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The map label for this gene is lacI [H]

Identifier: 159900182

GI number: 159900182

Start: 4608203

End: 4609234

Strand: Direct

Name: lacI [H]

Synonym: Haur_3665

Alternate gene names: 159900182

Gene position: 4608203-4609234 (Clockwise)

Preceding gene: 159900181

Following gene: 159900184

Centisome position: 72.61

GC content: 51.16

Gene sequence:

>1032_bases
ATGACCATTCAGCGTCCACAAAAAGTCACGATCAAAGATATTGCCAAACTGTGTAATGTTTCGACCCAAACCGTTTCGCG
GGTGCTCAACAATCGGCCTGATGTTTCGCCGCAAACCCGTGAGGCCGTTGAAAAAGCCATCGCTGATATGGGCTATCAGC
CAAGTGCCTTAGCCCGCAGCCTCGTGCAACAGCAAAGCTTTACCTTGGGGGTAATTACCGCAGGCTTGCAATATATGGGC
GTGTCGCTCACGTTGAATGGAATTGCCGAAGAAAGCGAAGCCTCGAATTATGCCCTGTTGCTCAAAGAATTACCGCGCTT
TGATCTGACCAACATTGTGCCAATTATTGAAGCGCTCATGGCCCGCCATGTTGATGGGATTATCTTTGCGGCGGCTGAAC
TCAACGAAAATGTCGAGCTGGTTCAAGCTCAACTTCCAGCAACCTGCCCACCGATTGTTTTTGTCAAAAGCCAACCTAAT
GAGCGCTTTACCACAATTGGCATCGATAATTATGGCGGTGCACGCCAAGCAGTTGAGCATCTGCTCAGCATTGGGCGGCG
CGAAATTGGCCTGATTTGCGGGCCAATCGAATGGCTCGAAACCCGCCAACGCCGTAGTGGCTGGTACGATGGATTAGCGG
CAGCAGGCATTACGCCAACCCAACAGCAAATTGGCATTGGCAATTGGTCGGCAGCCAGCGGCGAGCAAGCCTGCGCCGAA
TTATTCAAACGCTACCCACAGATGGATGCCGTATTTGCTTGTAACGATCAAATGGCCTTGGGTGCGTTGCATTATGCCAG
CAGTCATGGCATTCGCGTGCCCGAAGATTTGGCAGTTGTCGGCTTTGATGATTTAGCTGAGGCCGCCTATTTTTCACCAG
CACTGACCACGATTCGCCAACCATTGCGTGAATTAGGCCGCTTGGCCGTTCAAACCTTGTTGCAATTGATCAATCCTAAT
GAAGGCCAAATGCCACCAGTTGTAACAACCATGCCAACCCAATTGATCGTGCGCGACAGTAGCCTGAGCTAA

Upstream 100 bases:

>100_bases
AGCAGGGCAACTTGGCGTGAGCAACGCTGGGACAAAATCGGCTATACTACCGACTTGCGTCCCAGCGATCTGAAATGCAC
CATGCTATTGGGAGTTCGCC

Downstream 100 bases:

>100_bases
GCAATTTTACGGGTAACTAGCGCCAATTCCCAGACGATGTTGCTACGCTGGCTATAGTTTACAACATAATAATTACCTTC
AATTGCACGTTCGTAGTGAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTIQRPQKVTIKDIAKLCNVSTQTVSRVLNNRPDVSPQTREAVEKAIADMGYQPSALARSLVQQQSFTLGVITAGLQYMG
VSLTLNGIAEESEASNYALLLKELPRFDLTNIVPIIEALMARHVDGIIFAAAELNENVELVQAQLPATCPPIVFVKSQPN
ERFTTIGIDNYGGARQAVEHLLSIGRREIGLICGPIEWLETRQRRSGWYDGLAAAGITPTQQQIGIGNWSAASGEQACAE
LFKRYPQMDAVFACNDQMALGALHYASSHGIRVPEDLAVVGFDDLAEAAYFSPALTTIRQPLRELGRLAVQTLLQLINPN
EGQMPPVVTTMPTQLIVRDSSLS

Sequences:

>Translated_343_residues
MTIQRPQKVTIKDIAKLCNVSTQTVSRVLNNRPDVSPQTREAVEKAIADMGYQPSALARSLVQQQSFTLGVITAGLQYMG
VSLTLNGIAEESEASNYALLLKELPRFDLTNIVPIIEALMARHVDGIIFAAAELNENVELVQAQLPATCPPIVFVKSQPN
ERFTTIGIDNYGGARQAVEHLLSIGRREIGLICGPIEWLETRQRRSGWYDGLAAAGITPTQQQIGIGNWSAASGEQACAE
LFKRYPQMDAVFACNDQMALGALHYASSHGIRVPEDLAVVGFDDLAEAAYFSPALTTIRQPLRELGRLAVQTLLQLINPN
EGQMPPVVTTMPTQLIVRDSSLS
>Mature_342_residues
TIQRPQKVTIKDIAKLCNVSTQTVSRVLNNRPDVSPQTREAVEKAIADMGYQPSALARSLVQQQSFTLGVITAGLQYMGV
SLTLNGIAEESEASNYALLLKELPRFDLTNIVPIIEALMARHVDGIIFAAAELNENVELVQAQLPATCPPIVFVKSQPNE
RFTTIGIDNYGGARQAVEHLLSIGRREIGLICGPIEWLETRQRRSGWYDGLAAAGITPTQQQIGIGNWSAASGEQACAEL
FKRYPQMDAVFACNDQMALGALHYASSHGIRVPEDLAVVGFDDLAEAAYFSPALTTIRQPLRELGRLAVQTLLQLINPNE
GQMPPVVTTMPTQLIVRDSSLS

Specific function: Repressor of the lactose operon. Binds allolactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=32.5373134328358, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1788474, Length=311, Percent_Identity=34.0836012861736, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=33.1325301204819, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=32.0359281437126, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=30.7462686567164, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=31.7152103559871, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=29.940119760479, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787580, Length=340, Percent_Identity=28.2352941176471, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790715, Length=321, Percent_Identity=27.1028037383178, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=25.2336448598131, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=26.0606060606061, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787906, Length=339, Percent_Identity=24.7787610619469, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789456, Length=350, Percent_Identity=22.2857142857143, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37381; Mature: 37250

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQRPQKVTIKDIAKLCNVSTQTVSRVLNNRPDVSPQTREAVEKAIADMGYQPSALARS
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
LVQQQSFTLGVITAGLQYMGVSLTLNGIAEESEASNYALLLKELPRFDLTNIVPIIEALM
HHHHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
ARHVDGIIFAAAELNENVELVQAQLPATCPPIVFVKSQPNERFTTIGIDNYGGARQAVEH
HHCCCCCEEEHHHCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHH
LLSIGRREIGLICGPIEWLETRQRRSGWYDGLAAAGITPTQQQIGIGNWSAASGEQACAE
HHHCCCHHHCEEECCHHHHHHHHHHCCCCCCHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
LFKRYPQMDAVFACNDQMALGALHYASSHGIRVPEDLAVVGFDDLAEAAYFSPALTTIRQ
HHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHH
PLRELGRLAVQTLLQLINPNEGQMPPVVTTMPTQLIVRDSSLS
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCC
>Mature Secondary Structure 
TIQRPQKVTIKDIAKLCNVSTQTVSRVLNNRPDVSPQTREAVEKAIADMGYQPSALARS
CCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
LVQQQSFTLGVITAGLQYMGVSLTLNGIAEESEASNYALLLKELPRFDLTNIVPIIEALM
HHHHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
ARHVDGIIFAAAELNENVELVQAQLPATCPPIVFVKSQPNERFTTIGIDNYGGARQAVEH
HHCCCCCEEEHHHCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHH
LLSIGRREIGLICGPIEWLETRQRRSGWYDGLAAAGITPTQQQIGIGNWSAASGEQACAE
HHHCCCHHHCEEECCHHHHHHHHHHCCCCCCHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
LFKRYPQMDAVFACNDQMALGALHYASSHGIRVPEDLAVVGFDDLAEAAYFSPALTTIRQ
HHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHH
PLRELGRLAVQTLLQLINPNEGQMPPVVTTMPTQLIVRDSSLS
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 355891; 9278503; 1107032; 4571224; 4594037; 3286877; 7498473; 2178920; 8046748; 2040302; 1923807; 3064080; 2742823; 8683581; 10647179; 8638105 [H]