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The map label for this gene is pleD [H]

Identifier: 159898911

GI number: 159898911

Start: 3044885

End: 3046687

Strand: Reverse

Name: pleD [H]

Synonym: Haur_2390

Alternate gene names: 159898911

Gene position: 3046687-3044885 (Counterclockwise)

Preceding gene: 159898912

Following gene: 159898909

Centisome position: 48.01

GC content: 46.59

Gene sequence:

>1803_bases
ATGAATCATGCAACAACTTTAGCGGCCTTACATGCTGCATTGGCCGAAATTCATAATCCAATGCAGCATGTCGATGTACT
TAACCGATTAGTTGCCGAACTCCGTTTAGTTGACCATGAACAGGCTTGGGCACTCAACCATCAGGCCGAAGGTTTGGCGC
GGATGGCGGGCGACGATGGCAAGCCTTATACTCAAGGGTTGATCGATTGTTTCTATTGGCGTTCTAAACTGCTGTTTAGC
GAACAATCGTATCATCAGGCGTTAACCTACCTCTTTCGCGCCAGCAAACTGTGCCAATTAACCCCGCCCCGCCAGCCTAA
CCCGCATTGGCTTGATATCTTAGATACCAGTTTTCAATCAGCCTCGCTTGGCTCAGAAGATAGTGATGCATTTTTAGCTA
GCGGCATTCACAGCGCGGCGGTACATAATGCCTTGGGCGTGTTGTTGATTGTGCTGGGCAACTATACCGAAGCTTTGCAA
TATTTGCAGCGGGCCAAACGCGATGCGATCCAATTAAATGATCAATGGTTTGAGGCTTTGGTCTGCAATAATTTGGGCTT
TTTGCATTGCGAATTGCAAAGCCCCGAAATTGCCCTGAATGATCTTGCCCATGGTTTACGTTTGCTTGAAGCCATGGCCC
AATTTCCGGCGCATCTGCGCATGCGAGCCGAAATTCTCGATAACAGCGGGCGGGCGGCGTGTGCCTTAAAGCAATTTGAG
CAAGCTGTGAGCTATGGTGAGCAAGCACTGGCACTCTATCACGAAATCGATTGGCAACATGGCCAGGCCGAGTGTCATAA
TAATTTGGGTTTAGCGTATGCTGGGCTTGATCAACAAAATCGAGCAATTGAGCATTTTATGCAAGCCTATGAATTAGCTT
GTGGCTCGAACAATATTCACGAAGCAATTGAAGCCTTGATTCGCCATGGAGTTTTGCTTAATCGAATTAAACATTATCAA
GCCGCCCGCGTGATTCTTGAGCAAGCTTTGATTTCAGTCAATCCTACAATCAATCAACAAAAAGTTTATCGGGCACATCA
AGCTTTGGCTGATACTTATGAGGCTTTGGGTGATTTTAAAGCCTCGTTGAAGCACTATAAAGCCTATTATGAATTAAAAG
ATACGGTTTTTAATGAGCAATCAGTGCGACGAATGCAAGTGTTGCAAACGATGTATCGTTTAGATGAAGCTCGTAATCAA
GCAGCGCTGTATCATTTGCAAAATGATGATTTACATCAACAATTAGCTGATATTCAACAAATTAATTTGCAATTGGCTGA
ATGGGCTAAAACTGACTCACTGACCGAAATTCTCAATCGACGCGGCTTATACGAACAAATCGACCTCGCTTTGCAACATC
AACAAACTGATGCCCAAGGCTGGGCTTGGATTATGTTTGATATTGATTTTTTCAAGGCGATTAATGATAGCTATGGCCAC
GATAGCGGCGACGAGGTGCTGCGCGGGATTGCCCAACGAGCGCGATTGGTGCTGCGCCATAGCGATGTCTTTGGGCGCTA
TGGCGGTGAGGAATTTTTGGTCTTTTTGCCGCATACCTCGATGCAAGAAGCCATTGTGATTGCCGAACGCTTGCGCACGG
CGATTGCCAACACGCCGTATGTGGTTAAGCAAACTGCCGTCAATGTGACAATTAGCCTTGGAATTGCCTGGACAGAGCAA
GTTGATGACCCTGAACGCCTGACCCAATTAGCCGATCAAGCGTTGTATCGTTCCAAGGCGGCGGGGCGCAATTGTTATAC
AATTGAGCGTTTTGATGCGCCAGTTAGCCAACTTTCAGCGTGA

Upstream 100 bases:

>100_bases
AAAGCTATAGCTCAAAAACACCACTGAAGAGTTGCATCAATTGCCATGAAATACTATGATGCATGCAACGAATTTTTAAT
CATTACACTGCGTGGCAAGC

Downstream 100 bases:

>100_bases
TGCGTTGCTCAGAGGCGATTGCTAGTGTATTGGCAAGCGTCAGCAAATGTTCGTTGATATAGGCGCGATCGAATGGCTCA
GTGATGGCTTGATCGGCTCC

Product: TPR repeat-containing diguanylate cyclase and serine/threonine protein kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MNHATTLAALHAALAEIHNPMQHVDVLNRLVAELRLVDHEQAWALNHQAEGLARMAGDDGKPYTQGLIDCFYWRSKLLFS
EQSYHQALTYLFRASKLCQLTPPRQPNPHWLDILDTSFQSASLGSEDSDAFLASGIHSAAVHNALGVLLIVLGNYTEALQ
YLQRAKRDAIQLNDQWFEALVCNNLGFLHCELQSPEIALNDLAHGLRLLEAMAQFPAHLRMRAEILDNSGRAACALKQFE
QAVSYGEQALALYHEIDWQHGQAECHNNLGLAYAGLDQQNRAIEHFMQAYELACGSNNIHEAIEALIRHGVLLNRIKHYQ
AARVILEQALISVNPTINQQKVYRAHQALADTYEALGDFKASLKHYKAYYELKDTVFNEQSVRRMQVLQTMYRLDEARNQ
AALYHLQNDDLHQQLADIQQINLQLAEWAKTDSLTEILNRRGLYEQIDLALQHQQTDAQGWAWIMFDIDFFKAINDSYGH
DSGDEVLRGIAQRARLVLRHSDVFGRYGGEEFLVFLPHTSMQEAIVIAERLRTAIANTPYVVKQTAVNVTISLGIAWTEQ
VDDPERLTQLADQALYRSKAAGRNCYTIERFDAPVSQLSA

Sequences:

>Translated_600_residues
MNHATTLAALHAALAEIHNPMQHVDVLNRLVAELRLVDHEQAWALNHQAEGLARMAGDDGKPYTQGLIDCFYWRSKLLFS
EQSYHQALTYLFRASKLCQLTPPRQPNPHWLDILDTSFQSASLGSEDSDAFLASGIHSAAVHNALGVLLIVLGNYTEALQ
YLQRAKRDAIQLNDQWFEALVCNNLGFLHCELQSPEIALNDLAHGLRLLEAMAQFPAHLRMRAEILDNSGRAACALKQFE
QAVSYGEQALALYHEIDWQHGQAECHNNLGLAYAGLDQQNRAIEHFMQAYELACGSNNIHEAIEALIRHGVLLNRIKHYQ
AARVILEQALISVNPTINQQKVYRAHQALADTYEALGDFKASLKHYKAYYELKDTVFNEQSVRRMQVLQTMYRLDEARNQ
AALYHLQNDDLHQQLADIQQINLQLAEWAKTDSLTEILNRRGLYEQIDLALQHQQTDAQGWAWIMFDIDFFKAINDSYGH
DSGDEVLRGIAQRARLVLRHSDVFGRYGGEEFLVFLPHTSMQEAIVIAERLRTAIANTPYVVKQTAVNVTISLGIAWTEQ
VDDPERLTQLADQALYRSKAAGRNCYTIERFDAPVSQLSA
>Mature_600_residues
MNHATTLAALHAALAEIHNPMQHVDVLNRLVAELRLVDHEQAWALNHQAEGLARMAGDDGKPYTQGLIDCFYWRSKLLFS
EQSYHQALTYLFRASKLCQLTPPRQPNPHWLDILDTSFQSASLGSEDSDAFLASGIHSAAVHNALGVLLIVLGNYTEALQ
YLQRAKRDAIQLNDQWFEALVCNNLGFLHCELQSPEIALNDLAHGLRLLEAMAQFPAHLRMRAEILDNSGRAACALKQFE
QAVSYGEQALALYHEIDWQHGQAECHNNLGLAYAGLDQQNRAIEHFMQAYELACGSNNIHEAIEALIRHGVLLNRIKHYQ
AARVILEQALISVNPTINQQKVYRAHQALADTYEALGDFKASLKHYKAYYELKDTVFNEQSVRRMQVLQTMYRLDEARNQ
AALYHLQNDDLHQQLADIQQINLQLAEWAKTDSLTEILNRRGLYEQIDLALQHQQTDAQGWAWIMFDIDFFKAINDSYGH
DSGDEVLRGIAQRARLVLRHSDVFGRYGGEEFLVFLPHTSMQEAIVIAERLRTAIANTPYVVKQTAVNVTISLGIAWTEQ
VDDPERLTQLADQALYRSKAAGRNCYTIERFDAPVSQLSA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=40.5882352941176, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1787802, Length=183, Percent_Identity=33.879781420765, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=36.144578313253, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI145693134, Length=154, Percent_Identity=37.6623376623377, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=35.1851851851852, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=34.8066298342541, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=36.7088607594937, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=34.1614906832298, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787056, Length=170, Percent_Identity=28.8235294117647, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=31.7073170731707, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=176, Percent_Identity=28.9772727272727, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788956, Length=218, Percent_Identity=31.1926605504587, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 67929; Mature: 67929

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50005 TPR L=RR ; PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHATTLAALHAALAEIHNPMQHVDVLNRLVAELRLVDHEQAWALNHQAEGLARMAGDDG
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHCCCCC
KPYTQGLIDCFYWRSKLLFSEQSYHQALTYLFRASKLCQLTPPRQPNPHWLDILDTSFQS
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHCCCCC
ASLGSEDSDAFLASGIHSAAVHNALGVLLIVLGNYTEALQYLQRAKRDAIQLNDQWFEAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
VCNNLGFLHCELQSPEIALNDLAHGLRLLEAMAQFPAHLRMRAEILDNSGRAACALKQFE
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHH
QAVSYGEQALALYHEIDWQHGQAECHNNLGLAYAGLDQQNRAIEHFMQAYELACGSNNIH
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHH
EAIEALIRHGVLLNRIKHYQAARVILEQALISVNPTINQQKVYRAHQALADTYEALGDFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASLKHYKAYYELKDTVFNEQSVRRMQVLQTMYRLDEARNQAALYHLQNDDLHQQLADIQQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
INLQLAEWAKTDSLTEILNRRGLYEQIDLALQHQQTDAQGWAWIMFDIDFFKAINDSYGH
HHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHCCCCCC
DSGDEVLRGIAQRARLVLRHSDVFGRYGGEEFLVFLPHTSMQEAIVIAERLRTAIANTPY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
VVKQTAVNVTISLGIAWTEQVDDPERLTQLADQALYRSKAAGRNCYTIERFDAPVSQLSA
EEEEHEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHCC
>Mature Secondary Structure
MNHATTLAALHAALAEIHNPMQHVDVLNRLVAELRLVDHEQAWALNHQAEGLARMAGDDG
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHCCCCC
KPYTQGLIDCFYWRSKLLFSEQSYHQALTYLFRASKLCQLTPPRQPNPHWLDILDTSFQS
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHCCCCC
ASLGSEDSDAFLASGIHSAAVHNALGVLLIVLGNYTEALQYLQRAKRDAIQLNDQWFEAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
VCNNLGFLHCELQSPEIALNDLAHGLRLLEAMAQFPAHLRMRAEILDNSGRAACALKQFE
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHH
QAVSYGEQALALYHEIDWQHGQAECHNNLGLAYAGLDQQNRAIEHFMQAYELACGSNNIH
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHH
EAIEALIRHGVLLNRIKHYQAARVILEQALISVNPTINQQKVYRAHQALADTYEALGDFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASLKHYKAYYELKDTVFNEQSVRRMQVLQTMYRLDEARNQAALYHLQNDDLHQQLADIQQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
INLQLAEWAKTDSLTEILNRRGLYEQIDLALQHQQTDAQGWAWIMFDIDFFKAINDSYGH
HHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHCCCCCC
DSGDEVLRGIAQRARLVLRHSDVFGRYGGEEFLVFLPHTSMQEAIVIAERLRTAIANTPY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
VVKQTAVNVTISLGIAWTEQVDDPERLTQLADQALYRSKAAGRNCYTIERFDAPVSQLSA
EEEEHEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA