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The map label for this gene is argF [H]

Identifier: 159900650

GI number: 159900650

Start: 5284319

End: 5285287

Strand: Reverse

Name: argF [H]

Synonym: Haur_4137

Alternate gene names: 159900650

Gene position: 5285287-5284319 (Counterclockwise)

Preceding gene: 159900651

Following gene: 159900649

Centisome position: 83.28

GC content: 53.04

Gene sequence:

>969_bases
ATGACACACCCACTTCTTGGTCGCGATTTACTGGCCTTGGCGGATTGGAACGACACTGAATTATTCGACGTGCTGGAAAC
TGCCGCCGAACTCAAACTACGCCAACAACGCCGCAAAGCTCACCCTCTTTTGGCCAACCGCACCTTGGCGATGATTTTCG
AAAAGCCCTCAACCCGCACCCGCGTTGGCTTTGAATCGGCGATGGTTCAACTCGGCGGGCATGGCATCTACCTCAGCCCA
CGTGATACCCAAATTGGCCGCGGTGAGACGATTGGCGACACTGCTCGGGTACTGAGCCGCATGTGCGATGCGATTATGGC
ACGGGTCAATGATCATGCAACGTTGGTCGAGTTGGCTCAGGCAGCCGATGTGCCAGTGATCAACGGGCTTTCCGATTGGC
TGCATCCAGTGCAAGCACTTGCCGATGCCCTGACCATTCGCGAGCATTTTGGCAGCTGCCAAGGCGTAAAATTAGTCTAT
CTCGGCGATCCCAATAATGTTTCCAATTCGTTGCTTGAGCTTGCCCCACGCTTGGGTATGGATTTTGCGATCGGCACGCC
ATTTGTGGCCAATGTTGATCAGCATTTGTTGGCCCAAGCTCGCCTGCAAGCCGCTGCCAATGGCACCAAAATCATTATCA
CCAATGATCCCTTGGAAGCCGTAAAAGATGCCGATGTGTTGTATACCGATGTCTGGGTCAGTATGGGTCAAGTCGTGGCC
GAAGGTCATTTGGAAGCCTTACAACCATTTCAAATTAATGCTGAGCTTTTAGCCGCAGCGCGGGCTGGAGCCAAAGTTAT
GCACTGTTTGCCAATGCATCGCGGCGAAGAAATTAGCGCTGATATTGCCGATGGCGAACGTTCGATTGTGTTTGATCAAG
CTGAAAATCGCTTGCATACCCACAAAGCCGTTTTGGTTGCAACCATCGCTGGCTTAGCCTTACGCCGCACCAAGCGCCTA
GCGGCCTAA

Upstream 100 bases:

>100_bases
CCGTGCGCGACCAACTGATTGCTGCTCAACACGTTTTAGCAGAAGGTTGAGGTGGCTGCTAGCACCCTCGCCCTGCCGTT
AATCAAACGGAGGCTTCCCA

Downstream 100 bases:

>100_bases
GCTGAGGGATCGGTTGTCAGGGGTTAGGGTTCAGGCGGACGGAACCACGAAGGATATGAAAGGGAGAGATGAGGATCGGT
TATTGGGGGACGGGGATCGG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTHPLLGRDLLALADWNDTELFDVLETAAELKLRQQRRKAHPLLANRTLAMIFEKPSTRTRVGFESAMVQLGGHGIYLSP
RDTQIGRGETIGDTARVLSRMCDAIMARVNDHATLVELAQAADVPVINGLSDWLHPVQALADALTIREHFGSCQGVKLVY
LGDPNNVSNSLLELAPRLGMDFAIGTPFVANVDQHLLAQARLQAAANGTKIIITNDPLEAVKDADVLYTDVWVSMGQVVA
EGHLEALQPFQINAELLAAARAGAKVMHCLPMHRGEEISADIADGERSIVFDQAENRLHTHKAVLVATIAGLALRRTKRL
AA

Sequences:

>Translated_322_residues
MTHPLLGRDLLALADWNDTELFDVLETAAELKLRQQRRKAHPLLANRTLAMIFEKPSTRTRVGFESAMVQLGGHGIYLSP
RDTQIGRGETIGDTARVLSRMCDAIMARVNDHATLVELAQAADVPVINGLSDWLHPVQALADALTIREHFGSCQGVKLVY
LGDPNNVSNSLLELAPRLGMDFAIGTPFVANVDQHLLAQARLQAAANGTKIIITNDPLEAVKDADVLYTDVWVSMGQVVA
EGHLEALQPFQINAELLAAARAGAKVMHCLPMHRGEEISADIADGERSIVFDQAENRLHTHKAVLVATIAGLALRRTKRL
AA
>Mature_321_residues
THPLLGRDLLALADWNDTELFDVLETAAELKLRQQRRKAHPLLANRTLAMIFEKPSTRTRVGFESAMVQLGGHGIYLSPR
DTQIGRGETIGDTARVLSRMCDAIMARVNDHATLVELAQAADVPVINGLSDWLHPVQALADALTIREHFGSCQGVKLVYL
GDPNNVSNSLLELAPRLGMDFAIGTPFVANVDQHLLAQARLQAAANGTKIIITNDPLEAVKDADVLYTDVWVSMGQVVAE
GHLEALQPFQINAELLAAARAGAKVMHCLPMHRGEEISADIADGERSIVFDQAENRLHTHKAVLVATIAGLALRRTKRLA
A

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=308, Percent_Identity=42.2077922077922, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=27.3885350318471, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=39.1437308868502, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=39.0909090909091, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=30.1282051282051, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI48994908, Length=319, Percent_Identity=28.2131661442006, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=28.0373831775701, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=308, Percent_Identity=37.3376623376623, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=26.797385620915, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=28.8888888888889, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35027; Mature: 34896

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPLLGRDLLALADWNDTELFDVLETAAELKLRQQRRKAHPLLANRTLAMIFEKPSTRT
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCH
RVGFESAMVQLGGHGIYLSPRDTQIGRGETIGDTARVLSRMCDAIMARVNDHATLVELAQ
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AADVPVINGLSDWLHPVQALADALTIREHFGSCQGVKLVYLGDPNNVSNSLLELAPRLGM
HCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
DFAIGTPFVANVDQHLLAQARLQAAANGTKIIITNDPLEAVKDADVLYTDVWVSMGQVVA
CEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHH
EGHLEALQPFQINAELLAAARAGAKVMHCLPMHRGEEISADIADGERSIVFDQAENRLHT
HCHHHHCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHH
HKAVLVATIAGLALRRTKRLAA
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THPLLGRDLLALADWNDTELFDVLETAAELKLRQQRRKAHPLLANRTLAMIFEKPSTRT
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCH
RVGFESAMVQLGGHGIYLSPRDTQIGRGETIGDTARVLSRMCDAIMARVNDHATLVELAQ
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AADVPVINGLSDWLHPVQALADALTIREHFGSCQGVKLVYLGDPNNVSNSLLELAPRLGM
HCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
DFAIGTPFVANVDQHLLAQARLQAAANGTKIIITNDPLEAVKDADVLYTDVWVSMGQVVA
CEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHH
EGHLEALQPFQINAELLAAARAGAKVMHCLPMHRGEEISADIADGERSIVFDQAENRLHT
HCHHHHCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHH
HKAVLVATIAGLALRRTKRLAA
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]