Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 159898428

GI number: 159898428

Start: 2298422

End: 2299435

Strand: Direct

Name: prfB [H]

Synonym: Haur_1904

Alternate gene names: 159898428

Gene position: 2298422-2299435 (Clockwise)

Preceding gene: 159898426

Following gene: 159898429

Centisome position: 36.22

GC content: 50.39

Gene sequence:

>1014_bases
TTGCATGACTTAGAACATCAGGCTGCCGAACCAAGCTTGTGGGATAATCCACAACAGGCCCAAAGTATTTTGCAACGACT
CGCTCGCATGCAAGAAGAATTGAAAATGTGGGATAGTTTAGCCGTCGATTTGGCAACCCTGCGCGATCTGTTGCAACTTT
CGGCTGATGATGCTGATTCGCTCAGCCAAATTGAGCGTGAGGTCAACGGCTTGACCAAATCGGTCGATCAGCTTGAATTG
AGCATTTTGCTGGGCGAAAAATACGATAGCACCAATGCTTTTATCTCGATTCAATCGGGCGCTGGCGGAGTCGAATCACA
GGATTGGGCGCAGATGTTGTTGCGTATGTATAGCCGTTGGGGCGAAAGCCAGGGTTTTCGTACCACGATTGTCGATATGA
TGGACGGCGAAGAGGCAGGCATTAAAAGTGCTACCTTAGAATTGCGCGGCCCCTATGCTTATGGCTATGCCAAGGCCGAG
GCTGGCATTCATCGTTTAGTTCGGCTTTCGCCTTTCGATGCCAACCATCGTCGCCATACCTCGTTTGCCCGGGTTGAGGT
GTTGCCCGAAGTCGATGATGCCGCCGAAGTCAAAATTAATCCTGAAGATCTGCGGGTTGATGTCTATCGGGCTGGTGGCC
ATGGTGGGCAAGGTGTTAATACAACTGACTCAGCGGTGCGTTTGGTTTATCGTGGTGGCACGCCCGATGAAATTATCGTA
ACCTGTCAAAATGAACGTTCGCAATTGCAAAATAAAGAAACCGCGATGAATGTGTTGCGGGCGCGTTTGTTGGAACGTGA
GCTAGCCCGCCAAGTTGCTGAACGTGCCAAACTCAAGGGCGAACATCGCGATGCCGCTTGGGGCAATCAAATTCGTTCGT
ATGTGCTTGACGATCGGCGAGTTAAAGATCATCGGACCAACGTCGAAACATCGAATACCCAAGCAGTGCTCGATGGCGAG
ATTGATTTATTTATCGATGCCTTCTTGCGCTGGAGAACCGAAGGCACAGAGTAG

Upstream 100 bases:

>100_bases
ATGACAGAGTTTATTGATCTTCGGGCGGCCCTAAACTCGTTGCAAGCGCGATTCGATGCGCTAAGGGGGCGGCTTTGACT
GGGCTGCCAAACAAGCCGAA

Downstream 100 bases:

>100_bases
TGAGGTTTATATGCGACGAGTAGTGTTGTGGATGCTAGCATGTTTGTTTGTGCTGGGGTTTCAGGCTACGGCTCAGGCGG
CTGAGCCAGCCAAAATCTCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MHDLEHQAAEPSLWDNPQQAQSILQRLARMQEELKMWDSLAVDLATLRDLLQLSADDADSLSQIEREVNGLTKSVDQLEL
SILLGEKYDSTNAFISIQSGAGGVESQDWAQMLLRMYSRWGESQGFRTTIVDMMDGEEAGIKSATLELRGPYAYGYAKAE
AGIHRLVRLSPFDANHRRHTSFARVEVLPEVDDAAEVKINPEDLRVDVYRAGGHGGQGVNTTDSAVRLVYRGGTPDEIIV
TCQNERSQLQNKETAMNVLRARLLERELARQVAERAKLKGEHRDAAWGNQIRSYVLDDRRVKDHRTNVETSNTQAVLDGE
IDLFIDAFLRWRTEGTE

Sequences:

>Translated_337_residues
MHDLEHQAAEPSLWDNPQQAQSILQRLARMQEELKMWDSLAVDLATLRDLLQLSADDADSLSQIEREVNGLTKSVDQLEL
SILLGEKYDSTNAFISIQSGAGGVESQDWAQMLLRMYSRWGESQGFRTTIVDMMDGEEAGIKSATLELRGPYAYGYAKAE
AGIHRLVRLSPFDANHRRHTSFARVEVLPEVDDAAEVKINPEDLRVDVYRAGGHGGQGVNTTDSAVRLVYRGGTPDEIIV
TCQNERSQLQNKETAMNVLRARLLERELARQVAERAKLKGEHRDAAWGNQIRSYVLDDRRVKDHRTNVETSNTQAVLDGE
IDLFIDAFLRWRTEGTE
>Mature_337_residues
MHDLEHQAAEPSLWDNPQQAQSILQRLARMQEELKMWDSLAVDLATLRDLLQLSADDADSLSQIEREVNGLTKSVDQLEL
SILLGEKYDSTNAFISIQSGAGGVESQDWAQMLLRMYSRWGESQGFRTTIVDMMDGEEAGIKSATLELRGPYAYGYAKAE
AGIHRLVRLSPFDANHRRHTSFARVEVLPEVDDAAEVKINPEDLRVDVYRAGGHGGQGVNTTDSAVRLVYRGGTPDEIIV
TCQNERSQLQNKETAMNVLRARLLERELARQVAERAKLKGEHRDAAWGNQIRSYVLDDRRVKDHRTNVETSNTQAVLDGE
IDLFIDAFLRWRTEGTE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=33.6734693877551, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI34577120, Length=306, Percent_Identity=32.3529411764706, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI166795305, Length=189, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=45.7831325301205, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=39.0946502057613, Blast_Score=171, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17542784, Length=326, Percent_Identity=25.7668711656442, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6321295, Length=302, Percent_Identity=31.7880794701987, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI19921226, Length=228, Percent_Identity=35.5263157894737, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37978; Mature: 37978

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDLEHQAAEPSLWDNPQQAQSILQRLARMQEELKMWDSLAVDLATLRDLLQLSADDADS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSQIEREVNGLTKSVDQLELSILLGEKYDSTNAFISIQSGAGGVESQDWAQMLLRMYSRW
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
GESQGFRTTIVDMMDGEEAGIKSATLELRGPYAYGYAKAEAGIHRLVRLSPFDANHRRHT
CCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEECHHHHHHHHHHHHEECCCCCCCCCCC
SFARVEVLPEVDDAAEVKINPEDLRVDVYRAGGHGGQGVNTTDSAVRLVYRGGTPDEIIV
CHHEEEECCCCCCCCEEEECHHHEEEEEEECCCCCCCCCCCCHHHEEEEECCCCCCEEEE
TCQNERSQLQNKETAMNVLRARLLERELARQVAERAKLKGEHRDAAWGNQIRSYVLDDRR
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
VKDHRTNVETSNTQAVLDGEIDLFIDAFLRWRTEGTE
HHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHDLEHQAAEPSLWDNPQQAQSILQRLARMQEELKMWDSLAVDLATLRDLLQLSADDADS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSQIEREVNGLTKSVDQLELSILLGEKYDSTNAFISIQSGAGGVESQDWAQMLLRMYSRW
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
GESQGFRTTIVDMMDGEEAGIKSATLELRGPYAYGYAKAEAGIHRLVRLSPFDANHRRHT
CCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEECHHHHHHHHHHHHEECCCCCCCCCCC
SFARVEVLPEVDDAAEVKINPEDLRVDVYRAGGHGGQGVNTTDSAVRLVYRGGTPDEIIV
CHHEEEECCCCCCCCEEEECHHHEEEEEEECCCCCCCCCCCCHHHEEEEECCCCCCEEEE
TCQNERSQLQNKETAMNVLRARLLERELARQVAERAKLKGEHRDAAWGNQIRSYVLDDRR
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
VKDHRTNVETSNTQAVLDGEIDLFIDAFLRWRTEGTE
HHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA