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The map label for this gene is appA [H]

Identifier: 159901195

GI number: 159901195

Start: 5981095

End: 5982789

Strand: Reverse

Name: appA [H]

Synonym: Haur_4683

Alternate gene names: 159901195

Gene position: 5982789-5981095 (Counterclockwise)

Preceding gene: 159901196

Following gene: 159901194

Centisome position: 94.27

GC content: 52.92

Gene sequence:

>1695_bases
ATGGTTCGACGTATTCGTTGGCAAATTGGAGCCACGTTGGTTTGTGCTAGTCTGGTTTTGTTGTTGTTGGCGCATCTTGC
GCTAGCAACAACAGCAGGCGGCGAGGCAGGCGTTGGCGGGGTCTATCGCGAGGCCTTGGTTGGCGAGGTCAATGATCTTA
ACCCATTGCGCGGTAGTGCCCAAACCCGCGCCGAGGCCGACTTAAGTGCTTTGTTGTTTGAAGGCTTGACCCGCGTTGAG
GCAACTGGCGTGGTCGTGCCCGATCTCGCCGAAAATTGGCAGGTCAGCAGCAATAATCTGGTTTATACCATGACCTTGCG
CACTGATGTGCGCTGGCACGATGCCACACCTTTTTCTGCAAACGATGTGATCTGGACGATCGACTGGATCAAAAGCCCAC
AATTTAATGGCGACCCCAGCCTTAAGTTAGCTTGGCAAAATATCGCCGTCACCGCGTTGAATCCGCAAACTGTGCGTTTT
AACTTGCCAGTTGCTTTTGCGCCCTTCCTCAACCAACTAACTGTGCCAATTTTGCCCGCGCATCTGTTGCGCAACGCTAC
GCCCGACCAATGGCAAGCTTGGAATCAGCAACCAGTTGGCACTGGCAGTTTTCGCTTTGGTAAACGCGATGGCAACTTAA
TCGAGCTATTGGCCAACACTGATTACCGTCCGAATATTCCCAATAGTCGCCCCAATTTGGAGCGGCTGGAGTTTCGGCTT
TACGCAACCATGGAAGCTGCGCATGAAGCACTGCGCCGTGATTTGGTCGATGCGTTTGCCTACGATGTGACTGAACAAGG
CGAATTACCGTTGCCAATCGGCTATCGCCAGGTGCGTGCGCCTTTGGCTGATTATGTGGTCCTGACGATTAATTTGCGTC
GTCCGCCACTTGATGATTTACGGGTGCGCCAAGCGATCGATTATGCAGTTGACCCAACTACGTTGATTACTGAAGTGTTG
CAAAATCGGGCGTTGCCCTTGAATTCGCCAATTTTGCCTTCGTCGTGGGCGGTTGCTGAGGGTCTCACCGGCTATATTGA
TGATGCTGAGCAGAGTCGCGCCAATGGGTTGCTTGATCAAGCTGGTTGGCAGCGTGATGCCGAAGGGTGGCGGCGTAAAG
CTGGCCAATTGTTGCGCTTCGATTTGGTCAGTGCCAACAGCCCCGAACGCAGCGCCATCGCCGAACGCATTCAAGCCCAA
TTAGCCACGGTCGGTATTTCAAGCAGTTTGCAATTGGTTCCGAGCAGCAATTTGCAAGATACGCTGAGCCAACATACCTT
CGATTTAGCAATTCATGGTTGGTCGAATTTAGGCAGCGACCCCGATGTTTACGAGTTATGGCATTCGTCGCAGGTCAACG
CCGCCAACTTTGCTGGCCTCGAAGACCCAGCAATTGATGATTTATTGGTGCGTGCTCGCCAAACCACCAGCCAAGAAACC
CGCCGCGAATTGTATGCTGAATTTCAGGCCAAATGGCTAGCACTCGCGCCCTCAGTGATGCTCTATCAGCCGTTGTTGGA
GCAGCAGGTTGGACCATCGATCGAGGTGCTTGGTCTGGCTCCAATCAACCAACAAGCTGAAGTGCTCTATCGGCATAGCG
ATCGCTTCCGGCGGATCGCCAATTGGTATCTAGTATCCACCCGTCAAGTCTTGCCCAACTTCCGAAATGAACCACAAAAC
GAACGCCCACGCTAA

Upstream 100 bases:

>100_bases
CGCGGCGTTGAAAAAACCCTCTTTAATGTAACGATTGTGATCGCCAGCGTGTTAATTATTTTAGCACTCGTGCATCCCTT
CTTATTAACAGTATAAGCGT

Downstream 100 bases:

>100_bases
TCAGCGGGGCGTTGCGGCTTTTTAGGGTTGTTGATGAGAAAGGATCAGCCAAATGACTACCCAAACGCTCCCACACTGGG
ATCTTTCAGTTGTCTACCCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MVRRIRWQIGATLVCASLVLLLLAHLALATTAGGEAGVGGVYREALVGEVNDLNPLRGSAQTRAEADLSALLFEGLTRVE
ATGVVVPDLAENWQVSSNNLVYTMTLRTDVRWHDATPFSANDVIWTIDWIKSPQFNGDPSLKLAWQNIAVTALNPQTVRF
NLPVAFAPFLNQLTVPILPAHLLRNATPDQWQAWNQQPVGTGSFRFGKRDGNLIELLANTDYRPNIPNSRPNLERLEFRL
YATMEAAHEALRRDLVDAFAYDVTEQGELPLPIGYRQVRAPLADYVVLTINLRRPPLDDLRVRQAIDYAVDPTTLITEVL
QNRALPLNSPILPSSWAVAEGLTGYIDDAEQSRANGLLDQAGWQRDAEGWRRKAGQLLRFDLVSANSPERSAIAERIQAQ
LATVGISSSLQLVPSSNLQDTLSQHTFDLAIHGWSNLGSDPDVYELWHSSQVNAANFAGLEDPAIDDLLVRARQTTSQET
RRELYAEFQAKWLALAPSVMLYQPLLEQQVGPSIEVLGLAPINQQAEVLYRHSDRFRRIANWYLVSTRQVLPNFRNEPQN
ERPR

Sequences:

>Translated_564_residues
MVRRIRWQIGATLVCASLVLLLLAHLALATTAGGEAGVGGVYREALVGEVNDLNPLRGSAQTRAEADLSALLFEGLTRVE
ATGVVVPDLAENWQVSSNNLVYTMTLRTDVRWHDATPFSANDVIWTIDWIKSPQFNGDPSLKLAWQNIAVTALNPQTVRF
NLPVAFAPFLNQLTVPILPAHLLRNATPDQWQAWNQQPVGTGSFRFGKRDGNLIELLANTDYRPNIPNSRPNLERLEFRL
YATMEAAHEALRRDLVDAFAYDVTEQGELPLPIGYRQVRAPLADYVVLTINLRRPPLDDLRVRQAIDYAVDPTTLITEVL
QNRALPLNSPILPSSWAVAEGLTGYIDDAEQSRANGLLDQAGWQRDAEGWRRKAGQLLRFDLVSANSPERSAIAERIQAQ
LATVGISSSLQLVPSSNLQDTLSQHTFDLAIHGWSNLGSDPDVYELWHSSQVNAANFAGLEDPAIDDLLVRARQTTSQET
RRELYAEFQAKWLALAPSVMLYQPLLEQQVGPSIEVLGLAPINQQAEVLYRHSDRFRRIANWYLVSTRQVLPNFRNEPQN
ERPR
>Mature_564_residues
MVRRIRWQIGATLVCASLVLLLLAHLALATTAGGEAGVGGVYREALVGEVNDLNPLRGSAQTRAEADLSALLFEGLTRVE
ATGVVVPDLAENWQVSSNNLVYTMTLRTDVRWHDATPFSANDVIWTIDWIKSPQFNGDPSLKLAWQNIAVTALNPQTVRF
NLPVAFAPFLNQLTVPILPAHLLRNATPDQWQAWNQQPVGTGSFRFGKRDGNLIELLANTDYRPNIPNSRPNLERLEFRL
YATMEAAHEALRRDLVDAFAYDVTEQGELPLPIGYRQVRAPLADYVVLTINLRRPPLDDLRVRQAIDYAVDPTTLITEVL
QNRALPLNSPILPSSWAVAEGLTGYIDDAEQSRANGLLDQAGWQRDAEGWRRKAGQLLRFDLVSANSPERSAIAERIQAQ
LATVGISSSLQLVPSSNLQDTLSQHTFDLAIHGWSNLGSDPDVYELWHSSQVNAANFAGLEDPAIDDLLVRARQTTSQET
RRELYAEFQAKWLALAPSVMLYQPLLEQQVGPSIEVLGLAPINQQAEVLYRHSDRFRRIANWYLVSTRQVLPNFRNEPQN
ERPR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=546, Percent_Identity=23.992673992674, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789887, Length=509, Percent_Identity=25.7367387033399, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1787762, Length=461, Percent_Identity=24.9457700650759, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1787551, Length=510, Percent_Identity=25.4901960784314, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081878, Length=554, Percent_Identity=22.2021660649819, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787052, Length=451, Percent_Identity=22.6164079822616, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63002; Mature: 63002

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRIRWQIGATLVCASLVLLLLAHLALATTAGGEAGVGGVYREALVGEVNDLNPLRGSA
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
QTRAEADLSALLFEGLTRVEATGVVVPDLAENWQVSSNNLVYTMTLRTDVRWHDATPFSA
HHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEECCCCEEEEEEEEEECEECCCCCCCC
NDVIWTIDWIKSPQFNGDPSLKLAWQNIAVTALNPQTVRFNLPVAFAPFLNQLTVPILPA
CCEEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCEEEEECCCHHHHHHHCCCHHHHHH
HLLRNATPDQWQAWNQQPVGTGSFRFGKRDGNLIELLANTDYRPNIPNSRPNLERLEFRL
HHHCCCCCHHHHCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
YATMEAAHEALRRDLVDAFAYDVTEQGELPLPIGYRQVRAPLADYVVLTINLRRPPLDDL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHH
RVRQAIDYAVDPTTLITEVLQNRALPLNSPILPSSWAVAEGLTGYIDDAEQSRANGLLDQ
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHCCCHHH
AGWQRDAEGWRRKAGQLLRFDLVSANSPERSAIAERIQAQLATVGISSSLQLVPSSNLQD
HCCCCCHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHH
TLSQHTFDLAIHGWSNLGSDPDVYELWHSSQVNAANFAGLEDPAIDDLLVRARQTTSQET
HHHHCEEEEEEECCCCCCCCCCHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
RRELYAEFQAKWLALAPSVMLYQPLLEQQVGPSIEVLGLAPINQQAEVLYRHSDRFRRIA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
NWYLVSTRQVLPNFRNEPQNERPR
HHHEEEHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MVRRIRWQIGATLVCASLVLLLLAHLALATTAGGEAGVGGVYREALVGEVNDLNPLRGSA
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
QTRAEADLSALLFEGLTRVEATGVVVPDLAENWQVSSNNLVYTMTLRTDVRWHDATPFSA
HHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEECCCCEEEEEEEEEECEECCCCCCCC
NDVIWTIDWIKSPQFNGDPSLKLAWQNIAVTALNPQTVRFNLPVAFAPFLNQLTVPILPA
CCEEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCEEEEECCCHHHHHHHCCCHHHHHH
HLLRNATPDQWQAWNQQPVGTGSFRFGKRDGNLIELLANTDYRPNIPNSRPNLERLEFRL
HHHCCCCCHHHHCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
YATMEAAHEALRRDLVDAFAYDVTEQGELPLPIGYRQVRAPLADYVVLTINLRRPPLDDL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHH
RVRQAIDYAVDPTTLITEVLQNRALPLNSPILPSSWAVAEGLTGYIDDAEQSRANGLLDQ
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHCCCHHH
AGWQRDAEGWRRKAGQLLRFDLVSANSPERSAIAERIQAQLATVGISSSLQLVPSSNLQD
HCCCCCHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHH
TLSQHTFDLAIHGWSNLGSDPDVYELWHSSQVNAANFAGLEDPAIDDLLVRARQTTSQET
HHHHCEEEEEEECCCCCCCCCCHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
RRELYAEFQAKWLALAPSVMLYQPLLEQQVGPSIEVLGLAPINQQAEVLYRHSDRFRRIA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
NWYLVSTRQVLPNFRNEPQNERPR
HHHEEEHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]