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The map label for this gene is xylF [H]

Identifier: 159900853

GI number: 159900853

Start: 5547890

End: 5549014

Strand: Reverse

Name: xylF [H]

Synonym: Haur_4340

Alternate gene names: 159900853

Gene position: 5549014-5547890 (Counterclockwise)

Preceding gene: 159900855

Following gene: 159900852

Centisome position: 87.43

GC content: 50.76

Gene sequence:

>1125_bases
ATGAACGTGCGGCGTAGCGCATTCACTTCCCTCTTGTTGATCCTCATCTCTAGCGTGATTGCAGCCTGTGGTAGCGAAAG
CGCTACAACTGCTCCAACCACAGGCACTGGTGGCACAACCAGCAGTGGCGGCAAAGTAGTCGCTTTGTTCTTGCCCGATG
CCAAAACTGCCCGCTATGAAACTGCCGACCGCCCCTACTTCGAAGCCAAAATGAAAGAGCTTTGCCCAGATTGTCAGGTG
ATTTACAACAACGCCAACCAAGATGCCAGCTTGCAATTGCAACAAGCTGAAGCAGCGTTGACCAACGGCGCAAAGGTTTT
GGTGCTTGACCCAGTTGATTCTGCTGCTGCTGCTTCGATTGCCGACAAAGCCAAAGCCCAAAATGTGCCAGTCATCGCCT
ACGACCGCTTGATCCTTAACTCGGATGGCGTGAGCTACTACATTTCATTCGACAACGAATCAGTTGGCAAGTTGCAAGCC
GAAAGCTTGGTTGCGCAATTAGACAAGCAAGGGATTGCCAACCCAACTATCGTCATGATCAACGGCTCACCAACCGACAA
CAATGCTAAATTGTTCAAAGCTGGCGCTCACAGCGTGTTTGATCCATTGGTTAGCGCTGGCAAATTGACCATCGCCAACG
AATATGACACTCCCGACTGGAGCCCCGACAAAGCCCAAGACCAAATGCAACAAGCCTTGACCAGCATGGGTAACAAAGTT
GATGGCGTATATGCTGCCAACGACGGTACTGGTGGCGGGGCGATTGCCGCTATGAAGGCTGGTGGTCTCTCACCATTGCC
TCCAGTCACAGGCCAAGATGCTGAATTGGCGGCGATTCAACGGATTTTGGCTGGCGATCAATACATGACCGTGTACAAAG
CGATCAAGCCACAAGCCGAAGCTGCTGCTGAATTGGCCTTTGCCTTGCTCGAAGGCAAAACCAGCGACAAAGCTACCAGC
AAAGTCAACAATGGCAAAATTGATGTTCCTTCAATCTTGCTGACCCCAATTGCTGTGACCAAAGAAAACGTCAAAGACAC
GATTGTCAAAGACCAATTCCACAAAGTTGATCAGATCTGTGCTGGCGACTTCGCCAAAGCCTGTGCTGATGCCGGTATTC
AATAA

Upstream 100 bases:

>100_bases
GCTCACCGGGAGCAGGTTCGTTCGATTTGGTTGGCTTTTTGTTCGCACAATTCGGTATCAATGACGATTGTAGGTCTTAA
CAGACGAAGGAGTCCATTCT

Downstream 100 bases:

>100_bases
CTAACCTCTGCGCCGCTGGGTTGGCACGGCTCACACAGCCAACTCAGCGGCTCCTCCACTGACCCACAGCAAAAGGAATA
TGTCATGACAGCAACAGAAG

Product: solute-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MNVRRSAFTSLLLILISSVIAACGSESATTAPTTGTGGTTSSGGKVVALFLPDAKTARYETADRPYFEAKMKELCPDCQV
IYNNANQDASLQLQQAEAALTNGAKVLVLDPVDSAAAASIADKAKAQNVPVIAYDRLILNSDGVSYYISFDNESVGKLQA
ESLVAQLDKQGIANPTIVMINGSPTDNNAKLFKAGAHSVFDPLVSAGKLTIANEYDTPDWSPDKAQDQMQQALTSMGNKV
DGVYAANDGTGGGAIAAMKAGGLSPLPPVTGQDAELAAIQRILAGDQYMTVYKAIKPQAEAAAELAFALLEGKTSDKATS
KVNNGKIDVPSILLTPIAVTKENVKDTIVKDQFHKVDQICAGDFAKACADAGIQ

Sequences:

>Translated_374_residues
MNVRRSAFTSLLLILISSVIAACGSESATTAPTTGTGGTTSSGGKVVALFLPDAKTARYETADRPYFEAKMKELCPDCQV
IYNNANQDASLQLQQAEAALTNGAKVLVLDPVDSAAAASIADKAKAQNVPVIAYDRLILNSDGVSYYISFDNESVGKLQA
ESLVAQLDKQGIANPTIVMINGSPTDNNAKLFKAGAHSVFDPLVSAGKLTIANEYDTPDWSPDKAQDQMQQALTSMGNKV
DGVYAANDGTGGGAIAAMKAGGLSPLPPVTGQDAELAAIQRILAGDQYMTVYKAIKPQAEAAAELAFALLEGKTSDKATS
KVNNGKIDVPSILLTPIAVTKENVKDTIVKDQFHKVDQICAGDFAKACADAGIQ
>Mature_374_residues
MNVRRSAFTSLLLILISSVIAACGSESATTAPTTGTGGTTSSGGKVVALFLPDAKTARYETADRPYFEAKMKELCPDCQV
IYNNANQDASLQLQQAEAALTNGAKVLVLDPVDSAAAASIADKAKAQNVPVIAYDRLILNSDGVSYYISFDNESVGKLQA
ESLVAQLDKQGIANPTIVMINGSPTDNNAKLFKAGAHSVFDPLVSAGKLTIANEYDTPDWSPDKAQDQMQQALTSMGNKV
DGVYAANDGTGGGAIAAMKAGGLSPLPPVTGQDAELAAIQRILAGDQYMTVYKAIKPQAEAAAELAFALLEGKTSDKATS
KVNNGKIDVPSILLTPIAVTKENVKDTIVKDQFHKVDQICAGDFAKACADAGIQ

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=309, Percent_Identity=43.3656957928803, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788473, Length=284, Percent_Identity=31.6901408450704, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39035; Mature: 39035

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRRSAFTSLLLILISSVIAACGSESATTAPTTGTGGTTSSGGKVVALFLPDAKTARYE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
TADRPYFEAKMKELCPDCQVIYNNANQDASLQLQQAEAALTNGAKVLVLDPVDSAAAASI
CCCCCHHHHHHHHHCCCCEEEECCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCHHHHHH
ADKAKAQNVPVIAYDRLILNSDGVSYYISFDNESVGKLQAESLVAQLDKQGIANPTIVMI
HHHHHHCCCCEEEECEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
NGSPTDNNAKLFKAGAHSVFDPLVSAGKLTIANEYDTPDWSPDKAQDQMQQALTSMGNKV
ECCCCCCCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
DGVYAANDGTGGGAIAAMKAGGLSPLPPVTGQDAELAAIQRILAGDQYMTVYKAIKPQAE
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHH
AAAELAFALLEGKTSDKATSKVNNGKIDVPSILLTPIAVTKENVKDTIVKDQFHKVDQIC
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AGDFAKACADAGIQ
HHHHHHHHHHCCCC
>Mature Secondary Structure
MNVRRSAFTSLLLILISSVIAACGSESATTAPTTGTGGTTSSGGKVVALFLPDAKTARYE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
TADRPYFEAKMKELCPDCQVIYNNANQDASLQLQQAEAALTNGAKVLVLDPVDSAAAASI
CCCCCHHHHHHHHHCCCCEEEECCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCHHHHHH
ADKAKAQNVPVIAYDRLILNSDGVSYYISFDNESVGKLQAESLVAQLDKQGIANPTIVMI
HHHHHHCCCCEEEECEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
NGSPTDNNAKLFKAGAHSVFDPLVSAGKLTIANEYDTPDWSPDKAQDQMQQALTSMGNKV
ECCCCCCCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
DGVYAANDGTGGGAIAAMKAGGLSPLPPVTGQDAELAAIQRILAGDQYMTVYKAIKPQAE
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHH
AAAELAFALLEGKTSDKATSKVNNGKIDVPSILLTPIAVTKENVKDTIVKDQFHKVDQIC
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AGDFAKACADAGIQ
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]