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The map label for this gene is braC [H]

Identifier: 159898844

GI number: 159898844

Start: 2964601

End: 2965752

Strand: Reverse

Name: braC [H]

Synonym: Haur_2323

Alternate gene names: 159898844

Gene position: 2965752-2964601 (Counterclockwise)

Preceding gene: 159898845

Following gene: 159898843

Centisome position: 46.73

GC content: 48.26

Gene sequence:

>1152_bases
ATGCTAAGACGAACCGCATTGGGGATGCTGGTAATCATCGGTTTGGCCTTGGTTAGTGCGTGTGGGGGTGCTTCACAACC
TCAAGCCGAGACGATCAAAATCGGCTTTTTTGGCCCAATGAGCGGCAACAGTGCTAGCGATGGCACGCACTCGATGTATG
CTGCGAAATTGGCGATTGATCAAGCAAACGCCAACCAAACTGTGCCCGGCAAGACCTTCGAGTTGATAACCTATGATGAT
CAACTTGATCCCAATCAAGCAATTACGGTTGCCCAGAAACTCGTCACTGAAGATAAAGTTCGCGCCGTGATTGGCGGCTC
CTACAGCAGCACAACCCGCGCAGCCGCGCCAGTCTTCCAACAAGCTGGCATCCCCATGTTAGCGGCTTATGCCACCCATC
CTGAGGTTACCAAAACTGGTTCGATGATCTTTCGCGTCATTTATATAACGACAGTCCAAGGTAATGCATTGGCGGTTTAT
GCCACAACCAAGCTCAATTATCGTAATTTCCACGTGCTGAATATTAATAATGATTATGGGCGGAGCATCACCGAAGCCTT
TACCAAAACTGCTCAAGCCAATCATTCAACAATCGTCAGCGCGAGCGAATATGCCAGTGATGCCACTGATTTTAGCAGCC
AAATTGCGACGATTAAGGCCAAACCAGCCGATGCCTTGATTTTGGTGGGTTATTATGCCCAGAGTGCTGATATCATTAAA
CAAGTGCGTCAGGCAGGCATTACCTTGCCAATTATTGGCTCCGATGGCTTGGATTCGCCGTCGTTGGTCGAGCTTGGTGG
TAGCGATGTTGAAGGCGTGATTTTGGCAACCGATTTTTCGCGCAATGATCCACGATCAATTGTACAGAATTTTATTCGCG
AATATCGTAATGCCCACGGAATTGATCCCGACGTTGTAGCTTCATCAACCTATGATGCTACCAATTTGTTGATCGATGCC
TTCAAACGTAGCCCAGACAGCAGCTCAAGCGCGATTCGTGATACTTTGGCGGCAACCCAAACCTACGAAGGGGTGACGGG
CGCAATTGTCTTTACGCCTGAACGTGAAGTTAATAAAACCGTGCTGTTCGTCAAAATTGAACGAGGAGCCTTTACCTTTT
TGGATAAACTTGATCCTAGCCAATTGCCCTAA

Upstream 100 bases:

>100_bases
TTCTGTGTTACACTGGCCATGCAGCAGGGTGAGTAAATTAACATTGTTCTTGGGTAGTGGCCTACTAAGCCATGTGGTAG
CACATGGAGGAGTCTTGAAC

Downstream 100 bases:

>100_bases
ATCGATCTGAGCACGTCGTTCGATCCAACGTCGCAAAGCACCATTTCAGCAGAGGTAGGAATACATATGCGGGCACGGGT
TCGCCAACGAGTTACCTGGA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MLRRTALGMLVIIGLALVSACGGASQPQAETIKIGFFGPMSGNSASDGTHSMYAAKLAIDQANANQTVPGKTFELITYDD
QLDPNQAITVAQKLVTEDKVRAVIGGSYSSTTRAAAPVFQQAGIPMLAAYATHPEVTKTGSMIFRVIYITTVQGNALAVY
ATTKLNYRNFHVLNINNDYGRSITEAFTKTAQANHSTIVSASEYASDATDFSSQIATIKAKPADALILVGYYAQSADIIK
QVRQAGITLPIIGSDGLDSPSLVELGGSDVEGVILATDFSRNDPRSIVQNFIREYRNAHGIDPDVVASSTYDATNLLIDA
FKRSPDSSSSAIRDTLAATQTYEGVTGAIVFTPEREVNKTVLFVKIERGAFTFLDKLDPSQLP

Sequences:

>Translated_383_residues
MLRRTALGMLVIIGLALVSACGGASQPQAETIKIGFFGPMSGNSASDGTHSMYAAKLAIDQANANQTVPGKTFELITYDD
QLDPNQAITVAQKLVTEDKVRAVIGGSYSSTTRAAAPVFQQAGIPMLAAYATHPEVTKTGSMIFRVIYITTVQGNALAVY
ATTKLNYRNFHVLNINNDYGRSITEAFTKTAQANHSTIVSASEYASDATDFSSQIATIKAKPADALILVGYYAQSADIIK
QVRQAGITLPIIGSDGLDSPSLVELGGSDVEGVILATDFSRNDPRSIVQNFIREYRNAHGIDPDVVASSTYDATNLLIDA
FKRSPDSSSSAIRDTLAATQTYEGVTGAIVFTPEREVNKTVLFVKIERGAFTFLDKLDPSQLP
>Mature_383_residues
MLRRTALGMLVIIGLALVSACGGASQPQAETIKIGFFGPMSGNSASDGTHSMYAAKLAIDQANANQTVPGKTFELITYDD
QLDPNQAITVAQKLVTEDKVRAVIGGSYSSTTRAAAPVFQQAGIPMLAAYATHPEVTKTGSMIFRVIYITTVQGNALAVY
ATTKLNYRNFHVLNINNDYGRSITEAFTKTAQANHSTIVSASEYASDATDFSSQIATIKAKPADALILVGYYAQSADIIK
QVRQAGITLPIIGSDGLDSPSLVELGGSDVEGVILATDFSRNDPRSIVQNFIREYRNAHGIDPDVVASSTYDATNLLIDA
FKRSPDSSSSAIRDTLAATQTYEGVTGAIVFTPEREVNKTVLFVKIERGAFTFLDKLDPSQLP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=268, Percent_Identity=29.4776119402985, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789867, Length=248, Percent_Identity=29.4354838709677, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40923; Mature: 40923

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRTALGMLVIIGLALVSACGGASQPQAETIKIGFFGPMSGNSASDGTHSMYAAKLAID
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHEEEEEEEE
QANANQTVPGKTFELITYDDQLDPNQAITVAQKLVTEDKVRAVIGGSYSSTTRAAAPVFQ
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHH
QAGIPMLAAYATHPEVTKTGSMIFRVIYITTVQGNALAVYATTKLNYRNFHVLNINNDYG
HCCCCEEEEECCCCCHHHHHHEEEEEEEEEEECCCEEEEEEEEEECCCEEEEEECCCHHC
RSITEAFTKTAQANHSTIVSASEYASDATDFSSQIATIKAKPADALILVGYYAQSADIIK
HHHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHHEEEEEECCCCEEEEEEEECCCHHHHH
QVRQAGITLPIIGSDGLDSPSLVELGGSDVEGVILATDFSRNDPRSIVQNFIREYRNAHG
HHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
IDPDVVASSTYDATNLLIDAFKRSPDSSSSAIRDTLAATQTYEGVTGAIVFTPEREVNKT
CCHHHEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCE
VLFVKIERGAFTFLDKLDPSQLP
EEEEEECCCCEEHHHCCCCCCCC
>Mature Secondary Structure
MLRRTALGMLVIIGLALVSACGGASQPQAETIKIGFFGPMSGNSASDGTHSMYAAKLAID
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHEEEEEEEE
QANANQTVPGKTFELITYDDQLDPNQAITVAQKLVTEDKVRAVIGGSYSSTTRAAAPVFQ
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHH
QAGIPMLAAYATHPEVTKTGSMIFRVIYITTVQGNALAVYATTKLNYRNFHVLNINNDYG
HCCCCEEEEECCCCCHHHHHHEEEEEEEEEEECCCEEEEEEEEEECCCEEEEEECCCHHC
RSITEAFTKTAQANHSTIVSASEYASDATDFSSQIATIKAKPADALILVGYYAQSADIIK
HHHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHHEEEEEECCCCEEEEEEEECCCHHHHH
QVRQAGITLPIIGSDGLDSPSLVELGGSDVEGVILATDFSRNDPRSIVQNFIREYRNAHG
HHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
IDPDVVASSTYDATNLLIDAFKRSPDSSSSAIRDTLAATQTYEGVTGAIVFTPEREVNKT
CCHHHEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCE
VLFVKIERGAFTFLDKLDPSQLP
EEEEEECCCCEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]