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The map label for this gene is murI

Identifier: 159900727

GI number: 159900727

Start: 5369746

End: 5370510

Strand: Reverse

Name: murI

Synonym: Haur_4214

Alternate gene names: 159900727

Gene position: 5370510-5369746 (Counterclockwise)

Preceding gene: 159900728

Following gene: 159900726

Centisome position: 84.62

GC content: 52.03

Gene sequence:

>765_bases
ATGAAAGACCAACCAATTGGCATGTTCGATTCAGGGGTTGGTGGCTTATCGACCTTGCGTGATCTGCGGGCACTTTTGCC
GCACGAAGATATTATTTACTACGCCGATACTGGTAATTGTCCGTATGGCGGGCGTTCCCACGAAGAAATTGTGGCCTTAT
CTGAGCGCATCACCTATATTTTACTCGAACGCGGGGTTAAATTGATTGTGGTAGCCTGCAACACTGCGACGCTGCATGCC
GTTGATTATTTGCGCGAACATTTCAGTATCAGTTTCGTCGGCATGGAGCCAGGCATCAAGCCTGCGATTGCCCAAACCAA
AACTGGTGTGGTTGGTGTGATGGCGACCCAAGCCACCGTTGCAGGTGAACGATTTCAGCGCTTGATTGCCCGTTATGCAG
GCGATGTGCAGGTTGTGCCGCAAGCCTGTCCAGGTTTGGTCGAATTAATCGAGGCTGGCGAGTTACAAAGTGAAACAACT
CGCGATGCTGTCGCCCGTTATGTTGCACCATTGCTCAAGGCAGGTGCTGATACGATTGTGTTGGGCTGCACCCATTATCC
ATTTTTGCGCTCGTTGATTGCCGATGTTGCTGGGCCAAACGTCGCCTTGCTCGATACGGGTGCGGCGGTAGCCCGCCAAA
CCCAACGCTTGTTGGCAGCAGCCGATTTGCTCAACCCACAAACATCGCAAGGCAGCATCGAATGGCTCACCAGTGGCGAT
CCGGCCCATTTTGCTAAGATTCGGCAATGTTTAGAAATCGAGTAA

Upstream 100 bases:

>100_bases
ACCCCGAAGAAATGGTGCGCGGCGAGGATTTGCGCTGGCAGGCAATTTTCAAGGCCTTTGTTGAAGCTGCCAAAGCATGT
CGCAACGAGGCGATTCAGGC

Downstream 100 bases:

>100_bases
GCCTATGACGAGCTTAGATCTCAGGCTCACCCTTTCACGTAGCAGCGTGCCCGCAAACACTGGCGAACAAGTTTTGTATG
TTTTAGCTGAGGTTGTTGGT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKDQPIGMFDSGVGGLSTLRDLRALLPHEDIIYYADTGNCPYGGRSHEEIVALSERITYILLERGVKLIVVACNTATLHA
VDYLREHFSISFVGMEPGIKPAIAQTKTGVVGVMATQATVAGERFQRLIARYAGDVQVVPQACPGLVELIEAGELQSETT
RDAVARYVAPLLKAGADTIVLGCTHYPFLRSLIADVAGPNVALLDTGAAVARQTQRLLAAADLLNPQTSQGSIEWLTSGD
PAHFAKIRQCLEIE

Sequences:

>Translated_254_residues
MKDQPIGMFDSGVGGLSTLRDLRALLPHEDIIYYADTGNCPYGGRSHEEIVALSERITYILLERGVKLIVVACNTATLHA
VDYLREHFSISFVGMEPGIKPAIAQTKTGVVGVMATQATVAGERFQRLIARYAGDVQVVPQACPGLVELIEAGELQSETT
RDAVARYVAPLLKAGADTIVLGCTHYPFLRSLIADVAGPNVALLDTGAAVARQTQRLLAAADLLNPQTSQGSIEWLTSGD
PAHFAKIRQCLEIE
>Mature_254_residues
MKDQPIGMFDSGVGGLSTLRDLRALLPHEDIIYYADTGNCPYGGRSHEEIVALSERITYILLERGVKLIVVACNTATLHA
VDYLREHFSISFVGMEPGIKPAIAQTKTGVVGVMATQATVAGERFQRLIARYAGDVQVVPQACPGLVELIEAGELQSETT
RDAVARYVAPLLKAGADTIVLGCTHYPFLRSLIADVAGPNVALLDTGAAVARQTQRLLAAADLLNPQTSQGSIEWLTSGD
PAHFAKIRQCLEIE

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=214, Percent_Identity=42.0560747663551, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_HERA2 (A9AXC6)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546974.1
- ProteinModelPortal:   A9AXC6
- SMR:   A9AXC6
- GeneID:   5736926
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4214
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- BioCyc:   HAUR316274:HAUR_4214-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 27270; Mature: 27270

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDQPIGMFDSGVGGLSTLRDLRALLPHEDIIYYADTGNCPYGGRSHEEIVALSERITYI
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LLERGVKLIVVACNTATLHAVDYLREHFSISFVGMEPGIKPAIAQTKTGVVGVMATQATV
HHHCCCEEEEEEECCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHCCCCEEEEEHHHHHH
AGERFQRLIARYAGDVQVVPQACPGLVELIEAGELQSETTRDAVARYVAPLLKAGADTIV
HHHHHHHHHHHHCCCEEECCHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LGCTHYPFLRSLIADVAGPNVALLDTGAAVARQTQRLLAAADLLNPQTSQGSIEWLTSGD
EECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
PAHFAKIRQCLEIE
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKDQPIGMFDSGVGGLSTLRDLRALLPHEDIIYYADTGNCPYGGRSHEEIVALSERITYI
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LLERGVKLIVVACNTATLHAVDYLREHFSISFVGMEPGIKPAIAQTKTGVVGVMATQATV
HHHCCCEEEEEEECCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHCCCCEEEEEHHHHHH
AGERFQRLIARYAGDVQVVPQACPGLVELIEAGELQSETTRDAVARYVAPLLKAGADTIV
HHHHHHHHHHHHCCCEEECCHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LGCTHYPFLRSLIADVAGPNVALLDTGAAVARQTQRLLAAADLLNPQTSQGSIEWLTSGD
EECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
PAHFAKIRQCLEIE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA