Click here to switch to the map view.

The map label for this gene is phrA [H]

Identifier: 159898493

GI number: 159898493

Start: 2413635

End: 2415095

Strand: Reverse

Name: phrA [H]

Synonym: Haur_1969

Alternate gene names: 159898493

Gene position: 2415095-2413635 (Counterclockwise)

Preceding gene: 159898494

Following gene: 159898491

Centisome position: 38.05

GC content: 52.5

Gene sequence:

>1461_bases
ATGCCTGTGATTTGTTGGTTCCGCCGCGATTTACGCCTAACCGATCATCGCGCCTTGTATGCCGCCGCCGAGGCCAGCGC
TGGGGCAGTTATTCCAGTCTTTATTTTGGATGATACGATTCTGCACGATGGCTATGTTGGAGCAGCCCTGATCGCCGTAA
CGCTTGCCATGCTCGAAGCACTCGATCACGATTTGCAGCAACGAGGCAGTCGTTTGATTGTGCGCCATGGCCAGCCATTA
GCCGAATTGCAACGCCTCGTGAGCGAAACTCAGGCCAGCGGCGTGTACTGGAATCGCGATTATTTGCCTTATGCGATCAA
GCGTGATAGCGCAGTTAAGCACTGGTTACGTGAACAAGGCTTGCAAGCCCATTCGTTTCACGATAGCGTTTTAGTTGAGC
CAGAGGGGCTAAAAACCAAAACTGAGCAAAAACCCTATGTGGTCTATGGATCATATGTCAAGCGCTGGAGTGAATTAGCC
TATCACCAAGCCGAGCAACTTGTGCCCGCCCCCAGCAAATTCGTGGCCCCGCCAAGCGATTTGGCGAGTTTGCCAATTCC
AAGCTTGGCTGATTTGGGCTTTGAGCTACAACAAACGATTCCACAGGTTGGCGAAACAATTGCCCAACAACGTTTGGCGC
AGTTTTTTGATCGGCGGCAGAAACTTTCGGTACTCAAGTATACCAAAGCCCGCGAAGTGCCTGCCGAGGCCGGAACCTCG
CAGCTTTCAGTTGATTTGCGCATGGGCACGATTTCGATTCGCCAATGTTTGAAACAGGCTGTCGATCTGCTGACCGAGCC
ATTAAACGCTGAGCAACGTCAAGGAGTCGATACTTGGCTCAAAGAATTGGCTTGGCGCGATTACTACACCCAATTGATCT
ACCACAACCCATATATGCTCAACGGCTCGCTCGATCCACGCTACGATCAGATCATTTGGCGCAACGATCCAAGTGAGTTT
TTGGCGTGGCAACAGGGCCAAACTGGGTATCCAATTGTTGATGCAGGCCAGCGCCAGCTCAACCAAATGGCGTGGATGCA
TAATCGAGTGCGCATGATCAGCGCCTCATTTTTGATCAAAGATTTGCTGATCGATTGGCGTTGGGGTGAGCGCTATTTTA
TGCAGCAGTTATGTGATGGCGACCCGACCGCCAATAACGGCGGTTGGCAGTGGGCAGCAGGTTCAAGTGGGCCATCAGCC
CAACCCTATTTTCGCATCTTCAACCCAATTGCCCAGAGCAAAAAGCACGACCCAGACGGCCAGTATATTCGGCGATTTGT
GCCCGAATTAGCTAACGTGCCCGATCACTATATTCACGAGCCATGGACCATGCCGCCAGCCGTGCAAGCACATGTTGGCT
GCGTGATTGGGCGCGATTATCCTGCGCCGCTAGTTGAGCATAGTTTTGCCCGTGAACGCGCCTTGGCAGCCTATCGCACA
GCCCTGCAAACCAATGATTAG

Upstream 100 bases:

>100_bases
CCTGAGAACTATAGCGTTTATCTGCCTGTCGCAATCAAAAATTAAACCATCCAACTCCCCAATTGTACGTAGTATGCTGC
GTACAATTGGGGAGTTTTTT

Downstream 100 bases:

>100_bases
GTTACTCAACTTGGCGTTGGCTAGGTCAAGCGCGAAAAAACTCCATATTGCCATCATCGAGCGAGCCAAGCCGCAACATC
AGCAAATCGAGCAGGTAGTT

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MPVICWFRRDLRLTDHRALYAAAEASAGAVIPVFILDDTILHDGYVGAALIAVTLAMLEALDHDLQQRGSRLIVRHGQPL
AELQRLVSETQASGVYWNRDYLPYAIKRDSAVKHWLREQGLQAHSFHDSVLVEPEGLKTKTEQKPYVVYGSYVKRWSELA
YHQAEQLVPAPSKFVAPPSDLASLPIPSLADLGFELQQTIPQVGETIAQQRLAQFFDRRQKLSVLKYTKAREVPAEAGTS
QLSVDLRMGTISIRQCLKQAVDLLTEPLNAEQRQGVDTWLKELAWRDYYTQLIYHNPYMLNGSLDPRYDQIIWRNDPSEF
LAWQQGQTGYPIVDAGQRQLNQMAWMHNRVRMISASFLIKDLLIDWRWGERYFMQQLCDGDPTANNGGWQWAAGSSGPSA
QPYFRIFNPIAQSKKHDPDGQYIRRFVPELANVPDHYIHEPWTMPPAVQAHVGCVIGRDYPAPLVEHSFARERALAAYRT
ALQTND

Sequences:

>Translated_486_residues
MPVICWFRRDLRLTDHRALYAAAEASAGAVIPVFILDDTILHDGYVGAALIAVTLAMLEALDHDLQQRGSRLIVRHGQPL
AELQRLVSETQASGVYWNRDYLPYAIKRDSAVKHWLREQGLQAHSFHDSVLVEPEGLKTKTEQKPYVVYGSYVKRWSELA
YHQAEQLVPAPSKFVAPPSDLASLPIPSLADLGFELQQTIPQVGETIAQQRLAQFFDRRQKLSVLKYTKAREVPAEAGTS
QLSVDLRMGTISIRQCLKQAVDLLTEPLNAEQRQGVDTWLKELAWRDYYTQLIYHNPYMLNGSLDPRYDQIIWRNDPSEF
LAWQQGQTGYPIVDAGQRQLNQMAWMHNRVRMISASFLIKDLLIDWRWGERYFMQQLCDGDPTANNGGWQWAAGSSGPSA
QPYFRIFNPIAQSKKHDPDGQYIRRFVPELANVPDHYIHEPWTMPPAVQAHVGCVIGRDYPAPLVEHSFARERALAAYRT
ALQTND
>Mature_485_residues
PVICWFRRDLRLTDHRALYAAAEASAGAVIPVFILDDTILHDGYVGAALIAVTLAMLEALDHDLQQRGSRLIVRHGQPLA
ELQRLVSETQASGVYWNRDYLPYAIKRDSAVKHWLREQGLQAHSFHDSVLVEPEGLKTKTEQKPYVVYGSYVKRWSELAY
HQAEQLVPAPSKFVAPPSDLASLPIPSLADLGFELQQTIPQVGETIAQQRLAQFFDRRQKLSVLKYTKAREVPAEAGTSQ
LSVDLRMGTISIRQCLKQAVDLLTEPLNAEQRQGVDTWLKELAWRDYYTQLIYHNPYMLNGSLDPRYDQIIWRNDPSEFL
AWQQGQTGYPIVDAGQRQLNQMAWMHNRVRMISASFLIKDLLIDWRWGERYFMQQLCDGDPTANNGGWQWAAGSSGPSAQ
PYFRIFNPIAQSKKHDPDGQYIRRFVPELANVPDHYIHEPWTMPPAVQAHVGCVIGRDYPAPLVEHSFARERALAAYRTA
LQTND

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=479, Percent_Identity=30.2713987473904, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4758072, Length=478, Percent_Identity=30.1255230125523, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI188536103, Length=430, Percent_Identity=29.3023255813954, Blast_Score=192, Evalue=8e-49,
Organism=Escherichia coli, GI1786926, Length=493, Percent_Identity=38.3367139959432, Blast_Score=291, Evalue=5e-80,
Organism=Saccharomyces cerevisiae, GI6324962, Length=529, Percent_Identity=29.8676748582231, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI17137248, Length=500, Percent_Identity=31, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24585455, Length=500, Percent_Identity=31, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24648152, Length=506, Percent_Identity=27.2727272727273, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55387; Mature: 55255

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVICWFRRDLRLTDHRALYAAAEASAGAVIPVFILDDTILHDGYVGAALIAVTLAMLEA
CCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHH
LDHDLQQRGSRLIVRHGQPLAELQRLVSETQASGVYWNRDYLPYAIKRDSAVKHWLREQG
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCEEECCCCCCHHCCCCHHHHHHHHHCC
LQAHSFHDSVLVEPEGLKTKTEQKPYVVYGSYVKRWSELAYHQAEQLVPAPSKFVAPPSD
CCHHCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHH
LASLPIPSLADLGFELQQTIPQVGETIAQQRLAQFFDRRQKLSVLKYTKAREVPAEAGTS
HHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QLSVDLRMGTISIRQCLKQAVDLLTEPLNAEQRQGVDTWLKELAWRDYYTQLIYHNPYML
EEEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEE
NGSLDPRYDQIIWRNDPSEFLAWQQGQTGYPIVDAGQRQLNQMAWMHNRVRMISASFLIK
CCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLLIDWRWGERYFMQQLCDGDPTANNGGWQWAAGSSGPSAQPYFRIFNPIAQSKKHDPDG
HHHHHCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCH
QYIRRFVPELANVPDHYIHEPWTMPPAVQAHVGCVIGRDYPAPLVEHSFARERALAAYRT
HHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQTND
HHCCCC
>Mature Secondary Structure 
PVICWFRRDLRLTDHRALYAAAEASAGAVIPVFILDDTILHDGYVGAALIAVTLAMLEA
CEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHH
LDHDLQQRGSRLIVRHGQPLAELQRLVSETQASGVYWNRDYLPYAIKRDSAVKHWLREQG
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCEEECCCCCCHHCCCCHHHHHHHHHCC
LQAHSFHDSVLVEPEGLKTKTEQKPYVVYGSYVKRWSELAYHQAEQLVPAPSKFVAPPSD
CCHHCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHH
LASLPIPSLADLGFELQQTIPQVGETIAQQRLAQFFDRRQKLSVLKYTKAREVPAEAGTS
HHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QLSVDLRMGTISIRQCLKQAVDLLTEPLNAEQRQGVDTWLKELAWRDYYTQLIYHNPYML
EEEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEE
NGSLDPRYDQIIWRNDPSEFLAWQQGQTGYPIVDAGQRQLNQMAWMHNRVRMISASFLIK
CCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLLIDWRWGERYFMQQLCDGDPTANNGGWQWAAGSSGPSAQPYFRIFNPIAQSKKHDPDG
HHHHHCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCH
QYIRRFVPELANVPDHYIHEPWTMPPAVQAHVGCVIGRDYPAPLVEHSFARERALAAYRT
HHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQTND
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]