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The map label for this gene is asnB [H]

Identifier: 159900101

GI number: 159900101

Start: 4506549

End: 4508435

Strand: Direct

Name: asnB [H]

Synonym: Haur_3584

Alternate gene names: 159900101

Gene position: 4506549-4508435 (Clockwise)

Preceding gene: 159900100

Following gene: 159900102

Centisome position: 71.01

GC content: 51.51

Gene sequence:

>1887_bases
ATGTGTGGTATTTGTGGCATTGTCTATTTTGATCGCAGCCGTACAGCCGAGCGACCCTTGCTCGAAGCACTGTGCGACAA
TATCCATCATCGCGGGCCAGATCAAAATGGCTTTTATCTCAATGGCCCAGTTGGGCTTGGCTCAGTCCGGCTTTCGATTA
TCGACGTTGCAGGCGGCAAAATGCCCATCGCCAACGAAGATGGCTCGATCTGGATCGTCTATAACGGTGAGGTTTACAAC
TTTACCGAGTTGCGCCCACGCTTGGAAAAAGCTGGCCATAAATTTGAAACCCACTCCGATACTGAAACAATCGTGCATTT
GTACGAAGAAATTGGCGATGAATTTCCCAAGCATCTTAACGGCATGTTTGCCTGCGCAATTTGGGACGACCGTCGCCAGC
GCTTGGTGATTGCCCGCGATCACGTTGGGATCAAGCCAGTCTATTATGCCAATTTAGGCGATCGCTTGGTCTTTGGCTCG
GAAATCAAGGCCATGCTCGATACAGGCATCAGCCGCGAAATCGACCCAATTGGCTTGCACGATTATCTTTCGCTGAATTA
TGTGCCTGGCCCAAACACAATTTTCAAAGCGATCAAAAAGCTTCAGCCTGGCCATATGCTGACCTACGAAGCTGAAACCA
ATGCCGTCGAAGTTAAGCAATATTGGGATATTCCACGGGTGGTTTCGCCGCACTTCAAAATCACCGACGACGTTGAAACC
GACTTGCTGAATCTGTTGCGCACGGTTGTGCAGGATCAACTGATTAGCGATGTGCCCTTGGGCGCATTTCTCAGCGGCGG
GGTTGATTCGAGTTTGGTCGTGGCTTTGATGAGCGAAGTCACCAACCAACCAGTTAAAACCTTCTCGGTAGGCTTTGCTG
AAAAATCGTATGACGAATCGCCGTATGCGCGAATTGTCGCCGAACGCTTCCGCACCGATCACCACGAAATCATCATGAAT
CCTGATGCGCAGGCCACGGTTGATGCCATGGTTAAGTATTTTGACGAGCCATTCGCCGATTCGTCGTCGGTGGCGGTCTA
TGCAGTCAGCGAATTGGCGCGGCAGCATGTCAAAGTGGCGCTCAGTGGCGATGGTGGCGATGAGGTGTTTGGTGGCTATG
CCACCTATCAAGCAGACAAAATTGCCGCGCTCTATCGGCGTTTGCCCCAAGCGATTGGCGGCGGGTTAGTACCAAGTTTG
GTCAATATGCTGCCCACATCCGATGGCAAAGTCAGCTTCGATTTCAAGGCCAAGCGTTTTGTGCAAGGCGGGATGCTTGC
CCCGTTGCCAGCCCATGCCGCTTGGAAAGCCTTTATGAGCGAATCGATGAAAGAGCAGTTGTACGCTGGCTCGGCCATTT
TGACCAAGCAACGACCAACGGTTGATCTCTTGCAAGGCTATTACGATGCCTACAATGCCGACGATGTGCTCAATCGCTTG
CTGTATGTCGATTCCAAAGTGCAGTTGGCCGACGATATGCTGGTCAAAGTTGATCGCATGAGTATGGCCCACTCGCTCGA
AGTGCGCGTGCCGTTGCTCGATACGCGCTTGGTCGAGTGGATGGCCAAATTGCCCAGCCACACCAAAATCAATGGTATGA
AGCTCAAGTATCTGCTCAAGCGAGTGGCCGCCAAAGTGCTGCCAGCCCAAATTTTGGAGCGGCGCAAAGCTGGGTTTAGC
GTGCCAATTCCCCAATGGCTCAAAACCGATCTACGGCCATTGGTCAATCAAAAACTTTCGGTTGAGGCCTTGACCGAACA
AGGTTTCTTCAACCCACAATATATCGCAACAATGCTGAGTGATCACTGGGCAGGGCGGCACGATTACAGCCGCCAAATCT
GGAATTTACTGATGTTCAGCATGTGGTACGAGCGGTACGGCAAATAA

Upstream 100 bases:

>100_bases
CCTATATCGCTTTGTCCGACGACGACAAACCACGCTATGGCAGCGAGTGGGGCGGCGCACGAACTAACTTCGACTGTTGT
TGGTGAACTGAGGTGATCCT

Downstream 100 bases:

>100_bases
GCGACGTACCACCAACCCTGCCAGCGTAACAGTGCGTTACGCTGGCCTATTGTCTCGCAAGGAGTTCGCATGGTTCGTTT
AGTGCGCGTTTTTCTGCTTT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MCGICGIVYFDRSRTAERPLLEALCDNIHHRGPDQNGFYLNGPVGLGSVRLSIIDVAGGKMPIANEDGSIWIVYNGEVYN
FTELRPRLEKAGHKFETHSDTETIVHLYEEIGDEFPKHLNGMFACAIWDDRRQRLVIARDHVGIKPVYYANLGDRLVFGS
EIKAMLDTGISREIDPIGLHDYLSLNYVPGPNTIFKAIKKLQPGHMLTYEAETNAVEVKQYWDIPRVVSPHFKITDDVET
DLLNLLRTVVQDQLISDVPLGAFLSGGVDSSLVVALMSEVTNQPVKTFSVGFAEKSYDESPYARIVAERFRTDHHEIIMN
PDAQATVDAMVKYFDEPFADSSSVAVYAVSELARQHVKVALSGDGGDEVFGGYATYQADKIAALYRRLPQAIGGGLVPSL
VNMLPTSDGKVSFDFKAKRFVQGGMLAPLPAHAAWKAFMSESMKEQLYAGSAILTKQRPTVDLLQGYYDAYNADDVLNRL
LYVDSKVQLADDMLVKVDRMSMAHSLEVRVPLLDTRLVEWMAKLPSHTKINGMKLKYLLKRVAAKVLPAQILERRKAGFS
VPIPQWLKTDLRPLVNQKLSVEALTEQGFFNPQYIATMLSDHWAGRHDYSRQIWNLLMFSMWYERYGK

Sequences:

>Translated_628_residues
MCGICGIVYFDRSRTAERPLLEALCDNIHHRGPDQNGFYLNGPVGLGSVRLSIIDVAGGKMPIANEDGSIWIVYNGEVYN
FTELRPRLEKAGHKFETHSDTETIVHLYEEIGDEFPKHLNGMFACAIWDDRRQRLVIARDHVGIKPVYYANLGDRLVFGS
EIKAMLDTGISREIDPIGLHDYLSLNYVPGPNTIFKAIKKLQPGHMLTYEAETNAVEVKQYWDIPRVVSPHFKITDDVET
DLLNLLRTVVQDQLISDVPLGAFLSGGVDSSLVVALMSEVTNQPVKTFSVGFAEKSYDESPYARIVAERFRTDHHEIIMN
PDAQATVDAMVKYFDEPFADSSSVAVYAVSELARQHVKVALSGDGGDEVFGGYATYQADKIAALYRRLPQAIGGGLVPSL
VNMLPTSDGKVSFDFKAKRFVQGGMLAPLPAHAAWKAFMSESMKEQLYAGSAILTKQRPTVDLLQGYYDAYNADDVLNRL
LYVDSKVQLADDMLVKVDRMSMAHSLEVRVPLLDTRLVEWMAKLPSHTKINGMKLKYLLKRVAAKVLPAQILERRKAGFS
VPIPQWLKTDLRPLVNQKLSVEALTEQGFFNPQYIATMLSDHWAGRHDYSRQIWNLLMFSMWYERYGK
>Mature_628_residues
MCGICGIVYFDRSRTAERPLLEALCDNIHHRGPDQNGFYLNGPVGLGSVRLSIIDVAGGKMPIANEDGSIWIVYNGEVYN
FTELRPRLEKAGHKFETHSDTETIVHLYEEIGDEFPKHLNGMFACAIWDDRRQRLVIARDHVGIKPVYYANLGDRLVFGS
EIKAMLDTGISREIDPIGLHDYLSLNYVPGPNTIFKAIKKLQPGHMLTYEAETNAVEVKQYWDIPRVVSPHFKITDDVET
DLLNLLRTVVQDQLISDVPLGAFLSGGVDSSLVVALMSEVTNQPVKTFSVGFAEKSYDESPYARIVAERFRTDHHEIIMN
PDAQATVDAMVKYFDEPFADSSSVAVYAVSELARQHVKVALSGDGGDEVFGGYATYQADKIAALYRRLPQAIGGGLVPSL
VNMLPTSDGKVSFDFKAKRFVQGGMLAPLPAHAAWKAFMSESMKEQLYAGSAILTKQRPTVDLLQGYYDAYNADDVLNRL
LYVDSKVQLADDMLVKVDRMSMAHSLEVRVPLLDTRLVEWMAKLPSHTKINGMKLKYLLKRVAAKVLPAQILERRKAGFS
VPIPQWLKTDLRPLVNQKLSVEALTEQGFFNPQYIATMLSDHWAGRHDYSRQIWNLLMFSMWYERYGK

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=402, Percent_Identity=28.1094527363184, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI168229252, Length=402, Percent_Identity=28.1094527363184, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI168229250, Length=402, Percent_Identity=28.1094527363184, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI296010848, Length=375, Percent_Identity=28.2666666666667, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI296010852, Length=305, Percent_Identity=26.8852459016393, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI296010850, Length=305, Percent_Identity=26.8852459016393, Blast_Score=90, Evalue=7e-18,
Organism=Escherichia coli, GI1786889, Length=395, Percent_Identity=31.3924050632911, Blast_Score=149, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71993933, Length=393, Percent_Identity=31.0432569974555, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=393, Percent_Identity=31.0432569974555, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=342, Percent_Identity=30.4093567251462, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17560178, Length=395, Percent_Identity=28.3544303797468, Blast_Score=109, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=425, Percent_Identity=26.3529411764706, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=431, Percent_Identity=26.6821345707657, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45553209, Length=402, Percent_Identity=30.8457711442786, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70578; Mature: 70578

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGICGIVYFDRSRTAERPLLEALCDNIHHRGPDQNGFYLNGPVGLGSVRLSIIDVAGGK
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEEECCCC
MPIANEDGSIWIVYNGEVYNFTELRPRLEKAGHKFETHSDTETIVHLYEEIGDEFPKHLN
CCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMFACAIWDDRRQRLVIARDHVGIKPVYYANLGDRLVFGSEIKAMLDTGISREIDPIGLH
CEEEEEEEECCCCEEEEEECCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCC
DYLSLNYVPGPNTIFKAIKKLQPGHMLTYEAETNAVEVKQYWDIPRVVSPHFKITDDVET
HHEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCEEEHHHHCCCCCCCCCCCEECCCHHH
DLLNLLRTVVQDQLISDVPLGAFLSGGVDSSLVVALMSEVTNQPVKTFSVGFAEKSYDES
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHEECCCCCCCCCC
PYARIVAERFRTDHHEIIMNPDAQATVDAMVKYFDEPFADSSSVAVYAVSELARQHVKVA
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHEEEE
LSGDGGDEVFGGYATYQADKIAALYRRLPQAIGGGLVPSLVNMLPTSDGKVSFDFKAKRF
EECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECHHHHHH
VQGGMLAPLPAHAAWKAFMSESMKEQLYAGSAILTKQRPTVDLLQGYYDAYNADDVLNRL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHH
LYVDSKVQLADDMLVKVDRMSMAHSLEVRVPLLDTRLVEWMAKLPSHTKINGMKLKYLLK
HHCCCHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RVAAKVLPAQILERRKAGFSVPIPQWLKTDLRPLVNQKLSVEALTEQGFFNPQYIATMLS
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHH
DHWAGRHDYSRQIWNLLMFSMWYERYGK
HHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGICGIVYFDRSRTAERPLLEALCDNIHHRGPDQNGFYLNGPVGLGSVRLSIIDVAGGK
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEEECCCC
MPIANEDGSIWIVYNGEVYNFTELRPRLEKAGHKFETHSDTETIVHLYEEIGDEFPKHLN
CCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMFACAIWDDRRQRLVIARDHVGIKPVYYANLGDRLVFGSEIKAMLDTGISREIDPIGLH
CEEEEEEEECCCCEEEEEECCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCC
DYLSLNYVPGPNTIFKAIKKLQPGHMLTYEAETNAVEVKQYWDIPRVVSPHFKITDDVET
HHEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCEEEHHHHCCCCCCCCCCCEECCCHHH
DLLNLLRTVVQDQLISDVPLGAFLSGGVDSSLVVALMSEVTNQPVKTFSVGFAEKSYDES
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHEECCCCCCCCCC
PYARIVAERFRTDHHEIIMNPDAQATVDAMVKYFDEPFADSSSVAVYAVSELARQHVKVA
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHEEEE
LSGDGGDEVFGGYATYQADKIAALYRRLPQAIGGGLVPSLVNMLPTSDGKVSFDFKAKRF
EECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECHHHHHH
VQGGMLAPLPAHAAWKAFMSESMKEQLYAGSAILTKQRPTVDLLQGYYDAYNADDVLNRL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHH
LYVDSKVQLADDMLVKVDRMSMAHSLEVRVPLLDTRLVEWMAKLPSHTKINGMKLKYLLK
HHCCCHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RVAAKVLPAQILERRKAGFSVPIPQWLKTDLRPLVNQKLSVEALTEQGFFNPQYIATMLS
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHH
DHWAGRHDYSRQIWNLLMFSMWYERYGK
HHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]