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The map label for this gene is pdhC [H]

Identifier: 159901148

GI number: 159901148

Start: 5924475

End: 5925803

Strand: Direct

Name: pdhC [H]

Synonym: Haur_4636

Alternate gene names: 159901148

Gene position: 5924475-5925803 (Clockwise)

Preceding gene: 159901147

Following gene: 159901152

Centisome position: 93.35

GC content: 54.63

Gene sequence:

>1329_bases
ATGGCGAAGAAACTAGAAATGCCCAAAATGGGCTACGATATGGTCGAAGGTACTTTGGCCAAATGGTTGAAAAAGCCAGG
CGATGAGGTTTCGCGTGGTGAACCAATTGCTGAAGTCGAAACCGATAAGGTCACGATTGAAATCGAGGCTTTTGAGGCTG
GGACAATCTTAAAGTTCTTGGTCAACGAAGGCGAAACCGTGCCAGTTGGTGCGCCAATCGCCGAAATTGACGATGGCTCA
GGCGATGACGAGGCCGAAGCAGCCAATGCCAGCGTTACGCCTTCCAGCGATGCTCCAGCAGTTGGCGAGGGTGGCGAGGC
CGCTCCGCCGGCTCCTGCCGTGGTCGCTCAACCAGAAAAAGTTGAGGCTACACCAGCAGCCAGTGCTCCGGCAACCAGCA
CTGGGCGCTTGTTTGCAACTCCAGCTGCTCGCGGTTTGGCCGAACAACGCGGCGTAGATTTGGCTGGCCTCAAGGGTTCT
GGCCCTGATGGCCGAATTGTTAAGGCCGATGTATTGGCTGCTGCCGTTGCACCAAAGGCTGCACCTGCTGCTACCCCAGC
CGCTGCGCCAGCTGCTGCACAAGCGGCTCCAGTTGCATCACCAGTGCCAGCACCAGTTGGCTTGATCTTCGCGCCACCAG
CACCAAATTCGGTCTACACCGAGGAGCCACTCTCGCGCTTACGCCAAACCGCTGCCAAGCGCATGGTCGAAAGCCAACAA
CAAGTGCCACCATTCTTCGTTACTTCAACGATTGAAATGGATGCGATTCAAGCCTTGTTGCCTAAGTTGCGTGAAGCTCA
TGGTGGCAAACTTTCAGTGACTGAATTGTTGCTGAAGGCTTGTGCTATCGCCTTGAAGAAGTTCCCCGCACTCAACTCGA
CCTTCGCTGGCGATAAGTTGTTGGTTCACAAAGATGTTCACATCAGCGTGGCTGTAGCAACCGATGCTGGCTTGTTGGCT
CCAGTCGTGCGCAACTGCGATAGCTTGAGCCTCGGCGCAATCTCCAACCAAATGCGCGATGTGATTGGCCGCACCCGCGA
TGGCAAAGCTGGCCTCGACGATCTCCAAGGCGGCACGTTTACCGTCAGCAACTTGGGGATGTTCGATGTCACCAACTTCA
TCGCGATTATCACGCCACCCCAAAGCGCAATTTTGGCAGTTGGCAGCACAATTGCCACTCCAGTTGTCCGCGATGGTGAA
ATTGTGATTCGTCAATTGATGAATGTCACGGTTTCAGCTGACCACCGCGCCACTGATGGAGCAAGCGTTGCCCAGTTCTT
GGTTGAACTCAAGAACTTGCTGCAAAACCCATTCAAGCTCTTGCTCTAA

Upstream 100 bases:

>100_bases
CGGCAGCAGTAGTTGAGGTTGGGTGGCTTGGCAGCAGGCTAAAGAAAACGGTTTCGTGAATTCTAAGCTTGTTGCCAAAC
CACCAACACACGAGGTCACG

Downstream 100 bases:

>100_bases
ATGTTTGAGCATAGATGAATCCCAGCTCCTTGCTTGCTTCGGCGGGCGAGGAGCTTTTTTTAATTATGAGGGATGAAGGC
GAAATCAAAAGGCAGAAGTC

Product: dehydrogenase catalytic domain-containing protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MAKKLEMPKMGYDMVEGTLAKWLKKPGDEVSRGEPIAEVETDKVTIEIEAFEAGTILKFLVNEGETVPVGAPIAEIDDGS
GDDEAEAANASVTPSSDAPAVGEGGEAAPPAPAVVAQPEKVEATPAASAPATSTGRLFATPAARGLAEQRGVDLAGLKGS
GPDGRIVKADVLAAAVAPKAAPAATPAAAPAAAQAAPVASPVPAPVGLIFAPPAPNSVYTEEPLSRLRQTAAKRMVESQQ
QVPPFFVTSTIEMDAIQALLPKLREAHGGKLSVTELLLKACAIALKKFPALNSTFAGDKLLVHKDVHISVAVATDAGLLA
PVVRNCDSLSLGAISNQMRDVIGRTRDGKAGLDDLQGGTFTVSNLGMFDVTNFIAIITPPQSAILAVGSTIATPVVRDGE
IVIRQLMNVTVSADHRATDGASVAQFLVELKNLLQNPFKLLL

Sequences:

>Translated_442_residues
MAKKLEMPKMGYDMVEGTLAKWLKKPGDEVSRGEPIAEVETDKVTIEIEAFEAGTILKFLVNEGETVPVGAPIAEIDDGS
GDDEAEAANASVTPSSDAPAVGEGGEAAPPAPAVVAQPEKVEATPAASAPATSTGRLFATPAARGLAEQRGVDLAGLKGS
GPDGRIVKADVLAAAVAPKAAPAATPAAAPAAAQAAPVASPVPAPVGLIFAPPAPNSVYTEEPLSRLRQTAAKRMVESQQ
QVPPFFVTSTIEMDAIQALLPKLREAHGGKLSVTELLLKACAIALKKFPALNSTFAGDKLLVHKDVHISVAVATDAGLLA
PVVRNCDSLSLGAISNQMRDVIGRTRDGKAGLDDLQGGTFTVSNLGMFDVTNFIAIITPPQSAILAVGSTIATPVVRDGE
IVIRQLMNVTVSADHRATDGASVAQFLVELKNLLQNPFKLLL
>Mature_441_residues
AKKLEMPKMGYDMVEGTLAKWLKKPGDEVSRGEPIAEVETDKVTIEIEAFEAGTILKFLVNEGETVPVGAPIAEIDDGSG
DDEAEAANASVTPSSDAPAVGEGGEAAPPAPAVVAQPEKVEATPAASAPATSTGRLFATPAARGLAEQRGVDLAGLKGSG
PDGRIVKADVLAAAVAPKAAPAATPAAAPAAAQAAPVASPVPAPVGLIFAPPAPNSVYTEEPLSRLRQTAAKRMVESQQQ
VPPFFVTSTIEMDAIQALLPKLREAHGGKLSVTELLLKACAIALKKFPALNSTFAGDKLLVHKDVHISVAVATDAGLLAP
VVRNCDSLSLGAISNQMRDVIGRTRDGKAGLDDLQGGTFTVSNLGMFDVTNFIAIITPPQSAILAVGSTIATPVVRDGEI
VIRQLMNVTVSADHRATDGASVAQFLVELKNLLQNPFKLLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=36.2612612612613, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=32.0175438596491, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=32.0175438596491, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI110671329, Length=445, Percent_Identity=29.8876404494382, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=34.6491228070175, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.6459627329193, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786946, Length=442, Percent_Identity=30.7692307692308, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786305, Length=456, Percent_Identity=29.8245614035088, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=458, Percent_Identity=35.589519650655, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17538894, Length=299, Percent_Identity=35.7859531772575, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=454, Percent_Identity=28.6343612334802, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=445, Percent_Identity=26.9662921348315, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=33.9869281045752, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6320352, Length=442, Percent_Identity=30.7692307692308, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321632, Length=183, Percent_Identity=32.2404371584699, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129315, Length=453, Percent_Identity=34.6578366445916, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24582497, Length=446, Percent_Identity=34.9775784753363, Blast_Score=198, Evalue=6e-51,
Organism=Drosophila melanogaster, GI18859875, Length=455, Percent_Identity=30.1098901098901, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24645909, Length=218, Percent_Identity=31.651376146789, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45536; Mature: 45404

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKLEMPKMGYDMVEGTLAKWLKKPGDEVSRGEPIAEVETDKVTIEIEAFEAGTILKFL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCEEECCEEEEEEEEECCCHHHHHH
VNEGETVPVGAPIAEIDDGSGDDEAEAANASVTPSSDAPAVGEGGEAAPPAPAVVAQPEK
HCCCCEEECCCCHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
VEATPAASAPATSTGRLFATPAARGLAEQRGVDLAGLKGSGPDGRIVKADVLAAAVAPKA
CCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCEECCCCCCCCCCEEEHHHHHHHHCCCC
APAATPAAAPAAAQAAPVASPVPAPVGLIFAPPAPNSVYTEEPLSRLRQTAAKRMVESQQ
CCCCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QVPPFFVTSTIEMDAIQALLPKLREAHGGKLSVTELLLKACAIALKKFPALNSTFAGDKL
CCCCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LVHKDVHISVAVATDAGLLAPVVRNCDSLSLGAISNQMRDVIGRTRDGKAGLDDLQGGTF
EEEECCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCEE
TVSNLGMFDVTNFIAIITPPQSAILAVGSTIATPVVRDGEIVIRQLMNVTVSADHRATDG
EECCCCHHHHCCEEEEEECCCHHHHHHCHHHHCCCCCCHHHHHHHHHHCEECCCCCCCCH
ASVAQFLVELKNLLQNPFKLLL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKLEMPKMGYDMVEGTLAKWLKKPGDEVSRGEPIAEVETDKVTIEIEAFEAGTILKFL
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCEEECCEEEEEEEEECCCHHHHHH
VNEGETVPVGAPIAEIDDGSGDDEAEAANASVTPSSDAPAVGEGGEAAPPAPAVVAQPEK
HCCCCEEECCCCHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
VEATPAASAPATSTGRLFATPAARGLAEQRGVDLAGLKGSGPDGRIVKADVLAAAVAPKA
CCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCEECCCCCCCCCCEEEHHHHHHHHCCCC
APAATPAAAPAAAQAAPVASPVPAPVGLIFAPPAPNSVYTEEPLSRLRQTAAKRMVESQQ
CCCCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QVPPFFVTSTIEMDAIQALLPKLREAHGGKLSVTELLLKACAIALKKFPALNSTFAGDKL
CCCCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LVHKDVHISVAVATDAGLLAPVVRNCDSLSLGAISNQMRDVIGRTRDGKAGLDDLQGGTF
EEEECCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCEE
TVSNLGMFDVTNFIAIITPPQSAILAVGSTIATPVVRDGEIVIRQLMNVTVSADHRATDG
EECCCCHHHHCCEEEEEECCCHHHHHHCHHHHCCCCCCHHHHHHHHHHCEECCCCCCCCH
ASVAQFLVELKNLLQNPFKLLL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA