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The map label for this gene is fruB(HI) [H]

Identifier: 159901224

GI number: 159901224

Start: 6018411

End: 6020888

Strand: Direct

Name: fruB(HI) [H]

Synonym: Haur_4712

Alternate gene names: 159901224

Gene position: 6018411-6020888 (Clockwise)

Preceding gene: 159901220

Following gene: 159901225

Centisome position: 94.83

GC content: 54.64

Gene sequence:

>2478_bases
GTGATCGAGCTACAGCAGCGTCATATCCAACTCAAGAGTCAGCCCGCCAACAAGCAAGCGGCAATTCAACAAGCAGGCCA
GCTTCTGGTTGCCAATGGGCAGATCGAGTCTGGCTATATTCAAAGTATGCTCCAACGCGAAGCCCTTTCGAATACTTATC
TTGGCAATGGCATCGCAATTCCTCATGGTTTGCCCGAAGCCCGCGATCTGATTCGCCAAACCGGAATTGTGGTACTGCAA
GTGCCCAACGGGGTGCAGTGGAATCCAGGTGAAACTGTGCACTTAATCGTTGGCATCGCCGCTCGCTCCGATGAACATAT
TGCAATTTTGCGCCAACTTACCCGCGTGCTTGGCGATAAAACCTTAGTTGACCAACTCACCCACACCACCAATCCTGATG
ATCTGATTCGCGCATTGACTGGCGCTGAAACGGCTCCCGCAGCCTCAGCGGCTCCAGCTATCAGTGTGGCGGTTGCCAGT
GACCAGCCCTTTTTTGAAACCAAAGTTTTGAATCCCACTGGCTTTCATGCTCGCCCGGCCACAACCTTTGTTGATTGCGC
TAAGCGTTTTCAAGCGGAAGTGCGCATACGTTATGGCTCGCGTGAGGCCAATGGCAAAAGCTTAATCTCTATTTTGCAAC
TTGGCATTCCCCATGGCGCGACGATTCAGGTCACGGCTCAAGGTGCTGATGCCGCTGCGGCGCTACGTGGTTTACAACAG
GCATTGGCCCAAGGCTTAGCTGATGAAACAGCGGAAACGCTTCCAACACGCGCTGCAGTCGATGTGCGCTGGACACCCAA
GGCCGTTGAGCAGACGATCAATGGCATTAGCGCCGCGCCAGGCTTGGCGATTGGCTTGCTCCGCCGTTATAGCCATAGCG
AGTTGGTGATCGAAGATCGGCCAAGTGACCCGATGGTTGAGGTCAATCGCTTTGAGCTGGCATTGGCCGCTGCCCAAGCC
GAGCTGTCAACGCTCTATGATGAAGTGCAAGCGCGAATTGGCTCAGGCAAGGCGGCGATTTTCCGCGTGCATAGCGAAAT
GTTGAGCGATACCAGCCTTGTGCAACAAACCGTCTCGTTGTTATTTGAAAAACATAGCGCTGAATGGGCTTGGCATCAGG
TGATCAGCGGGCGCGTGGCCCAAATCGAAAAACTTGACGATCAAGTGCTAGCTGGCCGTGCGGTTGATCTGAGTGATGTT
GGTCAGCGGGTGTTACGCCAGTTGATCGGCGGCGATCATCAACGGCCTTTGAATGCAGCCACGCCAGTGATTATTTTGGC
CGACGATTTGACTCCCTCAGATACCGCCGCCTTTGACCCCGACACTATTTTGGGCTTTGGCACAGTACGCGGCGGCCCAA
CTTCGCACACCGCAATTTTAGCGCGTTCGCTGGGAATTCCAGCAATCGTTGGGGCTGGCGAAGGCTTGTTGAATTTGCCC
GAAGAAGCAATTGCAATTCTCGATGGCTACAACGGCAAATTGTATCTCAACCCAAGTAGTGCCGATATCGAGGCCGCGAC
CAACTTGCAAGCCGAATTGGCCGAACAACACGAGCGTGCTCAAGCTACGCGCTTCGAGCCAGCCCAAACCAGCGATGGCC
ATCGAATCGAAATTGCTGCCAACATCAATCGCGTTGCTGATGCGAGTGTTGCCGCCGCTGCGGGAGCCGAAGGCGTGGGC
TTGATGCGCACCGAATTTCTGTTCCTTGAGCGCGATAGTGCTCCCAGCGAGGAAGAACAATTCGAGGCCTATCGCGATAT
GGTGCAAGCGATGGCGGGCCACTCGGTGATTATTCGCACGCTGGATATTGGCGGCGATAAAGTTGTGCCCTATCTCGATT
TGCCCAAAGAAGATAATTCGTTCTTGGGCATTCGCGGGATTCGACTCTGCCTAGCCCGACCTGAATTATTTATCCCACAG
CTCCGTGCGATTTATCGCGCCGCCGCTTTTGGGCCGCTCAAAATTATGTTCCCAATGATTGCCACCTTGGAAGATTGGTA
CGCCGCCCGCGATTTGGCTGAGCAAGTGCGCCGCGAACTCGACGCGCCTCAAGTGCCACTCGGCATTATGGTCGAAGTGC
CATCGGCGGCAGTGCTCGCCGAACAATTTGCCCAAGAAGTCGATTTCTTCTCGATTGGCACCAACGATTTGACCCAATAC
ACCCTAGCAATGGATCGTTTACACCCACAATTAGCTAGCCAAGCCGATGGCCTGCATCCAGCGGTGCTGCGAATGATCGA
CCTGACCGCCCACGCCGCCAACGCCCACAACAAATGGGTTGGTGTATGTGGTGGCATTGCCGCCGATGCCCGTGGTTCCT
TGATTTTGGTGGGCTTGGGCGTGCATGAACTCAGCGTCAGCGTACCAGCCATCGCCGAACTCAAAGCTGCCATTCGTCAA
CATAGCTTGGCCGATTTACAAGCACTCGCGCAACGTGCCTTGGCATGTCGCAGTGCTGCCGAGGTACATCAATTATGA

Upstream 100 bases:

>100_bases
CGATTACAGTAATTTCAGCAAACAAATTCAAAGCTCTCCCCGCAACCCTACCTGAGCCACTACTCACAACGGGTAGCCAT
CAGCGACAAAGGAGTCCGAC

Downstream 100 bases:

>100_bases
GTATCGTTACATTAACGCTCAATCCCGCCATTGACCAAACGATTTTCATCAATAATTTTCAGCTGGAAACCGTCAATCGG
GCCTACGATCTCAAGAGCCA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 825; Mature: 825

Protein sequence:

>825_residues
MIELQQRHIQLKSQPANKQAAIQQAGQLLVANGQIESGYIQSMLQREALSNTYLGNGIAIPHGLPEARDLIRQTGIVVLQ
VPNGVQWNPGETVHLIVGIAARSDEHIAILRQLTRVLGDKTLVDQLTHTTNPDDLIRALTGAETAPAASAAPAISVAVAS
DQPFFETKVLNPTGFHARPATTFVDCAKRFQAEVRIRYGSREANGKSLISILQLGIPHGATIQVTAQGADAAAALRGLQQ
ALAQGLADETAETLPTRAAVDVRWTPKAVEQTINGISAAPGLAIGLLRRYSHSELVIEDRPSDPMVEVNRFELALAAAQA
ELSTLYDEVQARIGSGKAAIFRVHSEMLSDTSLVQQTVSLLFEKHSAEWAWHQVISGRVAQIEKLDDQVLAGRAVDLSDV
GQRVLRQLIGGDHQRPLNAATPVIILADDLTPSDTAAFDPDTILGFGTVRGGPTSHTAILARSLGIPAIVGAGEGLLNLP
EEAIAILDGYNGKLYLNPSSADIEAATNLQAELAEQHERAQATRFEPAQTSDGHRIEIAANINRVADASVAAAAGAEGVG
LMRTEFLFLERDSAPSEEEQFEAYRDMVQAMAGHSVIIRTLDIGGDKVVPYLDLPKEDNSFLGIRGIRLCLARPELFIPQ
LRAIYRAAAFGPLKIMFPMIATLEDWYAARDLAEQVRRELDAPQVPLGIMVEVPSAAVLAEQFAQEVDFFSIGTNDLTQY
TLAMDRLHPQLASQADGLHPAVLRMIDLTAHAANAHNKWVGVCGGIAADARGSLILVGLGVHELSVSVPAIAELKAAIRQ
HSLADLQALAQRALACRSAAEVHQL

Sequences:

>Translated_825_residues
MIELQQRHIQLKSQPANKQAAIQQAGQLLVANGQIESGYIQSMLQREALSNTYLGNGIAIPHGLPEARDLIRQTGIVVLQ
VPNGVQWNPGETVHLIVGIAARSDEHIAILRQLTRVLGDKTLVDQLTHTTNPDDLIRALTGAETAPAASAAPAISVAVAS
DQPFFETKVLNPTGFHARPATTFVDCAKRFQAEVRIRYGSREANGKSLISILQLGIPHGATIQVTAQGADAAAALRGLQQ
ALAQGLADETAETLPTRAAVDVRWTPKAVEQTINGISAAPGLAIGLLRRYSHSELVIEDRPSDPMVEVNRFELALAAAQA
ELSTLYDEVQARIGSGKAAIFRVHSEMLSDTSLVQQTVSLLFEKHSAEWAWHQVISGRVAQIEKLDDQVLAGRAVDLSDV
GQRVLRQLIGGDHQRPLNAATPVIILADDLTPSDTAAFDPDTILGFGTVRGGPTSHTAILARSLGIPAIVGAGEGLLNLP
EEAIAILDGYNGKLYLNPSSADIEAATNLQAELAEQHERAQATRFEPAQTSDGHRIEIAANINRVADASVAAAAGAEGVG
LMRTEFLFLERDSAPSEEEQFEAYRDMVQAMAGHSVIIRTLDIGGDKVVPYLDLPKEDNSFLGIRGIRLCLARPELFIPQ
LRAIYRAAAFGPLKIMFPMIATLEDWYAARDLAEQVRRELDAPQVPLGIMVEVPSAAVLAEQFAQEVDFFSIGTNDLTQY
TLAMDRLHPQLASQADGLHPAVLRMIDLTAHAANAHNKWVGVCGGIAADARGSLILVGLGVHELSVSVPAIAELKAAIRQ
HSLADLQALAQRALACRSAAEVHQL
>Mature_825_residues
MIELQQRHIQLKSQPANKQAAIQQAGQLLVANGQIESGYIQSMLQREALSNTYLGNGIAIPHGLPEARDLIRQTGIVVLQ
VPNGVQWNPGETVHLIVGIAARSDEHIAILRQLTRVLGDKTLVDQLTHTTNPDDLIRALTGAETAPAASAAPAISVAVAS
DQPFFETKVLNPTGFHARPATTFVDCAKRFQAEVRIRYGSREANGKSLISILQLGIPHGATIQVTAQGADAAAALRGLQQ
ALAQGLADETAETLPTRAAVDVRWTPKAVEQTINGISAAPGLAIGLLRRYSHSELVIEDRPSDPMVEVNRFELALAAAQA
ELSTLYDEVQARIGSGKAAIFRVHSEMLSDTSLVQQTVSLLFEKHSAEWAWHQVISGRVAQIEKLDDQVLAGRAVDLSDV
GQRVLRQLIGGDHQRPLNAATPVIILADDLTPSDTAAFDPDTILGFGTVRGGPTSHTAILARSLGIPAIVGAGEGLLNLP
EEAIAILDGYNGKLYLNPSSADIEAATNLQAELAEQHERAQATRFEPAQTSDGHRIEIAANINRVADASVAAAAGAEGVG
LMRTEFLFLERDSAPSEEEQFEAYRDMVQAMAGHSVIIRTLDIGGDKVVPYLDLPKEDNSFLGIRGIRLCLARPELFIPQ
LRAIYRAAAFGPLKIMFPMIATLEDWYAARDLAEQVRRELDAPQVPLGIMVEVPSAAVLAEQFAQEVDFFSIGTNDLTQY
TLAMDRLHPQLASQADGLHPAVLRMIDLTAHAANAHNKWVGVCGGIAADARGSLILVGLGVHELSVSVPAIAELKAAIRQ
HSLADLQALAQRALACRSAAEVHQL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=563, Percent_Identity=42.9840142095915, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI48994992, Length=679, Percent_Identity=34.1678939617084, Blast_Score=326, Evalue=3e-90,
Organism=Escherichia coli, GI1788726, Length=680, Percent_Identity=33.8235294117647, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1789193, Length=566, Percent_Identity=36.2190812720848, Blast_Score=300, Evalue=3e-82,
Organism=Escherichia coli, GI1788494, Length=141, Percent_Identity=42.5531914893617, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1790027, Length=143, Percent_Identity=41.958041958042, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI226510935, Length=324, Percent_Identity=29.0123456790123, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1787994, Length=401, Percent_Identity=27.1820448877805, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88449; Mature: 88449

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELQQRHIQLKSQPANKQAAIQQAGQLLVANGQIESGYIQSMLQREALSNTYLGNGIAI
CCCCCCHHHECCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEC
PHGLPEARDLIRQTGIVVLQVPNGVQWNPGETVHLIVGIAARSDEHIAILRQLTRVLGDK
CCCCHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCH
TLVDQLTHTTNPDDLIRALTGAETAPAASAAPAISVAVASDQPFFETKVLNPTGFHARPA
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCC
TTFVDCAKRFQAEVRIRYGSREANGKSLISILQLGIPHGATIQVTAQGADAAAALRGLQQ
HHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
ALAQGLADETAETLPTRAAVDVRWTPKAVEQTINGISAAPGLAIGLLRRYSHSELVIEDR
HHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECC
PSDPMVEVNRFELALAAAQAELSTLYDEVQARIGSGKAAIFRVHSEMLSDTSLVQQTVSL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHH
LFEKHSAEWAWHQVISGRVAQIEKLDDQVLAGRAVDLSDVGQRVLRQLIGGDHQRPLNAA
HHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TPVIILADDLTPSDTAAFDPDTILGFGTVRGGPTSHTAILARSLGIPAIVGAGEGLLNLP
CCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
EEAIAILDGYNGKLYLNPSSADIEAATNLQAELAEQHERAQATRFEPAQTSDGHRIEIAA
HHHHHHEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
NINRVADASVAAAAGAEGVGLMRTEFLFLERDSAPSEEEQFEAYRDMVQAMAGHSVIIRT
CCCHHHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
LDIGGDKVVPYLDLPKEDNSFLGIRGIRLCLARPELFIPQLRAIYRAAAFGPLKIMFPMI
EECCCCCCCEEECCCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
ATLEDWYAARDLAEQVRRELDAPQVPLGIMVEVPSAAVLAEQFAQEVDFFSIGTNDLTQY
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHH
TLAMDRLHPQLASQADGLHPAVLRMIDLTAHAANAHNKWVGVCGGIAADARGSLILVGLG
HHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEC
VHELSVSVPAIAELKAAIRQHSLADLQALAQRALACRSAAEVHQL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIELQQRHIQLKSQPANKQAAIQQAGQLLVANGQIESGYIQSMLQREALSNTYLGNGIAI
CCCCCCHHHECCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEC
PHGLPEARDLIRQTGIVVLQVPNGVQWNPGETVHLIVGIAARSDEHIAILRQLTRVLGDK
CCCCHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCH
TLVDQLTHTTNPDDLIRALTGAETAPAASAAPAISVAVASDQPFFETKVLNPTGFHARPA
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCC
TTFVDCAKRFQAEVRIRYGSREANGKSLISILQLGIPHGATIQVTAQGADAAAALRGLQQ
HHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
ALAQGLADETAETLPTRAAVDVRWTPKAVEQTINGISAAPGLAIGLLRRYSHSELVIEDR
HHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECC
PSDPMVEVNRFELALAAAQAELSTLYDEVQARIGSGKAAIFRVHSEMLSDTSLVQQTVSL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHH
LFEKHSAEWAWHQVISGRVAQIEKLDDQVLAGRAVDLSDVGQRVLRQLIGGDHQRPLNAA
HHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TPVIILADDLTPSDTAAFDPDTILGFGTVRGGPTSHTAILARSLGIPAIVGAGEGLLNLP
CCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
EEAIAILDGYNGKLYLNPSSADIEAATNLQAELAEQHERAQATRFEPAQTSDGHRIEIAA
HHHHHHEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
NINRVADASVAAAAGAEGVGLMRTEFLFLERDSAPSEEEQFEAYRDMVQAMAGHSVIIRT
CCCHHHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
LDIGGDKVVPYLDLPKEDNSFLGIRGIRLCLARPELFIPQLRAIYRAAAFGPLKIMFPMI
EECCCCCCCEEECCCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
ATLEDWYAARDLAEQVRRELDAPQVPLGIMVEVPSAAVLAEQFAQEVDFFSIGTNDLTQY
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHH
TLAMDRLHPQLASQADGLHPAVLRMIDLTAHAANAHNKWVGVCGGIAADARGSLILVGLG
HHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEC
VHELSVSVPAIAELKAAIRQHSLADLQALAQRALACRSAAEVHQL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193161 [H]