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The map label for this gene is miaA

Identifier: 159900778

GI number: 159900778

Start: 5440199

End: 5441146

Strand: Direct

Name: miaA

Synonym: Haur_4265

Alternate gene names: 159900778

Gene position: 5440199-5441146 (Clockwise)

Preceding gene: 159900775

Following gene: 159900780

Centisome position: 85.72

GC content: 53.06

Gene sequence:

>948_bases
ATGCAAGCACCACAACCATTAATCATCGCCATCGTTGGCCCAACTGCGGTTGGCAAAACTGCCTTTTCACTCGATTTAGC
CCAAGCCTTAAATGGCGAGATCGTCTCAGTCGATTCGCGCTTAGTCTATCGGGGGATGGATATTGGCACAGCCAAACCAA
CGCCTGCTGAACAGGCATTAGTCAAACATCATTTAATCGACGTGGTTAATCCTGATCAGGAATATAGCCTAGCGACCTAC
CAAGCGGCGGCCTACGCGGCGATTGCCCAAATTCAGCAGCAGGCCAAACAGCCAATTTTGGTGGGGGGCACAGGCCAATA
TATGGCAGCTCTACTTGAGGGTTGGAGCATTCCTGAAGTTGCGCCCAACTATGAATTACGAGCGCGGTACGAGCAACAGG
CGGCCAGCGAAGGTCATGCGGCGCTCCATCAACAACTGCAAACGATCGATCCCGAGGCCGCTAAAGCAATCGACCCAACT
AATGTGCGGCGGGTGATTCGCGCCTTAGAAGTTTTTCATGAAACAGGCCAGCCGATTAGCCAGCTTCAGCAGCGCAATCC
GCCACACTATCGCATATTAACGCTTGATCTAGAGCGACCGCGCGACGAACTGTATGCCCGCATCGATCAACGGGTTGATC
TGATGATGCGCGAAGGACTGATTGCCGAAGTTTGGGCTTTAATTCGCCAAGGCTATGGCTGGGAGTTACCATCGATGTCG
GGGTTGGGTTATGCCGAATTTCGGCCATTGTGGCAAGGCCAACAAAGTGCTGGCGCTTGCATCAGCCAACTCAAATTCAA
CACCCATCGTTTTGCTCGCAAACAAGGGGCGTGGTTTCGGCGTTTGCCCAAGCGGGTTAGCCTCGATGCCCGTCATACGG
ATTTACTGGCACAGGTGCAGGAGCTGCTTGCAATGCCTGAGCACGCACCAATACATACTGATCACTAG

Upstream 100 bases:

>100_bases
ACAGAATTATCCTGTAGTACTGTACCTCAAAAATAGCCAAAATACCGTCAGATCACAATTAACATTGCAACAATCGCTGG
CGGCTTGTATCATAGCGGCC

Downstream 100 bases:

>100_bases
CTTGTTGCAAAAACTGCTCAGGATCACCAGGCACGCTGTAGCCAAGCCGTCCAAACAGCGCATAAGCCAATTGAGTTGCA
ATCCGCAAGCGTCGATCGGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MQAPQPLIIAIVGPTAVGKTAFSLDLAQALNGEIVSVDSRLVYRGMDIGTAKPTPAEQALVKHHLIDVVNPDQEYSLATY
QAAAYAAIAQIQQQAKQPILVGGTGQYMAALLEGWSIPEVAPNYELRARYEQQAASEGHAALHQQLQTIDPEAAKAIDPT
NVRRVIRALEVFHETGQPISQLQQRNPPHYRILTLDLERPRDELYARIDQRVDLMMREGLIAEVWALIRQGYGWELPSMS
GLGYAEFRPLWQGQQSAGACISQLKFNTHRFARKQGAWFRRLPKRVSLDARHTDLLAQVQELLAMPEHAPIHTDH

Sequences:

