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The map label for this gene is yfjP

Identifier: 16077868

GI number: 16077868

Start: 872425

End: 873288

Strand: Direct

Name: yfjP

Synonym: BSU08010

Alternate gene names: 16077868

Gene position: 872425-873288 (Clockwise)

Preceding gene: 16077865

Following gene: 16077869

Centisome position: 20.7

GC content: 49.31

Gene sequence:

>864_bases
ATGTGGAAGGAAAAAGTATCTGTCACGCCTCCGTATCATTTTGACCGCGTGCTGGACAGGCTGTCTTTAGACCCCCTCAA
TGCTGTTGATCGAGAGGCGAGGGAAGTCCGTGTGCCAATCAGAAATCAAGCGGGGGACGTATGTATTGTAAAGGTACAGG
CGTTAGGCCATGCGGGCGAGCCTGAGTTCCTCGTCAGCGGGGAAACGGATCAGGGAGAAATGATGAAGGAAATCAAGCGG
ATTTTTCAATGGGAAAATCATTTGCAGCACGTTCTGGATCATTTTTCAAAAACGAGCCTGTCCGCCATTTTTGAAGAGCA
TGCCGGGACGCCGCTTGTTCTGGACTACAGCGTGTACAACTGCATGATGAAATGCATTATCCACCAGCAGCTTAATCTTT
CCTTCGCTTACACGCTAACAGAACGGTTTGTTCATGCGTTTGGCGAGCAGAAGGATGGCGTGTGGTGCTATCCAAAGCCG
GAAACGATTGCGGAACTCGATTATCAGGATCTGCGTGATCTGCAATTCAGCATGAGAAAAGCGGAATACACCATTGATAC
ATCAAGGATGATCGCAGAAGGCACACTCAGCTTGTCAGAGCTGCCGCATATGGCGGATGAAGACATTATGAAGAAACTGA
TCAAAATCAGGGGTATCGGCCCGTGGACCGTGCAAAACGTCCTCATGTTCGGACTTGGCCGTCCCAATTTATTCCCGCTT
GCCGATATCGGCCTCCAGAATGCCATCAAACGCCACTTTCAGCTGGACGATAAGCCGGCAAAGGATGTCATGCTGGCGAT
GAGCAAGGAGTGGGAGCCTTATTTAAGCTATGCGTCTTTGTATTTGTGGAGGAGTATAGAATAA

Upstream 100 bases:

>100_bases
CATTATATCATGCGGTCTTTATGAGAAAAGTATACGGTACTCTCTGCCTAATTCCAGCGGCATGTTATACTGAACAAAAA
CGGAAAAAAGGGTGGATCTC

Downstream 100 bases:

>100_bases
TTCGTCTGACAATGAAGGAAATCCTGCAAACGTGTTATACTAGAAAGATGTGAAAAAACCATTTCACTTTCTGCCATCCG
CAGAAAGCTTCATATAGAAA

Product: DNA-modified purine glycosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MWKEKVSVTPPYHFDRVLDRLSLDPLNAVDREAREVRVPIRNQAGDVCIVKVQALGHAGEPEFLVSGETDQGEMMKEIKR
IFQWENHLQHVLDHFSKTSLSAIFEEHAGTPLVLDYSVYNCMMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKP
ETIAELDYQDLRDLQFSMRKAEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPL
ADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE

Sequences:

>Translated_287_residues
MWKEKVSVTPPYHFDRVLDRLSLDPLNAVDREAREVRVPIRNQAGDVCIVKVQALGHAGEPEFLVSGETDQGEMMKEIKR
IFQWENHLQHVLDHFSKTSLSAIFEEHAGTPLVLDYSVYNCMMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKP
ETIAELDYQDLRDLQFSMRKAEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPL
ADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE
>Mature_287_residues
MWKEKVSVTPPYHFDRVLDRLSLDPLNAVDREAREVRVPIRNQAGDVCIVKVQALGHAGEPEFLVSGETDQGEMMKEIKR
IFQWENHLQHVLDHFSKTSLSAIFEEHAGTPLVLDYSVYNCMMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKP
ETIAELDYQDLRDLQFSMRKAEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPL
ADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family

Homologues:

Organism=Escherichia coli, GI1788383, Length=219, Percent_Identity=27.3972602739726, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFJP_BACSU (O31544)

Other databases:

- EMBL:   D78509
- EMBL:   AL009126
- PIR:   G69806
- RefSeq:   NP_388682.1
- ProteinModelPortal:   O31544
- SMR:   O31544
- EnsemblBacteria:   EBBACT00000003204
- GeneID:   939192
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08010
- NMPDR:   fig|224308.1.peg.801
- GenoList:   BSU08010
- GeneTree:   EBGT00050000002395
- HOGENOM:   HBG364629
- OMA:   QLLPIRG
- PhylomeDB:   O31544
- ProtClustDB:   CLSK2460897
- BioCyc:   BSUB:BSU08010-MONOMER
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00278

Pfam domain/function: PF00730 HhH-GPD; SSF48150 DNA_glycsylse

EC number: =3.2.2.21

Molecular weight: Translated: 33179; Mature: 33179

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: ACT_SITE 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKEKVSVTPPYHFDRVLDRLSLDPLNAVDREAREVRVPIRNQAGDVCIVKVQALGHAGE
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCEEEEEEEECCCCCC
PEFLVSGETDQGEMMKEIKRIFQWENHLQHVLDHFSKTSLSAIFEEHAGTPLVLDYSVYN
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
CMMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKPETIAELDYQDLRDLQFSMRK
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHCCHHHHHHHHHHHHH
AEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPL
HHEEECHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
ADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWKEKVSVTPPYHFDRVLDRLSLDPLNAVDREAREVRVPIRNQAGDVCIVKVQALGHAGE
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCEEEEEEEECCCCCC
PEFLVSGETDQGEMMKEIKRIFQWENHLQHVLDHFSKTSLSAIFEEHAGTPLVLDYSVYN
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
CMMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKPETIAELDYQDLRDLQFSMRK
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHCCHHHHHHHHHHHHH
AEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPL
HHEEECHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
ADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377