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The map label for this gene is ydgI

Identifier: 16077633

GI number: 16077633

Start: 612191

End: 612820

Strand: Reverse

Name: ydgI

Synonym: BSU05660

Alternate gene names: 16077633

Gene position: 612820-612191 (Counterclockwise)

Preceding gene: 16077634

Following gene: 16077630

Centisome position: 14.54

GC content: 45.87

Gene sequence:

>630_bases
ATGATCAAAACAAACGATTTTATGGAAATTATGAAAGGCCGCCGTTCTATCCGCAACTATGATCCGGCAGTAAAAATCAG
CAAAGAAGAAATGACAGAGATCTTAGAGGAAGCAACAACTGCCCCATCTTCTGTTAACGCGCAGCCATGGCGTTTTCTTG
TCATTGACAGCCCGGAAGGAAAAGAAAAGCTCGCACCGCTTGCAAGCTTTAACCAAACACAAGTCACAACATCATCTGCT
GTCATCGCTGTATTTGCAGACATGAACAACGCAGACTATCTAGAAGAAATCTATTCAAAAGCCGTGGAACTTGGTTACAT
GCCGCAGGAGGTCAAAGACAGACAAATCGCCGCGCTGACCGCACATTTTGAAAAGCTTCCGGCACAGGTCAACCGTGAAA
CGATCCTGATTGACGGAGGTCTTGTTTCCATGCAGCTGATGCTGACTGCACGCGCGCATGGCTACGATACAAACCCGATC
GGCGGATACGATAAAGAAAACATCGCGGAAACCTTCGGATTAGATAAAGAACGTTATGTACCGGTTATGCTACTTTCTAT
CGGAAAAGCAGCAGACGAAGGCTATGCTTCCTACCGTCTGCCGATTGATACAATTGCAGAATGGAAATAA

Upstream 100 bases:

>100_bases
CGCAAATGTAAGCGCAGAAGAACGCCGCCTGCTCATTGATGTGCTGGCGCGCATGCGAAACAATATAAATAATATCGAAG
CTTAAGAGGAGTGTATCAAC

Downstream 100 bases:

>100_bases
AACAAAGAGCCCGGGAATGTTTTCCGGGCTCTTTGTTTTTAAGTACAGCCGCCGTTTGATCGTCTGATCTTTTTGGGTAA
CACGTCTGAAGTTAGTCGTT

Product: dehydrogenase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIKTNDFMEIMKGRRSIRNYDPAVKISKEEMTEILEEATTAPSSVNAQPWRFLVIDSPEGKEKLAPLASFNQTQVTTSSA
VIAVFADMNNADYLEEIYSKAVELGYMPQEVKDRQIAALTAHFEKLPAQVNRETILIDGGLVSMQLMLTARAHGYDTNPI
GGYDKENIAETFGLDKERYVPVMLLSIGKAADEGYASYRLPIDTIAEWK

Sequences:

>Translated_209_residues
MIKTNDFMEIMKGRRSIRNYDPAVKISKEEMTEILEEATTAPSSVNAQPWRFLVIDSPEGKEKLAPLASFNQTQVTTSSA
VIAVFADMNNADYLEEIYSKAVELGYMPQEVKDRQIAALTAHFEKLPAQVNRETILIDGGLVSMQLMLTARAHGYDTNPI
GGYDKENIAETFGLDKERYVPVMLLSIGKAADEGYASYRLPIDTIAEWK
>Mature_209_residues
MIKTNDFMEIMKGRRSIRNYDPAVKISKEEMTEILEEATTAPSSVNAQPWRFLVIDSPEGKEKLAPLASFNQTQVTTSSA
VIAVFADMNNADYLEEIYSKAVELGYMPQEVKDRQIAALTAHFEKLPAQVNRETILIDGGLVSMQLMLTARAHGYDTNPI
GGYDKENIAETFGLDKERYVPVMLLSIGKAADEGYASYRLPIDTIAEWK

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family

Homologues:

Organism=Escherichia coli, GI1786792, Length=216, Percent_Identity=25.462962962963, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YDGI_BACSU (P96707)

Other databases:

- EMBL:   AB001488
- EMBL:   AL009126
- PIR:   C69783
- RefSeq:   NP_388447.1
- ProteinModelPortal:   P96707
- SMR:   P96707
- EnsemblBacteria:   EBBACT00000003471
- GeneID:   938001
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU05660
- NMPDR:   fig|224308.1.peg.566
- GenoList:   BSU05660
- GeneTree:   EBGT00070000031913
- HOGENOM:   HBG751302
- OMA:   MKAILED
- PhylomeDB:   P96707
- ProtClustDB:   CLSK886786
- BioCyc:   BSUB:BSU05660-MONOMER
- InterPro:   IPR000415

Pfam domain/function: PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23360; Mature: 23360

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTNDFMEIMKGRRSIRNYDPAVKISKEEMTEILEEATTAPSSVNAQPWRFLVIDSPEG
CCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
KEKLAPLASFNQTQVTTSSAVIAVFADMNNADYLEEIYSKAVELGYMPQEVKDRQIAALT
HHHHHHHHCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHH
AHFEKLPAQVNRETILIDGGLVSMQLMLTARAHGYDTNPIGGYDKENIAETFGLDKERYV
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHCH
PVMLLSIGKAADEGYASYRLPIDTIAEWK
HHHEEECCCCCCCCCCEEECCHHHHCCCC
>Mature Secondary Structure
MIKTNDFMEIMKGRRSIRNYDPAVKISKEEMTEILEEATTAPSSVNAQPWRFLVIDSPEG
CCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
KEKLAPLASFNQTQVTTSSAVIAVFADMNNADYLEEIYSKAVELGYMPQEVKDRQIAALT
HHHHHHHHCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHH
AHFEKLPAQVNRETILIDGGLVSMQLMLTARAHGYDTNPIGGYDKENIAETFGLDKERYV
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHCH
PVMLLSIGKAADEGYASYRLPIDTIAEWK
HHHEEECCCCCCCCCCEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377