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The map label for this gene is nuoB2

Identifier: 159901492

GI number: 159901492

Start: 6317285

End: 6318037

Strand: Direct

Name: nuoB2

Synonym: Haur_4981

Alternate gene names: 159901492

Gene position: 6317285-6318037 (Clockwise)

Preceding gene: 159901491

Following gene: 159901493

Centisome position: 99.54

GC content: 53.78

Gene sequence:

>753_bases
ATGTCTGATCTACAAACGCAAATGGATCTTGAGCAGCAGGGGGTGCTGTTTACGACCATCAATAAGCTGTACAACTGGGG
TCGCCGCTCTTCGGTGTGGCCGATGCAGTTCGGGTTAGCTTGCTGCGCGATTGAGATGATTGCCGCAGCTGCTTCGCGTT
ATGATATTTCGCGCTTTGGCTCGGAACTCTTTCGCGCCTCGCCCCGCCAAGCTGACGTGATGATCGTTGCTGGGACTGTG
ACCAAGAAAATGGTTCCACAGGTTGTTCGTTTGTTTAACCAAATGCCTGAGCCACGCTATGTGATTTCGATGGGAGCTTG
TGCAACCTCTGGTGGCCCCTTCCGCGATGGTTACAATGTGGTGCGCGGCGTTGATTTATATGTTCCGGTTGATGTGTATA
TTCCGGGTTGCCCACCTCGCCCTGAAGCTTTGTTGCACGCCTTGATGACCTTGCAAGCGCAAATCGATGTGCAATCATTA
CAACAAGTGCGTTGGTATGGCGATGATGAAGGCGTGGTCAACGAGTTTCCAGTGCCAATTTTTGGTGCTGAAGGCTTGGA
AGTGCCTGGAATCCCTGGGACTGCCGACCCCGTGGGTGGCACGCCCAAAATGCCGCCATTTGTTAGCCCAGCCTTGGGTG
GCAAAGGCGAACGCGATGCATCAATTAAAGCCTTGGAAGGCAATCAGCCCAAGCCAATGTTGCGGGCTGGCACGGTAACG
ATCGAGGAGGTAGCCCATGGCATTGCTGGCTAG

Upstream 100 bases:

>100_bases
GAGTGGATCTAAACGACGAATAGGGGAGTGACGGCGCGGAGGCGGCGCGGCTGCGCTGGGCCTTGGCCTAATTCAATTCA
CCATTTTGCCCGGAGGGCGT

Downstream 100 bases:

>100_bases
AGGCGAACTGCGCTCGATCCTGCAAACCGAAATGCCCAACGCCCTAGATACCCGTTGGGATACCCAAGCAACCGACCAAC
CCGAAGCTCATGGCGAAGGC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSDLQTQMDLEQQGVLFTTINKLYNWGRRSSVWPMQFGLACCAIEMIAAAASRYDISRFGSELFRASPRQADVMIVAGTV
TKKMVPQVVRLFNQMPEPRYVISMGACATSGGPFRDGYNVVRGVDLYVPVDVYIPGCPPRPEALLHALMTLQAQIDVQSL
QQVRWYGDDEGVVNEFPVPIFGAEGLEVPGIPGTADPVGGTPKMPPFVSPALGGKGERDASIKALEGNQPKPMLRAGTVT
IEEVAHGIAG

Sequences:

>Translated_250_residues
MSDLQTQMDLEQQGVLFTTINKLYNWGRRSSVWPMQFGLACCAIEMIAAAASRYDISRFGSELFRASPRQADVMIVAGTV
TKKMVPQVVRLFNQMPEPRYVISMGACATSGGPFRDGYNVVRGVDLYVPVDVYIPGCPPRPEALLHALMTLQAQIDVQSL
QQVRWYGDDEGVVNEFPVPIFGAEGLEVPGIPGTADPVGGTPKMPPFVSPALGGKGERDASIKALEGNQPKPMLRAGTVT
IEEVAHGIAG
>Mature_249_residues
SDLQTQMDLEQQGVLFTTINKLYNWGRRSSVWPMQFGLACCAIEMIAAAASRYDISRFGSELFRASPRQADVMIVAGTVT
KKMVPQVVRLFNQMPEPRYVISMGACATSGGPFRDGYNVVRGVDLYVPVDVYIPGCPPRPEALLHALMTLQAQIDVQSLQ
QVRWYGDDEGVVNEFPVPIFGAEGLEVPGIPGTADPVGGTPKMPPFVSPALGGKGERDASIKALEGNQPKPMLRAGTVTI
EEVAHGIAG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=151, Percent_Identity=58.2781456953642, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1788624, Length=166, Percent_Identity=53.6144578313253, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1789074, Length=125, Percent_Identity=43.2, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=41.8032786885246, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=52.3809523809524, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI18859983, Length=151, Percent_Identity=54.3046357615894, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24642371, Length=151, Percent_Identity=54.3046357615894, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24651058, Length=147, Percent_Identity=55.7823129251701, Blast_Score=181, Evalue=4e-46,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB2_HERA2 (A9B478)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547739.1
- ProteinModelPortal:   A9B478
- SMR:   A9B478
- GeneID:   5736817
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4981
- HOGENOM:   HBG553221
- OMA:   NQMPEPR
- ProtClustDB:   PRK14819
- BioCyc:   HAUR316274:HAUR_4981-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 27009; Mature: 26878

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLQTQMDLEQQGVLFTTINKLYNWGRRSSVWPMQFGLACCAIEMIAAAASRYDISRFG
CCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SELFRASPRQADVMIVAGTVTKKMVPQVVRLFNQMPEPRYVISMGACATSGGPFRDGYNV
HHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHH
VRGVDLYVPVDVYIPGCPPRPEALLHALMTLQAQIDVQSLQQVRWYGDDEGVVNEFPVPI
HHCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCE
FGAEGLEVPGIPGTADPVGGTPKMPPFVSPALGGKGERDASIKALEGNQPKPMLRAGTVT
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCEE
IEEVAHGIAG
HHHHHHHCCC
>Mature Secondary Structure 
SDLQTQMDLEQQGVLFTTINKLYNWGRRSSVWPMQFGLACCAIEMIAAAASRYDISRFG
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SELFRASPRQADVMIVAGTVTKKMVPQVVRLFNQMPEPRYVISMGACATSGGPFRDGYNV
HHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHH
VRGVDLYVPVDVYIPGCPPRPEALLHALMTLQAQIDVQSLQQVRWYGDDEGVVNEFPVPI
HHCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCE
FGAEGLEVPGIPGTADPVGGTPKMPPFVSPALGGKGERDASIKALEGNQPKPMLRAGTVT
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCEE
IEEVAHGIAG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA