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The map label for this gene is sdhA [C]

Identifier: 159899970

GI number: 159899970

Start: 4341937

End: 4343667

Strand: Reverse

Name: sdhA [C]

Synonym: Haur_3453

Alternate gene names: 159899970

Gene position: 4343667-4341937 (Counterclockwise)

Preceding gene: 159899972

Following gene: 159899968

Centisome position: 68.44

GC content: 54.25

Gene sequence:

>1731_bases
ATGCGGCAGCGTTATACAACTGATGTTTTGGTGATTGGCACAGGTGGGGCAGGCTTACGCGCAGCGATCGAACTGCATGA
TCAAGGCTTGCAGGTCTTGTTGGTGGGCAAACGTCCTAAGACCGATGCGCATACGGTGTTGGCGGCTGGCGGCATCAACG
CAGCCCTAGCGACGGTCGATGTTGACGATACTTGGGCGATTCACGCCGCCGACACCTTGCAAGAAGGTCGCATGCTGGGC
GACCCGCGCATGGTCGAATTGCTCTGTCAACAAGCCCCCAAAGCGATTGCTGAACTTGAGGCTTGGGGCATGCCCTTTGC
CCGAACTAGTTCAGGATTGATCGATCAGCGCTATTTTGGGGCGCATCGCTATCGCCGCACCTGTTTTGTCGGTGATTACA
CTGGGCTTGAAATTATCAAAGCCTTGATCCGTGAAGTTGAGCGCCGCCAAATTCCGATTCTCGAACGCGCCTATATCAGC
GATTTGCTGCATACTGGCAATCGGGTCAATGGCGCATACGGCTTTTGGCTAGCCGATCAACGTGAGTTTGTGATCGAAAG
CAAAGCCGTGATTTTGGCGACGGGCGGCCATATTCATAGCTATCAGCGTTCCTCGTCGCGGCGGCGCGAAAATACTGGCG
ATGGTATGGCCTTGGGCTTGCAAGTCGGTGCATACTTGGCCGATATGGAGCTGGTGCAATTCCACCCATCGGGCATGATC
TTCCCGCATGAATTGGCTGGAACCTTGGTGACTGAAGCGGTGCGTGGCGAGGGCGGGCGACTGTACAATCGCCATGGTGA
ACGTTTTATGCAGCACTACGACCCTGAACGCATGGAATTATCGACCCGTGATCGGGTGGCTTTAGCCAATTACACCGAAA
TTATTGAAGGCCGTGGTACTGAGCATGGCGGGGTTTGGCTCGATATTAGTCATATTCCAGCAAGCTTAATCCACGAGCGC
TTGCCACGCATGGTTGAGCAATTTCAGGCGGTTGGGGTGGATATTACTCGCGAAGCCATGCAAATTGCGCCAACTGCCCA
TTATTCGATGGGTGGCTTGCAGGTCGATCCCTTCAGCCATGCCACCAATGTGGCTGGGTTATATGCGGCAGGCGAGGTTA
CGGCTGGCGTGCACGGGGCCAATCGGCTAGGTGGCAATAGTTTGGCCGAAATTCTGGTGTTTGGGCGGATTGCTGGCCAG
CATGCTGGCGAATATGTGCGCCAACACAGCGAACAAAGCCTGCATGAAACCCAAATGCAAGCCGCCGCTGAGCGTTTGGC
TTGGGCCAGCCAACAATCAGGAGCCGTCCAACGCAGCTTAATCGACCAATTGCAACAGCTGGTTTGGCATGGCGCTGGCG
TGGTACGCAACGAACAAACGCTCGAAGCCAGCCTTGATCAACTGCATACGCTGCGCTTTCAAGCTCAACAACACTCGGTT
GGCACAGCCGATATCGCTGCAACTTTAGATTTACAATCGATGTTATTGACTGCAGAAGCCACAATTTTAGCGGCCTTGAT
GCGCCCCGAAAGCCGTGGTGCGCATCAACGTAGCGATTACCCCGCGACTGATCCGCTCTGGCAGCGCACAATCGTGATTA
AGCCCTATCGTGATGAACACGAACGCTTGCGGCTGCAACTGGATACCCAATTAATTCCCACTGCCAGCGAGCAAGTGCAA
GCGGCCTTCAATTATCAAGAATATAGTATGGCTGGACGTTTGGTTGAATAG

Upstream 100 bases:

>100_bases
AACGGTAGAGTGTTTAACAACAACAAAATCGCCTTGGCGCAATACGGGGCAATAGTTGATTGATTGGGCCAAGGCTTCAC
GCTACACAACGAGGTATGCT

Downstream 100 bases:

>100_bases
TTGGGTTTGGGCCACAAAGGCAATGAAGTGAAGCTTTTGACTGTTGATTGGATTTTTTCCTCACCTCCTAACCCCCTCCT
TTCAAGGGTAGGTTTTCAAC

Product: succinate dehydrogenase

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MRQRYTTDVLVIGTGGAGLRAAIELHDQGLQVLLVGKRPKTDAHTVLAAGGINAALATVDVDDTWAIHAADTLQEGRMLG
DPRMVELLCQQAPKAIAELEAWGMPFARTSSGLIDQRYFGAHRYRRTCFVGDYTGLEIIKALIREVERRQIPILERAYIS
DLLHTGNRVNGAYGFWLADQREFVIESKAVILATGGHIHSYQRSSSRRRENTGDGMALGLQVGAYLADMELVQFHPSGMI
FPHELAGTLVTEAVRGEGGRLYNRHGERFMQHYDPERMELSTRDRVALANYTEIIEGRGTEHGGVWLDISHIPASLIHER
LPRMVEQFQAVGVDITREAMQIAPTAHYSMGGLQVDPFSHATNVAGLYAAGEVTAGVHGANRLGGNSLAEILVFGRIAGQ
HAGEYVRQHSEQSLHETQMQAAAERLAWASQQSGAVQRSLIDQLQQLVWHGAGVVRNEQTLEASLDQLHTLRFQAQQHSV
GTADIAATLDLQSMLLTAEATILAALMRPESRGAHQRSDYPATDPLWQRTIVIKPYRDEHERLRLQLDTQLIPTASEQVQ
AAFNYQEYSMAGRLVE

Sequences:

>Translated_576_residues
MRQRYTTDVLVIGTGGAGLRAAIELHDQGLQVLLVGKRPKTDAHTVLAAGGINAALATVDVDDTWAIHAADTLQEGRMLG
DPRMVELLCQQAPKAIAELEAWGMPFARTSSGLIDQRYFGAHRYRRTCFVGDYTGLEIIKALIREVERRQIPILERAYIS
DLLHTGNRVNGAYGFWLADQREFVIESKAVILATGGHIHSYQRSSSRRRENTGDGMALGLQVGAYLADMELVQFHPSGMI
FPHELAGTLVTEAVRGEGGRLYNRHGERFMQHYDPERMELSTRDRVALANYTEIIEGRGTEHGGVWLDISHIPASLIHER
LPRMVEQFQAVGVDITREAMQIAPTAHYSMGGLQVDPFSHATNVAGLYAAGEVTAGVHGANRLGGNSLAEILVFGRIAGQ
HAGEYVRQHSEQSLHETQMQAAAERLAWASQQSGAVQRSLIDQLQQLVWHGAGVVRNEQTLEASLDQLHTLRFQAQQHSV
GTADIAATLDLQSMLLTAEATILAALMRPESRGAHQRSDYPATDPLWQRTIVIKPYRDEHERLRLQLDTQLIPTASEQVQ
AAFNYQEYSMAGRLVE
>Mature_576_residues
MRQRYTTDVLVIGTGGAGLRAAIELHDQGLQVLLVGKRPKTDAHTVLAAGGINAALATVDVDDTWAIHAADTLQEGRMLG
DPRMVELLCQQAPKAIAELEAWGMPFARTSSGLIDQRYFGAHRYRRTCFVGDYTGLEIIKALIREVERRQIPILERAYIS
DLLHTGNRVNGAYGFWLADQREFVIESKAVILATGGHIHSYQRSSSRRRENTGDGMALGLQVGAYLADMELVQFHPSGMI
FPHELAGTLVTEAVRGEGGRLYNRHGERFMQHYDPERMELSTRDRVALANYTEIIEGRGTEHGGVWLDISHIPASLIHER
LPRMVEQFQAVGVDITREAMQIAPTAHYSMGGLQVDPFSHATNVAGLYAAGEVTAGVHGANRLGGNSLAEILVFGRIAGQ
HAGEYVRQHSEQSLHETQMQAAAERLAWASQQSGAVQRSLIDQLQQLVWHGAGVVRNEQTLEASLDQLHTLRFQAQQHSV
GTADIAATLDLQSMLLTAEATILAALMRPESRGAHQRSDYPATDPLWQRTIVIKPYRDEHERLRLQLDTQLIPTASEQVQ
AAFNYQEYSMAGRLVE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=531, Percent_Identity=35.4048964218456, Blast_Score=273, Evalue=4e-73,
Organism=Escherichia coli, GI1786942, Length=589, Percent_Identity=33.786078098472, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1790597, Length=520, Percent_Identity=34.6153846153846, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788928, Length=537, Percent_Identity=33.8919925512104, Blast_Score=240, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17550100, Length=530, Percent_Identity=35.4716981132075, Blast_Score=273, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17505833, Length=527, Percent_Identity=35.1043643263757, Blast_Score=272, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71986328, Length=69, Percent_Identity=55.0724637681159, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322701, Length=597, Percent_Identity=34.5058626465662, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6322416, Length=557, Percent_Identity=34.4703770197487, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322511, Length=477, Percent_Identity=26.6247379454927, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320788, Length=477, Percent_Identity=26.4150943396226, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17137288, Length=542, Percent_Identity=35.9778597785978, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24655642, Length=542, Percent_Identity=35.9778597785978, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24655647, Length=542, Percent_Identity=35.9778597785978, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24663005, Length=546, Percent_Identity=34.2490842490842, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 63719; Mature: 63719

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRYTTDVLVIGTGGAGLRAAIELHDQGLQVLLVGKRPKTDAHTVLAAGGINAALATVD
CCCCEECCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEE
VDDTWAIHAADTLQEGRMLGDPRMVELLCQQAPKAIAELEAWGMPFARTSSGLIDQRYFG
CCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AHRYRRTCFVGDYTGLEIIKALIREVERRQIPILERAYISDLLHTGNRVNGAYGFWLADQ
HHHHHCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
REFVIESKAVILATGGHIHSYQRSSSRRRENTGDGMALGLQVGAYLADMELVQFHPSGMI
CCEEEECCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCHHEEECCCCCC
FPHELAGTLVTEAVRGEGGRLYNRHGERFMQHYDPERMELSTRDRVALANYTEIIEGRGT
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHCCCC
EHGGVWLDISHIPASLIHERLPRMVEQFQAVGVDITREAMQIAPTAHYSMGGLQVDPFSH
CCCCEEEEHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCC
ATNVAGLYAAGEVTAGVHGANRLGGNSLAEILVFGRIAGQHAGEYVRQHSEQSLHETQMQ
CCCCCEEEECCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAERLAWASQQSGAVQRSLIDQLQQLVWHGAGVVRNEQTLEASLDQLHTLRFQAQQHSV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GTADIAATLDLQSMLLTAEATILAALMRPESRGAHQRSDYPATDPLWQRTIVIKPYRDEH
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
ERLRLQLDTQLIPTASEQVQAAFNYQEYSMAGRLVE
HHEEEEECCEECCCCHHHHHHHHCHHHHHHCCCCCC
>Mature Secondary Structure
MRQRYTTDVLVIGTGGAGLRAAIELHDQGLQVLLVGKRPKTDAHTVLAAGGINAALATVD
CCCCEECCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEE
VDDTWAIHAADTLQEGRMLGDPRMVELLCQQAPKAIAELEAWGMPFARTSSGLIDQRYFG
CCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AHRYRRTCFVGDYTGLEIIKALIREVERRQIPILERAYISDLLHTGNRVNGAYGFWLADQ
HHHHHCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
REFVIESKAVILATGGHIHSYQRSSSRRRENTGDGMALGLQVGAYLADMELVQFHPSGMI
CCEEEECCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCHHEEECCCCCC
FPHELAGTLVTEAVRGEGGRLYNRHGERFMQHYDPERMELSTRDRVALANYTEIIEGRGT
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHCCCC
EHGGVWLDISHIPASLIHERLPRMVEQFQAVGVDITREAMQIAPTAHYSMGGLQVDPFSH
CCCCEEEEHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCC
ATNVAGLYAAGEVTAGVHGANRLGGNSLAEILVFGRIAGQHAGEYVRQHSEQSLHETQMQ
CCCCCEEEECCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAERLAWASQQSGAVQRSLIDQLQQLVWHGAGVVRNEQTLEASLDQLHTLRFQAQQHSV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GTADIAATLDLQSMLLTAEATILAALMRPESRGAHQRSDYPATDPLWQRTIVIKPYRDEH
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
ERLRLQLDTQLIPTASEQVQAAFNYQEYSMAGRLVE
HHEEEEECCEECCCCHHHHHHHHCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.4 {fumarate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]