSVYNERKRKGDVRTIVTRVGFETGEVQVVLVTAKETLPHKEEIVK
AIQKRLPEVKSIIQNVNGAKTSVIFGEKTKQLAGKTVIQEVLGDVSFELSARAFFQLNPEQTVKLYDEVKKAAQLTGKEK
VVDAYCGVGTIGMWVADGAKEVRGMDVIKESIDDAKKNAKKHGMANATYVTGTAEHWLPKWTKEGFRPDVVIVDPPRTGC
DSTFLDTIKKVKPKRFVYVSCNPSTLAKDLQTLSKDYRVDYIQPVDMFPQTAHVEAVARLVLKSSN

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=209, Percent_Identity=33.0143540669856, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI51173878, Length=209, Percent_Identity=33.0143540669856, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI269784680, Length=258, Percent_Identity=28.2945736434109, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI269784676, Length=258, Percent_Identity=28.2945736434109, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI20149688, Length=258, Percent_Identity=28.2945736434109, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI269784678, Length=258, Percent_Identity=28.2945736434109, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=26.457399103139, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1787083, Length=328, Percent_Identity=25.609756097561, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790403, Length=160, Percent_Identity=30, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=163, Percent_Identity=29.4478527607362, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322909, Length=436, Percent_Identity=25.6880733944954, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24666579, Length=415, Percent_Identity=25.5421686746988, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFJO_BACSU (O31545)

Other databases:

- EMBL:   D78509
- EMBL:   AL009126
- PIR:   F69806
- RefSeq:   NP_388683.1
- ProteinModelPortal:   O31545
- SMR:   O31545
- EnsemblBacteria:   EBBACT00000002746
- GeneID:   936146
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08020
- NMPDR:   fig|224308.1.peg.802
- GenoList:   BSU08020
- GeneTree:   EBGT00050000002272
- HOGENOM:   HBG690163
- OMA:   RTVMIRR
- PhylomeDB:   O31545
- ProtClustDB:   CLSK2752340
- BioCyc:   BSUB:BSU08020-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 52133; Mature: 52133

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 421-421 BINDING 296-296 BINDING 325-325 BINDING 346-346 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQKKQAPVELKVGQTFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVQATKVQP
CCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCC
KFSEGRIKKIRKASEHRVAPPCPVYEQCGGCQLQHLAYSQQLREKRDIVIQSLERHTKFK
CCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VENMEIKETIGMDNPWNYRNKSQFQIGRSQSGSIIAGLYGLDSHDIVPIKDCIVQHPATN
ECCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCEEHHHHHHCCCCCC
KTTGIVRRILEDFNVSVYNERKRKGDVRTIVTRVGFETGEVQVVLVTAKETLPHKEEIVK
CHHHHHHHHHHHCCCEEECCHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
AIQKRLPEVKSIIQNVNGAKTSVIFGEKTKQLAGKTVIQEVLGDVSFELSARAFFQLNPE
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEECEEEEEEECHH
QTVKLYDEVKKAAQLTGKEKVVDAYCGVGTIGMWVADGAKEVRGMDVIKESIDDAKKNAK
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KHGMANATYVTGTAEHWLPKWTKEGFRPDVVIVDPPRTGCDSTFLDTIKKVKPKRFVYVS
HCCCCCCEEEECCHHHHCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEE
CNPSTLAKDLQTLSKDYRVDYIQPVDMFPQTAHVEAVARLVLKSSN
CCHHHHHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQQKKQAPVELKVGQTFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVQATKVQP
CCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCC
KFSEGRIKKIRKASEHRVAPPCPVYEQCGGCQLQHLAYSQQLREKRDIVIQSLERHTKFK
CCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VENMEIKETIGMDNPWNYRNKSQFQIGRSQSGSIIAGLYGLDSHDIVPIKDCIVQHPATN
ECCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCEEHHHHHHCCCCCC
KTTGIVRRILEDFNVSVYNERKRKGDVRTIVTRVGFETGEVQVVLVTAKETLPHKEEIVK
CHHHHHHHHHHHCCCEEECCHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
AIQKRLPEVKSIIQNVNGAKTSVIFGEKTKQLAGKTVIQEVLGDVSFELSARAFFQLNPE
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEECEEEEEEECHH
QTVKLYDEVKKAAQLTGKEKVVDAYCGVGTIGMWVADGAKEVRGMDVIKESIDDAKKNAK
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KHGMANATYVTGTAEHWLPKWTKEGFRPDVVIVDPPRTGCDSTFLDTIKKVKPKRFVYVS
HCCCCCCEEEECCHHHHCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEE
CNPSTLAKDLQTLSKDYRVDYIQPVDMFPQTAHVEAVARLVLKSSN
CCHHHHHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377