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The map label for this gene is proS [H]

Identifier: 159901383

GI number: 159901383

Start: 6206479

End: 6208191

Strand: Direct

Name: proS [H]

Synonym: Haur_4872

Alternate gene names: 159901383

Gene position: 6206479-6208191 (Clockwise)

Preceding gene: 159901382

Following gene: 159901385

Centisome position: 97.79

GC content: 49.91

Gene sequence:

>1713_bases
ATGCGAATGTCGAAACTTGCAACGAGTACCACCAAAGAAGCACCAAATGATGCCGAATTAATTAGTCACTCGCTGTTGGT
GCGGGCTGGCTATGTCAAGCAACAATCAGCCGGAATCTACTCGTTGCTGCCGTTATGTGTACGTTCGACCCAGAAAATCT
CGCAAATTATTCGCGAAGAGATGGATGCGATTGACGGCCAAGAGTTGTTGATGCCAGTGGTCACCAGCGCAGATCTTTGG
CGCGAAACTGGTCGCTACGAAGGCGTGACCGAAGAATTGCTGCGCTTCAAAGATCGCAACAATAAAGATATGGTGTTGAA
CATGACCCATGAAGAAGTGGTCACCGACATCATGCGCCGCTACATCAAATCGTATCGCCAAATGCCCGTCATGGTCTATC
AGATTCAGACCAAATATCGTGACGAGGCCCGACCCCGTGGCGGGATCATTCGTTTGCGCGAGTTTACGATGAAGGATGCC
TATTCCTTCCATGCCACAATTGAAGATCTCGACCAATATTATGAGCAAGTGCTCAATGCCTACAAGCGGATTTACCAACG
CTGTGGCTTGCCAGTCGAAGTTGTGCAATCTGACCCGGGCATGATGGGTGGCAAGATTGCCCACGAATTTATGGCGGTCA
CACCAGCTGGCGAAGATACCCTGATCTTGTGTCCAAGCTGTGGTTATTCGGCCAATCGCGAAGTTGCCGTTGGAGCCAAC
CCAGCCTCAACCGCTGCGCTCGAAGCCCTAAGCCAAGGTGAAGCCAACACCAAAGATTTGGCTGGGCATTTCGGGGTTGC
ACCAACTCGCGTTATCAATATTAAACTCTATAAAACTGCCGAAGGGGTGTTGGTGGTAACCTATGCCCCAGCAGGCCGCG
AAATCAACCACATCAAATTGCAAAATGCCGTTGGCGCTGAATTGCAAAACGTCAGCGAAGCCGAATTGGCCAACTATGGC
TTGAGCGGTTTTGCTAGTGTTGCTCCACGCAGCCTCAAGGCTAGCGAAAACGTGCGCATTTTGGTCGATAGCGCTTTGAC
GACCGAACGCAATTTGGTTGGCGCAGCTGGCAAAGCTGGCCACTACGATCTCAATTTAAATTGGGAGCGGGATTTCACAG
GCGAAGTTGTCGATTTGACCGATGTGCTCGAAGGCCAACCTTGTAGCCGCTGTGGTGCGCCATTGACGATTGCTCGTGCG
ATCGAAGTTGGTAATATCTTCAAATTGGGCACCAAATATTCAGCCGCCATGAAATGTGTATTTGCCAACGAAAAGGGCGA
AATGCAACCAGCAATCATGGGTTGCTATGGCATTGGGGTGCAACGGATTTTGGCATCGTTGATTGAAAAGTTCAACGATG
CGCGTGGCATGATGCTGCCAATTACGGTTGCGCCATATCAAGTCCATGTGGTTGGCACCGATTATCAAAGCAATGCCGAA
ACTAAGAGCGTCGCTGATCAACTGTATAGCGATTTACAAGCCCAAGGCGTAACAGTATTATTGGATGATCGTGATGTTAA
TGCTGGCAATAAATTTGGCGATGCTGATTTGATTGGCAACCCAATTCAGGTGATTATCGGCTCGCGTGGCCTGAAAAATG
GTCAAGCTGAGGTCAAAATTCGCAGCACAGGCGAAACCACCATGGTTGCTTTGGCCGATCTGCTGAGCCATGTTTTGGGC
GTGCGCCAAGCAATGTTTGCGAGCTTGAGCTAA

Upstream 100 bases:

>100_bases
TAAGTGGTACAATTGACCTTCGCTACCGCCCAAGCGTGGAGCACCAATCAGATCAATCTGGCAATTGTTGCCAGCAGCAC
CAAATGGAGAACTAATTCGT

Downstream 100 bases:

>100_bases
TCTTTGAGGGGTCAGGGGCTAGAATGGCTAGTTTTGAGCTGGCTCTTGACCTCTGACCCTTGACCCCGACTTTTGGGCCA
CCGCTGAAGCAGGCCCATTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRMSKLATSTTKEAPNDAELISHSLLVRAGYVKQQSAGIYSLLPLCVRSTQKISQIIREEMDAIDGQELLMPVVTSADLW
RETGRYEGVTEELLRFKDRNNKDMVLNMTHEEVVTDIMRRYIKSYRQMPVMVYQIQTKYRDEARPRGGIIRLREFTMKDA
YSFHATIEDLDQYYEQVLNAYKRIYQRCGLPVEVVQSDPGMMGGKIAHEFMAVTPAGEDTLILCPSCGYSANREVAVGAN
PASTAALEALSQGEANTKDLAGHFGVAPTRVINIKLYKTAEGVLVVTYAPAGREINHIKLQNAVGAELQNVSEAELANYG
LSGFASVAPRSLKASENVRILVDSALTTERNLVGAAGKAGHYDLNLNWERDFTGEVVDLTDVLEGQPCSRCGAPLTIARA
IEVGNIFKLGTKYSAAMKCVFANEKGEMQPAIMGCYGIGVQRILASLIEKFNDARGMMLPITVAPYQVHVVGTDYQSNAE
TKSVADQLYSDLQAQGVTVLLDDRDVNAGNKFGDADLIGNPIQVIIGSRGLKNGQAEVKIRSTGETTMVALADLLSHVLG
VRQAMFASLS

Sequences:

>Translated_570_residues
MRMSKLATSTTKEAPNDAELISHSLLVRAGYVKQQSAGIYSLLPLCVRSTQKISQIIREEMDAIDGQELLMPVVTSADLW
RETGRYEGVTEELLRFKDRNNKDMVLNMTHEEVVTDIMRRYIKSYRQMPVMVYQIQTKYRDEARPRGGIIRLREFTMKDA
YSFHATIEDLDQYYEQVLNAYKRIYQRCGLPVEVVQSDPGMMGGKIAHEFMAVTPAGEDTLILCPSCGYSANREVAVGAN
PASTAALEALSQGEANTKDLAGHFGVAPTRVINIKLYKTAEGVLVVTYAPAGREINHIKLQNAVGAELQNVSEAELANYG
LSGFASVAPRSLKASENVRILVDSALTTERNLVGAAGKAGHYDLNLNWERDFTGEVVDLTDVLEGQPCSRCGAPLTIARA
IEVGNIFKLGTKYSAAMKCVFANEKGEMQPAIMGCYGIGVQRILASLIEKFNDARGMMLPITVAPYQVHVVGTDYQSNAE
TKSVADQLYSDLQAQGVTVLLDDRDVNAGNKFGDADLIGNPIQVIIGSRGLKNGQAEVKIRSTGETTMVALADLLSHVLG
VRQAMFASLS
>Mature_570_residues
MRMSKLATSTTKEAPNDAELISHSLLVRAGYVKQQSAGIYSLLPLCVRSTQKISQIIREEMDAIDGQELLMPVVTSADLW
RETGRYEGVTEELLRFKDRNNKDMVLNMTHEEVVTDIMRRYIKSYRQMPVMVYQIQTKYRDEARPRGGIIRLREFTMKDA
YSFHATIEDLDQYYEQVLNAYKRIYQRCGLPVEVVQSDPGMMGGKIAHEFMAVTPAGEDTLILCPSCGYSANREVAVGAN
PASTAALEALSQGEANTKDLAGHFGVAPTRVINIKLYKTAEGVLVVTYAPAGREINHIKLQNAVGAELQNVSEAELANYG
LSGFASVAPRSLKASENVRILVDSALTTERNLVGAAGKAGHYDLNLNWERDFTGEVVDLTDVLEGQPCSRCGAPLTIARA
IEVGNIFKLGTKYSAAMKCVFANEKGEMQPAIMGCYGIGVQRILASLIEKFNDARGMMLPITVAPYQVHVVGTDYQSNAE
TKSVADQLYSDLQAQGVTVLLDDRDVNAGNKFGDADLIGNPIQVIIGSRGLKNGQAEVKIRSTGETTMVALADLLSHVLG
VRQAMFASLS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=41.1504424778761, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=38.8791593695271, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI115532348, Length=226, Percent_Identity=36.283185840708, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI193203271, Length=92, Percent_Identity=33.695652173913, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=537, Percent_Identity=29.608938547486, Blast_Score=237, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24656200, Length=244, Percent_Identity=36.4754098360656, Blast_Score=177, Evalue=3e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 62366; Mature: 62366

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMSKLATSTTKEAPNDAELISHSLLVRAGYVKQQSAGIYSLLPLCVRSTQKISQIIREE
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHH
MDAIDGQELLMPVVTSADLWRETGRYEGVTEELLRFKDRNNKDMVLNMTHEEVVTDIMRR
HHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
YIKSYRQMPVMVYQIQTKYRDEARPRGGIIRLREFTMKDAYSFHATIEDLDQYYEQVLNA
HHHHHHCCCEEEEEEEHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKRIYQRCGLPVEVVQSDPGMMGGKIAHEFMAVTPAGEDTLILCPSCGYSANREVAVGAN
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEECCCCCCCEEECCCCCCCCCCEEEECCC
PASTAALEALSQGEANTKDLAGHFGVAPTRVINIKLYKTAEGVLVVTYAPAGREINHIKL
CHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEEEEEEECCCEEEEEECCCCCCCCEEEE
QNAVGAELQNVSEAELANYGLSGFASVAPRSLKASENVRILVDSALTTERNLVGAAGKAG
CCHHHHHHHCCCHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCC
HYDLNLNWERDFTGEVVDLTDVLEGQPCSRCGAPLTIARAIEVGNIFKLGTKYSAAMKCV
EEEEEEEECCCCCCCEEEHHHHHCCCCHHHCCCCEEEEHHHHHCCEEECCCCHHHEEEEE
FANEKGEMQPAIMGCYGIGVQRILASLIEKFNDARGMMLPITVAPYQVHVVGTDYQSNAE
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCCCCCCH
TKSVADQLYSDLQAQGVTVLLDDRDVNAGNKFGDADLIGNPIQVIIGSRGLKNGQAEVKI
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEECCCCCCCCCEEEEE
RSTGETTMVALADLLSHVLGVRQAMFASLS
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRMSKLATSTTKEAPNDAELISHSLLVRAGYVKQQSAGIYSLLPLCVRSTQKISQIIREE
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHH
MDAIDGQELLMPVVTSADLWRETGRYEGVTEELLRFKDRNNKDMVLNMTHEEVVTDIMRR
HHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
YIKSYRQMPVMVYQIQTKYRDEARPRGGIIRLREFTMKDAYSFHATIEDLDQYYEQVLNA
HHHHHHCCCEEEEEEEHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKRIYQRCGLPVEVVQSDPGMMGGKIAHEFMAVTPAGEDTLILCPSCGYSANREVAVGAN
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEECCCCCCCEEECCCCCCCCCCEEEECCC
PASTAALEALSQGEANTKDLAGHFGVAPTRVINIKLYKTAEGVLVVTYAPAGREINHIKL
CHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEEEEEEECCCEEEEEECCCCCCCCEEEE
QNAVGAELQNVSEAELANYGLSGFASVAPRSLKASENVRILVDSALTTERNLVGAAGKAG
CCHHHHHHHCCCHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCC
HYDLNLNWERDFTGEVVDLTDVLEGQPCSRCGAPLTIARAIEVGNIFKLGTKYSAAMKCV
EEEEEEEECCCCCCCEEEHHHHHCCCCHHHCCCCEEEEHHHHHCCEEECCCCHHHEEEEE
FANEKGEMQPAIMGCYGIGVQRILASLIEKFNDARGMMLPITVAPYQVHVVGTDYQSNAE
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCCCCCCH
TKSVADQLYSDLQAQGVTVLLDDRDVNAGNKFGDADLIGNPIQVIIGSRGLKNGQAEVKI
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEECCCCCCCCCEEEEE
RSTGETTMVALADLLSHVLGVRQAMFASLS
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]