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The map label for this gene is lpdA [H]

Identifier: 159901026

GI number: 159901026

Start: 5779011

End: 5780408

Strand: Reverse

Name: lpdA [H]

Synonym: Haur_4513

Alternate gene names: 159901026

Gene position: 5780408-5779011 (Counterclockwise)

Preceding gene: 159901027

Following gene: 159901025

Centisome position: 91.08

GC content: 50.79

Gene sequence:

>1398_bases
ATGGCTCAATATGATGTTGTGATTATTGGTTCAGGGCCAGGCGGTGAAGTTGCTGCCTTACGCGCTGTCCAATTGGGCTT
AAAAGTCGCTGTCGTCGAAAAAGAACAAGTCGGCGGTGTCTGTTTAAATATTGGCTGTATTCCTACCAAATCGCTGTTGC
ACTGCGCCGACTTGCTGGAAGAAACCAAAGAAGGCAAGAAGTTTGGCGTAATCACGGGCGAAGTTTCGTTCGATCTCAAG
GGTGCCATGGGCCACAAAGAAAAAGTGGTCAAAACCATGCGCACTGGTCTCGAAGGCTTGTTCAAAAAGAAGAAAATCGA
TCTCTTTCGCGGGTTTGGCCGTTTGGCTGGCACCAACAAGGTCGCGGTCAAAGGCAACGATGGTACTGAAACGGTCATCG
AAACCAAGAATGTGATTTTGGCAGTTGGCTCGACTCCTCGGGCATTGCCATTCGCGCCATTCGACGAGCAACGGATCGTT
TCGAGCACTGGAGCCTTGAGCTTGCCCGAAGTGCCAAAACATTTGGTGGTAATCGGCGGTGGGATCATCGGCTGCGAATT
TGCTTCGATGTATCGTACCTTCGGCTCGCAAGTGAGCATCATCGAAATGTTGCCACGAATTGTTGCCACTGAGGATGAAG
AAATTTCGGCTGAATTGGCCAAAGCCTTCACCAAACGCGGGATCAAGCTCTACACTGGCTCGAAAACCAAGGCCGTCAAC
AAACGTGCCGATGGTGCTAGCGTGGTTTTTGAAGGTCCAGATGGCAAAGAACAAACTTTAGAAGCAGATTACGTCCTGAT
TGGCACAGGTCGCGCTCCTTTGACCAAAAATATTGGCCTCGAAGAAGTCGGCGTAACCATCGATGAACGTGGCTATATTC
CAGTCAATGCCACCATGCAAACCAACATTGCCAATATTTATGCAATTGGCGATGTTGTGCCAACGCCATGGCTGGCTCAC
GTTGCCTCAGCCGAAGGCGTGATTGCGGTCGAACATATCGCAGGCCACCATACCAACCCAATCAACTACGACATTATTCC
AAGCTGTGTCTATTGTTCGCCCGAAGTGGCGCACGTTGGCTTGACCGAAGCCCAAGCCAAAGAACGCGGCTACAATGTCA
AAGTTGGCAAGTTTCCATTTGCGGCGGTTGGCAAAGCTACAGCCATTGGCAATCGCGATGGCTTTGTTAAAATCGTTGCC
GACGCTGAATATGGCGAGATCCTTGGCTTCCATATGATTGGGCCTCGTGTGACCGAGTTGGTTGCCGAAGGCGGCTTGGC
TTTGAGCCACGAAGCCACGGTCGAATCGCTGTTAGCAACGATTCATGCTCACCCAACCTTATACGAAGCCATGCACGAAG
CTGGTCATGCCTTGCTTGACGGCACACTTCACCTCTAA

Upstream 100 bases:

>100_bases
TTGCCTTCTGACTTTTGACTTTTGATTTCTGCCTAAACCACTGCCTGTGCTATCATAGACAATGGACAAAACGTCTACCA
TACGGAGGATTGATATTTCA

Downstream 100 bases:

>100_bases
ATTAAATTGCGCTGAATCAGCTGACGAAGGACACGAAGCACCATTGCAGGTGTTTTGCGTCCTTCGTGATTATTGAAGCC
TTGGTTGCTATGAAAATTTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAQYDVVIIGSGPGGEVAALRAVQLGLKVAVVEKEQVGGVCLNIGCIPTKSLLHCADLLEETKEGKKFGVITGEVSFDLK
GAMGHKEKVVKTMRTGLEGLFKKKKIDLFRGFGRLAGTNKVAVKGNDGTETVIETKNVILAVGSTPRALPFAPFDEQRIV
SSTGALSLPEVPKHLVVIGGGIIGCEFASMYRTFGSQVSIIEMLPRIVATEDEEISAELAKAFTKRGIKLYTGSKTKAVN
KRADGASVVFEGPDGKEQTLEADYVLIGTGRAPLTKNIGLEEVGVTIDERGYIPVNATMQTNIANIYAIGDVVPTPWLAH
VASAEGVIAVEHIAGHHTNPINYDIIPSCVYCSPEVAHVGLTEAQAKERGYNVKVGKFPFAAVGKATAIGNRDGFVKIVA
DAEYGEILGFHMIGPRVTELVAEGGLALSHEATVESLLATIHAHPTLYEAMHEAGHALLDGTLHL

Sequences:

>Translated_465_residues
MAQYDVVIIGSGPGGEVAALRAVQLGLKVAVVEKEQVGGVCLNIGCIPTKSLLHCADLLEETKEGKKFGVITGEVSFDLK
GAMGHKEKVVKTMRTGLEGLFKKKKIDLFRGFGRLAGTNKVAVKGNDGTETVIETKNVILAVGSTPRALPFAPFDEQRIV
SSTGALSLPEVPKHLVVIGGGIIGCEFASMYRTFGSQVSIIEMLPRIVATEDEEISAELAKAFTKRGIKLYTGSKTKAVN
KRADGASVVFEGPDGKEQTLEADYVLIGTGRAPLTKNIGLEEVGVTIDERGYIPVNATMQTNIANIYAIGDVVPTPWLAH
VASAEGVIAVEHIAGHHTNPINYDIIPSCVYCSPEVAHVGLTEAQAKERGYNVKVGKFPFAAVGKATAIGNRDGFVKIVA
DAEYGEILGFHMIGPRVTELVAEGGLALSHEATVESLLATIHAHPTLYEAMHEAGHALLDGTLHL
>Mature_464_residues
AQYDVVIIGSGPGGEVAALRAVQLGLKVAVVEKEQVGGVCLNIGCIPTKSLLHCADLLEETKEGKKFGVITGEVSFDLKG
AMGHKEKVVKTMRTGLEGLFKKKKIDLFRGFGRLAGTNKVAVKGNDGTETVIETKNVILAVGSTPRALPFAPFDEQRIVS
STGALSLPEVPKHLVVIGGGIIGCEFASMYRTFGSQVSIIEMLPRIVATEDEEISAELAKAFTKRGIKLYTGSKTKAVNK
RADGASVVFEGPDGKEQTLEADYVLIGTGRAPLTKNIGLEEVGVTIDERGYIPVNATMQTNIANIYAIGDVVPTPWLAHV
ASAEGVIAVEHIAGHHTNPINYDIIPSCVYCSPEVAHVGLTEAQAKERGYNVKVGKFPFAAVGKATAIGNRDGFVKIVAD
AEYGEILGFHMIGPRVTELVAEGGLALSHEATVESLLATIHAHPTLYEAMHEAGHALLDGTLHL

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=44.5652173913043, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=27.4089935760171, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI148277065, Length=472, Percent_Identity=28.6016949152542, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI33519430, Length=472, Percent_Identity=28.6016949152542, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33519428, Length=472, Percent_Identity=28.6016949152542, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33519426, Length=472, Percent_Identity=28.6016949152542, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI148277071, Length=472, Percent_Identity=28.6016949152542, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI291045266, Length=429, Percent_Identity=26.8065268065268, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI22035672, Length=469, Percent_Identity=27.7185501066098, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=24.7058823529412, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI226437568, Length=283, Percent_Identity=25.4416961130742, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI21389617, Length=283, Percent_Identity=25.4416961130742, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI65787454, Length=283, Percent_Identity=25.4416961130742, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=41.5384615384615, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI87082354, Length=451, Percent_Identity=30.3769401330377, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=30.9468822170901, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=27.292576419214, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=42.1739130434783, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=25.9493670886076, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=374, Percent_Identity=29.4117647058824, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=372, Percent_Identity=29.5698924731183, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=24.7401247401247, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=194, Percent_Identity=31.4432989690722, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=41.3502109704641, Blast_Score=333, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=26.7223382045929, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=26.3498920086393, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=45.5142231947484, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=30.0847457627119, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640553, Length=472, Percent_Identity=30.0847457627119, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640551, Length=472, Percent_Identity=30.0847457627119, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=28.1837160751566, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49294; Mature: 49163

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQYDVVIIGSGPGGEVAALRAVQLGLKVAVVEKEQVGGVCLNIGCIPTKSLLHCADLLE
CCCEEEEEEECCCCCCHHHHHHHHHCEEEEEEEHHCCCCEEEEECCCCCHHHHHHHHHHH
ETKEGKKFGVITGEVSFDLKGAMGHKEKVVKTMRTGLEGLFKKKKIDLFRGFGRLAGTNK
HHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VAVKGNDGTETVIETKNVILAVGSTPRALPFAPFDEQRIVSSTGALSLPEVPKHLVVIGG
EEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEC
GIIGCEFASMYRTFGSQVSIIEMLPRIVATEDEEISAELAKAFTKRGIKLYTGSKTKAVN
CEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHC
KRADGASVVFEGPDGKEQTLEADYVLIGTGRAPLTKNIGLEEVGVTIDERGYIPVNATMQ
CCCCCCEEEEECCCCCCCEEECCEEEEECCCCCCCCCCCHHHHCEEECCCCEEEECCEEE
TNIANIYAIGDVVPTPWLAHVASAEGVIAVEHIAGHHTNPINYDIIPSCVYCSPEVAHVG
CCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEECC
LTEAQAKERGYNVKVGKFPFAAVGKATAIGNRDGFVKIVADAEYGEILGFHMIGPRVTEL
CCHHHHHHCCCEEEECCCCCHHCCCEEEECCCCCEEEEEECCCCCCEEEEEECCHHHHHH
VAEGGLALSHEATVESLLATIHAHPTLYEAMHEAGHALLDGTLHL
HHCCCEEECHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCEECC
>Mature Secondary Structure 
AQYDVVIIGSGPGGEVAALRAVQLGLKVAVVEKEQVGGVCLNIGCIPTKSLLHCADLLE
CCEEEEEEECCCCCCHHHHHHHHHCEEEEEEEHHCCCCEEEEECCCCCHHHHHHHHHHH
ETKEGKKFGVITGEVSFDLKGAMGHKEKVVKTMRTGLEGLFKKKKIDLFRGFGRLAGTNK
HHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VAVKGNDGTETVIETKNVILAVGSTPRALPFAPFDEQRIVSSTGALSLPEVPKHLVVIGG
EEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEC
GIIGCEFASMYRTFGSQVSIIEMLPRIVATEDEEISAELAKAFTKRGIKLYTGSKTKAVN
CEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHC
KRADGASVVFEGPDGKEQTLEADYVLIGTGRAPLTKNIGLEEVGVTIDERGYIPVNATMQ
CCCCCCEEEEECCCCCCCEEECCEEEEECCCCCCCCCCCHHHHCEEECCCCEEEECCEEE
TNIANIYAIGDVVPTPWLAHVASAEGVIAVEHIAGHHTNPINYDIIPSCVYCSPEVAHVG
CCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEECC
LTEAQAKERGYNVKVGKFPFAAVGKATAIGNRDGFVKIVADAEYGEILGFHMIGPRVTEL
CCHHHHHHCCCEEEECCCCCHHCCCEEEECCCCCEEEEEECCCCCCEEEEEECCHHHHHH
VAEGGLALSHEATVESLLATIHAHPTLYEAMHEAGHALLDGTLHL
HHCCCEEECHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]