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The map label for this gene is appA [H]

Identifier: 159900013

GI number: 159900013

Start: 4402834

End: 4404606

Strand: Reverse

Name: appA [H]

Synonym: Haur_3496

Alternate gene names: 159900013

Gene position: 4404606-4402834 (Counterclockwise)

Preceding gene: 159900014

Following gene: 159900012

Centisome position: 69.4

GC content: 50.31

Gene sequence:

>1773_bases
ATGCTTGACCTTGGATTGATGCTCATGAAATTGCGTTTTAGCCTTGCGCTGTTGCTCTGTTTTAGCTTGATTGGTTGTAG
TATTGAGGGTGGTGCACCAGCTGGTCAGCCCACTGTCCAAGTGCCAACTCCCTTGCCAACGACCAACCCTGCCACTTCCA
ATCTTTGGACGTTGGGCTTAACGGAGGAGCCAGTTGATCTGTATCCCTACAGTTATGCCTTTCGTGGCGCAGCTCCATTG
ATTGAATTGCTTTACCCTGCGCCTTTGACGGTGGTTGCTGAAGCCTATACCACAACTGGCGTTTTGGAGCGCGTGCCATC
ATTCGAAAATGGTGATGTGCAATATATTACAACCACGGTTAATCTTGATGCGAATGGCGTAATTACCACCACGCAAACCG
AAACGATTACCAGCGTGCGTCAGTTGACCGTCACCTATCGCTGGAATAAAAATTTGCAATGGGAAGATGGCCAACCATTA
ACCGCAGCTGATTCAGTTTTTGCCTATAATTTGTTGCGTGGTAGCGCCTCAACCGCCCAATTGGCAACCCAAAGCGACTT
AACCGCCGATTATGTGGCGATTGATCAATATACGACTCGTGCTTATTTGCCGCCCGAACGCGACGACCCAAATTATTTGG
TGACGGTCTGGACTCCATTGCCAGCGCATTTGTTTGAAGGTCAGCCAAGTGCCAAAGAAGTTAGCGATCGTTTGGCCCAG
TCGCCAGTTGGCTATGGCCCGTATACGCTCAAAGCCTGGACGGCAGGCACGCAGTTGGAATTTGTGCGACGCGAAGGTCA
AACGGAACAATTGCCTAGCACGATTATTGCCCGTTTATATCCTGATATTGCCATGATGCGCGACGATGTGCTGAGCGGGC
GGGTCGATGTGGCTTGGACAGAAGGTTCGTTGGAGCAATTAGCGCTTGATCTGAAAACTGATGTGCAATCCAAAACCTTG
CAATTGCTCCAAGCTCCCAACCCAATTTGGGAACATATCGACATGAATTTGGCGGTTGTAGCTTTGCAAGATATTCGGAT
GCGCCAAGCGATTGCCCATGGTTTTGACCGTGAGGCGATTAGCACAACCTTGTATGGCACGCCCAAAGCAGTTTTGCATA
GTTGGTTGGCGGCTGAATCATGGGCTTTTGATCCAACAACAGTGGTCAGTTATACCTTTGATCCTGCGCTTTCACGCCAA
TTGCTTGATGAAATGAATTATCGTGATACCAATGATGATGGTTTGCGCGAACGCCCTGATGGTACGCCCTTCCAATTGAC
ATTAACCACTTCGGCTCAAACCCCGATTCGCCAACGCCTGAGTGAGCAATTTGTCAGCGATATGCAGGCAATCGGGATTG
ATATTAAGGTTGAAGCCTTATCAACCACCGATTTGTATAGCCAGCAAGGGCCATTATTTGGGCGACGCTTTGAATTGGCC
TTGTTTGGCTGGCTGCGCAGCGTTGATCCCGATGGCGCGGTGTTGTGGAGTTGTGCCGCGATTCCTAACCAAATTAACGG
CTACTCCGGCGATAATTTTACTGGTTGGTGTATGGATACCGCCGATCGGGCGATTCGTACCGCGACCAGTTCGCTTGATC
CGGCGGTGCGTAAGGCTGCCTATAGCGAACAACAGCAAATTTTCACCCGCGAATTGCCAGTCTTGCCCGTGATTACCCGC
CAAACCACGGTGTTGCTTGCGCCGAATGTGCGAGGTGTGCAACCGCAACCATTGGCTCCAATTACTTGGAATGTGGGTGC
TTGGCAACGCTAG

Upstream 100 bases:

>100_bases
GCGGGCTGCGCTACACTGCCTGTTGCTTAAAAGTGATCGAACGTTGCGGTTTGAATAAATTAGAGCGTCAACCATCAGCA
CGTTGTTAATTGCTTGCTTG

Downstream 100 bases:

>100_bases
CGGCGCAGATTTTGCTTAATCGCGCTGATGACTACTGACGATCAACGTGGATCAAACTGAAATTATGCGCTTACTACTTG
GAATCTTATTGCTAAACTTA

Product: extracellular solute-binding protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MLDLGLMLMKLRFSLALLLCFSLIGCSIEGGAPAGQPTVQVPTPLPTTNPATSNLWTLGLTEEPVDLYPYSYAFRGAAPL
IELLYPAPLTVVAEAYTTTGVLERVPSFENGDVQYITTTVNLDANGVITTTQTETITSVRQLTVTYRWNKNLQWEDGQPL
TAADSVFAYNLLRGSASTAQLATQSDLTADYVAIDQYTTRAYLPPERDDPNYLVTVWTPLPAHLFEGQPSAKEVSDRLAQ
SPVGYGPYTLKAWTAGTQLEFVRREGQTEQLPSTIIARLYPDIAMMRDDVLSGRVDVAWTEGSLEQLALDLKTDVQSKTL
QLLQAPNPIWEHIDMNLAVVALQDIRMRQAIAHGFDREAISTTLYGTPKAVLHSWLAAESWAFDPTTVVSYTFDPALSRQ
LLDEMNYRDTNDDGLRERPDGTPFQLTLTTSAQTPIRQRLSEQFVSDMQAIGIDIKVEALSTTDLYSQQGPLFGRRFELA
LFGWLRSVDPDGAVLWSCAAIPNQINGYSGDNFTGWCMDTADRAIRTATSSLDPAVRKAAYSEQQQIFTRELPVLPVITR
QTTVLLAPNVRGVQPQPLAPITWNVGAWQR

Sequences:

>Translated_590_residues
MLDLGLMLMKLRFSLALLLCFSLIGCSIEGGAPAGQPTVQVPTPLPTTNPATSNLWTLGLTEEPVDLYPYSYAFRGAAPL
IELLYPAPLTVVAEAYTTTGVLERVPSFENGDVQYITTTVNLDANGVITTTQTETITSVRQLTVTYRWNKNLQWEDGQPL
TAADSVFAYNLLRGSASTAQLATQSDLTADYVAIDQYTTRAYLPPERDDPNYLVTVWTPLPAHLFEGQPSAKEVSDRLAQ
SPVGYGPYTLKAWTAGTQLEFVRREGQTEQLPSTIIARLYPDIAMMRDDVLSGRVDVAWTEGSLEQLALDLKTDVQSKTL
QLLQAPNPIWEHIDMNLAVVALQDIRMRQAIAHGFDREAISTTLYGTPKAVLHSWLAAESWAFDPTTVVSYTFDPALSRQ
LLDEMNYRDTNDDGLRERPDGTPFQLTLTTSAQTPIRQRLSEQFVSDMQAIGIDIKVEALSTTDLYSQQGPLFGRRFELA
LFGWLRSVDPDGAVLWSCAAIPNQINGYSGDNFTGWCMDTADRAIRTATSSLDPAVRKAAYSEQQQIFTRELPVLPVITR
QTTVLLAPNVRGVQPQPLAPITWNVGAWQR
>Mature_590_residues
MLDLGLMLMKLRFSLALLLCFSLIGCSIEGGAPAGQPTVQVPTPLPTTNPATSNLWTLGLTEEPVDLYPYSYAFRGAAPL
IELLYPAPLTVVAEAYTTTGVLERVPSFENGDVQYITTTVNLDANGVITTTQTETITSVRQLTVTYRWNKNLQWEDGQPL
TAADSVFAYNLLRGSASTAQLATQSDLTADYVAIDQYTTRAYLPPERDDPNYLVTVWTPLPAHLFEGQPSAKEVSDRLAQ
SPVGYGPYTLKAWTAGTQLEFVRREGQTEQLPSTIIARLYPDIAMMRDDVLSGRVDVAWTEGSLEQLALDLKTDVQSKTL
QLLQAPNPIWEHIDMNLAVVALQDIRMRQAIAHGFDREAISTTLYGTPKAVLHSWLAAESWAFDPTTVVSYTFDPALSRQ
LLDEMNYRDTNDDGLRERPDGTPFQLTLTTSAQTPIRQRLSEQFVSDMQAIGIDIKVEALSTTDLYSQQGPLFGRRFELA
LFGWLRSVDPDGAVLWSCAAIPNQINGYSGDNFTGWCMDTADRAIRTATSSLDPAVRKAAYSEQQQIFTRELPVLPVITR
QTTVLLAPNVRGVQPQPLAPITWNVGAWQR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=376, Percent_Identity=25.2659574468085, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789966, Length=464, Percent_Identity=20.4741379310345, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 65263; Mature: 65263

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLGLMLMKLRFSLALLLCFSLIGCSIEGGAPAGQPTVQVPTPLPTTNPATSNLWTLGL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECC
TEEPVDLYPYSYAFRGAAPLIELLYPAPLTVVAEAYTTTGVLERVPSFENGDVQYITTTV
CCCCCCCCCCCEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
NLDANGVITTTQTETITSVRQLTVTYRWNKNLQWEDGQPLTAADSVFAYNLLRGSASTAQ
EECCCCEEEECCHHHHHHEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
LATQSDLTADYVAIDQYTTRAYLPPERDDPNYLVTVWTPLPAHLFEGQPSAKEVSDRLAQ
HHHCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHH
SPVGYGPYTLKAWTAGTQLEFVRREGQTEQLPSTIIARLYPDIAMMRDDVLSGRVDVAWT
CCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEC
EGSLEQLALDLKTDVQSKTLQLLQAPNPIWEHIDMNLAVVALQDIRMRQAIAHGFDREAI
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCHHH
STTLYGTPKAVLHSWLAAESWAFDPTTVVSYTFDPALSRQLLDEMNYRDTNDDGLRERPD
HHEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCC
GTPFQLTLTTSAQTPIRQRLSEQFVSDMQAIGIDIKVEALSTTDLYSQQGPLFGRRFELA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCCCCCCCCHHHH
LFGWLRSVDPDGAVLWSCAAIPNQINGYSGDNFTGWCMDTADRAIRTATSSLDPAVRKAA
HHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHH
YSEQQQIFTRELPVLPVITRQTTVLLAPNVRGVQPQPLAPITWNVGAWQR
HHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MLDLGLMLMKLRFSLALLLCFSLIGCSIEGGAPAGQPTVQVPTPLPTTNPATSNLWTLGL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECC
TEEPVDLYPYSYAFRGAAPLIELLYPAPLTVVAEAYTTTGVLERVPSFENGDVQYITTTV
CCCCCCCCCCCEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
NLDANGVITTTQTETITSVRQLTVTYRWNKNLQWEDGQPLTAADSVFAYNLLRGSASTAQ
EECCCCEEEECCHHHHHHEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
LATQSDLTADYVAIDQYTTRAYLPPERDDPNYLVTVWTPLPAHLFEGQPSAKEVSDRLAQ
HHHCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHH
SPVGYGPYTLKAWTAGTQLEFVRREGQTEQLPSTIIARLYPDIAMMRDDVLSGRVDVAWT
CCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEC
EGSLEQLALDLKTDVQSKTLQLLQAPNPIWEHIDMNLAVVALQDIRMRQAIAHGFDREAI
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCHHH
STTLYGTPKAVLHSWLAAESWAFDPTTVVSYTFDPALSRQLLDEMNYRDTNDDGLRERPD
HHEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCC
GTPFQLTLTTSAQTPIRQRLSEQFVSDMQAIGIDIKVEALSTTDLYSQQGPLFGRRFELA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCCCCCCCCHHHH
LFGWLRSVDPDGAVLWSCAAIPNQINGYSGDNFTGWCMDTADRAIRTATSSLDPAVRKAA
HHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHH
YSEQQQIFTRELPVLPVITRQTTVLLAPNVRGVQPQPLAPITWNVGAWQR
HHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]