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The map label for this gene is glnA [H]

Identifier: 159900545

GI number: 159900545

Start: 5146802

End: 5148136

Strand: Reverse

Name: glnA [H]

Synonym: Haur_4032

Alternate gene names: 159900545

Gene position: 5148136-5146802 (Counterclockwise)

Preceding gene: 159900550

Following gene: 159900544

Centisome position: 81.12

GC content: 49.44

Gene sequence:

>1335_bases
ATGCCAGTCACAAAAGAAGAAATTTTAGCAAAAATTGTCGAACAAAAAGTCCAGTTTATTAATCTCCAATTTACCGATAT
TGTGGGGATTATTAAAAATGTGACGATTCCTGTTTCGCAAATTCACGAAGCACTCGACCATGGTGTATGGTTCGATGGCT
CATCGATTGAAGGTTTTGCCCGCATCGCCGAAAGCGATATGTATTTGGTGCCCGACCTCAATACCTATGCGGTGATTCCA
TGGGAGAGCACCGGCGATTATATCACCGCGCGGTTTATTTGCGATGTCTACACGCCTGATGATCAACCATTTGCTGGCGA
CCCGCGCCATGTGCTCAAACGCACGCTGGCCCAAGCCGCCGAATTGGGCTTTATCTATAATACTGGGCCTGAATTAGAAT
TTTTCTTGTTTAAACCAGGCCCGAACGGCGAGTTGATTCCGTTGCCCCACGATGCAGCGGGCTATTTTGATGTTTCAACT
GATTATGCTACCCATATTCGCCGCCAAATGGTAGCTTCATTGCAAACCTTCGGGATCAAAGTCGAAGCGAGCCATCATGA
AGTAGCGATTGGCCAGCATGAAATTGATTTTGAATATGGCGATGCCTTGACCACCGCCGATCATACCGTCACCTTTCGCA
CGGTACTGAAGGCAATTGCCCAATTAAATGGCTTGCATGCCACTTTTATGCCCAAGCCAATTGCTGGGATCAATGGGTCG
GGTATGCATGTTCATCAAAGTTTGTCATCGATCGTAACGGGTCGCAATGAGTTTCATGATCACGAAACCAACAATTTATC
GTTGTTGGCGCAGCATTTTACCGCAGGCTTGCTTGACCATGCCTTGGGCATGACCGCCGTGCTGGCTCCGCTGGTCAACT
CCTATAAACGGCTTGTGCCTGGCTATGAAGCACCAATTTATGTTAGTTGGGGCCGTACCAACCGATCAGCGCTCGTGCGC
ATTCCGCGCATCAGTGCTGGCCGCTCGAACCAAGCCACGCGGATTGAGTTGCGCTGCCCCGACCCATCGGCCAACCCCTA
TTTGGCCTTCTCGGTGATGTTGGCTGCTGGCCTTGATGGGATTAAGCGTAAACTCATGCCACCACCCTCAGCCGAAGAAG
ATTTGTACCACGTTGACCCAATTGCCCGTGGCCTGAAAACCTTGCCTGTTTCCTTGGGCGAAGCTTTGGAAGAATTAAAA
CGTGATAGTGTGATCCAAGAAGCTTTGGGTGAACACATTTACGAGCGCTATATCGAGGCCAAAACCCAAGAATGGGATGC
CTACCGCCGCCACGTCTCGCAATGGGAGCTTGATCGCTATTTGCCAATTTATTAG

Upstream 100 bases:

>100_bases
AGTACGAGCAAGTATTTTTGGCTATTTTGCCCTATAATGAGCAAACTCAACTGGGCTATTATGCACAGTGGCTAGGTGTC
AGCGAAGGAGCATTCAGGTT

Downstream 100 bases:

>100_bases
CAATTGGGGATCAGTTGTTGGGGATCGGGGATCGGAGTAAAGCCACTAAGGATAATGAGGCTATCGGCTATGGGATGTAG
GGATCATTGAAATCCTACGA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPVTKEEILAKIVEQKVQFINLQFTDIVGIIKNVTIPVSQIHEALDHGVWFDGSSIEGFARIAESDMYLVPDLNTYAVIP
WESTGDYITARFICDVYTPDDQPFAGDPRHVLKRTLAQAAELGFIYNTGPELEFFLFKPGPNGELIPLPHDAAGYFDVST
DYATHIRRQMVASLQTFGIKVEASHHEVAIGQHEIDFEYGDALTTADHTVTFRTVLKAIAQLNGLHATFMPKPIAGINGS
GMHVHQSLSSIVTGRNEFHDHETNNLSLLAQHFTAGLLDHALGMTAVLAPLVNSYKRLVPGYEAPIYVSWGRTNRSALVR
IPRISAGRSNQATRIELRCPDPSANPYLAFSVMLAAGLDGIKRKLMPPPSAEEDLYHVDPIARGLKTLPVSLGEALEELK
RDSVIQEALGEHIYERYIEAKTQEWDAYRRHVSQWELDRYLPIY

Sequences:

>Translated_444_residues
MPVTKEEILAKIVEQKVQFINLQFTDIVGIIKNVTIPVSQIHEALDHGVWFDGSSIEGFARIAESDMYLVPDLNTYAVIP
WESTGDYITARFICDVYTPDDQPFAGDPRHVLKRTLAQAAELGFIYNTGPELEFFLFKPGPNGELIPLPHDAAGYFDVST
DYATHIRRQMVASLQTFGIKVEASHHEVAIGQHEIDFEYGDALTTADHTVTFRTVLKAIAQLNGLHATFMPKPIAGINGS
GMHVHQSLSSIVTGRNEFHDHETNNLSLLAQHFTAGLLDHALGMTAVLAPLVNSYKRLVPGYEAPIYVSWGRTNRSALVR
IPRISAGRSNQATRIELRCPDPSANPYLAFSVMLAAGLDGIKRKLMPPPSAEEDLYHVDPIARGLKTLPVSLGEALEELK
RDSVIQEALGEHIYERYIEAKTQEWDAYRRHVSQWELDRYLPIY
>Mature_443_residues
PVTKEEILAKIVEQKVQFINLQFTDIVGIIKNVTIPVSQIHEALDHGVWFDGSSIEGFARIAESDMYLVPDLNTYAVIPW
ESTGDYITARFICDVYTPDDQPFAGDPRHVLKRTLAQAAELGFIYNTGPELEFFLFKPGPNGELIPLPHDAAGYFDVSTD
YATHIRRQMVASLQTFGIKVEASHHEVAIGQHEIDFEYGDALTTADHTVTFRTVLKAIAQLNGLHATFMPKPIAGINGSG
MHVHQSLSSIVTGRNEFHDHETNNLSLLAQHFTAGLLDHALGMTAVLAPLVNSYKRLVPGYEAPIYVSWGRTNRSALVRI
PRISAGRSNQATRIELRCPDPSANPYLAFSVMLAAGLDGIKRKLMPPPSAEEDLYHVDPIARGLKTLPVSLGEALEELKR
DSVIQEALGEHIYERYIEAKTQEWDAYRRHVSQWELDRYLPIY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=441, Percent_Identity=27.6643990929705, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1790301, Length=474, Percent_Identity=38.3966244725738, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=28.8248337028825, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49582; Mature: 49451

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTKEEILAKIVEQKVQFINLQFTDIVGIIKNVTIPVSQIHEALDHGVWFDGSSIEGFA
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCHHHH
RIAESDMYLVPDLNTYAVIPWESTGDYITARFICDVYTPDDQPFAGDPRHVLKRTLAQAA
HHHCCCEEEECCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
ELGFIYNTGPELEFFLFKPGPNGELIPLPHDAAGYFDVSTDYATHIRRQMVASLQTFGIK
HCCEEECCCCCEEEEEECCCCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHCEE
VEASHHEVAIGQHEIDFEYGDALTTADHTVTFRTVLKAIAQLNGLHATFMPKPIAGINGS
EECCCCEEEECCEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
GMHVHQSLSSIVTGRNEFHDHETNNLSLLAQHFTAGLLDHALGMTAVLAPLVNSYKRLVP
CHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYEAPIYVSWGRTNRSALVRIPRISAGRSNQATRIELRCPDPSANPYLAFSVMLAAGLDG
CCCCCEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCHHH
IKRKLMPPPSAEEDLYHVDPIARGLKTLPVSLGEALEELKRDSVIQEALGEHIYERYIEA
HHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTQEWDAYRRHVSQWELDRYLPIY
HCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PVTKEEILAKIVEQKVQFINLQFTDIVGIIKNVTIPVSQIHEALDHGVWFDGSSIEGFA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCHHHH
RIAESDMYLVPDLNTYAVIPWESTGDYITARFICDVYTPDDQPFAGDPRHVLKRTLAQAA
HHHCCCEEEECCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
ELGFIYNTGPELEFFLFKPGPNGELIPLPHDAAGYFDVSTDYATHIRRQMVASLQTFGIK
HCCEEECCCCCEEEEEECCCCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHCEE
VEASHHEVAIGQHEIDFEYGDALTTADHTVTFRTVLKAIAQLNGLHATFMPKPIAGINGS
EECCCCEEEECCEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
GMHVHQSLSSIVTGRNEFHDHETNNLSLLAQHFTAGLLDHALGMTAVLAPLVNSYKRLVP
CHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYEAPIYVSWGRTNRSALVRIPRISAGRSNQATRIELRCPDPSANPYLAFSVMLAAGLDG
CCCCCEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCHHH
IKRKLMPPPSAEEDLYHVDPIARGLKTLPVSLGEALEELKRDSVIQEALGEHIYERYIEA
HHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTQEWDAYRRHVSQWELDRYLPIY
HCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]