Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 159900959

GI number: 159900959

Start: 5687284

End: 5688951

Strand: Reverse

Name: leuA [H]

Synonym: Haur_4446

Alternate gene names: 159900959

Gene position: 5688951-5687284 (Counterclockwise)

Preceding gene: 159900960

Following gene: 159900958

Centisome position: 89.64

GC content: 52.34

Gene sequence:

>1668_bases
ATGACCAGCGATTATGTACGCATTTTCGATACAACCCTGCGCGACGGTGAGCAATCACCAGGCTGTACCATGCATCTGCA
CGAAAAATTGGAAGTTGCCAAGCAATTGGCGCGAATGGGCGTGGATATTATCGAAGCGGGCTTTCCGGCGGCCTCACCTG
GCGATTGGGAAGCAGTTAACCAAATTGCCAAGAGCGTTGGCACTGCCGATGGTCCAGTGATCTGTGGCTTAGCACGAGCA
GTCAAAAGCGATATCGAAGCCTGTGCCACAGCGATTGCACCTGCCGCCAAAAAGCGTATTCACACTTTCCTTTCAAGCTC
AGATATTCATATCGAGTATCAATTGCGCTCAACCCGCGAGGAAGTGCTGACGAAAACCCGTGAGATGGTGCGCTTGGCTA
GCTCGCTTTGCGACGATGTTGAATTCTCGCCGATGGATGCGACCCGTTCCGACATCGAATTTATGTATCAGATGCTGGCG
ATAGCGATTGAAGAAGGCGCAACAACCTTGAATATTCCTGATACCGTGGGCTATTCCACGCCTGAGGAATATGCAGCGTT
GCTTCGGGGGATTATCGAAAACGTACCTGGTGCTGCTGATGTGATTATCTCAACCCATTGCCACGACGATTTGGGCTTGG
CGGTGGCGAATTCGTTGGCTGGCGTTCGAGCTGGCGCACGCCAAATTGAATGCACAATCAATGGCATCGGCGAGCGAGCT
GGCAATGCTTCGTTGGAAGAAGTGGTGATGGCTTTGGAAACGCGCAAGCAATTTTACGGCCTGAGCACCAATATCGACAC
AACCCAACTAACGCCTAGCTCACGCTTGTTGAGTGCCTGCACCAACACCCAAGTGCCACCCAACAAGGCAATTGTTGGCG
CGAATGCCTTTGCTCACGAATCGGGCATTCACCAAGATGGCGTGTTGAAACACCGTATGACCTACGAAATTATGAGCGCC
GAATCGGTGGGTCAAGATGGCAATGCCTTGGTTTTGGGCAAACACTCAGGCCGCCATGCCTTCCGCCATCGGGTGCAAGA
ATTGGGCTATAGCTTCGATGAAGCTACGATCAACCACTTGTTTGCCCGCTTCAAAGATGTAGCTGATCGCAAGAAATATG
TTGATGATCGCGATGTTGAGGCCTTGATTAGCGATGAAGCAGGCCGCCCAAGCCCAGTTTATGAATTGGAGCATGTGCAA
TTTGCCTCAGGCGTGAACGCCATCCCAACCGCAACCGTGCGCATGCGCGGCCCCAACGGCGAAGTCAAAATCGAATCAGC
CCAAGGCACAGGCCCAGTGGATGCAGTCTATTCGGCGATCAACAAAGTTGTGCTAACTCCGGTTACGTTGCTCGAATTTG
CGGTCAATGCGATCACCGAAGGTATCGATGCGGTTGGCGAAGTTAGCGTGAAAGTCGTCGAAGGCAAGCATAAACATGTG
CGTGGAGTTTTAACCGACAAAGCAACAGATGCCAAAGTTTGGCGCGGCAATGGAGTCAATGTTGATATCATCGTTGCGGC
TGCCGAAGCCTATGTTAGCGCCTTGAACAAATTGCTGAAAGCTCGCCAAGAACGGCTCAAGCAAGAAACCACCGCCCGCA
TGGAAGTTGCCAGCGCTGCGCCAGGCGTGGATTTGTTCGGTGGCTCGACCCTTGGACGGTATGAATAA

Upstream 100 bases:

>100_bases
GAGGCGAGGGGGAATTGCTTAGGCTAACTCCCCTCTCCCACGCAATGGGAGAGGGGCTGGGGGTGAGGGAATAAAGCTAG
CCCTCATTACGGAGAACTTT

Downstream 100 bases:

>100_bases
TTGCTGAGGTTGGGTCAAATCAACCTCGCTAGCCCAAAGAACTTATGAAAATTGCTTATCTTGGCCCGGCTGGTACATTC
AGCGAAAAAGCAGCGTTGGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTSDYVRIFDTTLRDGEQSPGCTMHLHEKLEVAKQLARMGVDIIEAGFPAASPGDWEAVNQIAKSVGTADGPVICGLARA
VKSDIEACATAIAPAAKKRIHTFLSSSDIHIEYQLRSTREEVLTKTREMVRLASSLCDDVEFSPMDATRSDIEFMYQMLA
IAIEEGATTLNIPDTVGYSTPEEYAALLRGIIENVPGAADVIISTHCHDDLGLAVANSLAGVRAGARQIECTINGIGERA
GNASLEEVVMALETRKQFYGLSTNIDTTQLTPSSRLLSACTNTQVPPNKAIVGANAFAHESGIHQDGVLKHRMTYEIMSA
ESVGQDGNALVLGKHSGRHAFRHRVQELGYSFDEATINHLFARFKDVADRKKYVDDRDVEALISDEAGRPSPVYELEHVQ
FASGVNAIPTATVRMRGPNGEVKIESAQGTGPVDAVYSAINKVVLTPVTLLEFAVNAITEGIDAVGEVSVKVVEGKHKHV
RGVLTDKATDAKVWRGNGVNVDIIVAAAEAYVSALNKLLKARQERLKQETTARMEVASAAPGVDLFGGSTLGRYE

Sequences:

>Translated_555_residues
MTSDYVRIFDTTLRDGEQSPGCTMHLHEKLEVAKQLARMGVDIIEAGFPAASPGDWEAVNQIAKSVGTADGPVICGLARA
VKSDIEACATAIAPAAKKRIHTFLSSSDIHIEYQLRSTREEVLTKTREMVRLASSLCDDVEFSPMDATRSDIEFMYQMLA
IAIEEGATTLNIPDTVGYSTPEEYAALLRGIIENVPGAADVIISTHCHDDLGLAVANSLAGVRAGARQIECTINGIGERA
GNASLEEVVMALETRKQFYGLSTNIDTTQLTPSSRLLSACTNTQVPPNKAIVGANAFAHESGIHQDGVLKHRMTYEIMSA
ESVGQDGNALVLGKHSGRHAFRHRVQELGYSFDEATINHLFARFKDVADRKKYVDDRDVEALISDEAGRPSPVYELEHVQ
FASGVNAIPTATVRMRGPNGEVKIESAQGTGPVDAVYSAINKVVLTPVTLLEFAVNAITEGIDAVGEVSVKVVEGKHKHV
RGVLTDKATDAKVWRGNGVNVDIIVAAAEAYVSALNKLLKARQERLKQETTARMEVASAAPGVDLFGGSTLGRYE
>Mature_554_residues
TSDYVRIFDTTLRDGEQSPGCTMHLHEKLEVAKQLARMGVDIIEAGFPAASPGDWEAVNQIAKSVGTADGPVICGLARAV
KSDIEACATAIAPAAKKRIHTFLSSSDIHIEYQLRSTREEVLTKTREMVRLASSLCDDVEFSPMDATRSDIEFMYQMLAI
AIEEGATTLNIPDTVGYSTPEEYAALLRGIIENVPGAADVIISTHCHDDLGLAVANSLAGVRAGARQIECTINGIGERAG
NASLEEVVMALETRKQFYGLSTNIDTTQLTPSSRLLSACTNTQVPPNKAIVGANAFAHESGIHQDGVLKHRMTYEIMSAE
SVGQDGNALVLGKHSGRHAFRHRVQELGYSFDEATINHLFARFKDVADRKKYVDDRDVEALISDEAGRPSPVYELEHVQF
ASGVNAIPTATVRMRGPNGEVKIESAQGTGPVDAVYSAINKVVLTPVTLLEFAVNAITEGIDAVGEVSVKVVEGKHKHVR
GVLTDKATDAKVWRGNGVNVDIIVAAAEAYVSALNKLLKARQERLKQETTARMEVASAAPGVDLFGGSTLGRYE

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=521, Percent_Identity=45.2975047984645, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6324682, Length=368, Percent_Identity=29.6195652173913, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=364, Percent_Identity=29.1208791208791, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320019, Length=385, Percent_Identity=26.4935064935065, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320071, Length=388, Percent_Identity=25.7731958762887, Blast_Score=121, Evalue=4e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59740; Mature: 59609

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDYVRIFDTTLRDGEQSPGCTMHLHEKLEVAKQLARMGVDIIEAGFPAASPGDWEAVN
CCCCCEEEHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHH
QIAKSVGTADGPVICGLARAVKSDIEACATAIAPAAKKRIHTFLSSSDIHIEYQLRSTRE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHH
EVLTKTREMVRLASSLCDDVEFSPMDATRSDIEFMYQMLAIAIEEGATTLNIPDTVGYST
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PEEYAALLRGIIENVPGAADVIISTHCHDDLGLAVANSLAGVRAGARQIECTINGIGERA
HHHHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
GNASLEEVVMALETRKQFYGLSTNIDTTQLTPSSRLLSACTNTQVPPNKAIVGANAFAHE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHHHC
SGIHQDGVLKHRMTYEIMSAESVGQDGNALVLGKHSGRHAFRHRVQELGYSFDEATINHL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
FARFKDVADRKKYVDDRDVEALISDEAGRPSPVYELEHVQFASGVNAIPTATVRMRGPNG
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCC
EVKIESAQGTGPVDAVYSAINKVVLTPVTLLEFAVNAITEGIDAVGEVSVKVVEGKHKHV
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHH
RGVLTDKATDAKVWRGNGVNVDIIVAAAEAYVSALNKLLKARQERLKQETTARMEVASAA
HEECCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGVDLFGGSTLGRYE
CCCEECCCCCCCCCC
>Mature Secondary Structure 
TSDYVRIFDTTLRDGEQSPGCTMHLHEKLEVAKQLARMGVDIIEAGFPAASPGDWEAVN
CCCCEEEHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHH
QIAKSVGTADGPVICGLARAVKSDIEACATAIAPAAKKRIHTFLSSSDIHIEYQLRSTRE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHH
EVLTKTREMVRLASSLCDDVEFSPMDATRSDIEFMYQMLAIAIEEGATTLNIPDTVGYST
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PEEYAALLRGIIENVPGAADVIISTHCHDDLGLAVANSLAGVRAGARQIECTINGIGERA
HHHHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
GNASLEEVVMALETRKQFYGLSTNIDTTQLTPSSRLLSACTNTQVPPNKAIVGANAFAHE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHHHC
SGIHQDGVLKHRMTYEIMSAESVGQDGNALVLGKHSGRHAFRHRVQELGYSFDEATINHL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
FARFKDVADRKKYVDDRDVEALISDEAGRPSPVYELEHVQFASGVNAIPTATVRMRGPNG
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCC
EVKIESAQGTGPVDAVYSAINKVVLTPVTLLEFAVNAITEGIDAVGEVSVKVVEGKHKHV
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHH
RGVLTDKATDAKVWRGNGVNVDIIVAAAEAYVSALNKLLKARQERLKQETTARMEVASAA
HEECCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGVDLFGGSTLGRYE
CCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA