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The map label for this gene is pdhB [H]

Identifier: 159901147

GI number: 159901147

Start: 5923388

End: 5924386

Strand: Direct

Name: pdhB [H]

Synonym: Haur_4635

Alternate gene names: 159901147

Gene position: 5923388-5924386 (Clockwise)

Preceding gene: 159901146

Following gene: 159901148

Centisome position: 93.33

GC content: 50.95

Gene sequence:

>999_bases
ATGCCCGTTATAACCTACTCGGAAGCCTTGCGCCAAGCATTGCGCGAAGCAATGACCAACGATCCACGGGTGTTTATCAT
TGGTGAAGATGTTGTTCACTACGATAGCGCTTACGGTGTCACCAAAGGGTTTGAAAAAGAATTCGGCCCCGAACGCATCA
AAGATATGCCAATTGCTGAAGCTGGGTATGCTGGTTTAGGCATCGGCGCTGCGATGAACGGCCTGCGCCCGATCGTGGAA
ATGATGACCACCAACTTTGCAATTTTGGCGCTGGATATGATTATCAACCACGCTGCTAAATTGCACTATATGTTCGGTGG
TCAATTTACCTGCCCAATCGTGTTCCGCATGCCCAATGGCTATGGTCAATTGAGCGCCACCCACTCGCAAGCCTTCGATA
ACTACTATGCCTACATGCCAGGCTTGAAAGTGGTTGTGCCTGGCACTCCATACGATGCCAAAGGCTTGATGAAGGCCGCG
ATCGAAGATCCCGATCCCGTGATTTTCATCGAACACACTGGGATCTACAACATCAAGGGCGAAGTGCCAGAAGAAAGCTA
CACCGTTCCGATTGGCAAATCGAACTTGTTGCGCGATGGCAAAGATGTGACGATTGTGGGCTATGGCCGCATGATTCCTT
ACTGCCAACAAGCCGTTGAAACCTTGGCAAGCGAAGGCATCGATGCAGCCTTGGTTGACTTGCGCACCATTCGTCCGCTC
GATATGGAGCCAGTCTTGGAAAGTTTCCGCAAAACCAACCGCGCTGTAATTGCCACCGAAGAGTGGACATCAGTTGGTGT
TGGCTCGGAAATTGCCGCTCGCTTGTATACCGAAGGCTTTGATCACTTGGATGCTCCAATTTGGCGCGTGGGCTTTGACG
AAGTGCCAATGCCGTATGCCAAAAACTTGGAAGCCCATGTGGTTCCCAATGCCGATTCAGTCATTCAAGCAGTCAAGAAT
GTGCTGGCAGGCAAGACCCAAAAGATTCGGCAGCAGTAG

Upstream 100 bases:

>100_bases
GAAATGGATGTAGTGGTGCAATTTGCCGAAGAAAGCCCTGCCCCAGACCTGAGCGAAGCATGGACCGAAATCTATTCGAA
GCCGCTCTAAGGAGATATCA

Downstream 100 bases:

>100_bases
TTGAGGTTGGGTGGCTTGGCAGCAGGCTAAAGAAAACGGTTTCGTGAATTCTAAGCTTGTTGCCAAACCACCAACACACG
AGGTCACGATGGCGAAGAAA

Product: transketolase central region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MPVITYSEALRQALREAMTNDPRVFIIGEDVVHYDSAYGVTKGFEKEFGPERIKDMPIAEAGYAGLGIGAAMNGLRPIVE
MMTTNFAILALDMIINHAAKLHYMFGGQFTCPIVFRMPNGYGQLSATHSQAFDNYYAYMPGLKVVVPGTPYDAKGLMKAA
IEDPDPVIFIEHTGIYNIKGEVPEESYTVPIGKSNLLRDGKDVTIVGYGRMIPYCQQAVETLASEGIDAALVDLRTIRPL
DMEPVLESFRKTNRAVIATEEWTSVGVGSEIAARLYTEGFDHLDAPIWRVGFDEVPMPYAKNLEAHVVPNADSVIQAVKN
VLAGKTQKIRQQ

Sequences:

>Translated_332_residues
MPVITYSEALRQALREAMTNDPRVFIIGEDVVHYDSAYGVTKGFEKEFGPERIKDMPIAEAGYAGLGIGAAMNGLRPIVE
MMTTNFAILALDMIINHAAKLHYMFGGQFTCPIVFRMPNGYGQLSATHSQAFDNYYAYMPGLKVVVPGTPYDAKGLMKAA
IEDPDPVIFIEHTGIYNIKGEVPEESYTVPIGKSNLLRDGKDVTIVGYGRMIPYCQQAVETLASEGIDAALVDLRTIRPL
DMEPVLESFRKTNRAVIATEEWTSVGVGSEIAARLYTEGFDHLDAPIWRVGFDEVPMPYAKNLEAHVVPNADSVIQAVKN
VLAGKTQKIRQQ
>Mature_331_residues
PVITYSEALRQALREAMTNDPRVFIIGEDVVHYDSAYGVTKGFEKEFGPERIKDMPIAEAGYAGLGIGAAMNGLRPIVEM
MTTNFAILALDMIINHAAKLHYMFGGQFTCPIVFRMPNGYGQLSATHSQAFDNYYAYMPGLKVVVPGTPYDAKGLMKAAI
EDPDPVIFIEHTGIYNIKGEVPEESYTVPIGKSNLLRDGKDVTIVGYGRMIPYCQQAVETLASEGIDAALVDLRTIRPLD
MEPVLESFRKTNRAVIATEEWTSVGVGSEIAARLYTEGFDHLDAPIWRVGFDEVPMPYAKNLEAHVVPNADSVIQAVKNV
LAGKTQKIRQQ

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=45.0920245398773, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=42.4615384615385, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI4557353, Length=320, Percent_Identity=33.75, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI34101272, Length=320, Percent_Identity=33.75, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1786622, Length=229, Percent_Identity=26.6375545851528, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=325, Percent_Identity=46.1538461538462, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17506935, Length=319, Percent_Identity=36.3636363636364, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=44.954128440367, Blast_Score=290, Evalue=3e-79,
Organism=Drosophila melanogaster, GI21358145, Length=331, Percent_Identity=45.9214501510574, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24650940, Length=331, Percent_Identity=45.9214501510574, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI160714832, Length=319, Percent_Identity=34.7962382445141, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI160714828, Length=319, Percent_Identity=34.7962382445141, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=49.438202247191, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=49.438202247191, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36582; Mature: 36451

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITYSEALRQALREAMTNDPRVFIIGEDVVHYDSAYGVTKGFEKEFGPERIKDMPIAE
CCEEEHHHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCCCCCHHHHCCHHHHHCCCCCC
AGYAGLGIGAAMNGLRPIVEMMTTNFAILALDMIINHAAKLHYMFGGQFTCPIVFRMPNG
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCEEECEEEEECCCC
YGQLSATHSQAFDNYYAYMPGLKVVVPGTPYDAKGLMKAAIEDPDPVIFIEHTGIYNIKG
CCCCCCHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCEEEECC
EVPEESYTVPIGKSNLLRDGKDVTIVGYGRMIPYCQQAVETLASEGIDAALVDLRTIRPL
CCCCCCEEECCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCC
DMEPVLESFRKTNRAVIATEEWTSVGVGSEIAARLYTEGFDHLDAPIWRVGFDEVPMPYA
CHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHCCCEEECCCCCCCCCHH
KNLEAHVVPNADSVIQAVKNVLAGKTQKIRQQ
CCCCEEECCCHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure 
PVITYSEALRQALREAMTNDPRVFIIGEDVVHYDSAYGVTKGFEKEFGPERIKDMPIAE
CEEEHHHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCCCCCHHHHCCHHHHHCCCCCC
AGYAGLGIGAAMNGLRPIVEMMTTNFAILALDMIINHAAKLHYMFGGQFTCPIVFRMPNG
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCEEECEEEEECCCC
YGQLSATHSQAFDNYYAYMPGLKVVVPGTPYDAKGLMKAAIEDPDPVIFIEHTGIYNIKG
CCCCCCHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCEEEECC
EVPEESYTVPIGKSNLLRDGKDVTIVGYGRMIPYCQQAVETLASEGIDAALVDLRTIRPL
CCCCCCEEECCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCC
DMEPVLESFRKTNRAVIATEEWTSVGVGSEIAARLYTEGFDHLDAPIWRVGFDEVPMPYA
CHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHCCCEEECCCCCCCCCHH
KNLEAHVVPNADSVIQAVKNVLAGKTQKIRQQ
CCCCEEECCCHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]