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The map label for this gene is metK [H]

Identifier: 159898874

GI number: 159898874

Start: 3002234

End: 3003451

Strand: Direct

Name: metK [H]

Synonym: Haur_2353

Alternate gene names: 159898874

Gene position: 3002234-3003451 (Clockwise)

Preceding gene: 159898873

Following gene: 159898875

Centisome position: 47.3

GC content: 51.48

Gene sequence:

>1218_bases
ATGGATGCAACTACGTTTATGCGTGCTCCACAGTTCTTCTTCACCTCAGAATCGGTGACTGAAGGACACCCCGATAAAAT
TTGTGACCAAGTTTCTGATGCGATCTTAGATGAATTGTTGGCCCAAGACCCCATGTCGCGGGTAGCTTGCGAAACCGCTA
CTACCACCGGCTTGATCGTCGTTCTCGGTGAAGTTACCACCAAGGGCTATGTTGAAGTTCAAGATATTGTGCGCAAAGTT
GTCAGCGATATTGGCTACACCCGTGGTAAGTTCGGCTTCGATGCCGAAACCTGTGGGATTATCGTCGCCTTGCACGGCCA
ATCGCCCGACATCGCTCAAGGCGTTGATGTGGCCTTGGAAGTACGCGACGATCAAGCCAGTCGCGAAGCTGAAATTGAAA
AAGTTGGCGCTGGCGATCAAGGTATGGTGTTCGGGTTCGCTTGTAACGAAACCGATGAATTTATGCCTTTGACGATTTCG
TTGGCTCACCAATTGACCCGCCGCTTGGCCAAAGTGCGCAAAACTGGCGAAGTTGGCTACTTACGCCCCGACGGCAAGAG
CCAAGTAACCGTCGAATATAGCCATGGCAAGCCTGTGCGCGTCGATACCGTGTTGATCTCAACCCAGCACGATCCCAACG
TCGATAACGAGCGGATTCACAAGGATGTAATCGAATTAGTGATTAAGCCGGTAATTCCTGAAGGCATGCTTGATGAAAAC
ACCAAGATTTTCATCAACCCAACTGGCCGCTTCGTCACTGGTGGCCCAATGGGCGATTCAGGTTTGACTGGCCGCAAGAT
CATCGTTGACACCTACGGCGGGGTTGCTCGCCACGGTGGCGGCGCTTTCTCAGGCAAAGATTCAACCAAGGTTGATCGCT
CAGCTGCCTATGCTGCTCGCTACGTTGCCAAAAACATCGTGGCTGCTGGCTTGGCCGAACGCTTTGAATTGCAAGTCAGC
TATGCAATCGGCGTTTCAAAGCCGCTTTCAATCTCGTTTGAAACCTTTGGTACCGCCAAAGTCAGCGACGAAAAATTGTT
AGAGTTGATCAACAAACACTTTGATTTGCGTCCAGGTGCGATCATCCGTGATCTTGATCTGCGCCGCCCAATCTATCGCC
AAACCGCTGCCTACGGTCACTTTGGCCGTGCTGACATCGATTTGCCATGGGAACGCACCGACAAAGCTGAATTGCTTCGC
GCTGAATCAGGCCTGTAA

Upstream 100 bases:

>100_bases
CAGTATATATTTTCAGCCTGTAGCAAAACCAATCCTCAGAGAACCCAATGAGTGTGGAACTCCAAGATTGGTTAACCATA
GATTTAGGAGATAAAACTCA

Downstream 100 bases:

>100_bases
GTCCAATCAATACCCTGCTGAGAGCTACCCGCAAGATGGTAGCTCTTGGCATTTTAGGAGGATCATGATTCAGATTCGAC
CTGAAACCAGCGCCGATTCC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MDATTFMRAPQFFFTSESVTEGHPDKICDQVSDAILDELLAQDPMSRVACETATTTGLIVVLGEVTTKGYVEVQDIVRKV
VSDIGYTRGKFGFDAETCGIIVALHGQSPDIAQGVDVALEVRDDQASREAEIEKVGAGDQGMVFGFACNETDEFMPLTIS
LAHQLTRRLAKVRKTGEVGYLRPDGKSQVTVEYSHGKPVRVDTVLISTQHDPNVDNERIHKDVIELVIKPVIPEGMLDEN
TKIFINPTGRFVTGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDSTKVDRSAAYAARYVAKNIVAAGLAERFELQVS
YAIGVSKPLSISFETFGTAKVSDEKLLELINKHFDLRPGAIIRDLDLRRPIYRQTAAYGHFGRADIDLPWERTDKAELLR
AESGL

Sequences:

>Translated_405_residues
MDATTFMRAPQFFFTSESVTEGHPDKICDQVSDAILDELLAQDPMSRVACETATTTGLIVVLGEVTTKGYVEVQDIVRKV
VSDIGYTRGKFGFDAETCGIIVALHGQSPDIAQGVDVALEVRDDQASREAEIEKVGAGDQGMVFGFACNETDEFMPLTIS
LAHQLTRRLAKVRKTGEVGYLRPDGKSQVTVEYSHGKPVRVDTVLISTQHDPNVDNERIHKDVIELVIKPVIPEGMLDEN
TKIFINPTGRFVTGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDSTKVDRSAAYAARYVAKNIVAAGLAERFELQVS
YAIGVSKPLSISFETFGTAKVSDEKLLELINKHFDLRPGAIIRDLDLRRPIYRQTAAYGHFGRADIDLPWERTDKAELLR
AESGL
>Mature_405_residues
MDATTFMRAPQFFFTSESVTEGHPDKICDQVSDAILDELLAQDPMSRVACETATTTGLIVVLGEVTTKGYVEVQDIVRKV
VSDIGYTRGKFGFDAETCGIIVALHGQSPDIAQGVDVALEVRDDQASREAEIEKVGAGDQGMVFGFACNETDEFMPLTIS
LAHQLTRRLAKVRKTGEVGYLRPDGKSQVTVEYSHGKPVRVDTVLISTQHDPNVDNERIHKDVIELVIKPVIPEGMLDEN
TKIFINPTGRFVTGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDSTKVDRSAAYAARYVAKNIVAAGLAERFELQVS
YAIGVSKPLSISFETFGTAKVSDEKLLELINKHFDLRPGAIIRDLDLRRPIYRQTAAYGHFGRADIDLPWERTDKAELLR
AESGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=390, Percent_Identity=56.4102564102564, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI4557737, Length=384, Percent_Identity=55.2083333333333, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=59.4936708860759, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI25145633, Length=404, Percent_Identity=55.4455445544555, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538498, Length=390, Percent_Identity=56.4102564102564, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=390, Percent_Identity=55.6410256410256, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=54.6632124352332, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=390, Percent_Identity=50.7692307692308, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145635, Length=353, Percent_Identity=53.5410764872521, Blast_Score=357, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=339, Percent_Identity=54.5722713864307, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=312, Percent_Identity=54.4871794871795, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=107, Percent_Identity=50.4672897196262, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=56.8831168831169, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320710, Length=395, Percent_Identity=54.9367088607595, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=391, Percent_Identity=53.9641943734015, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580529, Length=391, Percent_Identity=53.9641943734015, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580537, Length=391, Percent_Identity=53.9641943734015, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580533, Length=391, Percent_Identity=53.9641943734015, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580535, Length=391, Percent_Identity=53.9641943734015, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580531, Length=391, Percent_Identity=53.9641943734015, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=391, Percent_Identity=53.4526854219949, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=391, Percent_Identity=53.4526854219949, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=52.3809523809524, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580539, Length=129, Percent_Identity=51.937984496124, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44194; Mature: 44194

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDATTFMRAPQFFFTSESVTEGHPDKICDQVSDAILDELLAQDPMSRVACETATTTGLIV
CCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEE
VLGEVTTKGYVEVQDIVRKVVSDIGYTRGKFGFDAETCGIIVALHGQSPDIAQGVDVALE
EEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEE
VRDDQASREAEIEKVGAGDQGMVFGFACNETDEFMPLTISLAHQLTRRLAKVRKTGEVGY
ECCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEE
LRPDGKSQVTVEYSHGKPVRVDTVLISTQHDPNVDNERIHKDVIELVIKPVIPEGMLDEN
ECCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TKIFINPTGRFVTGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDSTKVDRSAAYAAR
CEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHH
YVAKNIVAAGLAERFELQVSYAIGVSKPLSISFETFGTAKVSDEKLLELINKHFDLRPGA
HHHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
IIRDLDLRRPIYRQTAAYGHFGRADIDLPWERTDKAELLRAESGL
EEECCHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MDATTFMRAPQFFFTSESVTEGHPDKICDQVSDAILDELLAQDPMSRVACETATTTGLIV
CCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEE
VLGEVTTKGYVEVQDIVRKVVSDIGYTRGKFGFDAETCGIIVALHGQSPDIAQGVDVALE
EEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEE
VRDDQASREAEIEKVGAGDQGMVFGFACNETDEFMPLTISLAHQLTRRLAKVRKTGEVGY
ECCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEE
LRPDGKSQVTVEYSHGKPVRVDTVLISTQHDPNVDNERIHKDVIELVIKPVIPEGMLDEN
ECCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TKIFINPTGRFVTGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDSTKVDRSAAYAAR
CEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHH
YVAKNIVAAGLAERFELQVSYAIGVSKPLSISFETFGTAKVSDEKLLELINKHFDLRPGA
HHHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
IIRDLDLRRPIYRQTAAYGHFGRADIDLPWERTDKAELLRAESGL
EEECCHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA