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The map label for this gene is nth [H]

Identifier: 159898923

GI number: 159898923

Start: 3061064

End: 3061732

Strand: Reverse

Name: nth [H]

Synonym: Haur_2402

Alternate gene names: 159898923

Gene position: 3061732-3061064 (Counterclockwise)

Preceding gene: 159898924

Following gene: 159898922

Centisome position: 48.24

GC content: 52.17

Gene sequence:

>669_bases
ATGCTCAACCCTGAGAAAGTGCTTGAAACGTTACACATATTGCGTGAACGCTTTGGTCCACGCGTCTTGCATACTCAACG
TGATCCACTTGATGAGCTGGTTTTGACGATTCTTTCGCAAAATACTTCCGACCGTAATAGTGGTCGGGCTTTTCGCGAAC
TCAAAGGGCGCTACCCAACGTGGGCGGCAGTGCTCAACGCCGAATCAAGCGAGTTAGAGGAAACAATTCGCGTTGGTGGG
TTGGCTAAAATCAAGGCGGCGCGGATTCAAAATACCTTGGCGGTAATTTTGGAGCAACGCGGCGAATTTAGCCTCGATTT
TCTGCGCGAGCTAGGTTTGCACGAAGCGCGGGCTTGGCTGACCGCACTGCCGGGCATTGGCCCCAAAACGGCGGGCTGTG
TGCTCTGTTTTGCCTGCAATCAGCCCGCCATGATCGTCGATACCCACATTCATCGCGTGGCCAAACGGGTTGGCATGATT
GGGCCTAAAGTTTCTGCCGATGCTGCCCACGATTTGCTTGAGTCGGCTGTGCCAGTTGATCAAATGTATCAATTTCATGT
CAGTGTGTTGTTGCATGGCCGCCAAATTTGCCATGCCCAACGCCCAGCCTGCGAGCGCTGCCCATTAACCGAGATTTGCG
ATTTTTATCAAGCCAATTCAACATCATAG

Upstream 100 bases:

>100_bases
GCAGCGTGGTTCAGGCCTCGCCAATGTATCTGATTTGGGTTCCATAAGATTGATTGCTATGGTAGGCTAAGAAGACAAGT
GCTAGTTAGAAGGAACAGTG

Downstream 100 bases:

>100_bases
CTGGACTTGATGTAGGTCGATTAGCACGAATGTGATCAACAAAAACGATTGCGTTCAGCAGAAATGCGCCACTCCAAGCA
GTTTGTTCGCCAATAAAACA

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MLNPEKVLETLHILRERFGPRVLHTQRDPLDELVLTILSQNTSDRNSGRAFRELKGRYPTWAAVLNAESSELEETIRVGG
LAKIKAARIQNTLAVILEQRGEFSLDFLRELGLHEARAWLTALPGIGPKTAGCVLCFACNQPAMIVDTHIHRVAKRVGMI
GPKVSADAAHDLLESAVPVDQMYQFHVSVLLHGRQICHAQRPACERCPLTEICDFYQANSTS

Sequences:

>Translated_222_residues
MLNPEKVLETLHILRERFGPRVLHTQRDPLDELVLTILSQNTSDRNSGRAFRELKGRYPTWAAVLNAESSELEETIRVGG
LAKIKAARIQNTLAVILEQRGEFSLDFLRELGLHEARAWLTALPGIGPKTAGCVLCFACNQPAMIVDTHIHRVAKRVGMI
GPKVSADAAHDLLESAVPVDQMYQFHVSVLLHGRQICHAQRPACERCPLTEICDFYQANSTS
>Mature_222_residues
MLNPEKVLETLHILRERFGPRVLHTQRDPLDELVLTILSQNTSDRNSGRAFRELKGRYPTWAAVLNAESSELEETIRVGG
LAKIKAARIQNTLAVILEQRGEFSLDFLRELGLHEARAWLTALPGIGPKTAGCVLCFACNQPAMIVDTHIHRVAKRVGMI
GPKVSADAAHDLLESAVPVDQMYQFHVSVLLHGRQICHAQRPACERCPLTEICDFYQANSTS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=218, Percent_Identity=32.5688073394495, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554540, Length=191, Percent_Identity=29.3193717277487, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550361, Length=191, Percent_Identity=28.7958115183246, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24727; Mature: 24727

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPEKVLETLHILRERFGPRVLHTQRDPLDELVLTILSQNTSDRNSGRAFRELKGRYPT
CCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCH
WAAVLNAESSELEETIRVGGLAKIKAARIQNTLAVILEQRGEFSLDFLRELGLHEARAWL
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHH
TALPGIGPKTAGCVLCFACNQPAMIVDTHIHRVAKRVGMIGPKVSADAAHDLLESAVPVD
HHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
QMYQFHVSVLLHGRQICHAQRPACERCPLTEICDFYQANSTS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNPEKVLETLHILRERFGPRVLHTQRDPLDELVLTILSQNTSDRNSGRAFRELKGRYPT
CCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCH
WAAVLNAESSELEETIRVGGLAKIKAARIQNTLAVILEQRGEFSLDFLRELGLHEARAWL
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHH
TALPGIGPKTAGCVLCFACNQPAMIVDTHIHRVAKRVGMIGPKVSADAAHDLLESAVPVD
HHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
QMYQFHVSVLLHGRQICHAQRPACERCPLTEICDFYQANSTS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]