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The map label for this gene is yfkO

Identifier: 16077850

GI number: 16077850

Start: 854412

End: 855077

Strand: Direct

Name: yfkO

Synonym: BSU07830

Alternate gene names: 16077850

Gene position: 854412-855077 (Clockwise)

Preceding gene: 16077849

Following gene: 16077852

Centisome position: 20.27

GC content: 44.44

Gene sequence:

>666_bases
ATGGCAGATCTAAAGACACAAATTCTGGATGCATACAATTTCAGGCATGCGACTAAGGAATTTGACCCGAATAAAAAAGT
GTCAGACAGCGATTTTGAATTTATTTTAGAAACAGGAAGACTGTCTCCGAGCTCACTCGGCCTTGAGCCTTGGAAATTTG
TCGTCGTCCAAAATCCGGAATTCCGCGAGAAGCTTCGTGAATACACATGGGGCGCGCAAAAGCAGCTGCCGACCGCAAGC
CATTTCGTCCTGATTCTTGCGCGTACAGCGAAGGATATCAAATATAATGCGGACTACATCAAACGTCATTTAAAAGAAGT
AAAACAAATGCCTCAAGACGTGTACGAAGGCTATTTAAGCAAAACAGAAGAATTCCAGAAAAACGATTTGCATTTGCTTG
AAAGTGACAGAACATTGTTTGACTGGGCATCAAAACAAACCTATATCGCGCTTGGCAACATGATGACTGCCGCTGCGCAA
ATCGGTGTTGATTCCTGCCCGATCGAAGGCTTCCAGTACGATCACATTCACCGTATTCTTGAAGAAGAAGGCCTTCTGGA
AAACGGAAGCTTCGATATTTCTGTGATGGTGGCATTCGGCTACCGAGTGAGAGACCCTCGTCCGAAAACAAGATCTGCTG
TTGAAGATGTTGTGAAGTGGGTTTAA

Upstream 100 bases:

>100_bases
TATCAAAATTGATACTATCCAACAAAAATGTGCGTACTTTTACTTTTTATCATAATGGCTACAATAGAGATGAGAGTAAT
CTAAAGGAGAGGTGTTTTAC

Downstream 100 bases:

>100_bases
AAGAAGAAAGCTGCTCGCATAGCGAGCAGCTCTTTTTTATGCCTGATTCGCTCTATTCTGTTTTCTCCTCTGATGTAAGT
ACATTGCCGTTCCGAGTGCG

Product: NAD(P)H-flavin oxidoreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTAS
HFVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLSKTEEFQKNDLHLLESDRTLFDWASKQTYIALGNMMTAAAQ
IGVDSCPIEGFQYDHIHRILEEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV

Sequences:

>Translated_221_residues
MADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTAS
HFVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLSKTEEFQKNDLHLLESDRTLFDWASKQTYIALGNMMTAAAQ
IGVDSCPIEGFQYDHIHRILEEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV
>Mature_220_residues
ADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASH
FVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLSKTEEFQKNDLHLLESDRTLFDWASKQTYIALGNMMTAAAQI
GVDSCPIEGFQYDHIHRILEEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family

Homologues:

Organism=Escherichia coli, GI1786792, Length=217, Percent_Identity=29.9539170506912, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YFKO_BACSU (O34475)

Other databases:

- EMBL:   D83967
- EMBL:   AL009126
- PIR:   B69809
- RefSeq:   NP_388664.1
- ProteinModelPortal:   O34475
- SMR:   O34475
- EnsemblBacteria:   EBBACT00000002472
- GeneID:   939189
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07830
- NMPDR:   fig|224308.1.peg.783
- GenoList:   BSU07830
- GeneTree:   EBGT00070000032048
- HOGENOM:   HBG751302
- OMA:   MMTSAAQ
- PhylomeDB:   O34475
- ProtClustDB:   CLSK886868
- BioCyc:   BSUB:BSU07830-MONOMER
- InterPro:   IPR000415

Pfam domain/function: PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25628; Mature: 25497

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: BINDING 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCH
FREKLREYTWGAQKQLPTASHFVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLS
HHHHHHHHCCCCHHCCCCHHHHHHHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KTEEFQKNDLHLLESDRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYDHIHRIL
HHHHHHHCCCEEECCCCHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV
HHCCCCCCCCCCEEEEEEECEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCH
FREKLREYTWGAQKQLPTASHFVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLS
HHHHHHHHCCCCHHCCCCHHHHHHHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KTEEFQKNDLHLLESDRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYDHIHRIL
HHHHHHHCCCEEECCCCHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV
HHCCCCCCCCCCEEEEEEECEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377