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The map label for this gene is agaA [H]

Identifier: 159900407

GI number: 159900407

Start: 4884400

End: 4885563

Strand: Direct

Name: agaA [H]

Synonym: Haur_3892

Alternate gene names: 159900407

Gene position: 4884400-4885563 (Clockwise)

Preceding gene: 159900400

Following gene: 159900410

Centisome position: 76.96

GC content: 51.46

Gene sequence:

>1164_bases
ATGACGACTTCAGAACAGCCTCTCGCACCTACGCCCCCAATGGGTTGGAACTCGTGGAACATGTTTGGTAGTACGATTCA
TGAAGATTCAGTCCGTGCCACTGCTGACGTGTTGGTTAGCTCAGGCCTCAAGGATTGTGGTTATAACTATGTGGTGATTG
ATGATTGCTGGTCAACCAAAGTTGGCCGCGATGGCAACGGCGATTTGGTTGCCGACCCCGAAAAATTCCCCAGTGGCATC
AAAGCGCTAGCCGATTATGTGCATAGCCTTGGTTTGAAAATTGGCATCTACTCCGATGCGGCGCATCTGACTTGCGCTAG
TTATCCTGGCAGCTTTGGCTTCGAGGAGCAAGATGCCCAATTATGGGCTTCGTGGGGCATCGATTTCCTCAAATATGATT
TTTGTTTTGCGCCAACCGACCAAGCCACTGCGATCGACCGTTACACCCGCATGGGCGAGGCGTTGCGCAAAACGAAGCGC
CAATTTCTCTACTCGTTGTGTGAGTGGGGTGGCCGCAGCCCACAGCTCTGGGGTCGCTCGGTTGGCGGGCATATGTGGCG
GGTCACTGGCGATATTTTCGATAGCTGGGTTGATATTTGGGTTGCGCCACACAAATATTATGGGGTAGGCATTGATACAG
CGATTGATATTGCCGCCAATTTAGCCGAATACGCTGGCCCTGATGCCTGGAACGATTTGGATATGCTGGTGGTTGGATTG
AAGGGCAAGGGTCAAATCTCTGGCGGTGGCTTATCATTCATCGAATATCAAACCCATATGTCGTTGTGGACGATCGCCTG
CTCGCCCTTGATGATCGGCTGCGATATTCGCAATATGGATCGCGATACCACCAGTTTATTGACTAATCGCGAAGTTTTGG
CCGTCAACCAAGATAGTTTGGGGATTGCTGGGCGGCGCGTGAAACAAACCGGCACGTGCGAAATTTGGAAAAAACCGCTC
GCCGATGGCTCGTTGGCGGTTGCCTTGATTAATCGTGGCTCGATCGGCAGCGATTTAAGCTTGCGAGCCAGCGATATTGG
CCTGCTCGATACGCCGAAATCAGTACGAAATTTGTGGGCGCAAGCAGATATTGCCGAGTTTGGCGAGGCTTGGCAAACCC
GCATTCAACCCCATGAAACATTATTGCTCAAAATCAAAGCCTAA

Upstream 100 bases:

>100_bases
ACTCTAAAAATATTATCCATGAAAAGCTTTGACATTGACTAAGCTGGCAACCTACAATGATCAAATGGTAAGGAACGGCC
CTGACCACATGGAGAAATGT

Downstream 100 bases:

>100_bases
ATTCACCAAAAACGCCCATGCGATCGTTGTGATCGCATGGGCGTTTCTTCGTTAATCAGGTTTAGGCATCGTTTTGAATC
AAGCACATAAAGACTTTATA

Product: alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTTSEQPLAPTPPMGWNSWNMFGSTIHEDSVRATADVLVSSGLKDCGYNYVVIDDCWSTKVGRDGNGDLVADPEKFPSGI
KALADYVHSLGLKIGIYSDAAHLTCASYPGSFGFEEQDAQLWASWGIDFLKYDFCFAPTDQATAIDRYTRMGEALRKTKR
QFLYSLCEWGGRSPQLWGRSVGGHMWRVTGDIFDSWVDIWVAPHKYYGVGIDTAIDIAANLAEYAGPDAWNDLDMLVVGL
KGKGQISGGGLSFIEYQTHMSLWTIACSPLMIGCDIRNMDRDTTSLLTNREVLAVNQDSLGIAGRRVKQTGTCEIWKKPL
ADGSLAVALINRGSIGSDLSLRASDIGLLDTPKSVRNLWAQADIAEFGEAWQTRIQPHETLLLKIKA

Sequences:

>Translated_387_residues
MTTSEQPLAPTPPMGWNSWNMFGSTIHEDSVRATADVLVSSGLKDCGYNYVVIDDCWSTKVGRDGNGDLVADPEKFPSGI
KALADYVHSLGLKIGIYSDAAHLTCASYPGSFGFEEQDAQLWASWGIDFLKYDFCFAPTDQATAIDRYTRMGEALRKTKR
QFLYSLCEWGGRSPQLWGRSVGGHMWRVTGDIFDSWVDIWVAPHKYYGVGIDTAIDIAANLAEYAGPDAWNDLDMLVVGL
KGKGQISGGGLSFIEYQTHMSLWTIACSPLMIGCDIRNMDRDTTSLLTNREVLAVNQDSLGIAGRRVKQTGTCEIWKKPL
ADGSLAVALINRGSIGSDLSLRASDIGLLDTPKSVRNLWAQADIAEFGEAWQTRIQPHETLLLKIKA
>Mature_386_residues
TTSEQPLAPTPPMGWNSWNMFGSTIHEDSVRATADVLVSSGLKDCGYNYVVIDDCWSTKVGRDGNGDLVADPEKFPSGIK
ALADYVHSLGLKIGIYSDAAHLTCASYPGSFGFEEQDAQLWASWGIDFLKYDFCFAPTDQATAIDRYTRMGEALRKTKRQ
FLYSLCEWGGRSPQLWGRSVGGHMWRVTGDIFDSWVDIWVAPHKYYGVGIDTAIDIAANLAEYAGPDAWNDLDMLVVGLK
GKGQISGGGLSFIEYQTHMSLWTIACSPLMIGCDIRNMDRDTTSLLTNREVLAVNQDSLGIAGRRVKQTGTCEIWKKPLA
DGSLAVALINRGSIGSDLSLRASDIGLLDTPKSVRNLWAQADIAEFGEAWQTRIQPHETLLLKIKA

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=344, Percent_Identity=40.1162790697674, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI4557781, Length=373, Percent_Identity=36.7292225201072, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17562946, Length=403, Percent_Identity=33.4987593052109, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24583253, Length=404, Percent_Identity=36.3861386138614, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI19922382, Length=410, Percent_Identity=33.9024390243902, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24654142, Length=410, Percent_Identity=33.9024390243902, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 42533; Mature: 42402

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00512 ALPHA_GALACTOSIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSEQPLAPTPPMGWNSWNMFGSTIHEDSVRATADVLVSSGLKDCGYNYVVIDDCWSTK
CCCCCCCCCCCCCCCCCCHHHCCCHHCCCHHHHHHHHHHHCCHHHHCCCEEEEECCCCCC
VGRDGNGDLVADPEKFPSGIKALADYVHSLGLKIGIYSDAAHLTCASYPGSFGFEEQDAQ
CCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCHHHHH
LWASWGIDFLKYDFCFAPTDQATAIDRYTRMGEALRKTKRQFLYSLCEWGGRSPQLWGRS
HHHHHCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
VGGHMWRVTGDIFDSWVDIWVAPHKYYGVGIDTAIDIAANLAEYAGPDAWNDLDMLVVGL
CCCEEEEEEHHHHHHHHHHEECCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
KGKGQISGGGLSFIEYQTHMSLWTIACSPLMIGCDIRNMDRDTTSLLTNREVLAVNQDSL
CCCCEECCCCEEEEEEHHHHEEEEEECCCEEEECCCCCCCCHHHHHHCCCEEEEECCCCC
GIAGRRVKQTGTCEIWKKPLADGSLAVALINRGSIGSDLSLRASDIGLLDTPKSVRNLWA
CCCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHH
QADIAEFGEAWQTRIQPHETLLLKIKA
HCCHHHHHHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure 
TTSEQPLAPTPPMGWNSWNMFGSTIHEDSVRATADVLVSSGLKDCGYNYVVIDDCWSTK
CCCCCCCCCCCCCCCCCHHHCCCHHCCCHHHHHHHHHHHCCHHHHCCCEEEEECCCCCC
VGRDGNGDLVADPEKFPSGIKALADYVHSLGLKIGIYSDAAHLTCASYPGSFGFEEQDAQ
CCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCHHHHH
LWASWGIDFLKYDFCFAPTDQATAIDRYTRMGEALRKTKRQFLYSLCEWGGRSPQLWGRS
HHHHHCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
VGGHMWRVTGDIFDSWVDIWVAPHKYYGVGIDTAIDIAANLAEYAGPDAWNDLDMLVVGL
CCCEEEEEEHHHHHHHHHHEECCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
KGKGQISGGGLSFIEYQTHMSLWTIACSPLMIGCDIRNMDRDTTSLLTNREVLAVNQDSL
CCCCEECCCCEEEEEEHHHHEEEEEECCCEEEECCCCCCCCHHHHHHCCCEEEEECCCCC
GIAGRRVKQTGTCEIWKKPLADGSLAVALINRGSIGSDLSLRASDIGLLDTPKSVRNLWA
CCCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHH
QADIAEFGEAWQTRIQPHETLLLKIKA
HCCHHHHHHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA