Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 159899369

GI number: 159899369

Start: 3617591

End: 3619405

Strand: Reverse

Name: thrS [H]

Synonym: Haur_2850

Alternate gene names: 159899369

Gene position: 3619405-3617591 (Counterclockwise)

Preceding gene: 159899370

Following gene: 159899365

Centisome position: 57.03

GC content: 50.91

Gene sequence:

>1815_bases
ATGCCACCAGTCAATCCAGATAATGATCCACTGTATCGCTTGCGCCACTCGACGGCGCACGTGTTGGCCCAAGCTGTGCT
CGAAATTTTCCCTGAGGGCAAGATTGCGATCGGCCCGCCGGTTGAAAATGGTTTTTACTACGATTTTGATCTGCCACGGC
CATTAACGCCCGATGATCTCAAAGATATCGAGGCCAAGATGCGCAAAATTATCAAGGCCAAACATCGCTTTGCTTATCGT
GAAGTTTCAGCAGATGAAGCCCGCGAATTGTTTAAGAATCAGCCCTACAAACTTGAGTTGATCGCAGGCTTGGCGAAGGG
CGAGGATGAATATGGTGAGAAAGCCGCTGCCAGCGATACGATCATCTCAACCTACAAACACGATTCGTTTGAAGATTTGT
GCAAAGGCCCACACGTCGAAAGTTTGGGCGATATTCCGCCGAATGGCTTTAAGTTGCTGCGAGTTTCGGGCGCATACTGG
CGTGGCGATGAAAAACGCCCAATGTTGCAACGGATTTATGGGACGGTCTGGCCATCAAAAGAGGAGCTTGATCATTATTT
GTGGCAGCAAGAAGAAGCTAAAAAGCGTGATCACCGCAAGCTTGGCCGCGAATTAGGCTTGTTCACCTTCTCGCAAAAAG
TTGGGGCTGGCTTGGCTTTGTGGTTGCCCAAAGGTGCAATTTTGCGCGATGTTTTGGAGCGATTTTTGCGCCAAGCTCAA
ATCGAACGCGGCTACTTGCCAGTCGTCACGCCACATATGGGCAAGATCGATCTTTACAAAACGAGCGGGCACTGGTATAC
CTATCGCGATGGCATTTTCCCGCCGATGCGCGAAATTGAAAATGATGACACGGAAAATCCAGAGGGCGAAATTTATCTGC
TCAAGCCGATGAACTGCCCGCATCATATTGAAATTTATGCTAGCGAACCACGTTCGTATCGCGATTTGCCCTTGCGCTTG
GCCGAATTTGGTACGGTGTATCGCTACGAACATAGTGGCGAATTAACGGGTTTGCTGCGGGTACGCTCGTTCACGGTCGA
TGATTCGCACCTGTTCGTCACGCCCGATCAGCTCGAAGCAGAATTTTTGAAAGTCGTTGACTTGATTCTCTTCGTGTTTG
GCACGATGGGCTTGAAGGATTTCCAAGCGCGGGTCGGCTTGCGCGAGGTGGGCAATCCCAAGTACATCGGTTCGGATGAG
GTCTGGGAAAAAGCTCAAAATGCGATCATCAATGCTGCCGAAAAGAAAGGCCTCAATTATATTGTGGTCGAAGGCGAAGC
AGCATTCTATGGGCCAAAACTCGACTTTATCTTCCGTGATGTGCTTGGTCGCCAGTGGCAGCTTGGCACGGTGCAGGTCG
ATTACAACTTGCCTGAGCGCTTTGAAATTGAGTACACGGGCGAAGATGGCCAAAAGCATCGGCCAATTATGATTCACCGT
GCGCCGTTTGGCTCGATCGAGCGCTTTGTGGGCACGTTGATCGAACAATATGCAGGGGCGTTTCCGGTCTGGTTGGCTCC
AGTTCAAGTAACGTTAGTGCCAATTACCGATCGGCATGTGGCCTATGCGGAGTCGGTGGCGGCCAAATTGAATGCTCAAG
GCTTGCGGGTTGAAGTTGATGCCAGCAACAACCGGATGAATGCTAAGATTCGCGATGCCCAAAAGCTGAAGATTCCGTAT
ATGTTGGTGGTGGGCGATAAAGAAGAGGAAGCGGGAGCTGTGGCGGTGCGTCAACGCTCTGGTGGCGACCTCGGCTCCAT
TAGCGTCGATGAGTTCATCGCGCGGATCAAGGACGAAGTTGCCAATTACCAATAA

Upstream 100 bases:

>100_bases
AAGCTTTTATAAAACCTTCTGATCAGTCAATAACCAAAAATTAACATCGTAGGCATTTTGCCTTTTGACCTTTGCCTTTT
GACTTTCTAGGAGTTTTCTT

Downstream 100 bases:

>100_bases
TTAAGCGAAGCCCCGCGATCAGTGTTCAGCCATTGGTCGCGGGGCTTTTTTTATTTAATGGACTAAAAACTGACAGGCGT
TTGGTACAACACCAAACGCC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MPPVNPDNDPLYRLRHSTAHVLAQAVLEIFPEGKIAIGPPVENGFYYDFDLPRPLTPDDLKDIEAKMRKIIKAKHRFAYR
EVSADEARELFKNQPYKLELIAGLAKGEDEYGEKAAASDTIISTYKHDSFEDLCKGPHVESLGDIPPNGFKLLRVSGAYW
RGDEKRPMLQRIYGTVWPSKEELDHYLWQQEEAKKRDHRKLGRELGLFTFSQKVGAGLALWLPKGAILRDVLERFLRQAQ
IERGYLPVVTPHMGKIDLYKTSGHWYTYRDGIFPPMREIENDDTENPEGEIYLLKPMNCPHHIEIYASEPRSYRDLPLRL
AEFGTVYRYEHSGELTGLLRVRSFTVDDSHLFVTPDQLEAEFLKVVDLILFVFGTMGLKDFQARVGLREVGNPKYIGSDE
VWEKAQNAIINAAEKKGLNYIVVEGEAAFYGPKLDFIFRDVLGRQWQLGTVQVDYNLPERFEIEYTGEDGQKHRPIMIHR
APFGSIERFVGTLIEQYAGAFPVWLAPVQVTLVPITDRHVAYAESVAAKLNAQGLRVEVDASNNRMNAKIRDAQKLKIPY
MLVVGDKEEEAGAVAVRQRSGGDLGSISVDEFIARIKDEVANYQ

Sequences:

>Translated_604_residues
MPPVNPDNDPLYRLRHSTAHVLAQAVLEIFPEGKIAIGPPVENGFYYDFDLPRPLTPDDLKDIEAKMRKIIKAKHRFAYR
EVSADEARELFKNQPYKLELIAGLAKGEDEYGEKAAASDTIISTYKHDSFEDLCKGPHVESLGDIPPNGFKLLRVSGAYW
RGDEKRPMLQRIYGTVWPSKEELDHYLWQQEEAKKRDHRKLGRELGLFTFSQKVGAGLALWLPKGAILRDVLERFLRQAQ
IERGYLPVVTPHMGKIDLYKTSGHWYTYRDGIFPPMREIENDDTENPEGEIYLLKPMNCPHHIEIYASEPRSYRDLPLRL
AEFGTVYRYEHSGELTGLLRVRSFTVDDSHLFVTPDQLEAEFLKVVDLILFVFGTMGLKDFQARVGLREVGNPKYIGSDE
VWEKAQNAIINAAEKKGLNYIVVEGEAAFYGPKLDFIFRDVLGRQWQLGTVQVDYNLPERFEIEYTGEDGQKHRPIMIHR
APFGSIERFVGTLIEQYAGAFPVWLAPVQVTLVPITDRHVAYAESVAAKLNAQGLRVEVDASNNRMNAKIRDAQKLKIPY
MLVVGDKEEEAGAVAVRQRSGGDLGSISVDEFIARIKDEVANYQ
>Mature_603_residues
PPVNPDNDPLYRLRHSTAHVLAQAVLEIFPEGKIAIGPPVENGFYYDFDLPRPLTPDDLKDIEAKMRKIIKAKHRFAYRE
VSADEARELFKNQPYKLELIAGLAKGEDEYGEKAAASDTIISTYKHDSFEDLCKGPHVESLGDIPPNGFKLLRVSGAYWR
GDEKRPMLQRIYGTVWPSKEELDHYLWQQEEAKKRDHRKLGRELGLFTFSQKVGAGLALWLPKGAILRDVLERFLRQAQI
ERGYLPVVTPHMGKIDLYKTSGHWYTYRDGIFPPMREIENDDTENPEGEIYLLKPMNCPHHIEIYASEPRSYRDLPLRLA
EFGTVYRYEHSGELTGLLRVRSFTVDDSHLFVTPDQLEAEFLKVVDLILFVFGTMGLKDFQARVGLREVGNPKYIGSDEV
WEKAQNAIINAAEKKGLNYIVVEGEAAFYGPKLDFIFRDVLGRQWQLGTVQVDYNLPERFEIEYTGEDGQKHRPIMIHRA
PFGSIERFVGTLIEQYAGAFPVWLAPVQVTLVPITDRHVAYAESVAAKLNAQGLRVEVDASNNRMNAKIRDAQKLKIPYM
LVVGDKEEEAGAVAVRQRSGGDLGSISVDEFIARIKDEVANYQ

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=586, Percent_Identity=37.3720136518771, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI38202255, Length=585, Percent_Identity=36.9230769230769, Blast_Score=391, Evalue=1e-108,
Organism=Homo sapiens, GI20070344, Length=591, Percent_Identity=35.8714043993232, Blast_Score=359, Evalue=4e-99,
Organism=Escherichia coli, GI1788013, Length=596, Percent_Identity=41.9463087248322, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71984184, Length=584, Percent_Identity=36.3013698630137, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71984192, Length=584, Percent_Identity=36.3013698630137, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322112, Length=601, Percent_Identity=35.4409317803661, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322654, Length=392, Percent_Identity=34.4387755102041, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24583842, Length=593, Percent_Identity=37.4367622259696, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI28574141, Length=593, Percent_Identity=37.4367622259696, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24583839, Length=593, Percent_Identity=37.4367622259696, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 68796; Mature: 68665

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVNPDNDPLYRLRHSTAHVLAQAVLEIFPEGKIAIGPPVENGFYYDFDLPRPLTPDDL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCCCCCCCCHHH
KDIEAKMRKIIKAKHRFAYREVSADEARELFKNQPYKLELIAGLAKGEDEYGEKAAASDT
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEEHHCCCHHHCCHHHCCCH
IISTYKHDSFEDLCKGPHVESLGDIPPNGFKLLRVSGAYWRGDEKRPMLQRIYGTVWPSK
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCEECCCCCCHHHHHHHHCCCCCH
EELDHYLWQQEEAKKRDHRKLGRELGLFTFSQKVGAGLALWLPKGAILRDVLERFLRQAQ
HHHHHHHHCHHHHHHHHHHHHHHHHCCEEEHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
IERGYLPVVTPHMGKIDLYKTSGHWYTYRDGIFPPMREIENDDTENPEGEIYLLKPMNCP
HHCCCCCEECCCCCEEEEEEECCCEEEECCCCCCCHHHHCCCCCCCCCCCEEEECCCCCC
HHIEIYASEPRSYRDLPLRLAEFGTVYRYEHSGELTGLLRVRSFTVDDSHLFVTPDQLEA
CEEEEEECCCCCCCCCCHHHHHCCCEEEEECCCCEEEEEEEEEEEECCCEEEECCHHHHH
EFLKVVDLILFVFGTMGLKDFQARVGLREVGNPKYIGSDEVWEKAQNAIINAAEKKGLNY
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
IVVEGEAAFYGPKLDFIFRDVLGRQWQLGTVQVDYNLPERFEIEYTGEDGQKHRPIMIHR
EEEECCCEEECCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEE
APFGSIERFVGTLIEQYAGAFPVWLAPVQVTLVPITDRHVAYAESVAAKLNAQGLRVEVD
CCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEE
ASNNRMNAKIRDAQKLKIPYMLVVGDKEEEAGAVAVRQRSGGDLGSISVDEFIARIKDEV
CCCCEEEEEECCCCEECCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ANYQ
CCCC
>Mature Secondary Structure 
PPVNPDNDPLYRLRHSTAHVLAQAVLEIFPEGKIAIGPPVENGFYYDFDLPRPLTPDDL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCCCCCCCCHHH
KDIEAKMRKIIKAKHRFAYREVSADEARELFKNQPYKLELIAGLAKGEDEYGEKAAASDT
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEEHHCCCHHHCCHHHCCCH
IISTYKHDSFEDLCKGPHVESLGDIPPNGFKLLRVSGAYWRGDEKRPMLQRIYGTVWPSK
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCEECCCCCCHHHHHHHHCCCCCH
EELDHYLWQQEEAKKRDHRKLGRELGLFTFSQKVGAGLALWLPKGAILRDVLERFLRQAQ
HHHHHHHHCHHHHHHHHHHHHHHHHCCEEEHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
IERGYLPVVTPHMGKIDLYKTSGHWYTYRDGIFPPMREIENDDTENPEGEIYLLKPMNCP
HHCCCCCEECCCCCEEEEEEECCCEEEECCCCCCCHHHHCCCCCCCCCCCEEEECCCCCC
HHIEIYASEPRSYRDLPLRLAEFGTVYRYEHSGELTGLLRVRSFTVDDSHLFVTPDQLEA
CEEEEEECCCCCCCCCCHHHHHCCCEEEEECCCCEEEEEEEEEEEECCCEEEECCHHHHH
EFLKVVDLILFVFGTMGLKDFQARVGLREVGNPKYIGSDEVWEKAQNAIINAAEKKGLNY
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
IVVEGEAAFYGPKLDFIFRDVLGRQWQLGTVQVDYNLPERFEIEYTGEDGQKHRPIMIHR
EEEECCCEEECCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEE
APFGSIERFVGTLIEQYAGAFPVWLAPVQVTLVPITDRHVAYAESVAAKLNAQGLRVEVD
CCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEE
ASNNRMNAKIRDAQKLKIPYMLVVGDKEEEAGAVAVRQRSGGDLGSISVDEFIARIKDEV
CCCCEEEEEECCCCEECCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ANYQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]