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The map label for this gene is yaaI

Identifier: 16077085

GI number: 16077085

Start: 25221

End: 25766

Strand: Reverse

Name: yaaI

Synonym: BSU00170

Alternate gene names: 16077085

Gene position: 25766-25221 (Counterclockwise)

Preceding gene: 16077105

Following gene: 16077084

Centisome position: 0.61

GC content: 37.73

Gene sequence:

>546_bases
TTGTCCAAAGCAGATAAAGCCCTTCTCATTGTAGACATGATCAATAATTTTGAATTCGATATGGGGGAAACCCTCGCTAA
AAAAACAGAAAAAATCGTTCCTCATATTTTATCATTAAAGGAGCACGCGAGACAAAATGAATGGCCGATTATTTACATCA
ATGATCATTATGGACTTTGGCAAGCTGACATTAAAAACATACAGCAAGAATGCACAAATGAAAGAAGCAAGGATATCATC
ACAAAGATAGCTCCGGTAGATGCTGATTATTTTTTAATCAAACCGAAACATTCCGCTTTCTACGAAACAGCACTTCACAC
CCTTCTTACTGAGTTGCAGGTGAGACATATTATCATAACAGGTATAGCCGGCAATATATGCGTGCTGTTTACCGCTAATG
ACGCTTATATGAGGGAATACAGCATCACGATTCCTAAGGACTGTATCGCGTCAAACAGTGACGAAGATAACGAATTCGCC
TTAACCATGATGGAGAATGTTCTTTTCGCAGAAATTACGACGGAAGAACAAATTATAGAAAAATAA

Upstream 100 bases:

>100_bases
TTCATCTTGTGTCATAAGGATAAGCTCTCTTTTCAAAGTTTTTTCATTGCCTAAAAAGGCTACATATTAACTATAACTGA
AACGGAAAGGAGACTGTCGA

Downstream 100 bases:

>100_bases
ACATGATCAGCGCTTTTCTTTCATACATTGATAGCGATATGAAAGGAGGCGTTTTTCATTCAAATTTATGTGGTAAAACA
AGGCGACACTCTTTCTGCTA

Product: isochorismatase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSKADKALLIVDMINNFEFDMGETLAKKTEKIVPHILSLKEHARQNEWPIIYINDHYGLWQADIKNIQQECTNERSKDII
TKIAPVDADYFLIKPKHSAFYETALHTLLTELQVRHIIITGIAGNICVLFTANDAYMREYSITIPKDCIASNSDEDNEFA
LTMMENVLFAEITTEEQIIEK

Sequences:

>Translated_181_residues
MSKADKALLIVDMINNFEFDMGETLAKKTEKIVPHILSLKEHARQNEWPIIYINDHYGLWQADIKNIQQECTNERSKDII
TKIAPVDADYFLIKPKHSAFYETALHTLLTELQVRHIIITGIAGNICVLFTANDAYMREYSITIPKDCIASNSDEDNEFA
LTMMENVLFAEITTEEQIIEK
>Mature_180_residues
SKADKALLIVDMINNFEFDMGETLAKKTEKIVPHILSLKEHARQNEWPIIYINDHYGLWQADIKNIQQECTNERSKDIIT
KIAPVDADYFLIKPKHSAFYETALHTLLTELQVRHIIITGIAGNICVLFTANDAYMREYSITIPKDCIASNSDEDNEFAL
TMMENVLFAEITTEEQIIEK

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family

Homologues:

Organism=Escherichia coli, GI1786811, Length=184, Percent_Identity=25, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YAAI_BACSU (P37532)

Other databases:

- EMBL:   D26185
- EMBL:   AL009126
- PIR:   S66047
- RefSeq:   NP_387898.1
- ProteinModelPortal:   P37532
- SMR:   P37532
- EnsemblBacteria:   EBBACT00000002580
- GeneID:   937036
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU00170
- NMPDR:   fig|224308.1.peg.17
- GenoList:   BSU00170
- GeneTree:   EBGT00050000000750
- HOGENOM:   HBG752434
- OMA:   NDAYMRE
- PhylomeDB:   P37532
- ProtClustDB:   CLSK2484777
- BioCyc:   BSUB:BSU00170-MONOMER
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 20809; Mature: 20678

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKADKALLIVDMINNFEFDMGETLAKKTEKIVPHILSLKEHARQNEWPIIYINDHYGLW
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCH
QADIKNIQQECTNERSKDIITKIAPVDADYFLIKPKHSAFYETALHTLLTELQVRHIIIT
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHEEEEE
GIAGNICVLFTANDAYMREYSITIPKDCIASNSDEDNEFALTMMENVLFAEITTEEQIIE
ECCCCEEEEEECCCCEEEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHEEECCHHHHHC
K
C
>Mature Secondary Structure 
SKADKALLIVDMINNFEFDMGETLAKKTEKIVPHILSLKEHARQNEWPIIYINDHYGLW
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCH
QADIKNIQQECTNERSKDIITKIAPVDADYFLIKPKHSAFYETALHTLLTELQVRHIIIT
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHEEEEE
GIAGNICVLFTANDAYMREYSITIPKDCIASNSDEDNEFALTMMENVLFAEITTEEQIIE
ECCCCEEEEEECCCCEEEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHEEECCHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377