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The map label for this gene is queA [H]

Identifier: 159899314

GI number: 159899314

Start: 3553763

End: 3554803

Strand: Reverse

Name: queA [H]

Synonym: Haur_2795

Alternate gene names: 159899314

Gene position: 3554803-3553763 (Counterclockwise)

Preceding gene: 159899317

Following gene: 159899309

Centisome position: 56.01

GC content: 51.2

Gene sequence:

>1041_bases
ATGCTACCAATTGCCGATTTTGATTATCATTTGCCACCTGAACGCATCGCCCAAACCCCAATTGAGCCACGCGACCATTC
GCGTTTGTTGGTGCTCGACCGCGCTACAGGCCAGCTTGAGCATCGCCATTTTTATGAGATTGGGCGTTATTTGCGGCCTG
GCGACCTACTGGTTGCCAACGATAGTCGGGTTTTGCCCGCCCGTTTGTATGGCCAAAAAGCCAGTGGTGGCAAAACCGAG
TTGCTACTACTCAAACAACGCGATGCCCAACGCTGGGAAGCCTTGATCAAAGGCAAAGTTGCGGTTGGCACACGCTTAAT
CATCACTGGCCATCGTGGAGCCCAAGTCCAAGCAACAGTTGAGGAATTATTGGAAAGCGGCAGTCGAATTATCTGTTTTG
AGCAACCGATTAATGATCGATTGCCCGAACTTGGCTCAATGCCAACCCCACCCTATATCACTGCCCCGCTGACTGATCCT
GAGCGTTACCAAACAGTCTATAGCGCTCAAGCCGGCTCAGCCGCCGCCCCAACCGCAGGTTTACACTGGACACCAGCATT
AATCGAGCAGGTTAAAGCGCTGGGGGTTGGTTGGGCTAGCGTTACCTTACATGTTGGCTTGGATACATTTCGGCCTGTAC
AAACTGAAAACGCGCTTGATCATCAAATTCATAGTGAATGGTATGAGCTTTCGGCGGAAACTGCCCAAGCAATCAACGCA
ACCCGCGCTGCTGGCGGGAGAATTATCACGGTTGGTACCACCAGCACCCGTGTGCTTGAAACCGTGTCCCAATCCCAAGA
CGTTAGTTTGACTGCTGGCGAAATCCAAGCAGCGGCGGGCTGGAGCAGTATTTATATCTACCCGCCCTATGAATATCGAG
TGGTCAATAGTTTGATCACCAATTTTCACTTACCAAAATCGACCTTGTTGCTCTTGGTCAGTGCCTTGGCAAGCCGCGAT
TTGATTATGCAAGCCTATGCCGAGGCGGTCGAACGTCAGTATCGATTCTTCTCGTTTGGCGATGCCATGCTGATTATTTA
G

Upstream 100 bases:

>100_bases
TTCATTCCTCATCCCTCATCCTTATATTCTATGGTATGCTATAAACCCTAAACGTATGCTTTATCTATAGCCTATCGCCC
ATAGTCTATAGCCAATCATC

Downstream 100 bases:

>100_bases
GGCTGATCCACGAACGTTTATGCGTGCCTAATTAATGATTAACGGAGAGGCGCAGAGAATATAGCGCTATTTTTCTCTGC
GCCTCTCCGTTAAATCCAGT

Product: S-adenosylmethionine--tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MLPIADFDYHLPPERIAQTPIEPRDHSRLLVLDRATGQLEHRHFYEIGRYLRPGDLLVANDSRVLPARLYGQKASGGKTE
LLLLKQRDAQRWEALIKGKVAVGTRLIITGHRGAQVQATVEELLESGSRIICFEQPINDRLPELGSMPTPPYITAPLTDP
ERYQTVYSAQAGSAAAPTAGLHWTPALIEQVKALGVGWASVTLHVGLDTFRPVQTENALDHQIHSEWYELSAETAQAINA
TRAAGGRIITVGTTSTRVLETVSQSQDVSLTAGEIQAAAGWSSIYIYPPYEYRVVNSLITNFHLPKSTLLLLVSALASRD
LIMQAYAEAVERQYRFFSFGDAMLII

Sequences:

>Translated_346_residues
MLPIADFDYHLPPERIAQTPIEPRDHSRLLVLDRATGQLEHRHFYEIGRYLRPGDLLVANDSRVLPARLYGQKASGGKTE
LLLLKQRDAQRWEALIKGKVAVGTRLIITGHRGAQVQATVEELLESGSRIICFEQPINDRLPELGSMPTPPYITAPLTDP
ERYQTVYSAQAGSAAAPTAGLHWTPALIEQVKALGVGWASVTLHVGLDTFRPVQTENALDHQIHSEWYELSAETAQAINA
TRAAGGRIITVGTTSTRVLETVSQSQDVSLTAGEIQAAAGWSSIYIYPPYEYRVVNSLITNFHLPKSTLLLLVSALASRD
LIMQAYAEAVERQYRFFSFGDAMLII
>Mature_346_residues
MLPIADFDYHLPPERIAQTPIEPRDHSRLLVLDRATGQLEHRHFYEIGRYLRPGDLLVANDSRVLPARLYGQKASGGKTE
LLLLKQRDAQRWEALIKGKVAVGTRLIITGHRGAQVQATVEELLESGSRIICFEQPINDRLPELGSMPTPPYITAPLTDP
ERYQTVYSAQAGSAAAPTAGLHWTPALIEQVKALGVGWASVTLHVGLDTFRPVQTENALDHQIHSEWYELSAETAQAINA
TRAAGGRIITVGTTSTRVLETVSQSQDVSLTAGEIQAAAGWSSIYIYPPYEYRVVNSLITNFHLPKSTLLLLVSALASRD
LIMQAYAEAVERQYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=352, Percent_Identity=44.6022727272727, Blast_Score=279, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38237; Mature: 38237

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPIADFDYHLPPERIAQTPIEPRDHSRLLVLDRATGQLEHRHFYEIGRYLRPGDLLVAN
CCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEC
DSRVLPARLYGQKASGGKTELLLLKQRDAQRWEALIKGKVAVGTRLIITGHRGAQVQATV
CCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHCCCEEECEEEEEECCCCCCHHHHH
EELLESGSRIICFEQPINDRLPELGSMPTPPYITAPLTDPERYQTVYSAQAGSAAAPTAG
HHHHHCCCEEEEEECCCCCCCHHHCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCC
LHWTPALIEQVKALGVGWASVTLHVGLDTFRPVQTENALDHQIHSEWYELSAETAQAINA
CCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRAAGGRIITVGTTSTRVLETVSQSQDVSLTAGEIQAAAGWSSIYIYPPYEYRVVNSLIT
HHCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCEEECCCCCEEEEECCHHHHHHHHHHH
NFHLPKSTLLLLVSALASRDLIMQAYAEAVERQYRFFSFGDAMLII
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure
MLPIADFDYHLPPERIAQTPIEPRDHSRLLVLDRATGQLEHRHFYEIGRYLRPGDLLVAN
CCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEC
DSRVLPARLYGQKASGGKTELLLLKQRDAQRWEALIKGKVAVGTRLIITGHRGAQVQATV
CCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHCCCEEECEEEEEECCCCCCHHHHH
EELLESGSRIICFEQPINDRLPELGSMPTPPYITAPLTDPERYQTVYSAQAGSAAAPTAG
HHHHHCCCEEEEEECCCCCCCHHHCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCC
LHWTPALIEQVKALGVGWASVTLHVGLDTFRPVQTENALDHQIHSEWYELSAETAQAINA
CCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRAAGGRIITVGTTSTRVLETVSQSQDVSLTAGEIQAAAGWSSIYIYPPYEYRVVNSLIT
HHCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCEEECCCCCEEEEECCHHHHHHHHHHH
NFHLPKSTLLLLVSALASRDLIMQAYAEAVERQYRFFSFGDAMLII
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA