Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 159900018

GI number: 159900018

Start: 4409247

End: 4411166

Strand: Reverse

Name: appA [H]

Synonym: Haur_3501

Alternate gene names: 159900018

Gene position: 4411166-4409247 (Counterclockwise)

Preceding gene: 159900019

Following gene: 159900017

Centisome position: 69.5

GC content: 49.69

Gene sequence:

>1920_bases
ATGCTTCGATCAAAGCGCCAATTGATGAGCTTTGCGCTCATGTTGGTCCTTGTTGTTCCGATCCTTGCTGCTTGTGGTGG
CGAAACAACTCCAACCACAGCTCCAGCAACGACTGCCCCAGCAACCGCAACTACAGATACCTCATCAGCAGCAACTGCTG
AACCAACTGCTGCTGAAGCAACCGTCGAACCAACCGTTGCTGAAGCAACCGCCGAACCAAGCACCGGTACTACTCCTGAT
ATGGATAAAACCATCATCATCGGTATGACCCAATCGCCAGATACCTTGTTCGGCATTGAATCACAATCAAGCGCCACGAC
CCAAGTGTTGTCAGCGATTCAACCAGCTTGTTTCACCACTTTGAGCTACGAATATCAACCAGTTTGTTTCACCAAATTGC
CAAGCTTCGAAGATGGCGATGCAGTGACCCAAACCGTCTCAGTTGATAGCGCCTATGCTGGCAACATCGTGATCGATGAT
GAGTTGATCACCGATACCGCTAGCTTGACCGAAGCAATCGAATTGGAACAAGTTGTTGTGACTTGGACCTTGATCGATGG
CATGACTTGGGAAGATGGTACGCCAATTACCGCTGCCGACTTCGTGTTTGCTGCTGAATTGTACCAAGATCCAGGCATCA
AGAACGCTAGCCGCTTCGTGCTTGATCGCACCGAAAAATACGAAGCCAAAGACGAAAAAACCTTGGTATGGTACGCTGCA
CCAGGTTACACCGATGCAACCTACTTCTTGAACACCTTTGGGCCTGAACCAAAGCACGTTTTGGAAGGCGAAGATCCAGC
AACCATTGGTGGTAGCGACTACGCTAGCAAGCCATTGGCATATGGCCCATACAAGATTGCTGAAAACACCCCACAAGAAA
GCACCAAGTTGGTTGCAAACGAAACCTACTGGAAAAAAGGTTTCCCTCTCGTTGGTAATGTTACCTTCAAGTATCTGACC
AGCGAAGATCAAGTGTTGCAACAATTGGAAAGCGGCGAAATCGACGTAGTTGGTTCAATTGGTTTGACCTTGGCTAACGC
TCCTAAGCTCGACGAACTCGAAGCTGCTGGCGTGCTCAAGGGCCAATATGTTCCAGCAACCGTGTGGGAACACATGGACT
TCGGTGTCGAGCGCAACGACGGCCAACCATCAGTATTCGCTGATGTCAAGTTGCGCCAAGCTGTTGCTTACGCTGTCAAC
CGCAAACAAATCATCGATAACGTCTTGTTCGGCAAGACCGTTGTGATGAACACCTTCTTGCCAGCCGACCACTGGGCTTA
TCCACCAAACGGCGAAGGCTTGGAAGCATACGAATATGATGTAGAAAAAGCTAAGGCTCTCTTGGCTGAAGCTGGTTGGG
TTGCTGGCGCTGATGGCATTCTTGAAAAAGATGGCACCAAGCTCACCATCCAATTCTACACCACCGAAAACAACCAAACC
CGCGAAGCCGTTGCTCAGTTGATCCAAGAAGACCTGAAAGCTGTTGGTATCGATGTTACCTTGAACTTCGTTCCAGCAAC
CGATGTCTTGTTCAAGAACGGCTCAGAAGGTATCTTGTCAGGCCGCCGCTTCGACTTAGGTTTGTACGCTTGGGTCAGTG
GCCCAGAGCCTTCGACCGCTCTGTACCTCTGTGAACAAGTGCCAACCGAAGAAAACAGCTTTGGTGGTCAAAACAACACT
GGCTGGTGTAACCCAGATTACGACAAGCCAGCCTTGGCCTCACAATCAGAAACCGACCGCGCCAAGCGGATTCCTTTGGT
CATCGAAGCTCAAAAAGTCTTCAATGCCGAATTGCCAACCTTCCCATTGTACCAACGTGTCAATGTTGGTGCCTACAACG
TCAAGGTTAGCGGCTTGGAATTGAACCCAACCAGCCAAGTTGACTTCTGGAACATCGAAACCTGGGATGTTACTGAGTAA

Upstream 100 bases:

>100_bases
TCGCGTCACCAACGAGACACCTGTTGGCCCTATATCCCTCTGATATAGGAATGTTCGATTTATAAATCGTCCTTTCAACT
GTTTGATTTAGGAGGCAAGA

Downstream 100 bases:

>100_bases
TCGGTAACCTTCCAGCTGCGGAGCTACGTGCCTACACGTGGCTCCGCAGTCGATCCCTCTCTTTCCCTACAGCTTTCGCG
AGCACCATGCAGCTAAATCA

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MLRSKRQLMSFALMLVLVVPILAACGGETTPTTAPATTAPATATTDTSSAATAEPTAAEATVEPTVAEATAEPSTGTTPD
MDKTIIIGMTQSPDTLFGIESQSSATTQVLSAIQPACFTTLSYEYQPVCFTKLPSFEDGDAVTQTVSVDSAYAGNIVIDD
ELITDTASLTEAIELEQVVVTWTLIDGMTWEDGTPITAADFVFAAELYQDPGIKNASRFVLDRTEKYEAKDEKTLVWYAA
PGYTDATYFLNTFGPEPKHVLEGEDPATIGGSDYASKPLAYGPYKIAENTPQESTKLVANETYWKKGFPLVGNVTFKYLT
SEDQVLQQLESGEIDVVGSIGLTLANAPKLDELEAAGVLKGQYVPATVWEHMDFGVERNDGQPSVFADVKLRQAVAYAVN
RKQIIDNVLFGKTVVMNTFLPADHWAYPPNGEGLEAYEYDVEKAKALLAEAGWVAGADGILEKDGTKLTIQFYTTENNQT
REAVAQLIQEDLKAVGIDVTLNFVPATDVLFKNGSEGILSGRRFDLGLYAWVSGPEPSTALYLCEQVPTEENSFGGQNNT
GWCNPDYDKPALASQSETDRAKRIPLVIEAQKVFNAELPTFPLYQRVNVGAYNVKVSGLELNPTSQVDFWNIETWDVTE

Sequences:

>Translated_639_residues
MLRSKRQLMSFALMLVLVVPILAACGGETTPTTAPATTAPATATTDTSSAATAEPTAAEATVEPTVAEATAEPSTGTTPD
MDKTIIIGMTQSPDTLFGIESQSSATTQVLSAIQPACFTTLSYEYQPVCFTKLPSFEDGDAVTQTVSVDSAYAGNIVIDD
ELITDTASLTEAIELEQVVVTWTLIDGMTWEDGTPITAADFVFAAELYQDPGIKNASRFVLDRTEKYEAKDEKTLVWYAA
PGYTDATYFLNTFGPEPKHVLEGEDPATIGGSDYASKPLAYGPYKIAENTPQESTKLVANETYWKKGFPLVGNVTFKYLT
SEDQVLQQLESGEIDVVGSIGLTLANAPKLDELEAAGVLKGQYVPATVWEHMDFGVERNDGQPSVFADVKLRQAVAYAVN
RKQIIDNVLFGKTVVMNTFLPADHWAYPPNGEGLEAYEYDVEKAKALLAEAGWVAGADGILEKDGTKLTIQFYTTENNQT
REAVAQLIQEDLKAVGIDVTLNFVPATDVLFKNGSEGILSGRRFDLGLYAWVSGPEPSTALYLCEQVPTEENSFGGQNNT
GWCNPDYDKPALASQSETDRAKRIPLVIEAQKVFNAELPTFPLYQRVNVGAYNVKVSGLELNPTSQVDFWNIETWDVTE
>Mature_639_residues
MLRSKRQLMSFALMLVLVVPILAACGGETTPTTAPATTAPATATTDTSSAATAEPTAAEATVEPTVAEATAEPSTGTTPD
MDKTIIIGMTQSPDTLFGIESQSSATTQVLSAIQPACFTTLSYEYQPVCFTKLPSFEDGDAVTQTVSVDSAYAGNIVIDD
ELITDTASLTEAIELEQVVVTWTLIDGMTWEDGTPITAADFVFAAELYQDPGIKNASRFVLDRTEKYEAKDEKTLVWYAA
PGYTDATYFLNTFGPEPKHVLEGEDPATIGGSDYASKPLAYGPYKIAENTPQESTKLVANETYWKKGFPLVGNVTFKYLT
SEDQVLQQLESGEIDVVGSIGLTLANAPKLDELEAAGVLKGQYVPATVWEHMDFGVERNDGQPSVFADVKLRQAVAYAVN
RKQIIDNVLFGKTVVMNTFLPADHWAYPPNGEGLEAYEYDVEKAKALLAEAGWVAGADGILEKDGTKLTIQFYTTENNQT
REAVAQLIQEDLKAVGIDVTLNFVPATDVLFKNGSEGILSGRRFDLGLYAWVSGPEPSTALYLCEQVPTEENSFGGQNNT
GWCNPDYDKPALASQSETDRAKRIPLVIEAQKVFNAELPTFPLYQRVNVGAYNVKVSGLELNPTSQVDFWNIETWDVTE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=229, Percent_Identity=30.5676855895196, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787551, Length=472, Percent_Identity=23.0932203389831, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789966, Length=360, Percent_Identity=23.0555555555556, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081878, Length=461, Percent_Identity=24.5119305856833, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787052, Length=511, Percent_Identity=22.1135029354207, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 69340; Mature: 69340

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSKRQLMSFALMLVLVVPILAACGGETTPTTAPATTAPATATTDTSSAATAEPTAAEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TVEPTVAEATAEPSTGTTPDMDKTIIIGMTQSPDTLFGIESQSSATTQVLSAIQPACFTT
CCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCHHHEE
LSYEYQPVCFTKLPSFEDGDAVTQTVSVDSAYAGNIVIDDELITDTASLTEAIELEQVVV
ECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCEEECCHHHCCHHHHHHHHHHCEEEE
TWTLIDGMTWEDGTPITAADFVFAAELYQDPGIKNASRFVLDRTEKYEAKDEKTLVWYAA
EEEEECCEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCHHCCCCCCCEEEEEEC
PGYTDATYFLNTFGPEPKHVLEGEDPATIGGSDYASKPLAYGPYKIAENTPQESTKLVAN
CCCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEECCCCCCCHHHEEEEC
ETYWKKGFPLVGNVTFKYLTSEDQVLQQLESGEIDVVGSIGLTLANAPKLDELEAAGVLK
CCHHHCCCCEEECEEEEEECCHHHHHHHHHCCCEEEEEECCEEECCCCCCCHHHHCCCCC
GQYVPATVWEHMDFGVERNDGQPSVFADVKLRQAVAYAVNRKQIIDNVLFGKTVVMNTFL
CCCCCHHHHHHHCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PADHWAYPPNGEGLEAYEYDVEKAKALLAEAGWVAGADGILEKDGTKLTIQFYTTENNQT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCCH
REAVAQLIQEDLKAVGIDVTLNFVPATDVLFKNGSEGILSGRRFDLGLYAWVSGPEPSTA
HHHHHHHHHHHHHHEEEEEEEEEECCCCEEEECCCCCCCCCCEEEEEEEEEECCCCCCCE
LYLCEQVPTEENSFGGQNNTGWCNPDYDKPALASQSETDRAKRIPLVIEAQKVFNAELPT
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEHHHHCCCCCC
FPLYQRVNVGAYNVKVSGLELNPTSQVDFWNIETWDVTE
CCCHHEECCCEEEEEEEEEEECCCCCEEEEECEEECCCC
>Mature Secondary Structure
MLRSKRQLMSFALMLVLVVPILAACGGETTPTTAPATTAPATATTDTSSAATAEPTAAEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TVEPTVAEATAEPSTGTTPDMDKTIIIGMTQSPDTLFGIESQSSATTQVLSAIQPACFTT
CCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCHHHEE
LSYEYQPVCFTKLPSFEDGDAVTQTVSVDSAYAGNIVIDDELITDTASLTEAIELEQVVV
ECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCEEECCHHHCCHHHHHHHHHHCEEEE
TWTLIDGMTWEDGTPITAADFVFAAELYQDPGIKNASRFVLDRTEKYEAKDEKTLVWYAA
EEEEECCEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCHHCCCCCCCEEEEEEC
PGYTDATYFLNTFGPEPKHVLEGEDPATIGGSDYASKPLAYGPYKIAENTPQESTKLVAN
CCCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEECCCCCCCHHHEEEEC
ETYWKKGFPLVGNVTFKYLTSEDQVLQQLESGEIDVVGSIGLTLANAPKLDELEAAGVLK
CCHHHCCCCEEECEEEEEECCHHHHHHHHHCCCEEEEEECCEEECCCCCCCHHHHCCCCC
GQYVPATVWEHMDFGVERNDGQPSVFADVKLRQAVAYAVNRKQIIDNVLFGKTVVMNTFL
CCCCCHHHHHHHCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PADHWAYPPNGEGLEAYEYDVEKAKALLAEAGWVAGADGILEKDGTKLTIQFYTTENNQT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEECCCCCH
REAVAQLIQEDLKAVGIDVTLNFVPATDVLFKNGSEGILSGRRFDLGLYAWVSGPEPSTA
HHHHHHHHHHHHHHEEEEEEEEEECCCCEEEECCCCCCCCCCEEEEEEEEEECCCCCCCE
LYLCEQVPTEENSFGGQNNTGWCNPDYDKPALASQSETDRAKRIPLVIEAQKVFNAELPT
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEHHHHCCCCCC
FPLYQRVNVGAYNVKVSGLELNPTSQVDFWNIETWDVTE
CCCHHEECCCEEEEEEEEEEECCCCCEEEEECEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]