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The map label for this gene is pleD [H]

Identifier: 159901244

GI number: 159901244

Start: 6043286

End: 6043657

Strand: Reverse

Name: pleD [H]

Synonym: Haur_4732

Alternate gene names: 159901244

Gene position: 6043657-6043286 (Counterclockwise)

Preceding gene: 159901245

Following gene: 159901235

Centisome position: 95.23

GC content: 47.04

Gene sequence:

>372_bases
ATGGCTTGTTTATTTATAGTTGATGATCAACCAGACACTCACATTATTCTCGCACGTTTACTTGAGGTTGATGGGTATAC
GGTGGTTGGGGCGCACAATGGTAGTGAAGCCCTGAAGCAGATACCTGTAGTTCGCCCAGCCTTGGTGTTGTTGGATCTAG
CCTTGCCGGGCATTGATGGTTTAACGGTTGCCAAACAGTTACGCATCAATACCAGCACCAAGCATGTGCCAATTATTGCC
ATGACGGCCTTTGGTGGCAACGATTATCGTACCCAAGCTATGGCAGCTGGTTGTAGCGAATATCTCTTAAAACCATTGGA
TTTTGCTAAACTTCGCGGGGTGATTGCGCAATTGGTGCTACAAGTGGCCTAA

Upstream 100 bases:

>100_bases
TAAAGAACTACCCAATCAGGGACTCAATTCCTATTGTCCAATGAGTGTTTTGTCGAAGCATCGATCGAGATTTCATACCT
CTACGGACACTGGAGCAGGT

Downstream 100 bases:

>100_bases
ATGCGTGACGACAAGTGGGGTGTCGGCATGAAACCCGAGTAATAATCGGGCTTTTTTATTGCCAAAACATGGCAGCATGG
AGTTGATCCATGCTGCCATG

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MACLFIVDDQPDTHIILARLLEVDGYTVVGAHNGSEALKQIPVVRPALVLLDLALPGIDGLTVAKQLRINTSTKHVPIIA
MTAFGGNDYRTQAMAAGCSEYLLKPLDFAKLRGVIAQLVLQVA

Sequences:

>Translated_123_residues
MACLFIVDDQPDTHIILARLLEVDGYTVVGAHNGSEALKQIPVVRPALVLLDLALPGIDGLTVAKQLRINTSTKHVPIIA
MTAFGGNDYRTQAMAAGCSEYLLKPLDFAKLRGVIAQLVLQVA
>Mature_122_residues
ACLFIVDDQPDTHIILARLLEVDGYTVVGAHNGSEALKQIPVVRPALVLLDLALPGIDGLTVAKQLRINTSTKHVPIIAM
TAFGGNDYRTQAMAAGCSEYLLKPLDFAKLRGVIAQLVLQVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=119, Percent_Identity=30.2521008403361, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI87082012, Length=111, Percent_Identity=32.4324324324324, Blast_Score=62, Evalue=9e-12,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=32.1739130434783, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=106, Percent_Identity=33.9622641509434, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI1786784, Length=108, Percent_Identity=32.4074074074074, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 13178; Mature: 13047

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACLFIVDDQPDTHIILARLLEVDGYTVVGAHNGSEALKQIPVVRPALVLLDLALPGIDG
CEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCCCC
LTVAKQLRINTSTKHVPIIAMTAFGGNDYRTQAMAAGCSEYLLKPLDFAKLRGVIAQLVL
HHHHHEEEECCCCCCEEEEEEEEECCCCHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHH
QVA
HCC
>Mature Secondary Structure 
ACLFIVDDQPDTHIILARLLEVDGYTVVGAHNGSEALKQIPVVRPALVLLDLALPGIDG
EEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCCCC
LTVAKQLRINTSTKHVPIIAMTAFGGNDYRTQAMAAGCSEYLLKPLDFAKLRGVIAQLVL
HHHHHEEEECCCCCCEEEEEEEEECCCCHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHH
QVA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA