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The map label for this gene is yfkJ

Identifier: 16077855

GI number: 16077855

Start: 862004

End: 862474

Strand: Direct

Name: yfkJ

Synonym: BSU07880

Alternate gene names: 16077855

Gene position: 862004-862474 (Clockwise)

Preceding gene: 16077852

Following gene: 16077856

Centisome position: 20.45

GC content: 46.92

Gene sequence:

>471_bases
ATGATAAGCGTGTTATTTGTTTGTTTAGGTAACATTTGCCGGTCTCCGATGGCGGAAGCGATTTTTAGGGACCTGGCAGC
CAAAAAAGGATTAGAGGGGAAAATCAAGGCGGACTCAGCCGGTATTGGCGGCTGGCACATCGGCAACCCCCCGCATGAGG
GAACGCAGGAGATATTGCGCAGAGAAGGAATCAGCTTTGATGGCATGCTGGCGCGTCAAGTCAGCGAACAGGATCTGGAC
GATTTTGATTACATCATTGCAATGGATGCAGAAAATATCGGAAGTCTCAGAAGCATGGCGGGTTTTAAAAACACCTCGCA
TATCAAAAGGCTCTTGGACTATGTTGAAGATTCAGATCTGGCTGACGTACCCGATCCTTACTACACAGGGAACTTTGAAG
AGGTCTGCCAATTAATCAAAACGGGCTGTGAGCAGTTGCTTGCATCCATTCAAAAAGAAAAACAATTGTGA

Upstream 100 bases:

>100_bases
GTGAAGACATCAGTTGAAAAGAAAATGAACATCCTACTAAGATATTCATGAAGGTTTCTTTTTAGAGAAATAGGGGCAAA
GAATAGGGAGGTTGCACATC

Downstream 100 bases:

>100_bases
GTGAAAGGAGAATTTGCATGAACAACGAACGTTTAATGCTGAAAGGGATATTTCTCGGAGCTGCGGCAGGCGCGGCGTTA
TCACTGCTCCATAAGCCGAC

Product: protein-tyrosine-phosphatase

Products: NA

Alternate protein names: LMPTP

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLD
DFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDLADVPDPYYTGNFEEVCQLIKTGCEQLLASIQKEKQL

Sequences:

>Translated_156_residues
MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLD
DFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDLADVPDPYYTGNFEEVCQLIKTGCEQLLASIQKEKQL
>Mature_156_residues
MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLD
DFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDLADVPDPYYTGNFEEVCQLIKTGCEQLLASIQKEKQL

Specific function: Dephosphorylates the phosphotyrosine-containing proteins. Involved in ethanol stress resistance

COG id: COG0394

COG function: function code T; Protein-tyrosine-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family

Homologues:

Organism=Homo sapiens, GI4757714, Length=148, Percent_Identity=39.8648648648649, Blast_Score=107, Evalue=5e-24,
Organism=Homo sapiens, GI6005988, Length=148, Percent_Identity=37.1621621621622, Blast_Score=96, Evalue=1e-20,
Organism=Homo sapiens, GI96304457, Length=77, Percent_Identity=50.6493506493506, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87081812, Length=145, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325330, Length=150, Percent_Identity=34, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI78706770, Length=148, Percent_Identity=41.2162162162162, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI78706772, Length=148, Percent_Identity=41.2162162162162, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI78711846, Length=152, Percent_Identity=38.8157894736842, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI78706766, Length=155, Percent_Identity=34.8387096774194, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78706768, Length=155, Percent_Identity=34.8387096774194, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78706764, Length=130, Percent_Identity=36.9230769230769, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648069, Length=86, Percent_Identity=43.0232558139535, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFKJ_BACSU (O35016)

Other databases:

- EMBL:   AL009126
- EMBL:   D83967
- PIR:   E69808
- RefSeq:   NP_388669.1
- ProteinModelPortal:   O35016
- SMR:   O35016
- PptaseDB:   P3D0507171
- EnsemblBacteria:   EBBACT00000003915
- GeneID:   939191
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07880
- NMPDR:   fig|224308.1.peg.788
- GenoList:   BSU07880
- GeneTree:   EBGT00050000002758
- HOGENOM:   HBG730979
- OMA:   VDSAGTH
- PhylomeDB:   O35016
- ProtClustDB:   CLSK2502625
- BioCyc:   BSUB:BSU07880-MONOMER
- BRENDA:   3.1.3.48
- InterPro:   IPR000106
- InterPro:   IPR017867
- PANTHER:   PTHR11717
- PRINTS:   PR00719
- SMART:   SM00226

Pfam domain/function: PF01451 LMWPc; SSF52788 Tyr_Pase_low_mol_wt

EC number: =3.1.3.48

Molecular weight: Translated: 17269; Mature: 17269

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 14-14 ACT_SITE 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILR
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
REGISFDGMLARQVSEQDLDDFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDL
HCCCCHHHHHHHHHHHCCCCCHHEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
ADVPDPYYTGNFEEVCQLIKTGCEQLLASIQKEKQL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILR
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
REGISFDGMLARQVSEQDLDDFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDL
HCCCCHHHHHHHHHHHCCCCCHHEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
ADVPDPYYTGNFEEVCQLIKTGCEQLLASIQKEKQL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377