>Translated_315_residues
MQAPQPLIIAIVGPTAVGKTAFSLDLAQALNGEIVSVDSRLVYRGMDIGTAKPTPAEQALVKHHLIDVVNPDQEYSLATY
QAAAYAAIAQIQQQAKQPILVGGTGQYMAALLEGWSIPEVAPNYELRARYEQQAASEGHAALHQQLQTIDPEAAKAIDPT
NVRRVIRALEVFHETGQPISQLQQRNPPHYRILTLDLERPRDELYARIDQRVDLMMREGLIAEVWALIRQGYGWELPSMS
GLGYAEFRPLWQGQQSAGACISQLKFNTHRFARKQGAWFRRLPKRVSLDARHTDLLAQVQELLAMPEHAPIHTDH
>Mature_315_residues
MQAPQPLIIAIVGPTAVGKTAFSLDLAQALNGEIVSVDSRLVYRGMDIGTAKPTPAEQALVKHHLIDVVNPDQEYSLATY
QAAAYAAIAQIQQQAKQPILVGGTGQYMAALLEGWSIPEVAPNYELRARYEQQAASEGHAALHQQLQTIDPEAAKAIDPT
NVRRVIRALEVFHETGQPISQLQQRNPPHYRILTLDLERPRDELYARIDQRVDLMMREGLIAEVWALIRQGYGWELPSMS
GLGYAEFRPLWQGQQSAGACISQLKFNTHRFARKQGAWFRRLPKRVSLDARHTDLLAQVQELLAMPEHAPIHTDH

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=321, Percent_Identity=31.7757009345794, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790613, Length=284, Percent_Identity=40.4929577464789, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=271, Percent_Identity=27.3062730627306, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=28.6689419795222, Blast_Score=126, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24649830, Length=334, Percent_Identity=32.6347305389222, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_HERA2 (A9AY15)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547025.1
- ProteinModelPortal:   A9AY15
- SMR:   A9AY15
- GeneID:   5736119
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4265
- HOGENOM:   HBG559459
- OMA:   QNSESPY
- ProtClustDB:   PRK00091
- BioCyc:   HAUR316274:HAUR_4265-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35195; Mature: 35195

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAPQPLIIAIVGPTAVGKTAFSLDLAQALNGEIVSVDSRLVYRGMDIGTAKPTPAEQAL
CCCCCCEEEEEECCCHHCHHHHHHHHHHHHCCCEEEECCHHHEECCCCCCCCCCCHHHHH
VKHHLIDVVNPDQEYSLATYQAAAYAAIAQIQQQAKQPILVGGTGQYMAALLEGWSIPEV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCCCC
APNYELRARYEQQAASEGHAALHQQLQTIDPEAAKAIDPTNVRRVIRALEVFHETGQPIS
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
QLQQRNPPHYRILTLDLERPRDELYARIDQRVDLMMREGLIAEVWALIRQGYGWELPSMS
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
GLGYAEFRPLWQGQQSAGACISQLKFNTHRFARKQGAWFRRLPKRVSLDARHTDLLAQVQ
CCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHCCCCHHHHHHHHHH
ELLAMPEHAPIHTDH
HHHCCCCCCCCCCCC
>Mature Secondary Structure
MQAPQPLIIAIVGPTAVGKTAFSLDLAQALNGEIVSVDSRLVYRGMDIGTAKPTPAEQAL
CCCCCCEEEEEECCCHHCHHHHHHHHHHHHCCCEEEECCHHHEECCCCCCCCCCCHHHHH
VKHHLIDVVNPDQEYSLATYQAAAYAAIAQIQQQAKQPILVGGTGQYMAALLEGWSIPEV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCCCC
APNYELRARYEQQAASEGHAALHQQLQTIDPEAAKAIDPTNVRRVIRALEVFHETGQPIS
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
QLQQRNPPHYRILTLDLERPRDELYARIDQRVDLMMREGLIAEVWALIRQGYGWELPSMS
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
GLGYAEFRPLWQGQQSAGACISQLKFNTHRFARKQGAWFRRLPKRVSLDARHTDLLAQVQ
CCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHCCCCHHHHHHHHHH
ELLAMPEHAPIHTDH
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA