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The map label for this gene is lgrD [H]

Identifier: 159898625

GI number: 159898625

Start: 2630820

End: 2632391

Strand: Reverse

Name: lgrD [H]

Synonym: Haur_2104

Alternate gene names: 159898625

Gene position: 2632391-2630820 (Counterclockwise)

Preceding gene: 159898626

Following gene: 159898624

Centisome position: 41.48

GC content: 49.36

Gene sequence:

>1572_bases
ATGAATTTAATCAATAAGGTTAATAGTGCTGCGGCACTGCCGTTGCGCGAGTATCTTCAGCACCATTTGCCTGAGTCAAT
GCTACCAACGGCATTTGTCGAACTTAGGCAGATTCCACGTTTGCCGAATGGCAAGGTTGATCGGGCAGCCCTGCCAACTA
TCGATTTTCAACAATATAGTGATCCTGAGCACTATGTTGCACCGCGCACTACCACTGAACAGCAGCTTGCTTACATTTGG
CAGCAAACCTTGCGTGTGCCACAGGTGGGGATTCACGATAATTTCTTTGCCTTGGGTGGCGATTCGATTACTAGTATTCA
GGTGGCCGCGCGAGCCAATCAGGCTGGCATTCGGCTGACAGCGCGTCAATTATTTGAGCAACCAACGATTGCCCAACTTG
CTCATGGTGTCGCTATCACCGATCAGCGTAATTTGCTCGATGAAATTGTGCTTGATCCAGCAATCAATCCGCTTGGTCTA
GCTCCCTATCACGAGCACATGCCCCAATCAATCCTGTTGACCGGGGCAACTGGCTTTTTGGGGCCATATTTGTTGGCTGA
ATTGCTCACACAAACATCTGCCCACATCTATTGTGTGGTACGAGCCGCTGATGAGACCCAAGCTTTTGCCAAAATTCGCC
AACAGCTTGAACATGCCAATCGTTGGGAGCCAGCCTTTGCTTCACGGCTCATCGTGATGCTTGGGGATCTGAGCCTTCCG
CAGTTAGGCATGACTGCGGCAGACTGGCAGATGTTGGCTCAAACTATTGATCGGATTTATCACAATGGCGCAGTCGTAAA
TCTGGCGCATTCATATGCCCAACTCAAAGCTAGTAATGTGCAAGCAACCATTGATTTGCTGCGTTTGGCAAGTCAAGTCA
AGCTTAAATCAATGCACTTTAGTTCTACGACCAGTGTTTTTCCAGCAAACACCGCTGCTACAGTACGCTATGAGCAAGAA
CTGCCATCAACACCAGATGGCCTCATCACTGGCTATGCCCAAACCAAGTGGGTTGCTGAACACCTGTTGCTGCAAGCCCG
CGCCCGCGGTATTCCGATTAATTGTTATCGGCCAGGCCGCATTGGTTGGGATACTCAAACGGGTCAGTGGAATCGTGGTG
ATGGTTTGTATCGGTTGCTCCAAGGCTGTTTGCAGTTAGGTCGTGCGCCGTTGGTTGATCGGTTGGCTGAAATTACTCCG
GTTGCTTATGTGGCCCAAGCGATGGTGGCGCTTTCGTTGCAGCCAAGCGGCCAAGGGCAAACGTATCATTTGGTCAACCA
ACAGCATATCGCTTGGAATCAACTGATTAATTGGATGCAAGAACTTGGGTATCCCATTGAACAGGTTGATCTTGATCAGT
GGATGCTGCAATTACAGCGGCAAACCAAGGATGTACCAAATACTTCTCTGCAATCACTGTTAACCTTAGCGCAAGCGGCA
GTGAATGGCCCACATGCTGAGCAAGCCTATGATCAAACTGCGATGCAAGCTGCGCTTGCAGCAACTGAGGTTATGCTACC
AACGCTTGATCGAACAAGCCTTCAACAATGGTTGGCACAACTACGTTTTTAA

Upstream 100 bases:

>100_bases
TTCACCATTTACGGGCAGCCGTCAGGTGCTGGTGGGTATGGCTCATCCAACCACCCATGGCGATGTTGCTAATGCAATCA
ATGGTTAACGGAGCTGAATT

Downstream 100 bases:

>100_bases
CTTTCATATCCATTGTTTCGATAATGCAGGGCTGAGCAAGAAACTGGTTAGCTCTGCGTGACGTTTTGCTAGCAACTTAC
CCATCCTAATTAAAACAGGG

Product: thioester reductase domain-containing protein

Products: NA

Alternate protein names: ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-Leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent glycine adenylase; GlyA; Glycine activase; Linear gramicidin--PCP reductase [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNLINKVNSAAALPLREYLQHHLPESMLPTAFVELRQIPRLPNGKVDRAALPTIDFQQYSDPEHYVAPRTTTEQQLAYIW
QQTLRVPQVGIHDNFFALGGDSITSIQVAARANQAGIRLTARQLFEQPTIAQLAHGVAITDQRNLLDEIVLDPAINPLGL
APYHEHMPQSILLTGATGFLGPYLLAELLTQTSAHIYCVVRAADETQAFAKIRQQLEHANRWEPAFASRLIVMLGDLSLP
QLGMTAADWQMLAQTIDRIYHNGAVVNLAHSYAQLKASNVQATIDLLRLASQVKLKSMHFSSTTSVFPANTAATVRYEQE
LPSTPDGLITGYAQTKWVAEHLLLQARARGIPINCYRPGRIGWDTQTGQWNRGDGLYRLLQGCLQLGRAPLVDRLAEITP
VAYVAQAMVALSLQPSGQGQTYHLVNQQHIAWNQLINWMQELGYPIEQVDLDQWMLQLQRQTKDVPNTSLQSLLTLAQAA
VNGPHAEQAYDQTAMQAALAATEVMLPTLDRTSLQQWLAQLRF

Sequences:

>Translated_523_residues
MNLINKVNSAAALPLREYLQHHLPESMLPTAFVELRQIPRLPNGKVDRAALPTIDFQQYSDPEHYVAPRTTTEQQLAYIW
QQTLRVPQVGIHDNFFALGGDSITSIQVAARANQAGIRLTARQLFEQPTIAQLAHGVAITDQRNLLDEIVLDPAINPLGL
APYHEHMPQSILLTGATGFLGPYLLAELLTQTSAHIYCVVRAADETQAFAKIRQQLEHANRWEPAFASRLIVMLGDLSLP
QLGMTAADWQMLAQTIDRIYHNGAVVNLAHSYAQLKASNVQATIDLLRLASQVKLKSMHFSSTTSVFPANTAATVRYEQE
LPSTPDGLITGYAQTKWVAEHLLLQARARGIPINCYRPGRIGWDTQTGQWNRGDGLYRLLQGCLQLGRAPLVDRLAEITP
VAYVAQAMVALSLQPSGQGQTYHLVNQQHIAWNQLINWMQELGYPIEQVDLDQWMLQLQRQTKDVPNTSLQSLLTLAQAA
VNGPHAEQAYDQTAMQAALAATEVMLPTLDRTSLQQWLAQLRF
>Mature_523_residues
MNLINKVNSAAALPLREYLQHHLPESMLPTAFVELRQIPRLPNGKVDRAALPTIDFQQYSDPEHYVAPRTTTEQQLAYIW
QQTLRVPQVGIHDNFFALGGDSITSIQVAARANQAGIRLTARQLFEQPTIAQLAHGVAITDQRNLLDEIVLDPAINPLGL
APYHEHMPQSILLTGATGFLGPYLLAELLTQTSAHIYCVVRAADETQAFAKIRQQLEHANRWEPAFASRLIVMLGDLSLP
QLGMTAADWQMLAQTIDRIYHNGAVVNLAHSYAQLKASNVQATIDLLRLASQVKLKSMHFSSTTSVFPANTAATVRYEQE
LPSTPDGLITGYAQTKWVAEHLLLQARARGIPINCYRPGRIGWDTQTGQWNRGDGLYRLLQGCLQLGRAPLVDRLAEITP
VAYVAQAMVALSLQPSGQGQTYHLVNQQHIAWNQLINWMQELGYPIEQVDLDQWMLQLQRQTKDVPNTSLQSLLTLAQAA
VNGPHAEQAYDQTAMQAALAATEVMLPTLDRTSLQQWLAQLRF

Specific function: Activates the 13th to the 16th (Trp, D-Leu, Trp and Gly) amino acids in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 14th (D-Leu) amino acid. It also catalyzes

COG id: COG3320

COG function: function code Q; Putative dehydrogenase domain of multifunctional non-ribosomal peptide synthetases and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319591, Length=506, Percent_Identity=28.6561264822134, Blast_Score=203, Evalue=6e-53,
Organism=Drosophila melanogaster, GI28572029, Length=285, Percent_Identity=25.2631578947368, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013120
- InterPro:   IPR016040
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR010080 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF07993 NAD_binding_4; PF00550 PP-binding [H]

EC number: NA

Molecular weight: Translated: 58208; Mature: 58208

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLINKVNSAAALPLREYLQHHLPESMLPTAFVELRQIPRLPNGKVDRAALPTIDFQQYS
CCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCC
DPEHYVAPRTTTEQQLAYIWQQTLRVPQVGIHDNFFALGGDSITSIQVAARANQAGIRLT
CCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEHHCCCCCHHHH
ARQLFEQPTIAQLAHGVAITDQRNLLDEIVLDPAINPLGLAPYHEHMPQSILLTGATGFL
HHHHHCCCCHHHHHCCCEECCHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEECCHHHH
GPYLLAELLTQTSAHIYCVVRAADETQAFAKIRQQLEHANRWEPAFASRLIVMLGDLSLP
HHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
QLGMTAADWQMLAQTIDRIYHNGAVVNLAHSYAQLKASNVQATIDLLRLASQVKLKSMHF
CCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
SSTTSVFPANTAATVRYEQELPSTPDGLITGYAQTKWVAEHLLLQARARGIPINCYRPGR
CCCCCCCCCCCEEEEEEHHHCCCCCCHHEECCHHHHHHHHHHHHHHHHCCCCEEEECCCC
IGWDTQTGQWNRGDGLYRLLQGCLQLGRAPLVDRLAEITPVAYVAQAMVALSLQPSGQGQ
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TYHLVNQQHIAWNQLINWMQELGYPIEQVDLDQWMLQLQRQTKDVPNTSLQSLLTLAQAA
EEEECCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VNGPHAEQAYDQTAMQAALAATEVMLPTLDRTSLQQWLAQLRF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNLINKVNSAAALPLREYLQHHLPESMLPTAFVELRQIPRLPNGKVDRAALPTIDFQQYS
CCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCC
DPEHYVAPRTTTEQQLAYIWQQTLRVPQVGIHDNFFALGGDSITSIQVAARANQAGIRLT
CCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEHHCCCCCHHHH
ARQLFEQPTIAQLAHGVAITDQRNLLDEIVLDPAINPLGLAPYHEHMPQSILLTGATGFL
HHHHHCCCCHHHHHCCCEECCHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEECCHHHH
GPYLLAELLTQTSAHIYCVVRAADETQAFAKIRQQLEHANRWEPAFASRLIVMLGDLSLP
HHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
QLGMTAADWQMLAQTIDRIYHNGAVVNLAHSYAQLKASNVQATIDLLRLASQVKLKSMHF
CCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
SSTTSVFPANTAATVRYEQELPSTPDGLITGYAQTKWVAEHLLLQARARGIPINCYRPGR
CCCCCCCCCCCEEEEEEHHHCCCCCCHHEECCHHHHHHHHHHHHHHHHCCCCEEEECCCC
IGWDTQTGQWNRGDGLYRLLQGCLQLGRAPLVDRLAEITPVAYVAQAMVALSLQPSGQGQ
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TYHLVNQQHIAWNQLINWMQELGYPIEQVDLDQWMLQLQRQTKDVPNTSLQSLLTLAQAA
EEEECCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VNGPHAEQAYDQTAMQAALAATEVMLPTLDRTSLQQWLAQLRF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA